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The map label for this gene is ilvB [H]

Identifier: 74317894

GI number: 74317894

Start: 1972718

End: 1974481

Strand: Reverse

Name: ilvB [H]

Synonym: Tbd_1876

Alternate gene names: 74317894

Gene position: 1974481-1972718 (Counterclockwise)

Preceding gene: 74317895

Following gene: 74317893

Centisome position: 67.86

GC content: 55.05

Gene sequence:

>1764_bases
ATGCGCTATAGCGATGTAATGGCCGATTGGCTTAAAGATATGGGTTTTACACACTGCTTCTTCGTAGCGGGAGGCAACAT
CATGCACATGGTGGCGAGCGCGAGTAAGCGCTTCACATGCATTCCCGTTGTTCATGAAGTCGCAGCAGGCATTGCAACTG
AGTACTTCAATGAAAGTAGCCGCACTGAGCGCGCGTTTGCTCTTGTGACTGCCGGGCCCGGGCTCACTAACATTGTTACG
GCGATAGGTGGTGCATGGCTCGAGAGTCGCGAGCTACTGGTCATTGGCGGGCAGGTTAAGACTGAGGACCTGAGCCGGAA
CACGCTGCGCCAGAAAGGCATACAAGAGATCGCGGGTGTGGATATCGTCCGGCCGATCACGCGTCACGCAATCCTGCTGG
ATGATGTTGCGGACCGCCAGACGTTCTCACGGTACGTGCAGGACGGGGCAGAAGGCCGGAAAGGTCCCGTGTTCATCGAG
ATTCCACTGGACGTCCAGGGACGCGAAGTTGACCCGGGGAACCTGAGCGGCCCTGAGTCGACCACCACCGAAGCGGCGCT
GCCGTGTCTGGATGAATCAGTTTTGCAAGGAATTGTCGAGGAGATCCGTGCTGCCAAACGTCCCATGCTGTTACTAGGTG
GTGGCGTGCACCGATCAATCACCGAAACACTCTACGACGGCCTTGCCGACATGGGTGTGCCGATCACCACCACCTGGAAC
GGCGCAGATCGCCTCGGCTTTGACCATCCTATGTATTTTGGCCGCCCTAATACGTGGGGGCAACGGTACAGCAACTTGTT
GTTGCAGCAATCCGATCTGATCGTCGCCTTGGGCACGCGCTTGGGACTGCAACAAACCGGATTTAACTGGAAGCAATTCG
CGCCGAAAGGGCGCGTGGTGCAGGTTGATTGTGATGCCGCAGAATTGACCAAGGGGCATCCGCGAGTCGACATTGCTTTG
CTCGCCGATGCAAATACCGTGCTGAAGTCTCTTTCAAACTATGAACTGGGAGACCATTCGGACTGGGGCGAATACTGCCG
TGTAGTGCGCGAGAAATTGCCGATCGTAGACCAGAGGAATACAACGAGAGAAGGCTATATCTGTCCCTACGTCTTCGTGC
AGCAGCTGTCACGCATGAGTGGTGCGAACGACGTCGTGATCCCGTGTAGCAGCGGTGGCGCTTTTACGGTAATGATGCAA
GCATTCGATCAGAAAAGAGGGCAGGTCATCATCACCGACAAGGGATTGGCCTCGATGGGCTATGGACTCAGCGGGGCCAT
TGGCGTGGCGTTCGCCCATCCGGATAGGCGGGTGATTCTTGTCGAGGGCGACGGAGGATTTGCCCAGAATTTGCAAGAGT
TGGGCACTGTGGCCATAAACAAACTGAATATGAAAATTTTCATTTTCGAAGACGGGGGATACGCCTCCATTCGTATGACT
CAGCGCAATTATTTCGGCGGACATTACGTCGGCTGCGACCGTTTGACCGGACTCGGACTTCCCAATTGGGACGCTCTTTT
TGCCGCCTACGGGATACCCTGTCTACGCATCGGGCCTGGTTTTGAGGAAAACGGGGATTTCTCTAACCTCTTTAACTCTT
TTGGTGTGGCAGCATTTTTGGTGGCGATTGACCCTGAACAGACCTATTTTCCAAAGATCACCAGTCGCGTGACCGACGCC
GGGAGTATGGAATCCAACCCACTTCACAGGATGACGCCAGATCTCGATGAAGAACTCTACGAGACGGTAGGCCGCTACCT
TTGA

Upstream 100 bases:

>100_bases
TTCGCTTGGAGCACGGCAGGCTTTCGGAGGGGGACCAGATGCTGGATCACCCGCAATAGCGCCGTGGGTGGATTGGGTTT
TTGATGAATTGGAGAAATGA

Downstream 100 bases:

>100_bases
GCCTCTGGACAAGTCGAAGTTGCGTGGCGAAGAGAGCGCTTCGATGGTATCGTTGCCGCCGATCTGCGTGGGGCGGCAAG
TCGATTTGGAGTAACGCGAC

Product: acetolactate synthase

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MRYSDVMADWLKDMGFTHCFFVAGGNIMHMVASASKRFTCIPVVHEVAAGIATEYFNESSRTERAFALVTAGPGLTNIVT
AIGGAWLESRELLVIGGQVKTEDLSRNTLRQKGIQEIAGVDIVRPITRHAILLDDVADRQTFSRYVQDGAEGRKGPVFIE
IPLDVQGREVDPGNLSGPESTTTEAALPCLDESVLQGIVEEIRAAKRPMLLLGGGVHRSITETLYDGLADMGVPITTTWN
GADRLGFDHPMYFGRPNTWGQRYSNLLLQQSDLIVALGTRLGLQQTGFNWKQFAPKGRVVQVDCDAAELTKGHPRVDIAL
LADANTVLKSLSNYELGDHSDWGEYCRVVREKLPIVDQRNTTREGYICPYVFVQQLSRMSGANDVVIPCSSGGAFTVMMQ
AFDQKRGQVIITDKGLASMGYGLSGAIGVAFAHPDRRVILVEGDGGFAQNLQELGTVAINKLNMKIFIFEDGGYASIRMT
QRNYFGGHYVGCDRLTGLGLPNWDALFAAYGIPCLRIGPGFEENGDFSNLFNSFGVAAFLVAIDPEQTYFPKITSRVTDA
GSMESNPLHRMTPDLDEELYETVGRYL

Sequences:

>Translated_587_residues
MRYSDVMADWLKDMGFTHCFFVAGGNIMHMVASASKRFTCIPVVHEVAAGIATEYFNESSRTERAFALVTAGPGLTNIVT
AIGGAWLESRELLVIGGQVKTEDLSRNTLRQKGIQEIAGVDIVRPITRHAILLDDVADRQTFSRYVQDGAEGRKGPVFIE
IPLDVQGREVDPGNLSGPESTTTEAALPCLDESVLQGIVEEIRAAKRPMLLLGGGVHRSITETLYDGLADMGVPITTTWN
GADRLGFDHPMYFGRPNTWGQRYSNLLLQQSDLIVALGTRLGLQQTGFNWKQFAPKGRVVQVDCDAAELTKGHPRVDIAL
LADANTVLKSLSNYELGDHSDWGEYCRVVREKLPIVDQRNTTREGYICPYVFVQQLSRMSGANDVVIPCSSGGAFTVMMQ
AFDQKRGQVIITDKGLASMGYGLSGAIGVAFAHPDRRVILVEGDGGFAQNLQELGTVAINKLNMKIFIFEDGGYASIRMT
QRNYFGGHYVGCDRLTGLGLPNWDALFAAYGIPCLRIGPGFEENGDFSNLFNSFGVAAFLVAIDPEQTYFPKITSRVTDA
GSMESNPLHRMTPDLDEELYETVGRYL
>Mature_587_residues
MRYSDVMADWLKDMGFTHCFFVAGGNIMHMVASASKRFTCIPVVHEVAAGIATEYFNESSRTERAFALVTAGPGLTNIVT
AIGGAWLESRELLVIGGQVKTEDLSRNTLRQKGIQEIAGVDIVRPITRHAILLDDVADRQTFSRYVQDGAEGRKGPVFIE
IPLDVQGREVDPGNLSGPESTTTEAALPCLDESVLQGIVEEIRAAKRPMLLLGGGVHRSITETLYDGLADMGVPITTTWN
GADRLGFDHPMYFGRPNTWGQRYSNLLLQQSDLIVALGTRLGLQQTGFNWKQFAPKGRVVQVDCDAAELTKGHPRVDIAL
LADANTVLKSLSNYELGDHSDWGEYCRVVREKLPIVDQRNTTREGYICPYVFVQQLSRMSGANDVVIPCSSGGAFTVMMQ
AFDQKRGQVIITDKGLASMGYGLSGAIGVAFAHPDRRVILVEGDGGFAQNLQELGTVAINKLNMKIFIFEDGGYASIRMT
QRNYFGGHYVGCDRLTGLGLPNWDALFAAYGIPCLRIGPGFEENGDFSNLFNSFGVAAFLVAIDPEQTYFPKITSRVTDA
GSMESNPLHRMTPDLDEELYETVGRYL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=537, Percent_Identity=24.3947858472998, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI93004078, Length=509, Percent_Identity=23.9685658153242, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=28.494623655914, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081685, Length=541, Percent_Identity=25.1386321626617, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1786717, Length=506, Percent_Identity=24.5059288537549, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1787096, Length=499, Percent_Identity=20.6412825651303, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17542570, Length=531, Percent_Identity=25.2354048964218, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17531299, Length=428, Percent_Identity=20.7943925233645, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17531301, Length=434, Percent_Identity=20.7373271889401, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323755, Length=543, Percent_Identity=25.7826887661142, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19922626, Length=492, Percent_Identity=23.3739837398374, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64337; Mature: 64337

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYSDVMADWLKDMGFTHCFFVAGGNIMHMVASASKRFTCIPVVHEVAAGIATEYFNESS
CCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCC
RTERAFALVTAGPGLTNIVTAIGGAWLESRELLVIGGQVKTEDLSRNTLRQKGIQEIAGV
CCCEEEEEEECCCCHHHHHHHHCCHHCCCCCEEEECCEEECCHHHHHHHHHHHHHHHHCC
DIVRPITRHAILLDDVADRQTFSRYVQDGAEGRKGPVFIEIPLDVQGREVDPGNLSGPES
HHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
TTTEAALPCLDESVLQGIVEEIRAAKRPMLLLGGGVHRSITETLYDGLADMGVPITTTWN
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEECC
GADRLGFDHPMYFGRPNTWGQRYSNLLLQQSDLIVALGTRLGLQQTGFNWKQFAPKGRVV
CCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHCCCCHHHCCCCCCEE
QVDCDAAELTKGHPRVDIALLADANTVLKSLSNYELGDHSDWGEYCRVVREKLPIVDQRN
EEECCHHHHCCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCC
TTREGYICPYVFVQQLSRMSGANDVVIPCSSGGAFTVMMQAFDQKRGQVIITDKGLASMG
CCCCCEECHHHHHHHHHHHCCCCCEEEECCCCCEEEHHHHHHHHCCCEEEEEECCHHHHC
YGLSGAIGVAFAHPDRRVILVEGDGGFAQNLQELGTVAINKLNMKIFIFEDGGYASIRMT
CCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHEEEEEEEEEEEECCCEEEEEEE
QRNYFGGHYVGCDRLTGLGLPNWDALFAAYGIPCLRIGPGFEENGDFSNLFNSFGVAAFL
CCCCCCCEEECHHHHCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCEEEE
VAIDPEQTYFPKITSRVTDAGSMESNPLHRMTPDLDEELYETVGRYL
EEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRYSDVMADWLKDMGFTHCFFVAGGNIMHMVASASKRFTCIPVVHEVAAGIATEYFNESS
CCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCC
RTERAFALVTAGPGLTNIVTAIGGAWLESRELLVIGGQVKTEDLSRNTLRQKGIQEIAGV
CCCEEEEEEECCCCHHHHHHHHCCHHCCCCCEEEECCEEECCHHHHHHHHHHHHHHHHCC
DIVRPITRHAILLDDVADRQTFSRYVQDGAEGRKGPVFIEIPLDVQGREVDPGNLSGPES
HHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
TTTEAALPCLDESVLQGIVEEIRAAKRPMLLLGGGVHRSITETLYDGLADMGVPITTTWN
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEECC
GADRLGFDHPMYFGRPNTWGQRYSNLLLQQSDLIVALGTRLGLQQTGFNWKQFAPKGRVV
CCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHCCCCHHHCCCCCCEE
QVDCDAAELTKGHPRVDIALLADANTVLKSLSNYELGDHSDWGEYCRVVREKLPIVDQRN
EEECCHHHHCCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCC
TTREGYICPYVFVQQLSRMSGANDVVIPCSSGGAFTVMMQAFDQKRGQVIITDKGLASMG
CCCCCEECHHHHHHHHHHHCCCCCEEEECCCCCEEEHHHHHHHHCCCEEEEEECCHHHHC
YGLSGAIGVAFAHPDRRVILVEGDGGFAQNLQELGTVAINKLNMKIFIFEDGGYASIRMT
CCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHEEEEEEEEEEEECCCEEEEEEE
QRNYFGGHYVGCDRLTGLGLPNWDALFAAYGIPCLRIGPGFEENGDFSNLFNSFGVAAFL
CCCCCCCEEECHHHHCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCEEEE
VAIDPEQTYFPKITSRVTDAGSMESNPLHRMTPDLDEELYETVGRYL
EEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]