Click here to switch to the map view.

The map label for this gene is hisA

Identifier: 74317727

GI number: 74317727

Start: 1797312

End: 1798067

Strand: Reverse

Name: hisA

Synonym: Tbd_1709

Alternate gene names: 74317727

Gene position: 1798067-1797312 (Counterclockwise)

Preceding gene: 74317728

Following gene: 74317726

Centisome position: 61.79

GC content: 66.01

Gene sequence:

>756_bases
ATGTTAATCATTCCCGCAATCGACCTTAAAGACGGACACTGCGTGCGCCTCGAACAGGGCGAAATGGACAAGGCGACCGT
GTTCTCGGAGGACCCGGCCGCGATGGCGCGGCGTTGGAAGGAGCATGGCGCGCGGCGCCTGCATCTCGTCGATCTCAACG
GCGCCTTCGCCGGAAAGCCCGTCAACGATGCTGCGATCCAGGCCATCGTCGAGGCCGTGGGCGACGAGATGCCGGTACAA
CTCGGCGGTGGCATCCGCGACCTCGCGACGATCGAGCGCTATCTCGACGACGGCGTGCGCTACGTCATCATCGGCACCGC
CGCCGTGAAGAATCCCGGCTTCCTGCATGAGGCCTGCGATGCCTTCCCCGGCCACGTGATGGTCGGGCTCGACGCCCGTG
ACGGCAAGGTCGCGGTCGAGGGCTGGTCCAAGCTCACCGGCCATGACGTCGTCGATCTCGCCAAGCGTTTCGAGGGCTAC
GGCGTCGAGGCGGTCATTTACACCGACATCGGTCGCGACGGCATGCTGACGGGCGTCAACATCGACGCGACACAACGTCT
GGCCCAGGCGCTGTCGATTCCGGTCATCGCGAGCGGCGGCGTGACCAATCTCGACGACGTGCGCGGACTGTCGGCGGTCG
CCAAGGACGGTATCATCGGCGCGATCACCGGGCGCGCGATCTATCAGGGCACGCTCGACTTCGCCGAAGCGCAGAAGCTC
GCCGACGAACTCGCGGGCGGCGTGTTCGGCGTCTGA

Upstream 100 bases:

>100_bases
TCGTGACCTGGAATCCGGGCGAGCACGCCTCGGCCTGCGATCTCTCCGGCGCAAGCTGCGCCTGACTCTTCTCACCTTCT
GCTCGACCTGTTGACCAGCC

Downstream 100 bases:

>100_bases
TTCGATGCTCGCCAAACGGATCATCCCCTGCCTGGACGTGACCAACGGTCGCGTCGTCAAGGGCGTCAACTTCGTCGAAT
TGAAGGACGCCGGCGACCCG

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLIIPAIDLKDGHCVRLEQGEMDKATVFSEDPAAMARRWKEHGARRLHLVDLNGAFAGKPVNDAAIQAIVEAVGDEMPVQ
LGGGIRDLATIERYLDDGVRYVIIGTAAVKNPGFLHEACDAFPGHVMVGLDARDGKVAVEGWSKLTGHDVVDLAKRFEGY
GVEAVIYTDIGRDGMLTGVNIDATQRLAQALSIPVIASGGVTNLDDVRGLSAVAKDGIIGAITGRAIYQGTLDFAEAQKL
ADELAGGVFGV

Sequences:

>Translated_251_residues
MLIIPAIDLKDGHCVRLEQGEMDKATVFSEDPAAMARRWKEHGARRLHLVDLNGAFAGKPVNDAAIQAIVEAVGDEMPVQ
LGGGIRDLATIERYLDDGVRYVIIGTAAVKNPGFLHEACDAFPGHVMVGLDARDGKVAVEGWSKLTGHDVVDLAKRFEGY
GVEAVIYTDIGRDGMLTGVNIDATQRLAQALSIPVIASGGVTNLDDVRGLSAVAKDGIIGAITGRAIYQGTLDFAEAQKL
ADELAGGVFGV
>Mature_251_residues
MLIIPAIDLKDGHCVRLEQGEMDKATVFSEDPAAMARRWKEHGARRLHLVDLNGAFAGKPVNDAAIQAIVEAVGDEMPVQ
LGGGIRDLATIERYLDDGVRYVIIGTAAVKNPGFLHEACDAFPGHVMVGLDARDGKVAVEGWSKLTGHDVVDLAKRFEGY
GVEAVIYTDIGRDGMLTGVNIDATQRLAQALSIPVIASGGVTNLDDVRGLSAVAKDGIIGAITGRAIYQGTLDFAEAQKL
ADELAGGVFGV

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=34.5679012345679, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788336, Length=245, Percent_Identity=22.4489795918367, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_THIDA (Q3SEU7)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315467.1
- ProteinModelPortal:   Q3SEU7
- SMR:   Q3SEU7
- STRING:   Q3SEU7
- GeneID:   3671292
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1709
- NMPDR:   fig|292415.3.peg.1806
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- PhylomeDB:   Q3SEU7
- ProtClustDB:   PRK00748
- BioCyc:   TDEN292415:TBD_1709-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26504; Mature: 26504

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIPAIDLKDGHCVRLEQGEMDKATVFSEDPAAMARRWKEHGARRLHLVDLNGAFAGKP
CEEEEEEECCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCC
VNDAAIQAIVEAVGDEMPVQLGGGIRDLATIERYLDDGVRYVIIGTAAVKNPGFLHEACD
CCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHH
AFPGHVMVGLDARDGKVAVEGWSKLTGHDVVDLAKRFEGYGVEAVIYTDIGRDGMLTGVN
CCCCEEEEEEECCCCEEEECCCHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEECC
IDATQRLAQALSIPVIASGGVTNLDDVRGLSAVAKDGIIGAITGRAIYQGTLDFAEAQKL
CHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCEEEEEECCEEEECCCCHHHHHHH
ADELAGGVFGV
HHHHCCCCCCC
>Mature Secondary Structure
MLIIPAIDLKDGHCVRLEQGEMDKATVFSEDPAAMARRWKEHGARRLHLVDLNGAFAGKP
CEEEEEEECCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCC
VNDAAIQAIVEAVGDEMPVQLGGGIRDLATIERYLDDGVRYVIIGTAAVKNPGFLHEACD
CCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHH
AFPGHVMVGLDARDGKVAVEGWSKLTGHDVVDLAKRFEGYGVEAVIYTDIGRDGMLTGVN
CCCCEEEEEEECCCCEEEECCCHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEECC
IDATQRLAQALSIPVIASGGVTNLDDVRGLSAVAKDGIIGAITGRAIYQGTLDFAEAQKL
CHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCEEEEEECCEEEECCCCHHHHHHH
ADELAGGVFGV
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA