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The map label for this gene is metZ [H]

Identifier: 74317925

GI number: 74317925

Start: 2002799

End: 2003968

Strand: Reverse

Name: metZ [H]

Synonym: Tbd_1907

Alternate gene names: 74317925

Gene position: 2003968-2002799 (Counterclockwise)

Preceding gene: 74317926

Following gene: 74317924

Centisome position: 68.87

GC content: 67.35

Gene sequence:

>1170_bases
ATGAACGACGCATACGACATCGAAACCCTTGGGGTGCGCGCCGGCTTGGTCCGCAGCCAGTTCAACGAGCATTCCGAGGC
CATGTTCCTGACCTCGAGCTTCGTTTTCGGCAGCGCTGCCGAAGCCGCCGCGCGCTTCAAGGGTGACGAGCCGGGACCGA
TCTACGCCCGTTTCACCAACCCGACCGTGCAGATGATGGAGGCGCGGCTCGCGGCGCTCGAGGGGGCCGAGCGCTGCGTC
GCCTTCGCCTCGGGCATGGCGGCGATCCTCGCGACCGTGATGGGGCTCATGAAGGGCGGCGAGCACGTCGTCGCCTCGCG
CTCGATCTTCGGCTCGACCGTGCAGCTGTTCTCGAACATCCTCGGCCGCTTCGGCATCGAAACGAGCTATGTCTCGCCGA
CCGACCCGGCCGAATGGCGCGCAGCGGTGAAACCGAACACCAGGCTTTTCTTCGTCGAATCGCCGTCCAATCCCCTGACC
GAGGTCAGCGACATCCGCGCGCTCGCCGCGATCGCCCGCGAGGCGGGCGCCTGGCTCGCGGTCGACAACTGCTTCTGCTC
GCCGGCGCTGCAAAAGCCGCTCGAGCTCGGCGCCGACATCGTGATCCATTCGGCGACCAAGTATCTGGACGGGCAGGGGA
GGGTGCTCGGCGGCGCCGTGCTCGGCAGCCAGGCGCTGATGGAAGGTGTCTACACCTTCCTGCGCACGGCCGGTCCGACG
CTGTCGGCCTTCAACGCCTGGGTGATCCTCAAGGGCATGGAGACGCTGGCGCTGCGCATGGACGCCCATTCGAAGAACGC
GCTGGCGCTGGCGCAGTGGCTGGAGGCGCAGCCGCAGGTCGTGCGCGTGCTCTACCCGGGTCTGCCGTCGCATCCGCAGC
ACGAACTGGCGATGACGCAGCAGAAAACCGGCGGCGGCATCGTCGCCTTCGAACTGAAGGGCGGAAAGGACGCAGCGTGG
AAGCTGATCGACGCGACGAAGCTCTTGTCGATCACCGCCAACCTCGGCGACACCAAGACCACGATCACGCATCCGGCGAG
CACCACGCACAGCCGCATGACGCCCGAGCAGCGGCTGGCGGCCGGGATCGGCGACGGTCTTGTACGCATCGCAGTGGGTC
TCGAGTCGGTCGCGGACATCGAAGCCGATCTGGCGCGAGGGCTGGCGTGA

Upstream 100 bases:

>100_bases
GAATCCCAGCTTGCGGGCGCTTGAAAAATACGGCTAAAGCGAGGCGACGGAAACCCCGCGGCGCGCTTGGCGTTCGGGGT
TTTTTCTTTTGGACTCGAAG

Downstream 100 bases:

>100_bases
GGCGCTGGCTCGCTCTGCTCTGTCTTGCCGCGACGGGTACGCTTCACGCGAGCGAGCCGACGGACGCGGGGGCGACGATG
ACCGCGCTGCGCTACGTCGA

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNDAYDIETLGVRAGLVRSQFNEHSEAMFLTSSFVFGSAAEAAARFKGDEPGPIYARFTNPTVQMMEARLAALEGAERCV
AFASGMAAILATVMGLMKGGEHVVASRSIFGSTVQLFSNILGRFGIETSYVSPTDPAEWRAAVKPNTRLFFVESPSNPLT
EVSDIRALAAIAREAGAWLAVDNCFCSPALQKPLELGADIVIHSATKYLDGQGRVLGGAVLGSQALMEGVYTFLRTAGPT
LSAFNAWVILKGMETLALRMDAHSKNALALAQWLEAQPQVVRVLYPGLPSHPQHELAMTQQKTGGGIVAFELKGGKDAAW
KLIDATKLLSITANLGDTKTTITHPASTTHSRMTPEQRLAAGIGDGLVRIAVGLESVADIEADLARGLA

Sequences:

>Translated_389_residues
MNDAYDIETLGVRAGLVRSQFNEHSEAMFLTSSFVFGSAAEAAARFKGDEPGPIYARFTNPTVQMMEARLAALEGAERCV
AFASGMAAILATVMGLMKGGEHVVASRSIFGSTVQLFSNILGRFGIETSYVSPTDPAEWRAAVKPNTRLFFVESPSNPLT
EVSDIRALAAIAREAGAWLAVDNCFCSPALQKPLELGADIVIHSATKYLDGQGRVLGGAVLGSQALMEGVYTFLRTAGPT
LSAFNAWVILKGMETLALRMDAHSKNALALAQWLEAQPQVVRVLYPGLPSHPQHELAMTQQKTGGGIVAFELKGGKDAAW
KLIDATKLLSITANLGDTKTTITHPASTTHSRMTPEQRLAAGIGDGLVRIAVGLESVADIEADLARGLA
>Mature_389_residues
MNDAYDIETLGVRAGLVRSQFNEHSEAMFLTSSFVFGSAAEAAARFKGDEPGPIYARFTNPTVQMMEARLAALEGAERCV
AFASGMAAILATVMGLMKGGEHVVASRSIFGSTVQLFSNILGRFGIETSYVSPTDPAEWRAAVKPNTRLFFVESPSNPLT
EVSDIRALAAIAREAGAWLAVDNCFCSPALQKPLELGADIVIHSATKYLDGQGRVLGGAVLGSQALMEGVYTFLRTAGPT
LSAFNAWVILKGMETLALRMDAHSKNALALAQWLEAQPQVVRVLYPGLPSHPQHELAMTQQKTGGGIVAFELKGGKDAAW
KLIDATKLLSITANLGDTKTTITHPASTTHSRMTPEQRLAAGIGDGLVRIAVGLESVADIEADLARGLA

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=38.4146341463415, Blast_Score=235, Evalue=6e-62,
Organism=Homo sapiens, GI299473759, Length=328, Percent_Identity=34.7560975609756, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI25453487, Length=327, Percent_Identity=32.7217125382263, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1790375, Length=382, Percent_Identity=36.3874345549738, Blast_Score=237, Evalue=8e-64,
Organism=Escherichia coli, GI1789383, Length=363, Percent_Identity=28.3746556473829, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17538055, Length=341, Percent_Identity=39.0029325513196, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17560056, Length=338, Percent_Identity=39.0532544378698, Blast_Score=250, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI71980708, Length=368, Percent_Identity=33.4239130434783, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319307, Length=370, Percent_Identity=35.6756756756757, Blast_Score=222, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=32.9466357308585, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6321254, Length=408, Percent_Identity=25.9803921568627, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6321170, Length=291, Percent_Identity=30.5841924398625, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321904, Length=348, Percent_Identity=29.0229885057471, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922554, Length=350, Percent_Identity=37.1428571428571, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 41316; Mature: 41316

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAYDIETLGVRAGLVRSQFNEHSEAMFLTSSFVFGSAAEAAARFKGDEPGPIYARFTN
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHHCCCCCCCCEEEEECC
PTVQMMEARLAALEGAERCVAFASGMAAILATVMGLMKGGEHVVASRSIFGSTVQLFSNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
LGRFGIETSYVSPTDPAEWRAAVKPNTRLFFVESPSNPLTEVSDIRALAAIAREAGAWLA
HHHCCCCCCCCCCCCCHHCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEE
VDNCFCSPALQKPLELGADIVIHSATKYLDGQGRVLGGAVLGSQALMEGVYTFLRTAGPT
ECCCCCCHHHHHHHHCCCCEEEECHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
LSAFNAWVILKGMETLALRMDAHSKNALALAQWLEAQPQVVRVLYPGLPSHPQHELAMTQ
HHHHCCEEEEECHHHEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHH
QKTGGGIVAFELKGGKDAAWKLIDATKLLSITANLGDTKTTITHPASTTHSRMTPEQRLA
HCCCCCEEEEEECCCCCHHHHHHHHHEEEEEEECCCCCCEEEECCCCCCHHCCCHHHHHH
AGIGDGLVRIAVGLESVADIEADLARGLA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDAYDIETLGVRAGLVRSQFNEHSEAMFLTSSFVFGSAAEAAARFKGDEPGPIYARFTN
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHHCCCCCCCCEEEEECC
PTVQMMEARLAALEGAERCVAFASGMAAILATVMGLMKGGEHVVASRSIFGSTVQLFSNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
LGRFGIETSYVSPTDPAEWRAAVKPNTRLFFVESPSNPLTEVSDIRALAAIAREAGAWLA
HHHCCCCCCCCCCCCCHHCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEE
VDNCFCSPALQKPLELGADIVIHSATKYLDGQGRVLGGAVLGSQALMEGVYTFLRTAGPT
ECCCCCCHHHHHHHHCCCCEEEECHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
LSAFNAWVILKGMETLALRMDAHSKNALALAQWLEAQPQVVRVLYPGLPSHPQHELAMTQ
HHHHCCEEEEECHHHEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHH
QKTGGGIVAFELKGGKDAAWKLIDATKLLSITANLGDTKTTITHPASTTHSRMTPEQRLA
HCCCCCEEEEEECCCCCHHHHHHHHHEEEEEEECCCCCCEEEECCCCCCHHCCCHHHHHH
AGIGDGLVRIAVGLESVADIEADLARGLA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]