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The map label for this gene is rpoE [H]

Identifier: 74317854

GI number: 74317854

Start: 1934951

End: 1935526

Strand: Reverse

Name: rpoE [H]

Synonym: Tbd_1836

Alternate gene names: 74317854

Gene position: 1935526-1934951 (Counterclockwise)

Preceding gene: 74317857

Following gene: 74317853

Centisome position: 66.52

GC content: 65.28

Gene sequence:

>576_bases
ATGGACCACCCGCGGCGTCTCCCTTCCATGAGCCTGATGAGCCACTTCCTATTCGGCCCCTTTCGACCCACTCTTGAAGC
CCTGCGGCCGATGTTGTACCGCATCGCCTACGCTTGGTGCCACGACCCCGCACTCGCCGACGATCTCGTCCAGGAGGCAT
TATCCAAGGCCTGGGCGCGCCGCGCCCAGCTCCGTGAGGACGCGGCGTTAAAGGGCTGGGTGGTCTCGATCATGAACCAT
TGCTGGGTCGACCATCTTCGCCGCCGGCGCGAGTTCGAGGACATCGAGGAATGGGAGGACGAACTCGAGTCGGCGGGCGA
CTCGCCCGAGGCATGCTGCGACCGCGAGCGCGTGGTCGCCTGTATCAGAAGTGCGGTCGCGCAGCTGCCGCCTGCGCAGC
GCCAAGTGGTCACGCTCGTCGACCTTGAGGAATTCGGCTATGCCGAGGTGGCGGGCATCCTGAACATTCCAGTCGGCACC
GTGATGAGCCGCCTGTCGCGGGCCCGTGCCAACCTGAAGAAACGGCTCGACGCGGCACAAACCGCGGCCGGGCCGTCATT
GTGGAGGGTGAAATGA

Upstream 100 bases:

>100_bases
TCTCCTTTTGGTTTTGATATCGGCTTTATCCAAGCCTAGTAGGAAGACGCCTCGACACGCACGCTTATTCCATAGCCGAC
GCGGTTTTTTTCTGGAATAA

Downstream 100 bases:

>100_bases
AATCGGTAGTGTCCGACGAAATCCTGCAGGCTTTTGTCGACGGGGAACTCAGTGTTCCCGAGAGCGAGGCGCTCGTTGCG
CGCATGCGCGAGGACAGCGA

Product: sigma-24 (FecI)

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MDHPRRLPSMSLMSHFLFGPFRPTLEALRPMLYRIAYAWCHDPALADDLVQEALSKAWARRAQLREDAALKGWVVSIMNH
CWVDHLRRRREFEDIEEWEDELESAGDSPEACCDRERVVACIRSAVAQLPPAQRQVVTLVDLEEFGYAEVAGILNIPVGT
VMSRLSRARANLKKRLDAAQTAAGPSLWRVK

Sequences:

>Translated_191_residues
MDHPRRLPSMSLMSHFLFGPFRPTLEALRPMLYRIAYAWCHDPALADDLVQEALSKAWARRAQLREDAALKGWVVSIMNH
CWVDHLRRRREFEDIEEWEDELESAGDSPEACCDRERVVACIRSAVAQLPPAQRQVVTLVDLEEFGYAEVAGILNIPVGT
VMSRLSRARANLKKRLDAAQTAAGPSLWRVK
>Mature_191_residues
MDHPRRLPSMSLMSHFLFGPFRPTLEALRPMLYRIAYAWCHDPALADDLVQEALSKAWARRAQLREDAALKGWVVSIMNH
CWVDHLRRRREFEDIEEWEDELESAGDSPEACCDRERVVACIRSAVAQLPPAQRQVVTLVDLEEFGYAEVAGILNIPVGT
VMSRLSRARANLKKRLDAAQTAAGPSLWRVK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=138, Percent_Identity=31.8840579710145, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21749; Mature: 21749

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHPRRLPSMSLMSHFLFGPFRPTLEALRPMLYRIAYAWCHDPALADDLVQEALSKAWAR
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RAQLREDAALKGWVVSIMNHCWVDHLRRRREFEDIEEWEDELESAGDSPEACCDRERVVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CIRSAVAQLPPAQRQVVTLVDLEEFGYAEVAGILNIPVGTVMSRLSRARANLKKRLDAAQ
HHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
TAAGPSLWRVK
HHCCCCCCCCC
>Mature Secondary Structure
MDHPRRLPSMSLMSHFLFGPFRPTLEALRPMLYRIAYAWCHDPALADDLVQEALSKAWAR
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RAQLREDAALKGWVVSIMNHCWVDHLRRRREFEDIEEWEDELESAGDSPEACCDRERVVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CIRSAVAQLPPAQRQVVTLVDLEEFGYAEVAGILNIPVGTVMSRLSRARANLKKRLDAAQ
HHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
TAAGPSLWRVK
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]