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The map label for this gene is gph [H]

Identifier: 74317580

GI number: 74317580

Start: 1644183

End: 1644839

Strand: Reverse

Name: gph [H]

Synonym: Tbd_1562

Alternate gene names: 74317580

Gene position: 1644839-1644183 (Counterclockwise)

Preceding gene: 74317581

Following gene: 74317579

Centisome position: 56.53

GC content: 68.49

Gene sequence:

>657_bases
ATGCCCAAGCAATTTGACCTGCTGATTTTCGACTGGGACGGCACCTTGATGGACTCGGCCGGCGTGATCGTCGATTCGAT
CCAGCGTGCCTGCGTCGATATCGGCCTGCCGACGCCGACCGAGCACGCCTCGCGCCAGATCATCGGCCTTGGTCTCGTTC
AGGCGCTGCAGGCGCTCGTTCCCGATCTGCCCGCCGACGACTACCCGCGTCTGGTTGAGCGCTACCGCAAGCATTACCTC
GGCCGCGACGCCGCGATCCCGCTGTTCCCGGGCGTGGTCGACGGCATTCGCGCGCTGCACTCGAGTGGCTTCCAGCTCGC
GGTTGCGACCGGCAAGAGCCACGTCGGCCTTTCGCGCGCACTCGACACGAGCGGGCTGCGGCCGTGGTTCTGCGCGACGC
GCTGCGCCGACCAGACGCACTCGAAGCCGCATCCGGCGATGGTCCTCGAACTGATTGACGAACTCGGCGCCGACCCGGCG
CGCACGCTCGTCATCGGCGACACGAGCCACGACCTCCTGATGGCCGCGAACGCCGGCGTCGCGAGCCTCGGCGTGACCTA
CGGCGCGCACGAGGCCGCGAGCCTCCACCCGCATGCGCCGGCGGCGTTGCTCGACAGTTTCGACGAGGTGTACGCATGGC
TCGACGCGAACGCCTGA

Upstream 100 bases:

>100_bases
GCCGCCAAGCTCGCGTTCGCCCACCCGATCTCGGGCGAGCGCATGACGATCGAGGCGCCTTTACCCGCCGACCTTGCGAC
CTTTCTGGACACGCTCGACC

Downstream 100 bases:

>100_bases
TCTGCCGCGCCGAAGACCTGGCCGAGGCGGGGCAGGGCGTCCGTTTCGACGTGCAGCGCGGTGACCTTCGCCGGCCCGCC
TTCGTCGTCCGCTTCGAGGG

Product: putative hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPKQFDLLIFDWDGTLMDSAGVIVDSIQRACVDIGLPTPTEHASRQIIGLGLVQALQALVPDLPADDYPRLVERYRKHYL
GRDAAIPLFPGVVDGIRALHSSGFQLAVATGKSHVGLSRALDTSGLRPWFCATRCADQTHSKPHPAMVLELIDELGADPA
RTLVIGDTSHDLLMAANAGVASLGVTYGAHEAASLHPHAPAALLDSFDEVYAWLDANA

Sequences:

>Translated_218_residues
MPKQFDLLIFDWDGTLMDSAGVIVDSIQRACVDIGLPTPTEHASRQIIGLGLVQALQALVPDLPADDYPRLVERYRKHYL
GRDAAIPLFPGVVDGIRALHSSGFQLAVATGKSHVGLSRALDTSGLRPWFCATRCADQTHSKPHPAMVLELIDELGADPA
RTLVIGDTSHDLLMAANAGVASLGVTYGAHEAASLHPHAPAALLDSFDEVYAWLDANA
>Mature_217_residues
PKQFDLLIFDWDGTLMDSAGVIVDSIQRACVDIGLPTPTEHASRQIIGLGLVQALQALVPDLPADDYPRLVERYRKHYLG
RDAAIPLFPGVVDGIRALHSSGFQLAVATGKSHVGLSRALDTSGLRPWFCATRCADQTHSKPHPAMVLELIDELGADPAR
TLVIGDTSHDLLMAANAGVASLGVTYGAHEAASLHPHAPAALLDSFDEVYAWLDANA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=224, Percent_Identity=26.7857142857143, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23306; Mature: 23175

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQFDLLIFDWDGTLMDSAGVIVDSIQRACVDIGLPTPTEHASRQIIGLGLVQALQALV
CCCCEEEEEECCCCCEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
PDLPADDYPRLVERYRKHYLGRDAAIPLFPGVVDGIRALHSSGFQLAVATGKSHVGLSRA
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
LDTSGLRPWFCATRCADQTHSKPHPAMVLELIDELGADPARTLVIGDTSHDLLMAANAGV
HCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCH
ASLGVTYGAHEAASLHPHAPAALLDSFDEVYAWLDANA
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PKQFDLLIFDWDGTLMDSAGVIVDSIQRACVDIGLPTPTEHASRQIIGLGLVQALQALV
CCCEEEEEECCCCCEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
PDLPADDYPRLVERYRKHYLGRDAAIPLFPGVVDGIRALHSSGFQLAVATGKSHVGLSRA
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
LDTSGLRPWFCATRCADQTHSKPHPAMVLELIDELGADPARTLVIGDTSHDLLMAANAGV
HCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCH
ASLGVTYGAHEAASLHPHAPAALLDSFDEVYAWLDANA
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]