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The map label for this gene is tgt

Identifier: 74318093

GI number: 74318093

Start: 2168196

End: 2169299

Strand: Direct

Name: tgt

Synonym: Tbd_2075

Alternate gene names: 74318093

Gene position: 2168196-2169299 (Clockwise)

Preceding gene: 74318092

Following gene: 74318094

Centisome position: 74.51

GC content: 66.3

Gene sequence:

>1104_bases
ATGAAATTCGACCTCCTCCGCACCGACGGCGGCGCACGGCGCGGCCAACTCCATCTCGCGCACGGGGTCGTGCAGACGCC
GGTGTTCATGCCGGTCGGCACCTACGGCACGGTGAAGGCGATGTCGCCGACGGAAATCGCCGACATCGGCTTCGAGATGC
TGCTGTCCAACACCTTCCACCTGTGGCTGCGGCCGGGGCTCGAGGTGATCGAGGCACACGGCGGGCTGCACCGCTTCATG
GGCTGGGACAAGCCGATCCTCACCGACTCGGGGGGCTTTCAGGTGTTCAGCCTCGGCAAGCTCAGAAAGATCACCGAGGA
GGGCGTGAAATTCGCCTCGCCGACCAACGGCGACAAGCTCTTTCTGACGCCCGAAACCTCGATGCAGATCCAGCGCACGC
TCAATTCCGACATCGTCATGATCTTCGACGAGTGCACGCCCTACCCCGCGACGGAACGGCAGGCGGCCGATTCGATGCGG
ATGAGCCTGAGATGGGCGGCGCGCTCGAAGGCGGCGCACGCGGGCAATCCGAACGCGCTGTACGGGATCGTCCAGGGCGG
CATGTACGAAGCCCTGCGCGACGAGTCGGCGCGCGAACTGATCGGCATGGACTTCGACGGCTATGCGATCGGCGGCCTCT
CGGTCGGCGAGCCGAAGGACGACATGACGCGCATTCTCGCGCACACCGCGCCGCAGCTTCCCGCCGACAAGCCGCGCTAC
CTGATGGGCGTGGGGACGCCGTCCGATCTCGTTGCGGCGGTGGCGGCCGGCATCGACCAGTTCGACTGCGTGCTGCCGAC
GCGCAACGCGCGCCACGGCATCCTGTTCACGCGCCGCGGCGAAATCCGCATTCGCAATGCACGCTGGAAGCTCGACACCG
CGCCGATCGACGAAGAATGCGACTGCTACGCCTGCCGCCATTTCACGCGCGCCTACGTGCACCATCTGATTCGCGCCGGC
GAGATCCTCGGCGCACGGCTCACCACCTTGCACAACCTGCATTACTACCACCGGCTGATGGCCGAGGTGCGCGCGGCAAT
CGACGCACAGCGCTTTTCGGAATTCGTCGCCCGCTTCCACGCGACGCAGGCGCTCGGATGGTGA

Upstream 100 bases:

>100_bases
CGATCCGCCGCGCCTACGCGCACGCGATCGCCGAGCGCTATCGCTTCTTCAGTTACGGCGACGCGATGTTCCTCGAAAAA
GCCAGTCTGGAAACGCCCGC

Downstream 100 bases:

>100_bases
CGGCCGTGGCCTGACAGAAATTCTTCCCCCGGGGTTTGTCAAGAATCGTCCCGGTGCTGCCGTTGACGCACCCACGACAT
AACGACAAACCACTCAGGGA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKFDLLRTDGGARRGQLHLAHGVVQTPVFMPVGTYGTVKAMSPTEIADIGFEMLLSNTFHLWLRPGLEVIEAHGGLHRFM
GWDKPILTDSGGFQVFSLGKLRKITEEGVKFASPTNGDKLFLTPETSMQIQRTLNSDIVMIFDECTPYPATERQAADSMR
MSLRWAARSKAAHAGNPNALYGIVQGGMYEALRDESARELIGMDFDGYAIGGLSVGEPKDDMTRILAHTAPQLPADKPRY
LMGVGTPSDLVAAVAAGIDQFDCVLPTRNARHGILFTRRGEIRIRNARWKLDTAPIDEECDCYACRHFTRAYVHHLIRAG
EILGARLTTLHNLHYYHRLMAEVRAAIDAQRFSEFVARFHATQALGW

Sequences:

>Translated_367_residues
MKFDLLRTDGGARRGQLHLAHGVVQTPVFMPVGTYGTVKAMSPTEIADIGFEMLLSNTFHLWLRPGLEVIEAHGGLHRFM
GWDKPILTDSGGFQVFSLGKLRKITEEGVKFASPTNGDKLFLTPETSMQIQRTLNSDIVMIFDECTPYPATERQAADSMR
MSLRWAARSKAAHAGNPNALYGIVQGGMYEALRDESARELIGMDFDGYAIGGLSVGEPKDDMTRILAHTAPQLPADKPRY
LMGVGTPSDLVAAVAAGIDQFDCVLPTRNARHGILFTRRGEIRIRNARWKLDTAPIDEECDCYACRHFTRAYVHHLIRAG
EILGARLTTLHNLHYYHRLMAEVRAAIDAQRFSEFVARFHATQALGW
>Mature_367_residues
MKFDLLRTDGGARRGQLHLAHGVVQTPVFMPVGTYGTVKAMSPTEIADIGFEMLLSNTFHLWLRPGLEVIEAHGGLHRFM
GWDKPILTDSGGFQVFSLGKLRKITEEGVKFASPTNGDKLFLTPETSMQIQRTLNSDIVMIFDECTPYPATERQAADSMR
MSLRWAARSKAAHAGNPNALYGIVQGGMYEALRDESARELIGMDFDGYAIGGLSVGEPKDDMTRILAHTAPQLPADKPRY
LMGVGTPSDLVAAVAAGIDQFDCVLPTRNARHGILFTRRGEIRIRNARWKLDTAPIDEECDCYACRHFTRAYVHHLIRAG
EILGARLTTLHNLHYYHRLMAEVRAAIDAQRFSEFVARFHATQALGW

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=349, Percent_Identity=44.9856733524355, Blast_Score=309, Evalue=2e-84,
Organism=Escherichia coli, GI1786607, Length=366, Percent_Identity=63.1147540983607, Blast_Score=487, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17544680, Length=360, Percent_Identity=44.7222222222222, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI25146502, Length=177, Percent_Identity=25.4237288135593, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920510, Length=351, Percent_Identity=45.8689458689459, Blast_Score=324, Evalue=7e-89,
Organism=Drosophila melanogaster, GI21355311, Length=165, Percent_Identity=30.3030303030303, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_THIDA (Q3SH61)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315833.1
- HSSP:   P28720
- ProteinModelPortal:   Q3SH61
- SMR:   Q3SH61
- STRING:   Q3SH61
- GeneID:   3672247
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2075
- NMPDR:   fig|292415.3.peg.2230
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q3SH61
- BioCyc:   TDEN292415:TBD_2075-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 40855; Mature: 40855

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDLLRTDGGARRGQLHLAHGVVQTPVFMPVGTYGTVKAMSPTEIADIGFEMLLSNTFH
CCEEEEECCCCCCCCEEEEECCHHHCCEEEECCCCCEEECCCCCHHHHHHHHHHHCCCEE
LWLRPGLEVIEAHGGLHRFMGWDKPILTDSGGFQVFSLGKLRKITEEGVKFASPTNGDKL
EEECCCHHHHHHCCCHHHHCCCCCCEEECCCCEEEEECHHHHHHHHHCCEECCCCCCCEE
FLTPETSMQIQRTLNSDIVMIFDECTPYPATERQAADSMRMSLRWAARSKAAHAGNPNAL
EECCCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
YGIVQGGMYEALRDESARELIGMDFDGYAIGGLSVGEPKDDMTRILAHTAPQLPADKPRY
EEECCCCHHHHHHCHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCE
LMGVGTPSDLVAAVAAGIDQFDCVLPTRNARHGILFTRRGEIRIRNARWKLDTAPIDEEC
EEECCCHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCEEEECCEEEECCCCCCCCC
DCYACRHFTRAYVHHLIRAGEILGARLTTLHNLHYYHRLMAEVRAAIDAQRFSEFVARFH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATQALGW
HHHHCCC
>Mature Secondary Structure
MKFDLLRTDGGARRGQLHLAHGVVQTPVFMPVGTYGTVKAMSPTEIADIGFEMLLSNTFH
CCEEEEECCCCCCCCEEEEECCHHHCCEEEECCCCCEEECCCCCHHHHHHHHHHHCCCEE
LWLRPGLEVIEAHGGLHRFMGWDKPILTDSGGFQVFSLGKLRKITEEGVKFASPTNGDKL
EEECCCHHHHHHCCCHHHHCCCCCCEEECCCCEEEEECHHHHHHHHHCCEECCCCCCCEE
FLTPETSMQIQRTLNSDIVMIFDECTPYPATERQAADSMRMSLRWAARSKAAHAGNPNAL
EECCCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
YGIVQGGMYEALRDESARELIGMDFDGYAIGGLSVGEPKDDMTRILAHTAPQLPADKPRY
EEECCCCHHHHHHCHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCE
LMGVGTPSDLVAAVAAGIDQFDCVLPTRNARHGILFTRRGEIRIRNARWKLDTAPIDEEC
EEECCCHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCEEEECCEEEECCCCCCCCC
DCYACRHFTRAYVHHLIRAGEILGARLTTLHNLHYYHRLMAEVRAAIDAQRFSEFVARFH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATQALGW
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA