Click here to switch to the map view.

The map label for this gene is pilS [H]

Identifier: 74317824

GI number: 74317824

Start: 1908016

End: 1909875

Strand: Direct

Name: pilS [H]

Synonym: Tbd_1806

Alternate gene names: 74317824

Gene position: 1908016-1909875 (Clockwise)

Preceding gene: 74317820

Following gene: 74317825

Centisome position: 65.57

GC content: 68.06

Gene sequence:

>1860_bases
ATGAAACGGCGGGCGCCGGCGAGCGAAGGTATCCTTGGCGTCGACCTGTCTTTTGGAGATCCACGTGATCAAGATCCTGT
TGCTGATCGCCGCCGTATTCGTCGTCGTGGCGCTGCTGCGCACCTACCAGCGTTCGATCGCCCGCCCGTCGTCGTCGACC
AAGGACAAGGTAGAAGACATGGTGAAATGCGCCCACTGCGGGGTGAACCTGCCGCGCAGCGAGGCGATCTATTCCGGCGG
CGACTTCTACTGCACGCCCGAACACCAGCGCATCGGCAAAAAATGAGTCTCGCGGCCCCCATGCCGCGCCCGCCGACGCC
GGGCTATTTCGCGTCCCACTGGCGTAGCCTGGATTATTTCAACCTCTATCGCCTGACCCTCGCGGCCGCGCTGGTCTTCG
GCGCGCAGATGTTCTACGGCGACGCGAGCCTGTTTCGCTCCGATGCGGCGGAACGCTTCCAGATCATCGCCTACGGCTAC
CTCGTCGCGGCGGCGCTGTTCATTCTCGGGGTTCGCGCGCGCTGGCCGCGCTTCGACGTCCAGCTCACGATACACATCTT
CGCCGACATCGTGCTGGTGACGCTGATGATGTCGACCAGCGAGCGGCTCGCCGGCATGGGGCTCCTGCTCGTCATTTCGA
TCGCCTCCGGAGGCCTGGTCGGCCGGGGTCGTCTGACCCTGCTCTATGCCGCGGTCGCATCGATCGCGGTGCTGCTCCAG
CACGCCGTCAGCATCCTGCAGGGCGGTCAGGGCACCGACAGCCTCCTGCAGATCGCGCTGCTCTGCATGGGCTACTTCGC
CATCGGCATCCTCGCCCACGCGCTCACCCAGCGCGCGCTGCAGTCCGAGCGCCTGGCTCAGCAGCAGGCGGATGAACTGG
CGCTGCTCAACCGGATCAACGCGCTCGCGATCGAGAACTCCCCGGACGGCCTGCTTGCGATCCGCGGCGACGGCGTCCTG
CGCCACGCGAGCCCGCGCGCCCTCTCGCTGCTCGGCGTCACCGCGCCGATCCGGCCCGGACTGACCCGGCTCGACACCTG
CGCGCCCGCGCTCGCGCAACTGGCGCAGCGGGTCGAGCGCGGCTCGACCGCCGTCCTCGACACGCCGCTCGCCCGCCTGC
GGGTCCGCTGCATTCCGCTCGGCATTCCGGACGACAGCCGCGTGCTCGTGCTCGAGGACCAGAGCCAGGCCGAGCAAGCG
GCGCAGCGGCTCAAGCTCGCGGCCCTGGGCCGCCTGACCGCCAACATCGCGCACGAGATCCGCAACCCGCTGTCAGCCAT
CACCCAGGCCGCGCAGTTGCTGCAGGAAGACGCCCAAGGCCCGACGCAGACACGCTTGGTCGCGATCATCGAAAACAATG
CGCGCCGCCTCGACCGCCTGGTCGAGGAAGTCCTCACGCTCAATCGGCGCGACCGCCTGCGCCCGGTCCGGATCGACGGC
GCGGCCCTCGGCGCGATGATCGACGAATTGCGCCAGACTGAAGAAATCCCGGCGACTGCCGTCATAGTCGAGATGGCTGA
AAACGTTCGTTTCCACTTCGACGCCGACCACCTGCGCCAGATTCTCTGGAATCTGCTGCGCAACGCCTGGCGATTCAGCA
CGCGCGCGGCCGGCAGTATCCGCGTCAGCGCGCGCACCGTCGGCGAGACGCTCCAGCTCGACATCGCGGACGACGGCCCG
GGGGTGTCGCCGGAGCAGCAGGGAAAACTGTTCGAGCCCTTCTGCACGACCGACGCGCAGGGGACCGGACTCGGCCTCTT
CCTCGCCCGCGAACTGGCCGAAGCGAACCGCGCGGCGCTCGTCTACCTGCCCGGAGCCGGCGGCGCGAATTTCCGCCTGA
GCCTGCGTCAGGACACATGA

Upstream 100 bases:

>100_bases
ATTATGTAATTGTTTTAATTGGATATTTTTATGCGCTCGGGCCTTCTACTGCTGCGCATCGTGGGCTGGCGTAAAGAAAG
TCGACGAGCTTTTCTCATCG

Downstream 100 bases:

>100_bases
AACCCTCGAACCGCCCGCGCATCCTGCTCGTCGACGACGAGCCCGACCTGCTCGACCTGATGGAGCTCACGCTTCTCAAG
ATGGGCCTCGAAACCGACCG

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MKRRAPASEGILGVDLSFGDPRDQDPVADRRRIRRRGAAAHLPAFDRPPVVVDQGQGRRHGEMRPLRGEPAAQRGDLFRR
RLLLHARTPAHRQKMSLAAPMPRPPTPGYFASHWRSLDYFNLYRLTLAAALVFGAQMFYGDASLFRSDAAERFQIIAYGY
LVAAALFILGVRARWPRFDVQLTIHIFADIVLVTLMMSTSERLAGMGLLLVISIASGGLVGRGRLTLLYAAVASIAVLLQ
HAVSILQGGQGTDSLLQIALLCMGYFAIGILAHALTQRALQSERLAQQQADELALLNRINALAIENSPDGLLAIRGDGVL
RHASPRALSLLGVTAPIRPGLTRLDTCAPALAQLAQRVERGSTAVLDTPLARLRVRCIPLGIPDDSRVLVLEDQSQAEQA
AQRLKLAALGRLTANIAHEIRNPLSAITQAAQLLQEDAQGPTQTRLVAIIENNARRLDRLVEEVLTLNRRDRLRPVRIDG
AALGAMIDELRQTEEIPATAVIVEMAENVRFHFDADHLRQILWNLLRNAWRFSTRAAGSIRVSARTVGETLQLDIADDGP
GVSPEQQGKLFEPFCTTDAQGTGLGLFLARELAEANRAALVYLPGAGGANFRLSLRQDT

Sequences:

>Translated_619_residues
MKRRAPASEGILGVDLSFGDPRDQDPVADRRRIRRRGAAAHLPAFDRPPVVVDQGQGRRHGEMRPLRGEPAAQRGDLFRR
RLLLHARTPAHRQKMSLAAPMPRPPTPGYFASHWRSLDYFNLYRLTLAAALVFGAQMFYGDASLFRSDAAERFQIIAYGY
LVAAALFILGVRARWPRFDVQLTIHIFADIVLVTLMMSTSERLAGMGLLLVISIASGGLVGRGRLTLLYAAVASIAVLLQ
HAVSILQGGQGTDSLLQIALLCMGYFAIGILAHALTQRALQSERLAQQQADELALLNRINALAIENSPDGLLAIRGDGVL
RHASPRALSLLGVTAPIRPGLTRLDTCAPALAQLAQRVERGSTAVLDTPLARLRVRCIPLGIPDDSRVLVLEDQSQAEQA
AQRLKLAALGRLTANIAHEIRNPLSAITQAAQLLQEDAQGPTQTRLVAIIENNARRLDRLVEEVLTLNRRDRLRPVRIDG
AALGAMIDELRQTEEIPATAVIVEMAENVRFHFDADHLRQILWNLLRNAWRFSTRAAGSIRVSARTVGETLQLDIADDGP
GVSPEQQGKLFEPFCTTDAQGTGLGLFLARELAEANRAALVYLPGAGGANFRLSLRQDT
>Mature_619_residues
MKRRAPASEGILGVDLSFGDPRDQDPVADRRRIRRRGAAAHLPAFDRPPVVVDQGQGRRHGEMRPLRGEPAAQRGDLFRR
RLLLHARTPAHRQKMSLAAPMPRPPTPGYFASHWRSLDYFNLYRLTLAAALVFGAQMFYGDASLFRSDAAERFQIIAYGY
LVAAALFILGVRARWPRFDVQLTIHIFADIVLVTLMMSTSERLAGMGLLLVISIASGGLVGRGRLTLLYAAVASIAVLLQ
HAVSILQGGQGTDSLLQIALLCMGYFAIGILAHALTQRALQSERLAQQQADELALLNRINALAIENSPDGLLAIRGDGVL
RHASPRALSLLGVTAPIRPGLTRLDTCAPALAQLAQRVERGSTAVLDTPLARLRVRCIPLGIPDDSRVLVLEDQSQAEQA
AQRLKLAALGRLTANIAHEIRNPLSAITQAAQLLQEDAQGPTQTRLVAIIENNARRLDRLVEEVLTLNRRDRLRPVRIDG
AALGAMIDELRQTEEIPATAVIVEMAENVRFHFDADHLRQILWNLLRNAWRFSTRAAGSIRVSARTVGETLQLDIADDGP
GVSPEQQGKLFEPFCTTDAQGTGLGLFLARELAEANRAALVYLPGAGGANFRLSLRQDT

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=346, Percent_Identity=28.9017341040462, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=31.5555555555556, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790300, Length=253, Percent_Identity=30.0395256916996, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1786912, Length=251, Percent_Identity=26.6932270916335, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87082128, Length=223, Percent_Identity=25.5605381165919, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI48994928, Length=222, Percent_Identity=23.8738738738739, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1790346, Length=203, Percent_Identity=26.1083743842365, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786600, Length=344, Percent_Identity=22.6744186046512, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788393, Length=212, Percent_Identity=26.8867924528302, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1789808, Length=195, Percent_Identity=27.1794871794872, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1789149, Length=214, Percent_Identity=26.6355140186916, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1786783, Length=262, Percent_Identity=27.0992366412214, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=227, Percent_Identity=25.9911894273128, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081816, Length=214, Percent_Identity=28.0373831775701, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67666; Mature: 67666

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRAPASEGILGVDLSFGDPRDQDPVADRRRIRRRGAAAHLPAFDRPPVVVDQGQGRRH
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
GEMRPLRGEPAAQRGDLFRRRLLLHARTPAHRQKMSLAAPMPRPPTPGYFASHWRSLDYF
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCHH
NLYRLTLAAALVFGAQMFYGDASLFRSDAAERFQIIAYGYLVAAALFILGVRARWPRFDV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
QLTIHIFADIVLVTLMMSTSERLAGMGLLLVISIASGGLVGRGRLTLLYAAVASIAVLLQ
EEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHCCCCCCCCHHHHHHHHHHHHHHHHH
HAVSILQGGQGTDSLLQIALLCMGYFAIGILAHALTQRALQSERLAQQQADELALLNRIN
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAIENSPDGLLAIRGDGVLRHASPRALSLLGVTAPIRPGLTRLDTCAPALAQLAQRVER
HEEEECCCCCEEEEECCCEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHC
GSTAVLDTPLARLRVRCIPLGIPDDSRVLVLEDQSQAEQAAQRLKLAALGRLTANIAHEI
CCCEEECCCHHHHEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNPLSAITQAAQLLQEDAQGPTQTRLVAIIENNARRLDRLVEEVLTLNRRDRLRPVRIDG
HHHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCEEEECH
AALGAMIDELRQTEEIPATAVIVEMAENVRFHFDADHLRQILWNLLRNAWRFSTRAAGSI
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCEE
RVSARTVGETLQLDIADDGPGVSPEQQGKLFEPFCTTDAQGTGLGLFLARELAEANRAAL
EEEHEECCCEEEEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEE
VYLPGAGGANFRLSLRQDT
EEEECCCCCCEEEEEECCC
>Mature Secondary Structure
MKRRAPASEGILGVDLSFGDPRDQDPVADRRRIRRRGAAAHLPAFDRPPVVVDQGQGRRH
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
GEMRPLRGEPAAQRGDLFRRRLLLHARTPAHRQKMSLAAPMPRPPTPGYFASHWRSLDYF
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCHH
NLYRLTLAAALVFGAQMFYGDASLFRSDAAERFQIIAYGYLVAAALFILGVRARWPRFDV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
QLTIHIFADIVLVTLMMSTSERLAGMGLLLVISIASGGLVGRGRLTLLYAAVASIAVLLQ
EEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHCCCCCCCCHHHHHHHHHHHHHHHHH
HAVSILQGGQGTDSLLQIALLCMGYFAIGILAHALTQRALQSERLAQQQADELALLNRIN
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAIENSPDGLLAIRGDGVLRHASPRALSLLGVTAPIRPGLTRLDTCAPALAQLAQRVER
HEEEECCCCCEEEEECCCEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHC
GSTAVLDTPLARLRVRCIPLGIPDDSRVLVLEDQSQAEQAAQRLKLAALGRLTANIAHEI
CCCEEECCCHHHHEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNPLSAITQAAQLLQEDAQGPTQTRLVAIIENNARRLDRLVEEVLTLNRRDRLRPVRIDG
HHHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCEEEECH
AALGAMIDELRQTEEIPATAVIVEMAENVRFHFDADHLRQILWNLLRNAWRFSTRAAGSI
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCEE
RVSARTVGETLQLDIADDGPGVSPEQQGKLFEPFCTTDAQGTGLGLFLARELAEANRAAL
EEEHEECCCEEEEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEE
VYLPGAGGANFRLSLRQDT
EEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]