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The map label for this gene is cysS

Identifier: 74317920

GI number: 74317920

Start: 1998265

End: 1999629

Strand: Reverse

Name: cysS

Synonym: Tbd_1902

Alternate gene names: 74317920

Gene position: 1999629-1998265 (Counterclockwise)

Preceding gene: 74317924

Following gene: 74317919

Centisome position: 68.72

GC content: 67.33

Gene sequence:

>1365_bases
ATGCTGCACATCACCAACTCCCTCACGCGTACCAAGGAAGAATTCGTCCCGCTCAGCCCCGGCCGCGTGCGTATGTACGT
CTGCGGCATGACCGTGTACGACCTCTGCCATCTCGGGCACGCACGCGTGTTCGTCGTGTTCGACATGGTGACCCGCTGGC
TGCGCGCGAGCGGCTACGCGGTCGAGTACGTGCGCAACATCACCGACATCGACGACAAGATCATCAAGCGCGCGCACGAG
AACAAGGAAACCCCGGCCGCGCTGACCGAGCGCTTCATCGCGGAAATGCACGAGGACGAGCGTGCGCTGGGCGTGCTGCC
GCCCGACCATGAGCCACGGGCCACCGCCTACGTCGCGCAGATGCTTGCGCTGATCGCGCAACTGGTCGACAGGGGTCTCG
CCTATGCGGCGCCCAACGCCGACGTCTACTACAGCGTGCGCGGCTTTCCCGCCTACGGCCGGCTGTCGGGCAAGAGCCTC
GACGAATTGCGCGCCGGCGAGCGCGTCGAGGTCGATCCGAACAAGCGCGATCCGATGGATTTCGTGCTGTGGAAGGCCGC
CAAGCCCGGCGAGCCGGCCTGGGAGTCGCCGTGGGGGCAGGGCCGCCCCGGCTGGCACATCGAGTGTTCGGCGATGAGCG
CTGAACTCCTCGGCCAGCACTTCGACATCCACGGCGGCGGCCAGGATCTGCAGTTCCCACACCACGAGAACGAGATCGCG
CAGTCCGAGGGCGCGCACGGCTGCACCTTCGTCAACTACTGGCTGCACAACGGCTTCGTCCGCGTCGACAACGAAAAGAT
GTCGAAGTCGCTCGGCAATTTCTTCACGATCCGCGAGGTGCTGCAGAAATACGACGCCGAGATCGTGCGCTTCTTCATCC
TGCGCGCGCATTACCGCAGCCCGCTCAATTATTCCGATGCCCATCTCGACGACGCACGCGGCGCGCTGACCCGGCTCTAC
ACCGCGTTGAAAGGCGCGCCGGCCGCCGGCCCCGTCGATTGGGATGCGCCCGCCGCGGCGCGCTTCCGCGGGGCGATGGA
CGACGATTTCAATACGCCCGAAGCGATCGCGGTCCTTTTCGAACTCGCGGCAGAGGCCAACCGCGGCGACACCGGCGCGG
CGCAGACCTTGCGTAACCTCGGCGCGGTGATCGGGCTACTGCAACGCGACGCGAGCGATTTCCTGCAGGCGGGTACCTCG
GAGGCGGGACTCGATGCCAGCGAGATCGAAGCCAGGATCGCCGCGCGCCAGGCCGCGCGCAAGGCGCGCGACTTCGCCGA
GTCGGATCGCATCCGCGACGAGCTCGCGGCCGCCGGCATCGTGCTCGAGGACGGCCCCGGCGGCACGACCTGGCGGCGCC
AGTAA

Upstream 100 bases:

>100_bases
CATCTCGGGAAACCGGCATTCTAGCAGCGTCGAACGGGCTCAGACACCGCTTCGACGCGTGTTTCCAGCCTGTCCTCAAC
GCTTCCGTCCGATTCGCCCC

Downstream 100 bases:

>100_bases
GCGCGCGAACCGTGGAATCGAGCGCGCTCGTTCTGGTCGGGGCCGTCGACTGGGTGCATCCGGCGTGGCGCGGGCAGTTT
TATCCCGAGGGCCTGCCGGA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MLHITNSLTRTKEEFVPLSPGRVRMYVCGMTVYDLCHLGHARVFVVFDMVTRWLRASGYAVEYVRNITDIDDKIIKRAHE
NKETPAALTERFIAEMHEDERALGVLPPDHEPRATAYVAQMLALIAQLVDRGLAYAAPNADVYYSVRGFPAYGRLSGKSL
DELRAGERVEVDPNKRDPMDFVLWKAAKPGEPAWESPWGQGRPGWHIECSAMSAELLGQHFDIHGGGQDLQFPHHENEIA
QSEGAHGCTFVNYWLHNGFVRVDNEKMSKSLGNFFTIREVLQKYDAEIVRFFILRAHYRSPLNYSDAHLDDARGALTRLY
TALKGAPAAGPVDWDAPAAARFRGAMDDDFNTPEAIAVLFELAAEANRGDTGAAQTLRNLGAVIGLLQRDASDFLQAGTS
EAGLDASEIEARIAARQAARKARDFAESDRIRDELAAAGIVLEDGPGGTTWRRQ

Sequences:

