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The map label for this gene is leuB

Identifier: 74317938

GI number: 74317938

Start: 2016826

End: 2017887

Strand: Reverse

Name: leuB

Synonym: Tbd_1920

Alternate gene names: 74317938

Gene position: 2017887-2016826 (Counterclockwise)

Preceding gene: 74317939

Following gene: 74317937

Centisome position: 69.35

GC content: 64.69

Gene sequence:

>1062_bases
ATGAAGATTGCAGTTTTGCCGGGTGACGGCATCGGTCCGGAAATCATCTCCCAGGCAGTCAAGGTGCTGGAGGCGCTCAA
GTCCGAAGGCGCGAAGATCGAGATGGAAACCGCGCCGATCGGCGGCGCCGGCTACGACGCGGCGGGCGATCCGCTGCCGG
AGGCGACGCTGAAACTCGCGCGCGAAGCCGACGCGGTGCTGCTCGGCGCCGTGGGGGGGCCGCAGTACGACACGCTCGAT
CGCCCGCTGCGCCCGGAACGCGGCCTGCTGCGCATTCGCAAGGAACTGAACCTCTTCGCCAACCTGCGCCCGGCACTCTT
GTATCCCGAACTCGCGTCGGCGTCGTCGCTGAAGCCCGAGGTCGTGTCCGGCCTCGATCTGATGATCGTGCGCGAACTGA
CCGGCGATGTGTACTTCGGCCAGCCGCGCGGCATCGAAACACGGAATGGCGAGCGCGTCGGCTTCAACACCATGCTGTAT
TCGGAGTCCGAGGTGCGCCGCGTCGCCCACGTCGCCTTCGGCATCGCGATGAAGCGCGGGAAGAAGCTCTGCTCGGTCGA
AAAGGCCAACGTGCTCGAATGCTCGGAGCTGTGGAAGGAAATCATGATCGAGGTGGCCAAGGACTACCCCGAGGTCGAGC
TTTCGCACATGTACGTCGACAACGCCGCGATGCAGCTCATCCGCGCCCCCAAGCAGTTCGACACCATCGTCACCGGCAAC
ATCTTTGGCGACATCCTGTCCGACGCCGCCTCGATGCTGTCGGGATCGATCGGCATGCTGCCGTCGGCCTCGCTCGACGA
ACACAACAAGGGCATGTACGAGCCGATCCACGGCTCGGCGCCCGACATCGCCGGGAAGAACCTCGCCAACCCGCTCGCGA
CGATCCTGTCGGTCGCGATGATGTACCGCTACACCTTCGCCGATCTGGCGACTGCCGACCGCATCGAGAACGCAGTCAAG
CAGGTGATCGCGAAGGGCTATCGCACCGGCGACATCTGGACCGAGGGTACGCAGAAGGTGTCGTGCAGCGAGATGGGCGA
CGCGGTGGTCGTGGCACTTTGA

Upstream 100 bases:

>100_bases
CTACGAAGCGCGGCGCAGGCAGGAAGCGCCGTGGTTGTTTGCGTGAATTTTTCATCCGTGAATGCCCGAAGGGAATTCGC
GGAAAAAGGGTTTTGAGAAC

Downstream 100 bases:

>100_bases
TTTCTAGGGGGCAGGATTCGGGATCCAGGGGGCAGGGAACGAGCGGCCACAGGCCGCGCAATTTTCTCGGCGCGGGCTTT
GCCCGCACCACCCCTGAATC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKIAVLPGDGIGPEIISQAVKVLEALKSEGAKIEMETAPIGGAGYDAAGDPLPEATLKLAREADAVLLGAVGGPQYDTLD
RPLRPERGLLRIRKELNLFANLRPALLYPELASASSLKPEVVSGLDLMIVRELTGDVYFGQPRGIETRNGERVGFNTMLY
SESEVRRVAHVAFGIAMKRGKKLCSVEKANVLECSELWKEIMIEVAKDYPEVELSHMYVDNAAMQLIRAPKQFDTIVTGN
IFGDILSDAASMLSGSIGMLPSASLDEHNKGMYEPIHGSAPDIAGKNLANPLATILSVAMMYRYTFADLATADRIENAVK
QVIAKGYRTGDIWTEGTQKVSCSEMGDAVVVAL

Sequences:

>Translated_353_residues
MKIAVLPGDGIGPEIISQAVKVLEALKSEGAKIEMETAPIGGAGYDAAGDPLPEATLKLAREADAVLLGAVGGPQYDTLD
RPLRPERGLLRIRKELNLFANLRPALLYPELASASSLKPEVVSGLDLMIVRELTGDVYFGQPRGIETRNGERVGFNTMLY
SESEVRRVAHVAFGIAMKRGKKLCSVEKANVLECSELWKEIMIEVAKDYPEVELSHMYVDNAAMQLIRAPKQFDTIVTGN
IFGDILSDAASMLSGSIGMLPSASLDEHNKGMYEPIHGSAPDIAGKNLANPLATILSVAMMYRYTFADLATADRIENAVK
QVIAKGYRTGDIWTEGTQKVSCSEMGDAVVVAL
>Mature_353_residues
MKIAVLPGDGIGPEIISQAVKVLEALKSEGAKIEMETAPIGGAGYDAAGDPLPEATLKLAREADAVLLGAVGGPQYDTLD
RPLRPERGLLRIRKELNLFANLRPALLYPELASASSLKPEVVSGLDLMIVRELTGDVYFGQPRGIETRNGERVGFNTMLY
SESEVRRVAHVAFGIAMKRGKKLCSVEKANVLECSELWKEIMIEVAKDYPEVELSHMYVDNAAMQLIRAPKQFDTIVTGN
IFGDILSDAASMLSGSIGMLPSASLDEHNKGMYEPIHGSAPDIAGKNLANPLATILSVAMMYRYTFADLATADRIENAVK
QVIAKGYRTGDIWTEGTQKVSCSEMGDAVVVAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=329, Percent_Identity=31.6109422492401, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI28178816, Length=365, Percent_Identity=27.9452054794521, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI28178821, Length=316, Percent_Identity=29.1139240506329, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI4758582, Length=343, Percent_Identity=27.4052478134111, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI28178838, Length=302, Percent_Identity=27.4834437086093, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI28178819, Length=150, Percent_Identity=34, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87081683, Length=351, Percent_Identity=50.997150997151, Blast_Score=353, Evalue=7e-99,
Organism=Escherichia coli, GI1788101, Length=342, Percent_Identity=33.0409356725146, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1787381, Length=399, Percent_Identity=26.0651629072682, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71986051, Length=341, Percent_Identity=34.3108504398827, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25144293, Length=344, Percent_Identity=29.9418604651163, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17550882, Length=355, Percent_Identity=29.8591549295775, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=333, Percent_Identity=27.9279279279279, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=360, Percent_Identity=44.1666666666667, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6322097, Length=349, Percent_Identity=36.1031518624642, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324709, Length=363, Percent_Identity=33.6088154269972, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324291, Length=333, Percent_Identity=30.6306306306306, Blast_Score=122, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24643268, Length=362, Percent_Identity=33.1491712707182, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24643270, Length=362, Percent_Identity=33.1491712707182, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24661184, Length=365, Percent_Identity=30.958904109589, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI161078633, Length=334, Percent_Identity=29.6407185628743, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24650122, Length=334, Percent_Identity=29.6407185628743, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI161078637, Length=334, Percent_Identity=29.6407185628743, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI161078635, Length=334, Percent_Identity=29.6407185628743, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078639, Length=332, Percent_Identity=29.8192771084337, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281362242, Length=347, Percent_Identity=26.2247838616715, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24648872, Length=347, Percent_Identity=26.2247838616715, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI20130355, Length=361, Percent_Identity=25.207756232687, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_THIDA (Q3SHL3)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315678.1
- ProteinModelPortal:   Q3SHL3
- SMR:   Q3SHL3
- STRING:   Q3SHL3
- GeneID:   3674030
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1920
- NMPDR:   fig|292415.3.peg.2017
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   NTMIYSE
- PhylomeDB:   Q3SHL3
- ProtClustDB:   PRK00772
- BioCyc:   TDEN292415:TBD_1920-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38082; Mature: 38082

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 93-93 BINDING 103-103 BINDING 131-131 BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVLPGDGIGPEIISQAVKVLEALKSEGAKIEMETAPIGGAGYDAAGDPLPEATLKLA
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHH
READAVLLGAVGGPQYDTLDRPLRPERGLLRIRKELNLFANLRPALLYPELASASSLKPE
HHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCHHCCCCCCHH
VVSGLDLMIVRELTGDVYFGQPRGIETRNGERVGFNTMLYSESEVRRVAHVAFGIAMKRG
HHCCCHHHHHHHHCCCEEECCCCCCCCCCCCEECCHHEEECHHHHHHHHHHHHHHHHHHC
KKLCSVEKANVLECSELWKEIMIEVAKDYPEVELSHMYVDNAAMQLIRAPKQFDTIVTGN
CHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHEEHHHHHHHHHHCHHHHHHEEEHH
IFGDILSDAASMLSGSIGMLPSASLDEHNKGMYEPIHGSAPDIAGKNLANPLATILSVAM
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
MYRYTFADLATADRIENAVKQVIAKGYRTGDIWTEGTQKVSCSEMGDAVVVAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHCCCEEEEEC
>Mature Secondary Structure
MKIAVLPGDGIGPEIISQAVKVLEALKSEGAKIEMETAPIGGAGYDAAGDPLPEATLKLA
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHH
READAVLLGAVGGPQYDTLDRPLRPERGLLRIRKELNLFANLRPALLYPELASASSLKPE
HHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCHHCCCCCCHH
VVSGLDLMIVRELTGDVYFGQPRGIETRNGERVGFNTMLYSESEVRRVAHVAFGIAMKRG
HHCCCHHHHHHHHCCCEEECCCCCCCCCCCCEECCHHEEECHHHHHHHHHHHHHHHHHHC
KKLCSVEKANVLECSELWKEIMIEVAKDYPEVELSHMYVDNAAMQLIRAPKQFDTIVTGN
CHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHEEHHHHHHHHHHCHHHHHHEEEHH
IFGDILSDAASMLSGSIGMLPSASLDEHNKGMYEPIHGSAPDIAGKNLANPLATILSVAM
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
MYRYTFADLATADRIENAVKQVIAKGYRTGDIWTEGTQKVSCSEMGDAVVVAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA