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The map label for this gene is hisH

Identifier: 74317728

GI number: 74317728

Start: 1798103

End: 1798789

Strand: Reverse

Name: hisH

Synonym: Tbd_1710

Alternate gene names: 74317728

Gene position: 1798789-1798103 (Counterclockwise)

Preceding gene: 74317729

Following gene: 74317727

Centisome position: 61.82

GC content: 65.94

Gene sequence:

>687_bases
GTGAGCGTCGATATCGCCGTCGTCGACTACGGCATGGGCAACCTGCGCTCGGTGTCCAAGGCGATCGAGCACGTCGCGCC
GTCCGCCACGGTGGTCGTCACCTCCAGTCCGGACGTGATCGCCGAAGCCGGTCGGGTCGTGTTCCCGGGCCAGGGCGCGG
CCCGCGACTGTATGCGCGAGATCGACGCGCGCGGGCTGCGCGAGGCGATCGCCACGGCCGCGCGCAGCAAGCCCTTCCTC
GGCATCTGCATGGGCATGCAGGTGCTCTTCGAGCACAGCGAGGAAGGCGATACGGCCTGCCTCGGGATTCTTCCTGGCAA
CGTGCAGCGCTTTCCGCACGAGGCTATGCACGACGATCGCGGCAACAAGCTCAAGGTCCCGCACATGGGCTGGAACAACG
TCCACCAGGGCGCGCCGCATCCGCTGTGGTCCGGAATCGAGGACGGCGAACGCTTTTACTTCGTTCACAGCTATTTCGTG
CAGCCGACGACGCCCGACTTGATCGCGGGCTTCTCGCATTACCCCTTTCCGTTCACCTGCGCCGTCGCGTCCGGAAACAT
CTTCGCCGTCCAGTTCCACCCGGAAAAAAGCCAGAACGCCGGGCTCACGCTGCTCGGAAACTTCGTGACCTGGAATCCGG
GCGAGCACGCCTCGGCCTGCGATCTCTCCGGCGCAAGCTGCGCCTGA

Upstream 100 bases:

>100_bases
CGGCTGGGCGACGTGACCCCTTCGACCAAAGGTGCGCTGTGACGATGGTCCGCTTCGCCTGCTGTGTTCAAGCGCGACCT
TCCTCAGCGGGAGCCGGAGC

Downstream 100 bases:

>100_bases
CTCTTCTCACCTTCTGCTCGACCTGTTGACCAGCCATGTTAATCATTCCCGCAATCGACCTTAAAGACGGACACTGCGTG
CGCCTCGAACAGGGCGAAAT

Product: imidazole glycerol phosphate synthase subunit hisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSVDIAVVDYGMGNLRSVSKAIEHVAPSATVVVTSSPDVIAEAGRVVFPGQGAARDCMREIDARGLREAIATAARSKPFL
GICMGMQVLFEHSEEGDTACLGILPGNVQRFPHEAMHDDRGNKLKVPHMGWNNVHQGAPHPLWSGIEDGERFYFVHSYFV
QPTTPDLIAGFSHYPFPFTCAVASGNIFAVQFHPEKSQNAGLTLLGNFVTWNPGEHASACDLSGASCA

Sequences:

>Translated_228_residues
MSVDIAVVDYGMGNLRSVSKAIEHVAPSATVVVTSSPDVIAEAGRVVFPGQGAARDCMREIDARGLREAIATAARSKPFL
GICMGMQVLFEHSEEGDTACLGILPGNVQRFPHEAMHDDRGNKLKVPHMGWNNVHQGAPHPLWSGIEDGERFYFVHSYFV
QPTTPDLIAGFSHYPFPFTCAVASGNIFAVQFHPEKSQNAGLTLLGNFVTWNPGEHASACDLSGASCA
>Mature_227_residues
SVDIAVVDYGMGNLRSVSKAIEHVAPSATVVVTSSPDVIAEAGRVVFPGQGAARDCMREIDARGLREAIATAARSKPFLG
ICMGMQVLFEHSEEGDTACLGILPGNVQRFPHEAMHDDRGNKLKVPHMGWNNVHQGAPHPLWSGIEDGERFYFVHSYFVQ
PTTPDLIAGFSHYPFPFTCAVASGNIFAVQFHPEKSQNAGLTLLGNFVTWNPGEHASACDLSGASCA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=41.3461538461538, Blast_Score=134, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=32.8703703703704, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_THIDA (Q3SEU6)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315468.1
- ProteinModelPortal:   Q3SEU6
- SMR:   Q3SEU6
- STRING:   Q3SEU6
- GeneID:   3671293
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1710
- NMPDR:   fig|292415.3.peg.1807
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- PhylomeDB:   Q3SEU6
- BioCyc:   TDEN292415:TBD_1710-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 24508; Mature: 24377

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 83-83 ACT_SITE 193-193 ACT_SITE 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDIAVVDYGMGNLRSVSKAIEHVAPSATVVVTSSPDVIAEAGRVVFPGQGAARDCMRE
CCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHCCCEEECCCCHHHHHHHH
IDARGLREAIATAARSKPFLGICMGMQVLFEHSEEGDTACLGILPGNVQRFPHEAMHDDR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCCHHHHCCCC
GNKLKVPHMGWNNVHQGAPHPLWSGIEDGERFYFVHSYFVQPTTPDLIAGFSHYPFPFTC
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHCCCCCCCCEEE
AVASGNIFAVQFHPEKSQNAGLTLLGNFVTWNPGEHASACDLSGASCA
EEECCCEEEEEECCCCCCCCCEEEEECEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVDIAVVDYGMGNLRSVSKAIEHVAPSATVVVTSSPDVIAEAGRVVFPGQGAARDCMRE
CEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHCCCEEECCCCHHHHHHHH
IDARGLREAIATAARSKPFLGICMGMQVLFEHSEEGDTACLGILPGNVQRFPHEAMHDDR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCCHHHHCCCC
GNKLKVPHMGWNNVHQGAPHPLWSGIEDGERFYFVHSYFVQPTTPDLIAGFSHYPFPFTC
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHCCCCCCCCEEE
AVASGNIFAVQFHPEKSQNAGLTLLGNFVTWNPGEHASACDLSGASCA
EEECCCEEEEEECCCCCCCCCEEEEECEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA