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The map label for this gene is abfA [H]

Identifier: 74317807

GI number: 74317807

Start: 1889047

End: 1890699

Strand: Reverse

Name: abfA [H]

Synonym: Tbd_1789

Alternate gene names: 74317807

Gene position: 1890699-1889047 (Counterclockwise)

Preceding gene: 74317808

Following gene: 74317806

Centisome position: 64.98

GC content: 56.14

Gene sequence:

>1653_bases
ATGCAAACAGTTACCGTACGCACCCGTCGCGGAATAAAGTCAGGTTTGACGGCGACAGCCACACGTTCCGTCCGAACCAG
CGCCTCAGCGCTCATATTGTGGGGGGGATTGCATGTGTCTGCCGACGCAGCGGAGCAAACGGCTTCGCAGTGGAAGCTGC
CGCGCGCTGTTCAGATTCAGCGGTCACAAGAGGTGCTGATCGAGGTAGACCCTAACAGTGTCATCCGAAGTGCCGTTCCC
GCTGCCCTGTTCGGTTTCAATATTCCTTGGATGAACTTTCAGCGGGGATATTGGCGGGAGAACCAAGTGAGGCCGGAGGT
CATTGCGTGGCTCAAACCGTTTTCCGGCGCCGTATATCGGTACCCCGGCGGGGAGTGGTCAAACTGGTTCGAGTGGGAAA
AGGCCGCGGGCCCGGGATCCTCGAGGCCCGAGCAGTATACGACCTTCGGATCCACTAAAGCGGAATTCGGTTTTGATGAG
TTCCTCGACTTTGTAAAAGCCGTGAATGGGGTGCCTCTGGTGACGGTTAATCTCAAGGGTACAAAGGGGGCTCCTTGGAG
TGACCAGCAAGCGGTGGAGAGCAATGTTGAATGGCTCCAACATTCTGTTCAGCGTGAGGGAAGAGCGGTTGCACGTGGTA
ACGCGCAGTTCTGCCAAACGGGAACAAAGTGTCCTGTCGAGTGGTGGGAACTGGGTAACGAGCTCGATTGGGGCAAAGGC
GCTTGGACACATACCGAGTACGTCAACCGGGCGCGGGAAGTCGGGCTCGCGATGAAAAATATCGATCCGGCCATCAAGCT
TATCGCTCACACAACGAGTTCGCCGTGGAGCACAAAACGCGTTATCGGGGAAAAGCCGAAGGCTAGGGATTTCGATAGCG
CCGTCGGCGCTGGGTTAGGAGATCTTGCCTATGGATATGCCTACCATCCCTACTATGACGGGATCAGTATTCCCGCTGCA
AATCACTACATGGAGCGCGCGATTAATTATTTGCGGCCTACTGCATCGACTGGCAAGCCTCCTCCCATATTTGTCACGGA
GCACGGCCGGTGGCCGAACCAACCCAAGTTCGGCAAGTGGGAAACCACTTGGATAAAAACCGGAAATTTAGGCGGGGCAG
TCTCGACTGCGGACTTCCTTCTCTCCCAAATGGTGATTCCGGATGTACGAGCCGCCATGTGGCATTCGCTCGGGGCGCGA
GGGCCGTGGCAATTGTTCTATCTTGACGCGGCCGCCGATGCTCTTTATCCAAACGTCGTTTATTGGGCATTACGGGTGCT
TCGCGAGGGGTTAATTGGCGACGCTCTAAAGGTGAGCGTTTCTTCTCCTAACACAAGCGGTTATCGCGGCGGCTACGATG
TCCGTGCCGTATTCATGCGCGACAGTACGCACACACGTTACAGCTTGCTAACGATTAATCGCGCTGAGAAAGAGCAGAAA
GCGCGCTTGCTCTTCCCGGAGTGGGCAGGCCGCAAGGTAAAGGGGCAACAGTACTACGTTAGTGGCGACAGTGATGCGCT
CGCGAACACGAAAGAACGCAAAAACGCTGTGACTATGCGGAGCCGTGCCGTCGGCCTCCGATTTGACGACGCAGGTCAGG
CCGAGATTAGCCTCCCAGCGTTTAGTGTGTCGAGTATCGTGATTACGCCTTGA

Upstream 100 bases:

>100_bases
GTTCCAGGACCCGGGGCTTTTTCTTGTTCCCGGAATTTTTCTTGGCGTCGCGTACAACCGCATGATGGCCTGGAAAAAAC
CCACAAAGCGAGGCTGACAT

Downstream 100 bases:

>100_bases
TCCCCTTAAAACCGCCCGCGCTGGCTAGTCGCCAAGCCGGAGTGCCTACTAAAGATGTGGCACGGGGAGCGAGGTTAATC
AGGTATCTGTGGCATGGACC

Product: putative alpha-L-arabinofuranosidase

Products: NA

Alternate protein names: ABF; Arabinosidase; Alpha-L-AF [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MQTVTVRTRRGIKSGLTATATRSVRTSASALILWGGLHVSADAAEQTASQWKLPRAVQIQRSQEVLIEVDPNSVIRSAVP
AALFGFNIPWMNFQRGYWRENQVRPEVIAWLKPFSGAVYRYPGGEWSNWFEWEKAAGPGSSRPEQYTTFGSTKAEFGFDE
FLDFVKAVNGVPLVTVNLKGTKGAPWSDQQAVESNVEWLQHSVQREGRAVARGNAQFCQTGTKCPVEWWELGNELDWGKG
AWTHTEYVNRAREVGLAMKNIDPAIKLIAHTTSSPWSTKRVIGEKPKARDFDSAVGAGLGDLAYGYAYHPYYDGISIPAA
NHYMERAINYLRPTASTGKPPPIFVTEHGRWPNQPKFGKWETTWIKTGNLGGAVSTADFLLSQMVIPDVRAAMWHSLGAR
GPWQLFYLDAAADALYPNVVYWALRVLREGLIGDALKVSVSSPNTSGYRGGYDVRAVFMRDSTHTRYSLLTINRAEKEQK
ARLLFPEWAGRKVKGQQYYVSGDSDALANTKERKNAVTMRSRAVGLRFDDAGQAEISLPAFSVSSIVITP

Sequences:

>Translated_550_residues
MQTVTVRTRRGIKSGLTATATRSVRTSASALILWGGLHVSADAAEQTASQWKLPRAVQIQRSQEVLIEVDPNSVIRSAVP
AALFGFNIPWMNFQRGYWRENQVRPEVIAWLKPFSGAVYRYPGGEWSNWFEWEKAAGPGSSRPEQYTTFGSTKAEFGFDE
FLDFVKAVNGVPLVTVNLKGTKGAPWSDQQAVESNVEWLQHSVQREGRAVARGNAQFCQTGTKCPVEWWELGNELDWGKG
AWTHTEYVNRAREVGLAMKNIDPAIKLIAHTTSSPWSTKRVIGEKPKARDFDSAVGAGLGDLAYGYAYHPYYDGISIPAA
NHYMERAINYLRPTASTGKPPPIFVTEHGRWPNQPKFGKWETTWIKTGNLGGAVSTADFLLSQMVIPDVRAAMWHSLGAR
GPWQLFYLDAAADALYPNVVYWALRVLREGLIGDALKVSVSSPNTSGYRGGYDVRAVFMRDSTHTRYSLLTINRAEKEQK
ARLLFPEWAGRKVKGQQYYVSGDSDALANTKERKNAVTMRSRAVGLRFDDAGQAEISLPAFSVSSIVITP
>Mature_550_residues
MQTVTVRTRRGIKSGLTATATRSVRTSASALILWGGLHVSADAAEQTASQWKLPRAVQIQRSQEVLIEVDPNSVIRSAVP
AALFGFNIPWMNFQRGYWRENQVRPEVIAWLKPFSGAVYRYPGGEWSNWFEWEKAAGPGSSRPEQYTTFGSTKAEFGFDE
FLDFVKAVNGVPLVTVNLKGTKGAPWSDQQAVESNVEWLQHSVQREGRAVARGNAQFCQTGTKCPVEWWELGNELDWGKG
AWTHTEYVNRAREVGLAMKNIDPAIKLIAHTTSSPWSTKRVIGEKPKARDFDSAVGAGLGDLAYGYAYHPYYDGISIPAA
NHYMERAINYLRPTASTGKPPPIFVTEHGRWPNQPKFGKWETTWIKTGNLGGAVSTADFLLSQMVIPDVRAAMWHSLGAR
GPWQLFYLDAAADALYPNVVYWALRVLREGLIGDALKVSVSSPNTSGYRGGYDVRAVFMRDSTHTRYSLLTINRAEKEQK
ARLLFPEWAGRKVKGQQYYVSGDSDALANTKERKNAVTMRSRAVGLRFDDAGQAEISLPAFSVSSIVITP

Specific function: Important role in the degradation of arabinose- containing hemicelluloses. Hydrolyzes rapidly the short-chain arabino-oligoxylosides from digestion of xylan with xylanases. Acts slowly on arabinan and arabinoxylan from wheat and rye flour to release L-ara

COG id: COG3534

COG function: function code G; Alpha-L-arabinofuranosidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 51 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010720
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF06964 Alpha-L-AF_C [H]

EC number: =3.2.1.55 [H]

Molecular weight: Translated: 61133; Mature: 61133

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVTVRTRRGIKSGLTATATRSVRTSASALILWGGLHVSADAAEQTASQWKLPRAVQIQ
CCEEEEHHHHCHHHCCCHHHHHHHHCCCCEEEEEECEEEECCHHHHHHHHCCCCCCEEEE
RSQEVLIEVDPNSVIRSAVPAALFGFNIPWMNFQRGYWRENQVRPEVIAWLKPFSGAVYR
CCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEE
YPGGEWSNWFEWEKAAGPGSSRPEQYTTFGSTKAEFGFDEFLDFVKAVNGVPLVTVNLKG
CCCCCCCCCHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECC
TKGAPWSDQQAVESNVEWLQHSVQREGRAVARGNAQFCQTGTKCPVEWWELGNELDWGKG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHCCCCCCHHHHHCCCCCCCCCC
AWTHTEYVNRAREVGLAMKNIDPAIKLIAHTTSSPWSTKRVIGEKPKARDFDSAVGAGLG
CCCHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCHHHHHCCCCCCCCCHHHHCCCCH
DLAYGYAYHPYYDGISIPAANHYMERAINYLRPTASTGKPPPIFVTEHGRWPNQPKFGKW
HHHHCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCE
ETTWIKTGNLGGAVSTADFLLSQMVIPDVRAAMWHSLGARGPWQLFYLDAAADALYPNVV
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHH
YWALRVLREGLIGDALKVSVSSPNTSGYRGGYDVRAVFMRDSTHTRYSLLTINRAEKEQK
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEEHHHHHHH
ARLLFPEWAGRKVKGQQYYVSGDSDALANTKERKNAVTMRSRAVGLRFDDAGQAEISLPA
CEEECHHHCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHEEEEEECCCCCCEEECCE
FSVSSIVITP
EEECEEEECC
>Mature Secondary Structure
MQTVTVRTRRGIKSGLTATATRSVRTSASALILWGGLHVSADAAEQTASQWKLPRAVQIQ
CCEEEEHHHHCHHHCCCHHHHHHHHCCCCEEEEEECEEEECCHHHHHHHHCCCCCCEEEE
RSQEVLIEVDPNSVIRSAVPAALFGFNIPWMNFQRGYWRENQVRPEVIAWLKPFSGAVYR
CCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEE
YPGGEWSNWFEWEKAAGPGSSRPEQYTTFGSTKAEFGFDEFLDFVKAVNGVPLVTVNLKG
CCCCCCCCCHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECC
TKGAPWSDQQAVESNVEWLQHSVQREGRAVARGNAQFCQTGTKCPVEWWELGNELDWGKG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHCCCCCCHHHHHCCCCCCCCCC
AWTHTEYVNRAREVGLAMKNIDPAIKLIAHTTSSPWSTKRVIGEKPKARDFDSAVGAGLG
CCCHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCHHHHHCCCCCCCCCHHHHCCCCH
DLAYGYAYHPYYDGISIPAANHYMERAINYLRPTASTGKPPPIFVTEHGRWPNQPKFGKW
HHHHCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCE
ETTWIKTGNLGGAVSTADFLLSQMVIPDVRAAMWHSLGARGPWQLFYLDAAADALYPNVV
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHH
YWALRVLREGLIGDALKVSVSSPNTSGYRGGYDVRAVFMRDSTHTRYSLLTINRAEKEQK
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEEHHHHHHH
ARLLFPEWAGRKVKGQQYYVSGDSDALANTKERKNAVTMRSRAVGLRFDDAGQAEISLPA
CEEECHHHCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHEEEEEECCCCCCEEECCE
FSVSSIVITP
EEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8092996 [H]