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The map label for this gene is ppnK

Identifier: 74318049

GI number: 74318049

Start: 2122157

End: 2123029

Strand: Reverse

Name: ppnK

Synonym: Tbd_2031

Alternate gene names: 74318049

Gene position: 2123029-2122157 (Counterclockwise)

Preceding gene: 74318054

Following gene: 74318048

Centisome position: 72.96

GC content: 66.67

Gene sequence:

>873_bases
ATGCAGATTCCTTTCCACACCGTCGGCCTCGTCGGCAAATACGACAACCAGGGCATGGAGGCCTCGGTCCGCGCGCTTGC
CGAATTCCTGCGCGGACGCGGGCACGCCGTCGTGCTCGCGTGCCAGACGGCGGAACATTTCGGCATCACCGATTTCCCGA
CGCGCAACCTGCACGACCTCGCGACCGAGAGCGACGCCGTCGTCGTGTTGGGGGGGGACGGCACGATGCTCTCGATCGCG
CGCGAACTCTCGGCCCACGGCGTGCCGTTGATCGGCATCAACCAGGGCCGGCTCGGCTTTCTGACCGACATCACCGTCGA
CCACATGTACGACGCGGTCGACGAGATCCTGAGCGGGCAATACGTCGCCGAAGAACGCATCCTGCTCAAGGGACAGATCC
TGCGCGGCGGCGAGCGGGTCTTTGAGGCGACCGCATTCAACGACGTCGTCGTCGGCAAGGGCGGCTCGGGGCGGCTGATC
GACCTCGAAATCGCGATCGACGGCGAGTTCGTCTACAGCCAGCGTGCCGACGGCCTGGTCGTCACGACCCCGACCGGAAC
GACCGCGTATGCCTTGTCCGCCGGCGGGCCGATCGTGCACCCGACCCTCGAAGCGGTCGCGCTCGTGCCGATCTGCCCGC
ATACGCTGTCGGCGCGGCCGATCGTCGTCAGCGGCCGTTCGCGCATCGAGCTGCACCTGACCTACGCCGACGACGCGCGC
GTCCACTTCGACGGCCAGCATCACTTCGACCTGCAAAGCGGCGACCACGTCTGGATCACGCGGGCGAACCGCCCGATCAC
GCTGCTGCATCCGCATTCCTACAGCTACTACGACACCCTGCGGCAGAAGCTGCACTGGGGCAAGAAGCTCTGA

Upstream 100 bases:

>100_bases
AGGGACGGCGGCTCCGGTCAGACCCGCTTCGGCCGCGGCCAGCGAGGCGGCACACGCGCGGGTCCGGAATCCTCGTCCGG
CCCCTTTATAATCCTTCCCC

Downstream 100 bases:

>100_bases
GCGAGAAGATGGCCGTCCCGAGCGATCCTTGCGCGCACGCGGTGCCCGGCTGCGATGCGGGCGCCGCAAAGCTGGCCGGA
CGTCGGCCGCAGGGAGACTG

Product: NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MQIPFHTVGLVGKYDNQGMEASVRALAEFLRGRGHAVVLACQTAEHFGITDFPTRNLHDLATESDAVVVLGGDGTMLSIA
RELSAHGVPLIGINQGRLGFLTDITVDHMYDAVDEILSGQYVAEERILLKGQILRGGERVFEATAFNDVVVGKGGSGRLI
DLEIAIDGEFVYSQRADGLVVTTPTGTTAYALSAGGPIVHPTLEAVALVPICPHTLSARPIVVSGRSRIELHLTYADDAR
VHFDGQHHFDLQSGDHVWITRANRPITLLHPHSYSYYDTLRQKLHWGKKL

Sequences:

