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The map label for this gene is purF [H]

Identifier: 74317926

GI number: 74317926

Start: 2004113

End: 2005633

Strand: Reverse

Name: purF [H]

Synonym: Tbd_1908

Alternate gene names: 74317926

Gene position: 2005633-2004113 (Counterclockwise)

Preceding gene: 74317927

Following gene: 74317925

Centisome position: 68.93

GC content: 65.22

Gene sequence:

>1521_bases
ATGTGCGGGGTAATCGGAGTCGTCGCCAACACGGCGGTCAATCAGCTTTTGTACGACGGGCTGATGGTGTTGCAGCATCG
CGGACAGGATGCTGCGGGCATCGTGACCGCCGAGGAAAACCGTTTTCACATGCACAAGGCGCCCGGGCTCGCGCGCGACG
TCTTCCGCACACGCGACATGCGCAACCTGCTCGGCGACATGGGCGTCGCACACGTGCGCTATCCCACCGCGGGCAGTGCG
TCGTCGTCGGCCGAATCGCAGCCGTTCTACGTGAACTCGCCCTACGGCATCGTGCTCGGGCACAACGGCAACCTGACGAA
CACGCATGAACTGAAGGAATCGTTGTTCCGCAGCGATCTGCGCCACGTCAACACCAACTCCGACTCCGAGGTCCTGCTCA
ATGTACTGGCGCACGAGCTCCACGTCCGCGCGTCCGGGCAGCAGCTGAGCCTCGATACGATCTTCGGCGCGGTCGCCGGC
GTCCACGCGCGCTGCAAGGGCGCCTACGCCGTGACCGCGTTCATCGCCGGCTACGGCCTGCTGGCCTTCCGCGATCCGCA
CGGCATCCGCCCGCTGGTGATCGGCATCAACGACCAGGTCACGCCGCACGAGTACATCGTCGCGTCCGAGAGCGTCGCGA
TCGATACGCTCGGCTTCCGCCTGCTGCGCGACGTCGCGCCCGGCGAGGCCGTGTTCATCGACTACGAGCGTCGGCTGCAC
AGCCGCCAGTGCAGCGACAACCCCGTGCTCAACCCCTGCATTTTCGAATACGTCTATCTGGCCCGCCCGGATTCGGTCAT
GGACGGAATCTCGGTCTACAGCACGCGCCTCGCGATGGGCGTGTCGCTCGCGGAAAAAATCCGCCGCGAATTCGGCCACC
TGCAGATCGACGTCGTGATCCCGATCCCCGACACCAGCCGCCCGTCGGCGCTTCAATTGGCGAACCATCTCGCGGTGCCG
TACCGTGAGGGCTTCATCAAGAACCGCTACATCGGCCGCACTTTCATCATGCCGGGGCAGGCGCTGCGCAAGAAGTCGGT
GCGGCAGAAGCTCAACGCGATCGCCGAGGAGTTCAAGGGCAAGAACGTCCTGCTCGTCGACGATTCGATCGTCCGCGGCA
CGACGAGCCGGGAGATTGTCCAGATGGCGCGCGACGCGGGTGCGGAGAAGGTCTATTTCGCGTCCGCCTCGCCGCCTGTG
CGCTTCTGTAACGTCTACGGCATCGACATGCCGTCGCGCCGCGAACTCATTGCCAACGGCCGCAATGACGAGGAAATCGC
GCGTGAGATCGGCTGCGACGCGCTGATCTATCAGGATCTCGACGCGATGGTCGAGGTGGTGCGCGCCGCCAACCCGGCGA
TCCGCGAGTTCGACACCTCGTGCTTCAACGGCTGCTACATCACCGGCGACATCACGCCCGAGTACCTCGACTACGTCGAG
GCCGCGCGCGACGGCGAAAGCCCGCCGCCGTCGGCGCTGTCGACCCAGCAGCTCGACCTCAACCTCGCGACGGGCAGTTG
A

Upstream 100 bases:

>100_bases
GGGCACCCTGCAGTCGATGGCGCAGAGCGCCCTGCCGCTGGTGCCGGCAAGCGTCGCGAAATACGTTCGCTTTGAATAAC
TTTATGGATGGGTGCACGGT

Downstream 100 bases:

>100_bases
CCGCCACAGTCCCCGGGCGTAGATTCGGCCGGACGCGCCGATTTGAATCCCAGCTTGCGGGCGCTTGAAAAATACGGCTA
AAGCGAGGCGACGGAAACCC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MCGVIGVVANTAVNQLLYDGLMVLQHRGQDAAGIVTAEENRFHMHKAPGLARDVFRTRDMRNLLGDMGVAHVRYPTAGSA
SSSAESQPFYVNSPYGIVLGHNGNLTNTHELKESLFRSDLRHVNTNSDSEVLLNVLAHELHVRASGQQLSLDTIFGAVAG
VHARCKGAYAVTAFIAGYGLLAFRDPHGIRPLVIGINDQVTPHEYIVASESVAIDTLGFRLLRDVAPGEAVFIDYERRLH
SRQCSDNPVLNPCIFEYVYLARPDSVMDGISVYSTRLAMGVSLAEKIRREFGHLQIDVVIPIPDTSRPSALQLANHLAVP
YREGFIKNRYIGRTFIMPGQALRKKSVRQKLNAIAEEFKGKNVLLVDDSIVRGTTSREIVQMARDAGAEKVYFASASPPV
RFCNVYGIDMPSRRELIANGRNDEEIAREIGCDALIYQDLDAMVEVVRAANPAIREFDTSCFNGCYITGDITPEYLDYVE
AARDGESPPPSALSTQQLDLNLATGS

Sequences:

>Translated_506_residues
MCGVIGVVANTAVNQLLYDGLMVLQHRGQDAAGIVTAEENRFHMHKAPGLARDVFRTRDMRNLLGDMGVAHVRYPTAGSA
SSSAESQPFYVNSPYGIVLGHNGNLTNTHELKESLFRSDLRHVNTNSDSEVLLNVLAHELHVRASGQQLSLDTIFGAVAG
VHARCKGAYAVTAFIAGYGLLAFRDPHGIRPLVIGINDQVTPHEYIVASESVAIDTLGFRLLRDVAPGEAVFIDYERRLH
SRQCSDNPVLNPCIFEYVYLARPDSVMDGISVYSTRLAMGVSLAEKIRREFGHLQIDVVIPIPDTSRPSALQLANHLAVP
YREGFIKNRYIGRTFIMPGQALRKKSVRQKLNAIAEEFKGKNVLLVDDSIVRGTTSREIVQMARDAGAEKVYFASASPPV
RFCNVYGIDMPSRRELIANGRNDEEIAREIGCDALIYQDLDAMVEVVRAANPAIREFDTSCFNGCYITGDITPEYLDYVE
AARDGESPPPSALSTQQLDLNLATGS
>Mature_506_residues
MCGVIGVVANTAVNQLLYDGLMVLQHRGQDAAGIVTAEENRFHMHKAPGLARDVFRTRDMRNLLGDMGVAHVRYPTAGSA
SSSAESQPFYVNSPYGIVLGHNGNLTNTHELKESLFRSDLRHVNTNSDSEVLLNVLAHELHVRASGQQLSLDTIFGAVAG
VHARCKGAYAVTAFIAGYGLLAFRDPHGIRPLVIGINDQVTPHEYIVASESVAIDTLGFRLLRDVAPGEAVFIDYERRLH
SRQCSDNPVLNPCIFEYVYLARPDSVMDGISVYSTRLAMGVSLAEKIRREFGHLQIDVVIPIPDTSRPSALQLANHLAVP
YREGFIKNRYIGRTFIMPGQALRKKSVRQKLNAIAEEFKGKNVLLVDDSIVRGTTSREIVQMARDAGAEKVYFASASPPV
RFCNVYGIDMPSRRELIANGRNDEEIAREIGCDALIYQDLDAMVEVVRAANPAIREFDTSCFNGCYITGDITPEYLDYVE
AARDGESPPPSALSTQQLDLNLATGS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=476, Percent_Identity=34.8739495798319, Blast_Score=259, Evalue=5e-69,
Organism=Escherichia coli, GI1788651, Length=489, Percent_Identity=60.5316973415133, Blast_Score=602, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI17554892, Length=489, Percent_Identity=32.5153374233129, Blast_Score=232, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6323958, Length=499, Percent_Identity=50.1002004008016, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24659598, Length=512, Percent_Identity=34.1796875, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24659604, Length=488, Percent_Identity=34.8360655737705, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI28573187, Length=469, Percent_Identity=34.3283582089552, Blast_Score=253, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55578; Mature: 55578

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVIGVVANTAVNQLLYDGLMVLQHRGQDAAGIVTAEENRFHMHKAPGLARDVFRTRDM
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHH
RNLLGDMGVAHVRYPTAGSASSSAESQPFYVNSPYGIVLGHNGNLTNTHELKESLFRSDL
HHHHHHCCCEEEECCCCCCCCCCCCCCCEEEECCEEEEEECCCCCCCHHHHHHHHHHHHH
RHVNTNSDSEVLLNVLAHELHVRASGQQLSLDTIFGAVAGVHARCKGAYAVTAFIAGYGL
HHCCCCCHHHHHHHHHHHHHHEECCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHCCE
LAFRDPHGIRPLVIGINDQVTPHEYIVASESVAIDTLGFRLLRDVAPGEAVFIDYERRLH
EEEECCCCCCEEEEECCCCCCCCCEEEECCCEEHHHHHHHHHHHCCCCCEEEEEHHHHHH
SRQCSDNPVLNPCIFEYVYLARPDSVMDGISVYSTRLAMGVSLAEKIRREFGHLQIDVVI
HCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
PIPDTSRPSALQLANHLAVPYREGFIKNRYIGRTFIMPGQALRKKSVRQKLNAIAEEFKG
ECCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCC
KNVLLVDDSIVRGTTSREIVQMARDAGAEKVYFASASPPVRFCNVYGIDMPSRRELIANG
CEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHCC
RNDEEIAREIGCDALIYQDLDAMVEVVRAANPAIREFDTSCFNGCYITGDITPEYLDYVE
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHCHHHHCCEEEECCCCHHHHHHHH
AARDGESPPPSALSTQQLDLNLATGS
HHHCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MCGVIGVVANTAVNQLLYDGLMVLQHRGQDAAGIVTAEENRFHMHKAPGLARDVFRTRDM
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHH
RNLLGDMGVAHVRYPTAGSASSSAESQPFYVNSPYGIVLGHNGNLTNTHELKESLFRSDL
HHHHHHCCCEEEECCCCCCCCCCCCCCCEEEECCEEEEEECCCCCCCHHHHHHHHHHHHH
RHVNTNSDSEVLLNVLAHELHVRASGQQLSLDTIFGAVAGVHARCKGAYAVTAFIAGYGL
HHCCCCCHHHHHHHHHHHHHHEECCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHCCE
LAFRDPHGIRPLVIGINDQVTPHEYIVASESVAIDTLGFRLLRDVAPGEAVFIDYERRLH
EEEECCCCCCEEEEECCCCCCCCCEEEECCCEEHHHHHHHHHHHCCCCCEEEEEHHHHHH
SRQCSDNPVLNPCIFEYVYLARPDSVMDGISVYSTRLAMGVSLAEKIRREFGHLQIDVVI
HCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
PIPDTSRPSALQLANHLAVPYREGFIKNRYIGRTFIMPGQALRKKSVRQKLNAIAEEFKG
ECCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCC
KNVLLVDDSIVRGTTSREIVQMARDAGAEKVYFASASPPVRFCNVYGIDMPSRRELIANG
CEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHCC
RNDEEIAREIGCDALIYQDLDAMVEVVRAANPAIREFDTSCFNGCYITGDITPEYLDYVE
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHCHHHHCCEEEECCCCHHHHHHHH
AARDGESPPPSALSTQQLDLNLATGS
HHHCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]