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The map label for this gene is nodW [H]

Identifier: 74317761

GI number: 74317761

Start: 1830866

End: 1831495

Strand: Reverse

Name: nodW [H]

Synonym: Tbd_1743

Alternate gene names: 74317761

Gene position: 1831495-1830866 (Counterclockwise)

Preceding gene: 74317762

Following gene: 74317756

Centisome position: 62.94

GC content: 68.89

Gene sequence:

>630_bases
ATGCCCAGCTCGCCTGACACCGCTCTGATCCATATCGTCGACGACGACGAAGCACTGCGCCGCTCGCTGCTGTTCCTGTT
CGATTCGGTCGGCTGGCGCGCGCAGGCCTACGCCAGCGCCGACGAATTTCTTGCCGCGTACCGGCCCGGCGCGCCCGGCT
GCCTCGTGCTCGATATCCGCATGCCGGGCATGAGCGGGCTCGAACTGCAGCAGGTTCTGCGCGCGCGCGGCGAGGTGCTG
CCGGTGATTTTCATCACCGGGCACGGCGACGTCTCGCTGGCCGTGCAGGCGATGAAGCAGGAGGCCTGCGATTTTCTGGA
AAAGCCGTTCAAGGATCAGGCGCTGCTCGACGCGGTCGGGCAGGCGGTCCGGCGCAGCCGCGACGATCTCGCCGTCGAGG
CGCGCCGCAACCAGGCGCATGCGCTGCTCGAGCGACTGTCGCCGCGCGAGCGCGAGGTCGCGCGCCGCGTCGCGCGCGGC
CTGCCGAACAAGCTCATCGCGCGCGAACTCGACATCAGCGAAAAGACGGTGCACGTGCACCGCCAGCACGTCATGGAAAA
GACGCACACCGGGTCGGCCGCCGAATTGGCGCGGCTGATGCTGCGCGCCGATCCGGCCGATCTCGACTGA

Upstream 100 bases:

>100_bases
TCGATCTGCAAGACCATCGTCGAGGCCCACGGCGGTCGCCTGTGGGCCGAGGCCAACGCCGCCCCGCCCGGCATGACTTT
TTTCTTCACGCTGCCCGCCG

Downstream 100 bases:

>100_bases
GCGGCGGACCGCTCGGGCCCTGCCCGCCGCGCCTGCCTAAAACCCGCGCCGACGCAACTTCTGCACACCCTGCACGTAGG
TGAGCGCCTTCGCCAGCTCC

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MPSSPDTALIHIVDDDEALRRSLLFLFDSVGWRAQAYASADEFLAAYRPGAPGCLVLDIRMPGMSGLELQQVLRARGEVL
PVIFITGHGDVSLAVQAMKQEACDFLEKPFKDQALLDAVGQAVRRSRDDLAVEARRNQAHALLERLSPREREVARRVARG
LPNKLIARELDISEKTVHVHRQHVMEKTHTGSAAELARLMLRADPADLD

Sequences:

>Translated_209_residues
MPSSPDTALIHIVDDDEALRRSLLFLFDSVGWRAQAYASADEFLAAYRPGAPGCLVLDIRMPGMSGLELQQVLRARGEVL
PVIFITGHGDVSLAVQAMKQEACDFLEKPFKDQALLDAVGQAVRRSRDDLAVEARRNQAHALLERLSPREREVARRVARG
LPNKLIARELDISEKTVHVHRQHVMEKTHTGSAAELARLMLRADPADLD
>Mature_208_residues
PSSPDTALIHIVDDDEALRRSLLFLFDSVGWRAQAYASADEFLAAYRPGAPGCLVLDIRMPGMSGLELQQVLRARGEVLP
VIFITGHGDVSLAVQAMKQEACDFLEKPFKDQALLDAVGQAVRRSRDDLAVEARRNQAHALLERLSPREREVARRVARGL
PNKLIARELDISEKTVHVHRQHVMEKTHTGSAAELARLMLRADPADLD

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=103, Percent_Identity=38.8349514563107, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1790102, Length=169, Percent_Identity=36.6863905325444, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787473, Length=130, Percent_Identity=36.1538461538462, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23204; Mature: 23073

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSPDTALIHIVDDDEALRRSLLFLFDSVGWRAQAYASADEFLAAYRPGAPGCLVLDIR
CCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHCCCCCCCEEEEEEE
MPGMSGLELQQVLRARGEVLPVIFITGHGDVSLAVQAMKQEACDFLEKPFKDQALLDAVG
CCCCCCHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QAVRRSRDDLAVEARRNQAHALLERLSPREREVARRVARGLPNKLIARELDISEKTVHVH
HHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHH
RQHVMEKTHTGSAAELARLMLRADPADLD
HHHHHHHHCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PSSPDTALIHIVDDDEALRRSLLFLFDSVGWRAQAYASADEFLAAYRPGAPGCLVLDIR
CCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHCCCCCCCEEEEEEE
MPGMSGLELQQVLRARGEVLPVIFITGHGDVSLAVQAMKQEACDFLEKPFKDQALLDAVG
CCCCCCHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QAVRRSRDDLAVEARRNQAHALLERLSPREREVARRVARGLPNKLIARELDISEKTVHVH
HHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHH
RQHVMEKTHTGSAAELARLMLRADPADLD
HHHHHHHHCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]