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The map label for this gene is fixL [H]

Identifier: 74317762

GI number: 74317762

Start: 1831479

End: 1833371

Strand: Reverse

Name: fixL [H]

Synonym: Tbd_1744

Alternate gene names: 74317762

Gene position: 1833371-1831479 (Counterclockwise)

Preceding gene: 74317763

Following gene: 74317761

Centisome position: 63.01

GC content: 70.21

Gene sequence:

>1893_bases
ATGTCGATAAACTGGGCGAACGCGCGCTGCACGTTCCATGCCGGTCCATCGCCAAGCCATCGACAGCGCGCCAGCCAGAT
CGAAAGGAGCGCACGGATCAATTCATTGCGGCGAATCCAGCGGATCGTCATCGGCATTCTGGCCGTGCTCGTTTTCGTAC
CCGCCGCGCGGGCGCAGGCCGAAGCCGCTCCGGTGCGGATCGGCGTGCTCGCCTTTCTTGGCTGGGAGGCGGCGTTTTCC
GACTGGGGGCCGCTCGCGCAGCATCTCGGGTCGGCCCTGCCGGGACGGCGTTTCGCGCTCGCCTACTACGACGCGGCCGG
CTTGCGCGAGGCGGTGCGTCGCGAGGAAGTCGATTTCGTCATCACCAACAGCGGCCACTACGCCGCGCTCGAATCGGAGC
TCGGGATCAGCCGCATCGCCACGCTCGATTCGCCGAGCGTGCCGTCCCCCGCGCGGGCGATCGGTTCGGCAGTGATCGCC
CGCGCAGATCGTGATGATCTCGAGGTGCTCGCCGATCTCAAGGGCCAGCGTGTCGCGGCCGTGGGCGAGGAGGCGTTCGG
CGGGTTTCAGGTGGCATGGCGCGAACTGCGGCGGCAGGGGATCGAACCGGAGGACTTCGCGCAGCTCGATTTCCTCGGCT
TTCCGATATCGCGCATCGTGACCGCGATCGCCGACGGCGAGGCCGACGCCGGAATCGTGCGCGCGTGCCTGCTCGAGGAA
CTGGCGCGCAGCGGCGCCGTGCGGCTCGCCGACTTCAAGGTGCTCTCACCGCGCCGCGAGCCCGGCTTCAGCTGCGGCCT
GTCGACCGAGCTCTATCCCGATTGGCCGATCGCGACGCTGCGTCACACCGATCTGCGCCTCGCCAAGGCGGTCGCCGCCG
CGCTGCTGGCGATGCCGCCCACGCCCAAGGGCTACGCCTGGACCGCGCCGGTCGACTACCGGCCGGTGCACGACTTGTTT
CGCGACCTCGAGATCGGGCCCTACAGCTATCTGGGCGAGAACACGCTGCAGGCGCTCGCCCGCCGTCACTGGCCGTTCCT
GCTGTTCTTTTTCGCGGCGCTGGGGGGCTGGGTGATACACGCCGTTCGCGTCGAGCAGCAGGTCCACGCCCGCACGGCCG
CGCTGCGCGCAGCGCTGGCCGAGCGCGACGCGGCGGAGGCCGGCATGCGCGCGCACCAGGAGCAGCTCGATCATCTCTCG
CGTCTCTCGGTCCTCGGCGAGCTCTCGGGAACGCTCGCCCACGAACTGAACCAGCCGCTCGCCGCGATCGGCAACTACGC
GCAGAGCCTGCTGCGGCGGCTCGAGAGCGGGCGTTATACCCCCGCGATCTTGGCCGAGGCGAGCGAGGAAATCGCCGTGC
AGGCCGAGCGCGCCGGCGGCATCGTGCGCCGCATCCGCGCGTTCGCGAGCAAGCGCGCGGCCGTACGCGAAAAGCGCCCG
CTGCGCGAAGTCGTGCAGGAGGCGACGACGCTGTTCGGCGCGATGCTGCCCGCCGCGCCGCGCGTCGAGATCGACGACCG
CCTGCCGGCCGGCGCGGTCGTCGACGCCGACGTGCTGCAGATGCAGCAGGTTCTGCTCAACCTGCTCAAAAACGCCGCCG
ACGCGATGCAGGAGCTTGCGCCGCGCGAGCGCGCGATCGGGCTCGTGCTCGACCAGGTGGGACCTCACTACCGGATCGGC
GTGCGCGACCGCGGCGCCGGCGTGCCGGCGGGCTCGGCGCAGCACCTGTTCGAGCCCTTTTTTACGACCAAGCCCGACGG
CATGGGACTCGGCCTGTCGATCTGCAAGACCATCGTCGAGGCCCACGGCGGTCGCCTGTGGGCCGAGGCCAACGCCGCCC
CGCCCGGCATGACTTTTTTCTTCACGCTGCCCGCCGATGCCCAGCTCGCCTGA

