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The map label for this gene is rfbD [H]

Identifier: 74317794

GI number: 74317794

Start: 1875749

End: 1876834

Strand: Reverse

Name: rfbD [H]

Synonym: Tbd_1776

Alternate gene names: 74317794

Gene position: 1876834-1875749 (Counterclockwise)

Preceding gene: 74317795

Following gene: 74317793

Centisome position: 64.5

GC content: 62.89

Gene sequence:

>1086_bases
ATGATCAAGAAAGCCCTCATCACCGGCGTGACCGGCCAGGACGGCGCCTATCTCGCCGAATTCCTGCTCGGCAAGGGCTA
CGAGGTCCACGGCATCAAGCGCCGCACCTCGCTTTTCAACACCGACCGCATCGACCATCTCTACCAGGACCCGCACGAGA
AGGACCGCCGCTTCATCCTGCATCACGGCGACCTTACCGACTCGTCGAGCCTGATCCGCATCATCCAGCAGGTGCGGCCC
GACGAGATCTACAACCTCGCCGCGCAGTCGCACGTCGCCGTTTCGTTCGAGGAGCCCGAGTACACCGCCAACTCTGACGC
GCTCGGCGCGCTGCGCATCCTCGAGGCCATCCGCATCCTCGGCCTCGAGAAAAAGACGCGCTTCTACCAGGCCAGCACCA
GCGAACTCTACGGCCTGGTGCAGGAAACCCCGCAGAAGGAGACCACCCCCTTCTATCCGCGCAGCCCCTACGCGGTCGCC
AAGCTCTACGCCTACTGGATCACGGTCAACTACCGCGAGGCTTACGGCATGTATGCCTGCAACGGCATCCTCTTCAACCA
CGAGTCGCCGATCCGTGGGGAGACCTTTGTTACCCGCAAGATCACGCGCGCGCTGGCCCGAATCAAACTGGGCCTGCAGG
ACTGTCTCTTCCTCGGCAACATGGACGCCAAACGCGACTGGGGCCACGCCAAGGACTACGTCGAGATGCAGTGGCTGATG
CTGCAGCAGGAGAAGCCCGAGGACTTCGTCATCGCCACCGGCGTGCAGTACAGCGTGCGCGACTTCGTCGACGCCGCCGC
GAAGGAACTCGGCATGAGCATCCGCTGGGAAGGAGAGGGCGTCGCCGAGAAGGGCTACGACGACAAAGGCCGCTGCGTCG
TCGCCGTCGACCCGCGCTACTTCCGACCGACCGAAGTCGAAACCCTGCTTGGTGATCCCGCCAAGGCCAAGGACAAGCTC
GGCTGGACGCCGAAGATCAGCTTCGACGAACTCGTCACCGAAATGGTGCGCGAAGACCTCAAGGCGGCCGAGCGCGACGA
GCTGGTGAAAAAGCACGGCTACAAAGCCATGGATTACCACGAATAA

Upstream 100 bases:

>100_bases
TCCGCCGCCTGACGCGCCTCGGCGCCGCGGCGAAAGAATGACGGTCGTCCGAAAAAAATAGGACAATTGCGGACTTCGTG
ATTGTCGTGAGCCCAGGAAC

Downstream 100 bases:

>100_bases
ACGATGGACAAGAACGCGCGCATTTATGTGGCGGGGCACCGCGGCCTCGTCGGCTCCGCGCTGATGCGCGATCTCGAACG
CAAGGGCTACGGCAACTTCG

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MIKKALITGVTGQDGAYLAEFLLGKGYEVHGIKRRTSLFNTDRIDHLYQDPHEKDRRFILHHGDLTDSSSLIRIIQQVRP
DEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAIRILGLEKKTRFYQASTSELYGLVQETPQKETTPFYPRSPYAVA
KLYAYWITVNYREAYGMYACNGILFNHESPIRGETFVTRKITRALARIKLGLQDCLFLGNMDAKRDWGHAKDYVEMQWLM
LQQEKPEDFVIATGVQYSVRDFVDAAAKELGMSIRWEGEGVAEKGYDDKGRCVVAVDPRYFRPTEVETLLGDPAKAKDKL
GWTPKISFDELVTEMVREDLKAAERDELVKKHGYKAMDYHE

Sequences:

>Translated_361_residues
MIKKALITGVTGQDGAYLAEFLLGKGYEVHGIKRRTSLFNTDRIDHLYQDPHEKDRRFILHHGDLTDSSSLIRIIQQVRP
DEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAIRILGLEKKTRFYQASTSELYGLVQETPQKETTPFYPRSPYAVA
KLYAYWITVNYREAYGMYACNGILFNHESPIRGETFVTRKITRALARIKLGLQDCLFLGNMDAKRDWGHAKDYVEMQWLM
LQQEKPEDFVIATGVQYSVRDFVDAAAKELGMSIRWEGEGVAEKGYDDKGRCVVAVDPRYFRPTEVETLLGDPAKAKDKL
GWTPKISFDELVTEMVREDLKAAERDELVKKHGYKAMDYHE
>Mature_361_residues
MIKKALITGVTGQDGAYLAEFLLGKGYEVHGIKRRTSLFNTDRIDHLYQDPHEKDRRFILHHGDLTDSSSLIRIIQQVRP
DEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAIRILGLEKKTRFYQASTSELYGLVQETPQKETTPFYPRSPYAVA
KLYAYWITVNYREAYGMYACNGILFNHESPIRGETFVTRKITRALARIKLGLQDCLFLGNMDAKRDWGHAKDYVEMQWLM
LQQEKPEDFVIATGVQYSVRDFVDAAAKELGMSIRWEGEGVAEKGYDDKGRCVVAVDPRYFRPTEVETLLGDPAKAKDKL
GWTPKISFDELVTEMVREDLKAAERDELVKKHGYKAMDYHE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=339, Percent_Identity=62.8318584070796, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI7657641, Length=335, Percent_Identity=24.1791044776119, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788366, Length=365, Percent_Identity=73.1506849315068, Blast_Score=566, Evalue=1e-163,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=26.2247838616715, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788353, Length=374, Percent_Identity=23.2620320855615, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=348, Percent_Identity=64.6551724137931, Blast_Score=469, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17539424, Length=340, Percent_Identity=64.1176470588235, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901790, Length=340, Percent_Identity=64.1176470588235, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17539422, Length=340, Percent_Identity=64.1176470588235, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901788, Length=340, Percent_Identity=64.1176470588235, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901786, Length=340, Percent_Identity=64.1176470588235, Blast_Score=463, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24158427, Length=348, Percent_Identity=62.9310344827586, Blast_Score=459, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 41336; Mature: 41336

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKALITGVTGQDGAYLAEFLLGKGYEVHGIKRRTSLFNTDRIDHLYQDPHEKDRRFIL
CCCCCEEECCCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCHHHHHHHCCCCCCCCEEEE
HHGDLTDSSSLIRIIQQVRPDEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAIRIL
EECCCCCHHHHHHHHHHHCHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
GLEKKTRFYQASTSELYGLVQETPQKETTPFYPRSPYAVAKLYAYWITVNYREAYGMYAC
CCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEHHHHCCEEEE
NGILFNHESPIRGETFVTRKITRALARIKLGLQDCLFLGNMDAKRDWGHAKDYVEMQWLM
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LQQEKPEDFVIATGVQYSVRDFVDAAAKELGMSIRWEGEGVAEKGYDDKGRCVVAVDPRY
HHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEEECCCC
FRPTEVETLLGDPAKAKDKLGWTPKISFDELVTEMVREDLKAAERDELVKKHGYKAMDYH
CCCCHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
E
C
>Mature Secondary Structure
MIKKALITGVTGQDGAYLAEFLLGKGYEVHGIKRRTSLFNTDRIDHLYQDPHEKDRRFIL
CCCCCEEECCCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCHHHHHHHCCCCCCCCEEEE
HHGDLTDSSSLIRIIQQVRPDEIYNLAAQSHVAVSFEEPEYTANSDALGALRILEAIRIL
EECCCCCHHHHHHHHHHHCHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
GLEKKTRFYQASTSELYGLVQETPQKETTPFYPRSPYAVAKLYAYWITVNYREAYGMYAC
CCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEHHHHCCEEEE
NGILFNHESPIRGETFVTRKITRALARIKLGLQDCLFLGNMDAKRDWGHAKDYVEMQWLM
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LQQEKPEDFVIATGVQYSVRDFVDAAAKELGMSIRWEGEGVAEKGYDDKGRCVVAVDPRY
HHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEEECCCC
FRPTEVETLLGDPAKAKDKLGWTPKISFDELVTEMVREDLKAAERDELVKKHGYKAMDYH
CCCCHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9098074; 10521656 [H]