Click here to switch to the map view.

The map label for this gene is dnaB [H]

Identifier: 74318086

GI number: 74318086

Start: 2161338

End: 2162738

Strand: Reverse

Name: dnaB [H]

Synonym: Tbd_2068

Alternate gene names: 74318086

Gene position: 2162738-2161338 (Counterclockwise)

Preceding gene: 74318087

Following gene: 74318085

Centisome position: 74.33

GC content: 65.6

Gene sequence:

>1401_bases
ATGAACGCCCATGACCTCAACCAGCCTCCGCACTCCGTCGACGCCGAACAGGCAGTGCTCGGCGGTCTGCTGCTCGACAA
CGGCGCATGGGACCGCATCGCCGACCTGCTGACGCCGGCGGATTTCTACCTCGCCGACCATCGCCTCATGTTTGAAACCA
TGCGCGACATGTTCGAGCGCGGCGAACCGGTTGACGTTGTTACCCTTGCGGCTCGAATAGAGGCCCGTGGTGAAGCGCAA
AAGGTCGGGTTGGGATACGTGGCCACCTTGTCCACGCAAACGCCCTCAGCGGCCAACATTGTCCGTTATGCCGGAATCGT
GACGGAGAAGCGGGCCCGGCGCGATCTGCTCGCAGCTGGTCACCGCATCGCCGGCATGGCGAGCACCGATGGCGGTGATG
CCGGCGATGCCATCCAGGAAGCCCAGGCGCTGGTGATGGCACTCACCGAAGGGCGCAGCGCCGGCAGCGAGCCGCAAGCG
GTCGACGATCTGCTGCCGGCCGTGCTGCGGAGTATCGACGAACGCTTCCACAAAGCCGGCGAGTTTTCCGGCGTCGCCTC
GGGCTATGGCGATCTCGACCGCCTAACCTGCGGCCTGCAGCCGGGCGACCTGGTCATCGTGGCCGGACGCCCAAGCATGG
GCAAAACCACATTGGCGGTGAACATCGCCGAGAACGTCGCGGCCGATGGCGGCGTGGCCTTGGTCTTCTCCCTGGAGATG
GGCGCCCGCCAACTGGTCGAGCGGTCGGTCTGCCGCTTCGGCGGGATCTCGACCACAGCCGTTCGCAACGGCGACATGCG
TCAGGAAGATTTCGAACGCCTGTTGGTTGCGCACAAACGCTTGCAGGGCAAGCGCCTGGTGATCGATGACACACCGTCGA
CCTCGCCCGCCCGGATGCGTGCCAAGGCGCGCAAGGTCAAACACCGCTATGGCCGGCTCGATTTGGTGGTGATCGACTAC
CTGCAGCTGATGGCCGGCGATGGCAACACCCGGAGCGAACAGCTCGGGGGACTGACTCGCGCGCTCAAGCTGATGGCCCG
TGAGCTGGGGGCCCCTGTCGTCCTGCTGTCGCAGCTGAACCGCGGGCTCGAGCAGCGCACCGACAAGCGCCCGGTGCTCT
CCGACCTGCGCGAATCTGGCGCGATCGAACAGGACGCCGACGTTGTGCTGATGGTGCACCGCCCCGACTACTACGACGCC
GACAGCCCGCACACGGGACTCGCCGAGGTGCTGATCCGCAAGCAGCGCATGGGGCCGCTCGGCACCGTCACGCTGTGCTT
TGAGGGCGAGTTCTCACGATTCCACGACGTCGATCATGACGCACTGCGGGAGGCGAAGGCCCACGCCTATACGGCTAGCA
ATCCACGTCCTACCCGGCGTAAGGGGGGCTTCGATGACTAA

Upstream 100 bases:

>100_bases
CTGATAGCCGCAAGCCGTTCACTGCCTCGCTCGAGTGGTTGCTGAGGCCCGAGAACTTCGCCAAGGTCCGCGAAGGCCGA
TACCACGACGGGGGCCAGGC

Downstream 100 bases:

>100_bases
GCGCATTCATGGCACCTCCCTAGGCCGCATCGATGCTGTTCCGCCCTGGTGCTACTGGCGCCCGGGCATGGGCCCGGAAG
CTGCCGCACTCGAGGCCGCG

Product: DnaB helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MNAHDLNQPPHSVDAEQAVLGGLLLDNGAWDRIADLLTPADFYLADHRLMFETMRDMFERGEPVDVVTLAARIEARGEAQ
KVGLGYVATLSTQTPSAANIVRYAGIVTEKRARRDLLAAGHRIAGMASTDGGDAGDAIQEAQALVMALTEGRSAGSEPQA
VDDLLPAVLRSIDERFHKAGEFSGVASGYGDLDRLTCGLQPGDLVIVAGRPSMGKTTLAVNIAENVAADGGVALVFSLEM
GARQLVERSVCRFGGISTTAVRNGDMRQEDFERLLVAHKRLQGKRLVIDDTPSTSPARMRAKARKVKHRYGRLDLVVIDY
LQLMAGDGNTRSEQLGGLTRALKLMARELGAPVVLLSQLNRGLEQRTDKRPVLSDLRESGAIEQDADVVLMVHRPDYYDA
DSPHTGLAEVLIRKQRMGPLGTVTLCFEGEFSRFHDVDHDALREAKAHAYTASNPRPTRRKGGFDD

Sequences:

>Translated_466_residues
MNAHDLNQPPHSVDAEQAVLGGLLLDNGAWDRIADLLTPADFYLADHRLMFETMRDMFERGEPVDVVTLAARIEARGEAQ
KVGLGYVATLSTQTPSAANIVRYAGIVTEKRARRDLLAAGHRIAGMASTDGGDAGDAIQEAQALVMALTEGRSAGSEPQA
VDDLLPAVLRSIDERFHKAGEFSGVASGYGDLDRLTCGLQPGDLVIVAGRPSMGKTTLAVNIAENVAADGGVALVFSLEM
GARQLVERSVCRFGGISTTAVRNGDMRQEDFERLLVAHKRLQGKRLVIDDTPSTSPARMRAKARKVKHRYGRLDLVVIDY
LQLMAGDGNTRSEQLGGLTRALKLMARELGAPVVLLSQLNRGLEQRTDKRPVLSDLRESGAIEQDADVVLMVHRPDYYDA
DSPHTGLAEVLIRKQRMGPLGTVTLCFEGEFSRFHDVDHDALREAKAHAYTASNPRPTRRKGGFDD
>Mature_466_residues
MNAHDLNQPPHSVDAEQAVLGGLLLDNGAWDRIADLLTPADFYLADHRLMFETMRDMFERGEPVDVVTLAARIEARGEAQ
KVGLGYVATLSTQTPSAANIVRYAGIVTEKRARRDLLAAGHRIAGMASTDGGDAGDAIQEAQALVMALTEGRSAGSEPQA
VDDLLPAVLRSIDERFHKAGEFSGVASGYGDLDRLTCGLQPGDLVIVAGRPSMGKTTLAVNIAENVAADGGVALVFSLEM
GARQLVERSVCRFGGISTTAVRNGDMRQEDFERLLVAHKRLQGKRLVIDDTPSTSPARMRAKARKVKHRYGRLDLVVIDY
LQLMAGDGNTRSEQLGGLTRALKLMARELGAPVVLLSQLNRGLEQRTDKRPVLSDLRESGAIEQDADVVLMVHRPDYYDA
DSPHTGLAEVLIRKQRMGPLGTVTLCFEGEFSRFHDVDHDALREAKAHAYTASNPRPTRRKGGFDD

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=439, Percent_Identity=43.5079726651481, Blast_Score=357, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50610; Mature: 50610

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAHDLNQPPHSVDAEQAVLGGLLLDNGAWDRIADLLTPADFYLADHRLMFETMRDMFER
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
GEPVDVVTLAARIEARGEAQKVGLGYVATLSTQTPSAANIVRYAGIVTEKRARRDLLAAG
CCCCCEEHHHHHHHHCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
HRIAGMASTDGGDAGDAIQEAQALVMALTEGRSAGSEPQAVDDLLPAVLRSIDERFHKAG
CHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
EFSGVASGYGDLDRLTCGLQPGDLVIVAGRPSMGKTTLAVNIAENVAADGGVALVFSLEM
CCCCCCCCCCCHHHHCCCCCCCCEEEEECCCCCCCEEEEEEEHHHHCCCCCEEEEEEEHH
GARQLVERSVCRFGGISTTAVRNGDMRQEDFERLLVAHKRLQGKRLVIDDTPSTSPARMR
HHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
AKARKVKHRYGRLDLVVIDYLQLMAGDGNTRSEQLGGLTRALKLMARELGAPVVLLSQLN
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RGLEQRTDKRPVLSDLRESGAIEQDADVVLMVHRPDYYDADSPHTGLAEVLIRKQRMGPL
HHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCC
GTVTLCFEGEFSRFHDVDHDALREAKAHAYTASNPRPTRRKGGFDD
CEEEEEECCCCHHHHCCCHHHHHHHHHHHEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAHDLNQPPHSVDAEQAVLGGLLLDNGAWDRIADLLTPADFYLADHRLMFETMRDMFER
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
GEPVDVVTLAARIEARGEAQKVGLGYVATLSTQTPSAANIVRYAGIVTEKRARRDLLAAG
CCCCCEEHHHHHHHHCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
HRIAGMASTDGGDAGDAIQEAQALVMALTEGRSAGSEPQAVDDLLPAVLRSIDERFHKAG
CHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
EFSGVASGYGDLDRLTCGLQPGDLVIVAGRPSMGKTTLAVNIAENVAADGGVALVFSLEM
CCCCCCCCCCCHHHHCCCCCCCCEEEEECCCCCCCEEEEEEEHHHHCCCCCEEEEEEEHH
GARQLVERSVCRFGGISTTAVRNGDMRQEDFERLLVAHKRLQGKRLVIDDTPSTSPARMR
HHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
AKARKVKHRYGRLDLVVIDYLQLMAGDGNTRSEQLGGLTRALKLMARELGAPVVLLSQLN
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RGLEQRTDKRPVLSDLRESGAIEQDADVVLMVHRPDYYDADSPHTGLAEVLIRKQRMGPL
HHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCC
GTVTLCFEGEFSRFHDVDHDALREAKAHAYTASNPRPTRRKGGFDD
CEEEEEECCCCHHHHCCCHHHHHHHHHHHEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]