>Translated_454_residues
MLHITNSLTRTKEEFVPLSPGRVRMYVCGMTVYDLCHLGHARVFVVFDMVTRWLRASGYAVEYVRNITDIDDKIIKRAHE
NKETPAALTERFIAEMHEDERALGVLPPDHEPRATAYVAQMLALIAQLVDRGLAYAAPNADVYYSVRGFPAYGRLSGKSL
DELRAGERVEVDPNKRDPMDFVLWKAAKPGEPAWESPWGQGRPGWHIECSAMSAELLGQHFDIHGGGQDLQFPHHENEIA
QSEGAHGCTFVNYWLHNGFVRVDNEKMSKSLGNFFTIREVLQKYDAEIVRFFILRAHYRSPLNYSDAHLDDARGALTRLY
TALKGAPAAGPVDWDAPAAARFRGAMDDDFNTPEAIAVLFELAAEANRGDTGAAQTLRNLGAVIGLLQRDASDFLQAGTS
EAGLDASEIEARIAARQAARKARDFAESDRIRDELAAAGIVLEDGPGGTTWRRQ
>Mature_454_residues
MLHITNSLTRTKEEFVPLSPGRVRMYVCGMTVYDLCHLGHARVFVVFDMVTRWLRASGYAVEYVRNITDIDDKIIKRAHE
NKETPAALTERFIAEMHEDERALGVLPPDHEPRATAYVAQMLALIAQLVDRGLAYAAPNADVYYSVRGFPAYGRLSGKSL
DELRAGERVEVDPNKRDPMDFVLWKAAKPGEPAWESPWGQGRPGWHIECSAMSAELLGQHFDIHGGGQDLQFPHHENEIA
QSEGAHGCTFVNYWLHNGFVRVDNEKMSKSLGNFFTIREVLQKYDAEIVRFFILRAHYRSPLNYSDAHLDDARGALTRLY
TALKGAPAAGPVDWDAPAAARFRGAMDDDFNTPEAIAVLFELAAEANRGDTGAAQTLRNLGAVIGLLQRDASDFLQAGTS
EAGLDASEIEARIAARQAARKARDFAESDRIRDELAAAGIVLEDGPGGTTWRRQ

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=391, Percent_Identity=40.6649616368286, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI303304987, Length=233, Percent_Identity=43.7768240343348, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI21269877, Length=233, Percent_Identity=43.7768240343348, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI10835051, Length=233, Percent_Identity=43.7768240343348, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI62240992, Length=233, Percent_Identity=43.7768240343348, Blast_Score=199, Evalue=4e-51,
Organism=Escherichia coli, GI1786737, Length=466, Percent_Identity=59.656652360515, Blast_Score=538, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17509797, Length=244, Percent_Identity=40.5737704918033, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17509795, Length=244, Percent_Identity=40.5737704918033, Blast_Score=184, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324082, Length=364, Percent_Identity=32.4175824175824, Blast_Score=200, Evalue=4e-52,
Organism=Drosophila melanogaster, GI19922218, Length=504, Percent_Identity=33.9285714285714, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI19922364, Length=232, Percent_Identity=45.6896551724138, Blast_Score=204, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_THIDA (Q3SHN1)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315660.1
- ProteinModelPortal:   Q3SHN1
- SMR:   Q3SHN1
- STRING:   Q3SHN1
- GeneID:   3674012
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1902
- NMPDR:   fig|292415.3.peg.1999
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   Q3SHN1
- BioCyc:   TDEN292415:TBD_1902-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 50393; Mature: 50393

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHITNSLTRTKEEFVPLSPGRVRMYVCGMTVYDLCHLGHARVFVVFDMVTRWLRASGYA
CEECCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHH
VEYVRNITDIDDKIIKRAHENKETPAALTERFIAEMHEDERALGVLPPDHEPRATAYVAQ
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHH
MLALIAQLVDRGLAYAAPNADVYYSVRGFPAYGRLSGKSLDELRAGERVEVDPNKRDPMD
HHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCCCCCHHHHHCCCEEECCCCCCCCHH
FVLWKAAKPGEPAWESPWGQGRPGWHIECSAMSAELLGQHFDIHGGGQDLQFPHHENEIA
HEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
QSEGAHGCTFVNYWLHNGFVRVDNEKMSKSLGNFFTIREVLQKYDAEIVRFFILRAHYRS
HCCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLNYSDAHLDDARGALTRLYTALKGAPAAGPVDWDAPAAARFRGAMDDDFNTPEAIAVLF
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHH
ELAAEANRGDTGAAQTLRNLGAVIGLLQRDASDFLQAGTSEAGLDASEIEARIAARQAAR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KARDFAESDRIRDELAAAGIVLEDGPGGTTWRRQ
HHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MLHITNSLTRTKEEFVPLSPGRVRMYVCGMTVYDLCHLGHARVFVVFDMVTRWLRASGYA
CEECCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHH
VEYVRNITDIDDKIIKRAHENKETPAALTERFIAEMHEDERALGVLPPDHEPRATAYVAQ
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHH
MLALIAQLVDRGLAYAAPNADVYYSVRGFPAYGRLSGKSLDELRAGERVEVDPNKRDPMD
HHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCCCCCHHHHHCCCEEECCCCCCCCHH
FVLWKAAKPGEPAWESPWGQGRPGWHIECSAMSAELLGQHFDIHGGGQDLQFPHHENEIA
HEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
QSEGAHGCTFVNYWLHNGFVRVDNEKMSKSLGNFFTIREVLQKYDAEIVRFFILRAHYRS
HCCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLNYSDAHLDDARGALTRLYTALKGAPAAGPVDWDAPAAARFRGAMDDDFNTPEAIAVLF
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHH
ELAAEANRGDTGAAQTLRNLGAVIGLLQRDASDFLQAGTSEAGLDASEIEARIAARQAAR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KARDFAESDRIRDELAAAGIVLEDGPGGTTWRRQ
HHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA