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The map label for this gene is rfbD [H]

Identifier: 74317795

GI number: 74317795

Start: 1876923

End: 1877816

Strand: Reverse

Name: rfbD [H]

Synonym: Tbd_1777

Alternate gene names: 74317795

Gene position: 1877816-1876923 (Counterclockwise)

Preceding gene: 74317796

Following gene: 74317794

Centisome position: 64.53

GC content: 68.9

Gene sequence:

>894_bases
GTGAGGCCGCGCATTCTGCTCACCGGGCCGAACGGCCAGGTCGGCTGGGAGTTGCAGCGCACGCTGGGCTCCCTCGGCGA
GGTCGTCGCCCTCGATTCGAGCGCGCTGAACCTCGCAGATCCGCGGCAGGTGCGCGAGACGGTGCGTGCGCTCGCCCCGC
GGATCATCGTCAATCCGGCTGCCTATACCGCGGTCGACAAGGCGGAAAGCGAACCCGAACTGGCGCGTGCCGTGAACGTC
GTGGCGCCCGCTCTGCTCGCCGAGGAAGCGCACCGGCTCGATGCCCTGCTCGTCCATTACTCGACAGACTACGTGTTCAA
CGGCAGCGGCAGCCGGCCGTGGCGCGAAGACGACCCTTGCGACCCGCTCAACGTGTATGGCGCGACGAAGCTCGAGGGTG
AGCGTGCGATCCAGGAGAGTGGCTGTCGCCACCTGATCTTCCGCACATCGTGGGTGTACGGCGCGCGAGGCAGCAACTTC
CTGCTGACGATGACGCGCCTCATGCGCGAACGGCCGGAACTCAAGATCGTCGCCGATCAGATGGGCGCCCCGACCTGGTG
TCGCGATCTCGCCGAGGCGACGGCGCAGATTCTGAGCCAGCTGTGCGCGCCCCAAACCCGATTGACCGATCCGGCGCCCT
GGGGCGTCTACCACGCTGCCAACGGCGGCGAGACGTCGTGGCACGGTTTTGCCGAAGCGATCCAGCGCCTCGAGGGCGAG
GGGCCCGCGGCGCATGCGCGGCTGCTGCCGATCCCGAGTAGCGACTACCCGACGCCAGCACGGCGGCCGCTCAATTCCCG
GCTCGACAGCGACAAGCTCGCGCGCGTTTTCGGCTTGCGCCTGCCCGACTGGCGCGTCGCGCTCGCGCTGTGTCTCGGCA
AATCCGCCGCCTGA

Upstream 100 bases:

>100_bases
ACGATCCCGAAATCGGCATCGAGTGGCCATGGAGCGGCTCGCCGACCCTCTCGGCCAAGGACCAGGTCTTGCCCTTTCTC
AAGGACGCCGAGGTCTACGC

Downstream 100 bases:

>100_bases
CGCGCCTCGGCGCCGCGGCGAAAGAATGACGGTCGTCCGAAAAAAATAGGACAATTGCGGACTTCGTGATTGTCGTGAGC
CCAGGAACATGATCAAGAAA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRPRILLTGPNGQVGWELQRTLGSLGEVVALDSSALNLADPRQVRETVRALAPRIIVNPAAYTAVDKAESEPELARAVNV
VAPALLAEEAHRLDALLVHYSTDYVFNGSGSRPWREDDPCDPLNVYGATKLEGERAIQESGCRHLIFRTSWVYGARGSNF
LLTMTRLMRERPELKIVADQMGAPTWCRDLAEATAQILSQLCAPQTRLTDPAPWGVYHAANGGETSWHGFAEAIQRLEGE
GPAAHARLLPIPSSDYPTPARRPLNSRLDSDKLARVFGLRLPDWRVALALCLGKSAA

Sequences:

>Translated_297_residues
MRPRILLTGPNGQVGWELQRTLGSLGEVVALDSSALNLADPRQVRETVRALAPRIIVNPAAYTAVDKAESEPELARAVNV
VAPALLAEEAHRLDALLVHYSTDYVFNGSGSRPWREDDPCDPLNVYGATKLEGERAIQESGCRHLIFRTSWVYGARGSNF
LLTMTRLMRERPELKIVADQMGAPTWCRDLAEATAQILSQLCAPQTRLTDPAPWGVYHAANGGETSWHGFAEAIQRLEGE
GPAAHARLLPIPSSDYPTPARRPLNSRLDSDKLARVFGLRLPDWRVALALCLGKSAA
>Mature_297_residues
MRPRILLTGPNGQVGWELQRTLGSLGEVVALDSSALNLADPRQVRETVRALAPRIIVNPAAYTAVDKAESEPELARAVNV
VAPALLAEEAHRLDALLVHYSTDYVFNGSGSRPWREDDPCDPLNVYGATKLEGERAIQESGCRHLIFRTSWVYGARGSNF
LLTMTRLMRERPELKIVADQMGAPTWCRDLAEATAQILSQLCAPQTRLTDPAPWGVYHAANGGETSWHGFAEAIQRLEGE
GPAAHARLLPIPSSDYPTPARRPLNSRLDSDKLARVFGLRLPDWRVALALCLGKSAA

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=303, Percent_Identity=29.7029702970297, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI33519455, Length=303, Percent_Identity=29.7029702970297, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1788352, Length=297, Percent_Identity=47.4747474747475, Blast_Score=272, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32530; Mature: 32530

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRILLTGPNGQVGWELQRTLGSLGEVVALDSSALNLADPRQVRETVRALAPRIIVNPA
CCCCEEEECCCCCCCHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHCCCEEECCH
AYTAVDKAESEPELARAVNVVAPALLAEEAHRLDALLVHYSTDYVFNGSGSRPWREDDPC
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEECCCCCCCCCCCCCC
DPLNVYGATKLEGERAIQESGCRHLIFRTSWVYGARGSNFLLTMTRLMRERPELKIVADQ
CCCCCCCCCCCCHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEHHH
MGAPTWCRDLAEATAQILSQLCAPQTRLTDPAPWGVYHAANGGETSWHGFAEAIQRLEGE
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCC
GPAAHARLLPIPSSDYPTPARRPLNSRLDSDKLARVFGLRLPDWRVALALCLGKSAA
CCCCCEEEEECCCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRPRILLTGPNGQVGWELQRTLGSLGEVVALDSSALNLADPRQVRETVRALAPRIIVNPA
CCCCEEEECCCCCCCHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHCCCEEECCH
AYTAVDKAESEPELARAVNVVAPALLAEEAHRLDALLVHYSTDYVFNGSGSRPWREDDPC
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEECCCCCCCCCCCCCC
DPLNVYGATKLEGERAIQESGCRHLIFRTSWVYGARGSNFLLTMTRLMRERPELKIVADQ
CCCCCCCCCCCCHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEHHH
MGAPTWCRDLAEATAQILSQLCAPQTRLTDPAPWGVYHAANGGETSWHGFAEAIQRLEGE
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCC
GPAAHARLLPIPSSDYPTPARRPLNSRLDSDKLARVFGLRLPDWRVALALCLGKSAA
CCCCCEEEEECCCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]