Upstream 100 bases:

>100_bases
TAGGCCGTCGGCAGCGCGCGTCGCCGAGGCCGACGCGGGTCGCCCTCAGCGCGCGGTCGCTCGACGAATGCGCGACCGGC
CGGGCTGACGCCAGGCGAGC

Downstream 100 bases:

>100_bases
CACCGCTCTGATCCATATCGTCGACGACGACGAAGCACTGCGCCGCTCGCTGCTGTTCCTGTTCGATTCGGTCGGCTGGC
GCGCGCAGGCCTACGCCAGC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MSINWANARCTFHAGPSPSHRQRASQIERSARINSLRRIQRIVIGILAVLVFVPAARAQAEAAPVRIGVLAFLGWEAAFS
DWGPLAQHLGSALPGRRFALAYYDAAGLREAVRREEVDFVITNSGHYAALESELGISRIATLDSPSVPSPARAIGSAVIA
RADRDDLEVLADLKGQRVAAVGEEAFGGFQVAWRELRRQGIEPEDFAQLDFLGFPISRIVTAIADGEADAGIVRACLLEE
LARSGAVRLADFKVLSPRREPGFSCGLSTELYPDWPIATLRHTDLRLAKAVAAALLAMPPTPKGYAWTAPVDYRPVHDLF
RDLEIGPYSYLGENTLQALARRHWPFLLFFFAALGGWVIHAVRVEQQVHARTAALRAALAERDAAEAGMRAHQEQLDHLS
RLSVLGELSGTLAHELNQPLAAIGNYAQSLLRRLESGRYTPAILAEASEEIAVQAERAGGIVRRIRAFASKRAAVREKRP
LREVVQEATTLFGAMLPAAPRVEIDDRLPAGAVVDADVLQMQQVLLNLLKNAADAMQELAPRERAIGLVLDQVGPHYRIG
VRDRGAGVPAGSAQHLFEPFFTTKPDGMGLGLSICKTIVEAHGGRLWAEANAAPPGMTFFFTLPADAQLA

Sequences:

>Translated_630_residues
MSINWANARCTFHAGPSPSHRQRASQIERSARINSLRRIQRIVIGILAVLVFVPAARAQAEAAPVRIGVLAFLGWEAAFS
DWGPLAQHLGSALPGRRFALAYYDAAGLREAVRREEVDFVITNSGHYAALESELGISRIATLDSPSVPSPARAIGSAVIA
RADRDDLEVLADLKGQRVAAVGEEAFGGFQVAWRELRRQGIEPEDFAQLDFLGFPISRIVTAIADGEADAGIVRACLLEE
LARSGAVRLADFKVLSPRREPGFSCGLSTELYPDWPIATLRHTDLRLAKAVAAALLAMPPTPKGYAWTAPVDYRPVHDLF
RDLEIGPYSYLGENTLQALARRHWPFLLFFFAALGGWVIHAVRVEQQVHARTAALRAALAERDAAEAGMRAHQEQLDHLS
RLSVLGELSGTLAHELNQPLAAIGNYAQSLLRRLESGRYTPAILAEASEEIAVQAERAGGIVRRIRAFASKRAAVREKRP
LREVVQEATTLFGAMLPAAPRVEIDDRLPAGAVVDADVLQMQQVLLNLLKNAADAMQELAPRERAIGLVLDQVGPHYRIG
VRDRGAGVPAGSAQHLFEPFFTTKPDGMGLGLSICKTIVEAHGGRLWAEANAAPPGMTFFFTLPADAQLA
>Mature_629_residues
SINWANARCTFHAGPSPSHRQRASQIERSARINSLRRIQRIVIGILAVLVFVPAARAQAEAAPVRIGVLAFLGWEAAFSD
WGPLAQHLGSALPGRRFALAYYDAAGLREAVRREEVDFVITNSGHYAALESELGISRIATLDSPSVPSPARAIGSAVIAR
ADRDDLEVLADLKGQRVAAVGEEAFGGFQVAWRELRRQGIEPEDFAQLDFLGFPISRIVTAIADGEADAGIVRACLLEEL
ARSGAVRLADFKVLSPRREPGFSCGLSTELYPDWPIATLRHTDLRLAKAVAAALLAMPPTPKGYAWTAPVDYRPVHDLFR
DLEIGPYSYLGENTLQALARRHWPFLLFFFAALGGWVIHAVRVEQQVHARTAALRAALAERDAAEAGMRAHQEQLDHLSR
LSVLGELSGTLAHELNQPLAAIGNYAQSLLRRLESGRYTPAILAEASEEIAVQAERAGGIVRRIRAFASKRAAVREKRPL
REVVQEATTLFGAMLPAAPRVEIDDRLPAGAVVDADVLQMQQVLLNLLKNAADAMQELAPRERAIGLVLDQVGPHYRIGV
RDRGAGVPAGSAQHLFEPFFTTKPDGMGLGLSICKTIVEAHGGRLWAEANAAPPGMTFFFTLPADAQLA

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=242, Percent_Identity=29.3388429752066, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=27.8481012658228, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1790300, Length=244, Percent_Identity=31.9672131147541, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=229, Percent_Identity=27.0742358078603, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 68374; Mature: 68243

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINWANARCTFHAGPSPSHRQRASQIERSARINSLRRIQRIVIGILAVLVFVPAARAQA
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
EAAPVRIGVLAFLGWEAAFSDWGPLAQHLGSALPGRRFALAYYDAAGLREAVRREEVDFV
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHCCCCEE
ITNSGHYAALESELGISRIATLDSPSVPSPARAIGSAVIARADRDDLEVLADLKGQRVAA
EECCCCEEEEHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEH
VGEEAFGGFQVAWRELRRQGIEPEDFAQLDFLGFPISRIVTAIADGEADAGIVRACLLEE
HCHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHH
LARSGAVRLADFKVLSPRREPGFSCGLSTELYPDWPIATLRHTDLRLAKAVAAALLAMPP
HHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TPKGYAWTAPVDYRPVHDLFRDLEIGPYSYLGENTLQALARRHWPFLLFFFAALGGWVIH
CCCCCEEECCCCCCHHHHHHHHCCCCCHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AVRVEQQVHARTAALRAALAERDAAEAGMRAHQEQLDHLSRLSVLGELSGTLAHELNQPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AAIGNYAQSLLRRLESGRYTPAILAEASEEIAVQAERAGGIVRRIRAFASKRAAVREKRP
HHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LREVVQEATTLFGAMLPAAPRVEIDDRLPAGAVVDADVLQMQQVLLNLLKNAADAMQELA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PRERAIGLVLDQVGPHYRIGVRDRGAGVPAGSAQHLFEPFFTTKPDGMGLGLSICKTIVE
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AHGGRLWAEANAAPPGMTFFFTLPADAQLA
HCCCEEEEECCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
SINWANARCTFHAGPSPSHRQRASQIERSARINSLRRIQRIVIGILAVLVFVPAARAQA
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
EAAPVRIGVLAFLGWEAAFSDWGPLAQHLGSALPGRRFALAYYDAAGLREAVRREEVDFV
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHCCCCEE
ITNSGHYAALESELGISRIATLDSPSVPSPARAIGSAVIARADRDDLEVLADLKGQRVAA
EECCCCEEEEHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEH
VGEEAFGGFQVAWRELRRQGIEPEDFAQLDFLGFPISRIVTAIADGEADAGIVRACLLEE
HCHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHH
LARSGAVRLADFKVLSPRREPGFSCGLSTELYPDWPIATLRHTDLRLAKAVAAALLAMPP
HHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TPKGYAWTAPVDYRPVHDLFRDLEIGPYSYLGENTLQALARRHWPFLLFFFAALGGWVIH
CCCCCEEECCCCCCHHHHHHHHCCCCCHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AVRVEQQVHARTAALRAALAERDAAEAGMRAHQEQLDHLSRLSVLGELSGTLAHELNQPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AAIGNYAQSLLRRLESGRYTPAILAEASEEIAVQAERAGGIVRRIRAFASKRAAVREKRP
HHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LREVVQEATTLFGAMLPAAPRVEIDDRLPAGAVVDADVLQMQQVLLNLLKNAADAMQELA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PRERAIGLVLDQVGPHYRIGVRDRGAGVPAGSAQHLFEPFFTTKPDGMGLGLSICKTIVE
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AHGGRLWAEANAAPPGMTFFFTLPADAQLA
HCCCEEEEECCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]