>Translated_290_residues
MQIPFHTVGLVGKYDNQGMEASVRALAEFLRGRGHAVVLACQTAEHFGITDFPTRNLHDLATESDAVVVLGGDGTMLSIA
RELSAHGVPLIGINQGRLGFLTDITVDHMYDAVDEILSGQYVAEERILLKGQILRGGERVFEATAFNDVVVGKGGSGRLI
DLEIAIDGEFVYSQRADGLVVTTPTGTTAYALSAGGPIVHPTLEAVALVPICPHTLSARPIVVSGRSRIELHLTYADDAR
VHFDGQHHFDLQSGDHVWITRANRPITLLHPHSYSYYDTLRQKLHWGKKL
>Mature_290_residues
MQIPFHTVGLVGKYDNQGMEASVRALAEFLRGRGHAVVLACQTAEHFGITDFPTRNLHDLATESDAVVVLGGDGTMLSIA
RELSAHGVPLIGINQGRLGFLTDITVDHMYDAVDEILSGQYVAEERILLKGQILRGGERVFEATAFNDVVVGKGGSGRLI
DLEIAIDGEFVYSQRADGLVVTTPTGTTAYALSAGGPIVHPTLEAVALVPICPHTLSARPIVVSGRSRIELHLTYADDAR
VHFDGQHHFDLQSGDHVWITRANRPITLLHPHSYSYYDTLRQKLHWGKKL

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=262, Percent_Identity=31.2977099236641, Blast_Score=121, Evalue=7e-28,
Organism=Escherichia coli, GI1788968, Length=287, Percent_Identity=40.0696864111498, Blast_Score=229, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322509, Length=240, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320794, Length=242, Percent_Identity=28.9256198347107, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6325068, Length=219, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=6e-28,
Organism=Drosophila melanogaster, GI20129957, Length=278, Percent_Identity=30.9352517985612, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI281363321, Length=278, Percent_Identity=30.9352517985612, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI281363323, Length=278, Percent_Identity=30.9352517985612, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24653422, Length=278, Percent_Identity=30.9352517985612, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24653424, Length=278, Percent_Identity=30.9352517985612, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28573832, Length=253, Percent_Identity=32.0158102766798, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI161077047, Length=253, Percent_Identity=32.0158102766798, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI28573830, Length=253, Percent_Identity=32.0158102766798, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI28573828, Length=253, Percent_Identity=32.0158102766798, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI28573826, Length=253, Percent_Identity=32.0158102766798, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_THIDA (Q3SHA5)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315789.1
- ProteinModelPortal:   Q3SHA5
- SMR:   Q3SHA5
- STRING:   Q3SHA5
- GeneID:   3672202
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2031
- NMPDR:   fig|292415.3.peg.2171
- eggNOG:   COG0061
- HOGENOM:   HBG713904
- OMA:   FESFMID
- PhylomeDB:   Q3SHA5
- BioCyc:   TDEN292415:TBD_2031-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 31603; Mature: 31603

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIPFHTVGLVGKYDNQGMEASVRALAEFLRGRGHAVVLACQTAEHFGITDFPTRNLHDL
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHH
ATESDAVVVLGGDGTMLSIARELSAHGVPLIGINQGRLGFLTDITVDHMYDAVDEILSGQ
CCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECHHHHHHHHHHHHHCCC
YVAEERILLKGQILRGGERVFEATAFNDVVVGKGGSGRLIDLEIAIDGEFVYSQRADGLV
EEECCEEEEEEEEECCCCCEEEEECCCCEEEECCCCCCEEEEEEEECCEEEEECCCCCEE
VTTPTGTTAYALSAGGPIVHPTLEAVALVPICPHTLSARPIVVSGRSRIELHLTYADDAR
EECCCCCEEEEEECCCCEECCCHHEEEEEEECCCCCCCCEEEEECCCEEEEEEEECCCCE
VHFDGQHHFDLQSGDHVWITRANRPITLLHPHSYSYYDTLRQKLHWGKKL
EEECCCEEEECCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQIPFHTVGLVGKYDNQGMEASVRALAEFLRGRGHAVVLACQTAEHFGITDFPTRNLHDL
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHH
ATESDAVVVLGGDGTMLSIARELSAHGVPLIGINQGRLGFLTDITVDHMYDAVDEILSGQ
CCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECHHHHHHHHHHHHHCCC
YVAEERILLKGQILRGGERVFEATAFNDVVVGKGGSGRLIDLEIAIDGEFVYSQRADGLV
EEECCEEEEEEEEECCCCCEEEEECCCCEEEECCCCCCEEEEEEEECCEEEEECCCCCEE
VTTPTGTTAYALSAGGPIVHPTLEAVALVPICPHTLSARPIVVSGRSRIELHLTYADDAR
EECCCCCEEEEEECCCCEECCCHHEEEEEEECCCCCCCCEEEEECCCEEEEEEEECCCCE
VHFDGQHHFDLQSGDHVWITRANRPITLLHPHSYSYYDTLRQKLHWGKKL
EEECCCEEEECCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA