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The map label for this gene is queA

Identifier: 74318092

GI number: 74318092

Start: 2167165

End: 2168199

Strand: Direct

Name: queA

Synonym: Tbd_2074

Alternate gene names: 74318092

Gene position: 2167165-2168199 (Clockwise)

Preceding gene: 74318084

Following gene: 74318093

Centisome position: 74.48

GC content: 69.28

Gene sequence:

>1035_bases
ATGCGTGTCGCCGATTTCGACTTCGAACTTCCCCCGGATCTCATTGCGCAGTTCCCGCCCGACGTCCGCGGCGCAAGCCG
GCTGCTGCACGTCACGGCGGCGGGGGCGCTTCACGATCGCATGTTTCGCGAGCTGCCAACGCTGCTCGGCGCCGACGATC
TGCTCGTCATGAACGACACGCGCGTGATCAAGGCGCGGCTTTTCGGCGAGAAGGATTCGGGCGGCAGGGTCGAACTTCTG
GTCGAGCGCGTGACCGGCGAATTCGAGGCGCTGGCGTTCATCCGCGCCAGCCACGCACCCAAACCGGGCGCGCGCATCCG
CCTCGCCGACGATGTCGCGCTCGACGTCCTCGACAAGCAGCACGACCTGACGCGCCTGCATTTTCCTGCGCCGGTGCTCG
ACGTGCTCGACCGCTGCGGCCGGCTGCCGCTGCCGCCCTATATCGAACACGCGCCGACCGAGGAAGACGAAGCGCGTTAC
CAAACCGTGTACGCGAACGAACCCGGCGCGGTGGCGGCGCCGACGGCGGGGCTTCATTTCGACGCGGCGATGCTCGGGAC
ACTGCAGACGCAGGGCGTGCGGACGGCACGGGTGACGCTGCACGTCGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGCCG
AGGTTGCCGACCACGTGATGCACAGCGAGCGCTACACGGTCCCCGCCGCCACGGTCGCGGCGATCGCCGAGACGCGGGCG
CGCGGCGGGCGGGTCGTCGCGGTCGGGACGACGAGCCTGCGCGCACTCGAGGCGGCTTCCGCGTCAGGCTCGGTCGAGGC
CGGCGTCGGCGAGACCGCGATCTTCATCACGCCGGGCTACCGCTTCCGCACGGTCGACGCGCTCGTCACCAATTTCCACC
TGCCGCGCTCGACCCTGCTGATGCTGGTCTCGGCGTTCAGCGGCATCGAGACGATCCGCCGCGCCTACGCGCACGCGATC
GCCGAGCGCTATCGCTTCTTCAGTTACGGCGACGCGATGTTCCTCGAAAAAGCCAGTCTGGAAACGCCCGCATGA

Upstream 100 bases:

>100_bases
CCCGGCCGGGTGCCGGACGCGAACGCGTCCGGCGACAGAAGCCGGGCATTATCGCCGAATCGGGCGAACGCCCGCAAGGC
GACCGCTATAATCGCGGCCC

Downstream 100 bases:

>100_bases
AATTCGACCTCCTCCGCACCGACGGCGGCGCACGGCGCGGCCAACTCCATCTCGCGCACGGGGTCGTGCAGACGCCGGTG
TTCATGCCGGTCGGCACCTA

Product: queuosine biosynthesis protein

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRVADFDFELPPDLIAQFPPDVRGASRLLHVTAAGALHDRMFRELPTLLGADDLLVMNDTRVIKARLFGEKDSGGRVELL
VERVTGEFEALAFIRASHAPKPGARIRLADDVALDVLDKQHDLTRLHFPAPVLDVLDRCGRLPLPPYIEHAPTEEDEARY
QTVYANEPGAVAAPTAGLHFDAAMLGTLQTQGVRTARVTLHVGAGTFQPVRVAEVADHVMHSERYTVPAATVAAIAETRA
RGGRVVAVGTTSLRALEAASASGSVEAGVGETAIFITPGYRFRTVDALVTNFHLPRSTLLMLVSAFSGIETIRRAYAHAI
AERYRFFSYGDAMFLEKASLETPA

Sequences:

>Translated_344_residues
MRVADFDFELPPDLIAQFPPDVRGASRLLHVTAAGALHDRMFRELPTLLGADDLLVMNDTRVIKARLFGEKDSGGRVELL
VERVTGEFEALAFIRASHAPKPGARIRLADDVALDVLDKQHDLTRLHFPAPVLDVLDRCGRLPLPPYIEHAPTEEDEARY
QTVYANEPGAVAAPTAGLHFDAAMLGTLQTQGVRTARVTLHVGAGTFQPVRVAEVADHVMHSERYTVPAATVAAIAETRA
RGGRVVAVGTTSLRALEAASASGSVEAGVGETAIFITPGYRFRTVDALVTNFHLPRSTLLMLVSAFSGIETIRRAYAHAI
AERYRFFSYGDAMFLEKASLETPA
>Mature_344_residues
MRVADFDFELPPDLIAQFPPDVRGASRLLHVTAAGALHDRMFRELPTLLGADDLLVMNDTRVIKARLFGEKDSGGRVELL
VERVTGEFEALAFIRASHAPKPGARIRLADDVALDVLDKQHDLTRLHFPAPVLDVLDRCGRLPLPPYIEHAPTEEDEARY
QTVYANEPGAVAAPTAGLHFDAAMLGTLQTQGVRTARVTLHVGAGTFQPVRVAEVADHVMHSERYTVPAATVAAIAETRA
RGGRVVAVGTTSLRALEAASASGSVEAGVGETAIFITPGYRFRTVDALVTNFHLPRSTLLMLVSAFSGIETIRRAYAHAI
AERYRFFSYGDAMFLEKASLETPA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=343, Percent_Identity=53.3527696793003, Blast_Score=339, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_THIDA (Q3SH62)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315832.1
- ProteinModelPortal:   Q3SH62
- SMR:   Q3SH62
- STRING:   Q3SH62
- GeneID:   3672246
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2074
- NMPDR:   fig|292415.3.peg.2229
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   Q3SH62
- BioCyc:   TDEN292415:TBD_2074-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37332; Mature: 37332

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVADFDFELPPDLIAQFPPDVRGASRLLHVTAAGALHDRMFRELPTLLGADDLLVMNDT
CCCCCCCCCCCHHHHHHCCCCCCCHHHEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCC
RVIKARLFGEKDSGGRVELLVERVTGEFEALAFIRASHAPKPGARIRLADDVALDVLDKQ
EEEEEEECCCCCCCCEEEEEEEHHCCCHHHHHHHHHCCCCCCCCEEEEECHHHHHHHCCC
HDLTRLHFPAPVLDVLDRCGRLPLPPYIEHAPTEEDEARYQTVYANEPGAVAAPTAGLHF
CCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCEE
DAAMLGTLQTQGVRTARVTLHVGAGTFQPVRVAEVADHVMHSERYTVPAATVAAIAETRA
HHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
RGGRVVAVGTTSLRALEAASASGSVEAGVGETAIFITPGYRFRTVDALVTNFHLPRSTLL
CCCEEEEEECCHHHHHHHCCCCCCEECCCCCEEEEEECCCCEEHHHHHHHHCCCCHHHHH
MLVSAFSGIETIRRAYAHAIAERYRFFSYGDAMFLEKASLETPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCCC
>Mature Secondary Structure
MRVADFDFELPPDLIAQFPPDVRGASRLLHVTAAGALHDRMFRELPTLLGADDLLVMNDT
CCCCCCCCCCCHHHHHHCCCCCCCHHHEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCC
RVIKARLFGEKDSGGRVELLVERVTGEFEALAFIRASHAPKPGARIRLADDVALDVLDKQ
EEEEEEECCCCCCCCEEEEEEEHHCCCHHHHHHHHHCCCCCCCCEEEEECHHHHHHHCCC
HDLTRLHFPAPVLDVLDRCGRLPLPPYIEHAPTEEDEARYQTVYANEPGAVAAPTAGLHF
CCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCEE
DAAMLGTLQTQGVRTARVTLHVGAGTFQPVRVAEVADHVMHSERYTVPAATVAAIAETRA
HHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
RGGRVVAVGTTSLRALEAASASGSVEAGVGETAIFITPGYRFRTVDALVTNFHLPRSTLL
CCCEEEEEECCHHHHHHHCCCCCCEECCCCCEEEEEECCCCEEHHHHHHHHCCCCHHHHH
MLVSAFSGIETIRRAYAHAIAERYRFFSYGDAMFLEKASLETPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA