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The map label for this gene is glcD [H]

Identifier: 74318016

GI number: 74318016

Start: 2085606

End: 2086988

Strand: Direct

Name: glcD [H]

Synonym: Tbd_1998

Alternate gene names: 74318016

Gene position: 2085606-2086988 (Clockwise)

Preceding gene: 74318015

Following gene: 74318018

Centisome position: 71.68

GC content: 69.85

Gene sequence:

>1383_bases
ATGCTGTCCGACACCGCGCTTGCCGCCTTCCGTTCCGCGCTCGGGGGCGAGCGCGTATTCGACGACGCCGCGACGCGCGC
GCTCTATGCGAGCGACGAGACCCCGCGCCGGGTAGACCCCGACGCCGTTCTGTTTCCCGGCTCGCACGACGACGTGGTCG
CGCTCGTGCGCGTCGCAGTCGAGCACCGCGTCGCGATAACGCCGCGCGGCGCCGGCTCCGGCAACGTCGGCGGCGCGCTG
CCCTCGCCGGGCAGCGTCGTCGTCAGCTTCGAGTGCATGCGCTGGGTGAAGGAATTCGACCCGGTCAATCGCCTCGTCGT
CGTCGAGCCCGGCGTGGTCACGGAGGAGATCGATCGCCTCGCGCGCAGCGCCGGGCTCTTCTATCCGCCCGATCCCGGCA
GCGCCGCCTACAGCCGCATCGGCGGCAACCTCGCGATGAACGCCGCCGGACCGCGCGCCGTCAAATACGGCGTCACGCGC
GACTACGTGCTCGGCCTGCGCGCGGTGACCGGCACCGGCGAGGAAATCTGCACCGGCTGCCGCACCACGAAGGGTGTGAC
CGGCTACGACCTGACGCGTCTGCTCGTCGGCTCGGGCGGCACGCTCGCCCTCGTCACCGAAGCCACGCTCAAGCTGCTGC
CAGCGCCCGAAGGCGTCGCGACGCTGCGCGTGTGCTTCGCGAGCAACCACGCCGCGCTCGAGGCGGTCAGCCGGGTGATG
CGCCAGGCGGTGACACCGTGTGCGCTCGAATTCATGGACCAGCATGCGATCGACGCGATCCGCCCCACCGGCGCCGCGGA
CGATCTCCCGGACGGCACGCGCGCGCTCCTGATGGTCGAGGCCGACGGCCGGGCGGACGACCTCCCCCGCCAGATCGAAG
CGCTCGAGCATGCCCTCGCCGGCGACGGCATGCTCGAAATGCGCAGCGGTTTCGACCGCGACGCGATCGCTCAGCTCTGG
GCAGCGCGGCGTTCGCTCTCGCATGCGGTCAAACGCATCGCCCCGCTCAAGCTCAACGAGGACGTCGTCGTGCCGGTGTC
GGCTCTCGCCGACTTCGTCGATTTCATCGATCACGTCGCCGGCGCGCAACGCTTGCCGATCGTATCCTTCGGCCACGCCG
GCAACGGCAACCTGCACGTCAACGTGATGGTCGATCCTGACGACGGGGACGCGCTCGCGCGCGCCCACCTCGCCCTCGAA
TCGATCATGCTGCGCGTGATCGCACTCGGTGGCACGCTCTCGGGCGAGCACGGAATCGGCACCGAGAAGCGCAAGTTCGT
GCCGCTCGAAATCGCGCCCGCGACGCTCGAGTTGATGCGCGCCATCAAGCGTCAGTTCGATCCGCACGGTCTGCTCAACC
CCGGCAAGCTTTTTCCCGATTGA

Upstream 100 bases:

>100_bases
TGGGACATCGGGATGGATTTCGACGCCGAGATGATCGGCCAGTTGATTCTGCAGCTCCCCCCGGAAAACCCGGCCTGACC
CTTACGTCTTTGCTTCCGCC

Downstream 100 bases:

>100_bases
TAATTGCCGCAAACGCTTTACAAGCGCGCAAGGCATCTATAGAATGCGCGGCTTCGTTGGGGGTATAGCTCAGCTGGGAG
AGCGCTTGCATGGCATGCAA

Product: oxidoreductase, FAD-binding

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MLSDTALAAFRSALGGERVFDDAATRALYASDETPRRVDPDAVLFPGSHDDVVALVRVAVEHRVAITPRGAGSGNVGGAL
PSPGSVVVSFECMRWVKEFDPVNRLVVVEPGVVTEEIDRLARSAGLFYPPDPGSAAYSRIGGNLAMNAAGPRAVKYGVTR
DYVLGLRAVTGTGEEICTGCRTTKGVTGYDLTRLLVGSGGTLALVTEATLKLLPAPEGVATLRVCFASNHAALEAVSRVM
RQAVTPCALEFMDQHAIDAIRPTGAADDLPDGTRALLMVEADGRADDLPRQIEALEHALAGDGMLEMRSGFDRDAIAQLW
AARRSLSHAVKRIAPLKLNEDVVVPVSALADFVDFIDHVAGAQRLPIVSFGHAGNGNLHVNVMVDPDDGDALARAHLALE
SIMLRVIALGGTLSGEHGIGTEKRKFVPLEIAPATLELMRAIKRQFDPHGLLNPGKLFPD

Sequences:

>Translated_460_residues
MLSDTALAAFRSALGGERVFDDAATRALYASDETPRRVDPDAVLFPGSHDDVVALVRVAVEHRVAITPRGAGSGNVGGAL
PSPGSVVVSFECMRWVKEFDPVNRLVVVEPGVVTEEIDRLARSAGLFYPPDPGSAAYSRIGGNLAMNAAGPRAVKYGVTR
DYVLGLRAVTGTGEEICTGCRTTKGVTGYDLTRLLVGSGGTLALVTEATLKLLPAPEGVATLRVCFASNHAALEAVSRVM
RQAVTPCALEFMDQHAIDAIRPTGAADDLPDGTRALLMVEADGRADDLPRQIEALEHALAGDGMLEMRSGFDRDAIAQLW
AARRSLSHAVKRIAPLKLNEDVVVPVSALADFVDFIDHVAGAQRLPIVSFGHAGNGNLHVNVMVDPDDGDALARAHLALE
SIMLRVIALGGTLSGEHGIGTEKRKFVPLEIAPATLELMRAIKRQFDPHGLLNPGKLFPD
>Mature_460_residues
MLSDTALAAFRSALGGERVFDDAATRALYASDETPRRVDPDAVLFPGSHDDVVALVRVAVEHRVAITPRGAGSGNVGGAL
PSPGSVVVSFECMRWVKEFDPVNRLVVVEPGVVTEEIDRLARSAGLFYPPDPGSAAYSRIGGNLAMNAAGPRAVKYGVTR
DYVLGLRAVTGTGEEICTGCRTTKGVTGYDLTRLLVGSGGTLALVTEATLKLLPAPEGVATLRVCFASNHAALEAVSRVM
RQAVTPCALEFMDQHAIDAIRPTGAADDLPDGTRALLMVEADGRADDLPRQIEALEHALAGDGMLEMRSGFDRDAIAQLW
AARRSLSHAVKRIAPLKLNEDVVVPVSALADFVDFIDHVAGAQRLPIVSFGHAGNGNLHVNVMVDPDDGDALARAHLALE
SIMLRVIALGGTLSGEHGIGTEKRKFVPLEIAPATLELMRAIKRQFDPHGLLNPGKLFPD

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=456, Percent_Identity=34.2105263157895, Blast_Score=235, Evalue=8e-62,
Organism=Homo sapiens, GI37595754, Length=479, Percent_Identity=32.5678496868476, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI119964728, Length=465, Percent_Identity=30.1075268817204, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI4501993, Length=259, Percent_Identity=28.957528957529, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1789351, Length=461, Percent_Identity=32.3210412147505, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1787977, Length=515, Percent_Identity=27.1844660194175, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI48994914, Length=156, Percent_Identity=34.6153846153846, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994907, Length=439, Percent_Identity=22.5512528473804, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71992373, Length=468, Percent_Identity=28.6324786324786, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71992381, Length=468, Percent_Identity=27.7777777777778, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71992392, Length=371, Percent_Identity=30.7277628032345, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17534361, Length=468, Percent_Identity=27.7777777777778, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17556096, Length=223, Percent_Identity=28.6995515695067, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320023, Length=483, Percent_Identity=26.2939958592132, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6320027, Length=433, Percent_Identity=26.5588914549654, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6320764, Length=488, Percent_Identity=26.844262295082, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI18921117, Length=438, Percent_Identity=28.9954337899543, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24639277, Length=438, Percent_Identity=28.9954337899543, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24639275, Length=438, Percent_Identity=28.9954337899543, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653753, Length=470, Percent_Identity=25.531914893617, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.1.28

Molecular weight: Translated: 48730; Mature: 48730

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDTALAAFRSALGGERVFDDAATRALYASDETPRRVDPDAVLFPGSHDDVVALVRVAV
CCCHHHHHHHHHHCCCCHHHHHHHHHEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHH
EHRVAITPRGAGSGNVGGALPSPGSVVVSFECMRWVKEFDPVNRLVVVEPGVVTEEIDRL
HHEEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCEEEEECCCCHHHHHHHH
ARSAGLFYPPDPGSAAYSRIGGNLAMNAAGPRAVKYGVTRDYVLGLRAVTGTGEEICTGC
HHHCCCCCCCCCCHHHHHHCCCCEEECCCCCCCEEECCCHHHHHEEEEECCCCHHHHHCC
RTTKGVTGYDLTRLLVGSGGTLALVTEATLKLLPAPEGVATLRVCFASNHAALEAVSRVM
CCCCCCCCHHHHHHEECCCCCEEEEEEHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHH
RQAVTPCALEFMDQHAIDAIRPTGAADDLPDGTRALLMVEADGRADDLPRQIEALEHALA
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHC
GDGMLEMRSGFDRDAIAQLWAARRSLSHAVKRIAPLKLNEDVVVPVSALADFVDFIDHVA
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHC
GAQRLPIVSFGHAGNGNLHVNVMVDPDDGDALARAHLALESIMLRVIALGGTLSGEHGIG
CCCCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TEKRKFVPLEIAPATLELMRAIKRQFDPHGLLNPGKLFPD
CCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLSDTALAAFRSALGGERVFDDAATRALYASDETPRRVDPDAVLFPGSHDDVVALVRVAV
CCCHHHHHHHHHHCCCCHHHHHHHHHEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHH
EHRVAITPRGAGSGNVGGALPSPGSVVVSFECMRWVKEFDPVNRLVVVEPGVVTEEIDRL
HHEEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCEEEEECCCCHHHHHHHH
ARSAGLFYPPDPGSAAYSRIGGNLAMNAAGPRAVKYGVTRDYVLGLRAVTGTGEEICTGC
HHHCCCCCCCCCCHHHHHHCCCCEEECCCCCCCEEECCCHHHHHEEEEECCCCHHHHHCC
RTTKGVTGYDLTRLLVGSGGTLALVTEATLKLLPAPEGVATLRVCFASNHAALEAVSRVM
CCCCCCCCHHHHHHEECCCCCEEEEEEHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHH
RQAVTPCALEFMDQHAIDAIRPTGAADDLPDGTRALLMVEADGRADDLPRQIEALEHALA
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHC
GDGMLEMRSGFDRDAIAQLWAARRSLSHAVKRIAPLKLNEDVVVPVSALADFVDFIDHVA
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHC
GAQRLPIVSFGHAGNGNLHVNVMVDPDDGDALARAHLALESIMLRVIALGGTLSGEHGIG
CCCCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TEKRKFVPLEIAPATLELMRAIKRQFDPHGLLNPGKLFPD
CCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]