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The map label for this gene is rfbH [H]

Identifier: 74317897

GI number: 74317897

Start: 1976102

End: 1977424

Strand: Reverse

Name: rfbH [H]

Synonym: Tbd_1879

Alternate gene names: 74317897

Gene position: 1977424-1976102 (Counterclockwise)

Preceding gene: 74317898

Following gene: 74317896

Centisome position: 67.96

GC content: 54.12

Gene sequence:

>1323_bases
ATGGAATCCTCTTCCGCAATGTTAAAGGAACAAATCCTTTCTCTCGTAAACCAGTACGCCGACCAGGTAATGTCTTCTAA
GCCGTTCACGGCGGGTCAATCGGTCATCCCACCGTCTGGCAAGGTAATAGGCGCATTGGAGCTCCAATACATGACCGAGG
CGGCTCTCGATGGTTGGCTCACGACCGGCCGTTTTAACGAAGCGTTCGAGTTAGAGCTTGCGAAATTTCTCGGCGTCAAA
CACGTCCTCACGACGAACTCCGGGTCGTCAGCCAATCTGCTGGCACTGGCTGCGCTGACGTCACCCAAGCTGGGAGATAG
AGCATTGCGGCCTGGCGACGAGGTCATTACGGTGGCCGCAGGGTTTCCGACGACCGTCAACCCGAGCATCCTGTTCGGTC
TAATACCGGTGTTCGTGGATGTGCATATCCCAACATACAACATTGATCCCGCCAAGATCGAAGCCGCGATCAGTCCCAAA
ACCCGCGCCATCATGATTGCTCACACTCTGGGCAATCCGTTCGACATCGATGCAGTTACGCGACTGGCTAAGCAGCATAA
TCTCTGGCTCATCGAGGACTGCTGCGATGCGCTGGGTTCTACCTATGACGGACGCAAGGTCGGCACCTTCGGCGACATTG
GAACTCTGAGTTTCTACCCTGCCCATCACATCACAATGGGAGAAGGCGGCGCAGTATTCACCAACGATGGACTACTCAAG
ACCATTATCGAGTCATTCCGCGATTGGGGTCGGGACTGCTACTGTGCGCCCGGCAAGGACAATACCTGCGGCAAGCGTTT
TGGCTGGAAGTTGGGCACGCTCCCTTTTGGCTACGATCACAAATACACGTACTCGCACCTCGGGTTCAACTTGAAGATCA
CCGACATGCAGGCGGCATGCGGGCTCGCTCAGCTACAGCAGGTGGATCATTTCATTGAGACGAGAAAGCGAAATTTCAAC
TATCTGCGGGACCGATTGAAAAGATCGTGCCAAGAATTCCTCATCCTGCCTGAGGCGACACCCAAATCGGATCCGTCGTG
GTTTGGGTTTCCGATTACCTTGCGAGACGACGTCGAGTTCGATCGAGTCGCGCTCCTCCAGTATTTGGACGAGAGAAAAG
TTGGCACGCGACTGCTTTTTGCGGGAAACCTCACACGCCAGCCCTACATGACTGGCCGGAATTATCGCGTCGCAGGCACT
TTGAACAATACGGATATCGTGATGAATAGTACTTTCTGGGTCGGTGTGTACCCCGGCTTGACCGAAGAGATGCTGGAGTA
TATGGCGGTCCAGATTGAGTTGTTCCTCGGAATTGGGTTCTAA

Upstream 100 bases:

>100_bases
CGAGTGGTACCGGGCAGCCGGATCGGGCGCTGACATGCGCCCGTTCACGCTTGGCCAAATCGAAGCCTACCTCTGATAAA
ACGTTATAGAAAGTCATGAA

Downstream 100 bases:

>100_bases
AAAAGAAGCGGGGGACGCCCCCTCGTTGAGACATGACAGACTACTTCAGTATCAGTTTTACCAGACGCTTACAGGACGCC
ATCTCAGACATGGCCAAAAG

Product: putative dehydratase RfbH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MESSSAMLKEQILSLVNQYADQVMSSKPFTAGQSVIPPSGKVIGALELQYMTEAALDGWLTTGRFNEAFELELAKFLGVK
HVLTTNSGSSANLLALAALTSPKLGDRALRPGDEVITVAAGFPTTVNPSILFGLIPVFVDVHIPTYNIDPAKIEAAISPK
TRAIMIAHTLGNPFDIDAVTRLAKQHNLWLIEDCCDALGSTYDGRKVGTFGDIGTLSFYPAHHITMGEGGAVFTNDGLLK
TIIESFRDWGRDCYCAPGKDNTCGKRFGWKLGTLPFGYDHKYTYSHLGFNLKITDMQAACGLAQLQQVDHFIETRKRNFN
YLRDRLKRSCQEFLILPEATPKSDPSWFGFPITLRDDVEFDRVALLQYLDERKVGTRLLFAGNLTRQPYMTGRNYRVAGT
LNNTDIVMNSTFWVGVYPGLTEEMLEYMAVQIELFLGIGF

Sequences:

>Translated_440_residues
MESSSAMLKEQILSLVNQYADQVMSSKPFTAGQSVIPPSGKVIGALELQYMTEAALDGWLTTGRFNEAFELELAKFLGVK
HVLTTNSGSSANLLALAALTSPKLGDRALRPGDEVITVAAGFPTTVNPSILFGLIPVFVDVHIPTYNIDPAKIEAAISPK
TRAIMIAHTLGNPFDIDAVTRLAKQHNLWLIEDCCDALGSTYDGRKVGTFGDIGTLSFYPAHHITMGEGGAVFTNDGLLK
TIIESFRDWGRDCYCAPGKDNTCGKRFGWKLGTLPFGYDHKYTYSHLGFNLKITDMQAACGLAQLQQVDHFIETRKRNFN
YLRDRLKRSCQEFLILPEATPKSDPSWFGFPITLRDDVEFDRVALLQYLDERKVGTRLLFAGNLTRQPYMTGRNYRVAGT
LNNTDIVMNSTFWVGVYPGLTEEMLEYMAVQIELFLGIGF
>Mature_440_residues
MESSSAMLKEQILSLVNQYADQVMSSKPFTAGQSVIPPSGKVIGALELQYMTEAALDGWLTTGRFNEAFELELAKFLGVK
HVLTTNSGSSANLLALAALTSPKLGDRALRPGDEVITVAAGFPTTVNPSILFGLIPVFVDVHIPTYNIDPAKIEAAISPK
TRAIMIAHTLGNPFDIDAVTRLAKQHNLWLIEDCCDALGSTYDGRKVGTFGDIGTLSFYPAHHITMGEGGAVFTNDGLLK
TIIESFRDWGRDCYCAPGKDNTCGKRFGWKLGTLPFGYDHKYTYSHLGFNLKITDMQAACGLAQLQQVDHFIETRKRNFN
YLRDRLKRSCQEFLILPEATPKSDPSWFGFPITLRDDVEFDRVALLQYLDERKVGTRLLFAGNLTRQPYMTGRNYRVAGT
LNNTDIVMNSTFWVGVYPGLTEEMLEYMAVQIELFLGIGF

Specific function: Unknown

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI145693159, Length=406, Percent_Identity=29.5566502463054, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI2367285, Length=343, Percent_Identity=34.402332361516, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 48787; Mature: 48787

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSSAMLKEQILSLVNQYADQVMSSKPFTAGQSVIPPSGKVIGALELQYMTEAALDGWL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCE
TTGRFNEAFELELAKFLGVKHVLTTNSGSSANLLALAALTSPKLGDRALRPGDEVITVAA
ECCCCCCHHHHHHHHHHCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEE
GFPTTVNPSILFGLIPVFVDVHIPTYNIDPAKIEAAISPKTRAIMIAHTLGNPFDIDAVT
CCCCCCCHHHHHHHHHHHEEEECCCCCCCHHHEEEEECCCCEEEEEEECCCCCCCHHHHH
RLAKQHNLWLIEDCCDALGSTYDGRKVGTFGDIGTLSFYPAHHITMGEGGAVFTNDGLLK
HHHHHCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEECCCCEEEECCCHHH
TIIESFRDWGRDCYCAPGKDNTCGKRFGWKLGTLPFGYDHKYTYSHLGFNLKITDMQAAC
HHHHHHHHCCCCEEECCCCCCCCCHHCCCEECCCCCCCCCCEEHHHCCEEEEEECHHHHH
GLAQLQQVDHFIETRKRNFNYLRDRLKRSCQEFLILPEATPKSDPSWFGFPITLRDDVEF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEEEEECCCCH
DRVALLQYLDERKVGTRLLFAGNLTRQPYMTGRNYRVAGTLNNTDIVMNSTFWVGVYPGL
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECEEEEEECCCC
TEEMLEYMAVQIELFLGIGF
HHHHHHHHHHHEEEEEECCC
>Mature Secondary Structure
MESSSAMLKEQILSLVNQYADQVMSSKPFTAGQSVIPPSGKVIGALELQYMTEAALDGWL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCE
TTGRFNEAFELELAKFLGVKHVLTTNSGSSANLLALAALTSPKLGDRALRPGDEVITVAA
ECCCCCCHHHHHHHHHHCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEE
GFPTTVNPSILFGLIPVFVDVHIPTYNIDPAKIEAAISPKTRAIMIAHTLGNPFDIDAVT
CCCCCCCHHHHHHHHHHHEEEECCCCCCCHHHEEEEECCCCEEEEEEECCCCCCCHHHHH
RLAKQHNLWLIEDCCDALGSTYDGRKVGTFGDIGTLSFYPAHHITMGEGGAVFTNDGLLK
HHHHHCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEECCCCEEEECCCHHH
TIIESFRDWGRDCYCAPGKDNTCGKRFGWKLGTLPFGYDHKYTYSHLGFNLKITDMQAAC
HHHHHHHHCCCCEEECCCCCCCCCHHCCCEECCCCCCCCCCEEHHHCCEEEEEECHHHHH
GLAQLQQVDHFIETRKRNFNYLRDRLKRSCQEFLILPEATPKSDPSWFGFPITLRDDVEF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEEEEECCCCH
DRVALLQYLDERKVGTRLLFAGNLTRQPYMTGRNYRVAGTLNNTDIVMNSTFWVGVYPGL
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECEEEEEECCCC
TEEMLEYMAVQIELFLGIGF
HHHHHHHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]