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The map label for this gene is hisF

Identifier: 74317726

GI number: 74317726

Start: 1796552

End: 1797307

Strand: Reverse

Name: hisF

Synonym: Tbd_1708

Alternate gene names: 74317726

Gene position: 1797307-1796552 (Counterclockwise)

Preceding gene: 74317727

Following gene: 74317725

Centisome position: 61.77

GC content: 65.87

Gene sequence:

>756_bases
ATGCTCGCCAAACGGATCATCCCCTGCCTGGACGTGACCAACGGTCGCGTCGTCAAGGGCGTCAACTTCGTCGAATTGAA
GGACGCCGGCGACCCGGTCGAAATCGCGCGCGGCTACAACGAGGCCGGCGCCGACGAACTGACCTTTCTCGACATCACCG
CGTCGAGCGACAACCGCGACCTGATCCTGCACATCGTCGAAGCCGTCGCGTCCGAGGTATTCATTCCACTGACGGTCGGC
GGCGGCGTGCGGGCGGTCGCCGACGTGCAGCGACTGCTCAACGCCGGTGCCGACAAGGTCAGCATCAACACCTCGGCGGT
CACCAACCCCCAGCTCGTCAAGGACGCCGCCGACCGCTACGGCAGCCAATGCATCGTCGTCGCGATCGACGCCAAGCGCG
TGGGTGACCATTGGGAGGTCTTCACCCACGGCGGGCGTACGGCGACCGGCCTCGACGCGGTCGAGTGGGCGAAGAAGATG
GAAAGCCTCGGCGCGGGCGAGTTGCTGCTGACCTCGATGGACCGCGACGGCACCAAGACCGGCTTCGACCTCGAATTGAC
GCGCGCGATTTCCGACGCGGTCGACGTTCCGATCGTCGCCAGCGGGGGTGTCGGAACGCTGCAACACATGGTCGACGGCG
TGCGCGAAGGCCGGGCCGATGCCGTCCTCGCCGCGAGCATTTTTCATTTCGGCGAATACCGGGTCGACGAGGCCAAGGCC
TACATGAAGCGGCACGGAATCGAGGTGCGCCTGTGA

Upstream 100 bases:

>100_bases
GCGATCACCGGGCGCGCGATCTATCAGGGCACGCTCGACTTCGCCGAAGCGCAGAAGCTCGCCGACGAACTCGCGGGCGG
CGTGTTCGGCGTCTGATTCG

Downstream 100 bases:

>100_bases
GCGACTGGCTCGACGCGGTGAAATGGGACGCCCAGGGCCTCGTCCCGGCGATCGCCCAGGATGCGGCGAGCGGGGAAATC
CTCATGGTGGCGTGGATGAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLAKRIIPCLDVTNGRVVKGVNFVELKDAGDPVEIARGYNEAGADELTFLDITASSDNRDLILHIVEAVASEVFIPLTVG
GGVRAVADVQRLLNAGADKVSINTSAVTNPQLVKDAADRYGSQCIVVAIDAKRVGDHWEVFTHGGRTATGLDAVEWAKKM
ESLGAGELLLTSMDRDGTKTGFDLELTRAISDAVDVPIVASGGVGTLQHMVDGVREGRADAVLAASIFHFGEYRVDEAKA
YMKRHGIEVRL

Sequences:

>Translated_251_residues
MLAKRIIPCLDVTNGRVVKGVNFVELKDAGDPVEIARGYNEAGADELTFLDITASSDNRDLILHIVEAVASEVFIPLTVG
GGVRAVADVQRLLNAGADKVSINTSAVTNPQLVKDAADRYGSQCIVVAIDAKRVGDHWEVFTHGGRTATGLDAVEWAKKM
ESLGAGELLLTSMDRDGTKTGFDLELTRAISDAVDVPIVASGGVGTLQHMVDGVREGRADAVLAASIFHFGEYRVDEAKA
YMKRHGIEVRL
>Mature_251_residues
MLAKRIIPCLDVTNGRVVKGVNFVELKDAGDPVEIARGYNEAGADELTFLDITASSDNRDLILHIVEAVASEVFIPLTVG
GGVRAVADVQRLLNAGADKVSINTSAVTNPQLVKDAADRYGSQCIVVAIDAKRVGDHWEVFTHGGRTATGLDAVEWAKKM
ESLGAGELLLTSMDRDGTKTGFDLELTRAISDAVDVPIVASGGVGTLQHMVDGVREGRADAVLAASIFHFGEYRVDEAKA
YMKRHGIEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=43.579766536965, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=28.2786885245902, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.7133757961783, Blast_Score=173, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_THIDA (Q3SEU8)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315466.1
- ProteinModelPortal:   Q3SEU8
- SMR:   Q3SEU8
- STRING:   Q3SEU8
- GeneID:   3671291
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1708
- NMPDR:   fig|292415.3.peg.1805
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q3SEU8
- BioCyc:   TDEN292415:TBD_1708-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26800; Mature: 26800

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVTNGRVVKGVNFVELKDAGDPVEIARGYNEAGADELTFLDITASSDNRD
CCHHHCCCCEECCCCEEEECCEEEEECCCCCHHHHHCCCCCCCCCCEEEEEEEECCCCCH
LILHIVEAVASEVFIPLTVGGGVRAVADVQRLLNAGADKVSINTSAVTNPQLVKDAADRY
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHC
GSQCIVVAIDAKRVGDHWEVFTHGGRTATGLDAVEWAKKMESLGAGELLLTSMDRDGTKT
CCEEEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCC
GFDLELTRAISDAVDVPIVASGGVGTLQHMVDGVREGRADAVLAASIFHFGEYRVDEAKA
CCCEEHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
YMKRHGIEVRL
HHHHCCCEEEC
>Mature Secondary Structure
MLAKRIIPCLDVTNGRVVKGVNFVELKDAGDPVEIARGYNEAGADELTFLDITASSDNRD
CCHHHCCCCEECCCCEEEECCEEEEECCCCCHHHHHCCCCCCCCCCEEEEEEEECCCCCH
LILHIVEAVASEVFIPLTVGGGVRAVADVQRLLNAGADKVSINTSAVTNPQLVKDAADRY
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHC
GSQCIVVAIDAKRVGDHWEVFTHGGRTATGLDAVEWAKKMESLGAGELLLTSMDRDGTKT
CCEEEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCC
GFDLELTRAISDAVDVPIVASGGVGTLQHMVDGVREGRADAVLAASIFHFGEYRVDEAKA
CCCEEHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
YMKRHGIEVRL
HHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA