Click here to switch to the map view.

The map label for this gene is grpE

Identifier: 74317555

GI number: 74317555

Start: 1621818

End: 1622339

Strand: Direct

Name: grpE

Synonym: Tbd_1537

Alternate gene names: 74317555

Gene position: 1621818-1622339 (Clockwise)

Preceding gene: 74317551

Following gene: 74317556

Centisome position: 55.74

GC content: 65.13

Gene sequence:

>522_bases
ATGCAGGACGAATTCAAGACCGATACCCCACGCACCGAAGCCGGGTCTGAGAAGGAAACCATGCCGAGCATGGAAGAGCT
GCTCAAGGCAGCCGAACTCGCCGCGGCCGAACACCACGACGCCTGGCTGCGCGCCAAGGCAGAGACCGAGAACATGCGGC
GCCGCGCGGCGGAAGACGTCGACAAGGCCCGCAAATTCGCCGTCGAGAGCTTCGCCGGCGAACTCCTGGCGGTGAAGGAC
AGCCTCGAAGCCGCGCTCGCCGCCGAGTCGCCGAGCGTGGACAACCTGAAGGACGGCGTGACGCTGACGCTGAAGCAACT
GAGTGCAGTCTTCGGCAAGTTCAACCTCCACGACATCGAGCCGCTGGGCGAAAAATTCGACCCCCACCTGCATCAGGCGA
TCCAGGTCGTCGAGTCGGAGCAGCCGGCGAACACCGTGGTGACGGTGCTGCAGAAGGGCTACCGGCTCCATGACCGCACG
CTGCGGCCGGCGCTGGTCATGGTCGCCAAGGGGAAGGACTGA

Upstream 100 bases:

>100_bases
CGGGCAAACCGCGCCGCGCGGACGCGAGAAAAACCCGCGCCGGCCTCTTGAAGCCTTGGCGTTCGTCCCCATCTGCCCTC
GAGGACCACGAGGAGCAAGC

Downstream 100 bases:

>100_bases
ACGCGGAGCCTTGAAATGGGCGCGCTGCGCCCCATATTCGGATCAAATTGAATCTCTTTTAGAAGGAAAAAATCATGGGA
CGCATCATTGGTATTGACCT

Product: molecular chaperone protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MQDEFKTDTPRTEAGSEKETMPSMEELLKAAELAAAEHHDAWLRAKAETENMRRRAAEDVDKARKFAVESFAGELLAVKD
SLEAALAAESPSVDNLKDGVTLTLKQLSAVFGKFNLHDIEPLGEKFDPHLHQAIQVVESEQPANTVVTVLQKGYRLHDRT
LRPALVMVAKGKD

Sequences:

>Translated_173_residues
MQDEFKTDTPRTEAGSEKETMPSMEELLKAAELAAAEHHDAWLRAKAETENMRRRAAEDVDKARKFAVESFAGELLAVKD
SLEAALAAESPSVDNLKDGVTLTLKQLSAVFGKFNLHDIEPLGEKFDPHLHQAIQVVESEQPANTVVTVLQKGYRLHDRT
LRPALVMVAKGKD
>Mature_173_residues
MQDEFKTDTPRTEAGSEKETMPSMEELLKAAELAAAEHHDAWLRAKAETENMRRRAAEDVDKARKFAVESFAGELLAVKD
SLEAALAAESPSVDNLKDGVTLTLKQLSAVFGKFNLHDIEPLGEKFDPHLHQAIQVVESEQPANTVVTVLQKGYRLHDRT
LRPALVMVAKGKD

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=179, Percent_Identity=38.5474860335196, Blast_Score=104, Evalue=3e-23,
Organism=Homo sapiens, GI40255109, Length=138, Percent_Identity=38.4057971014493, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1788967, Length=149, Percent_Identity=45.6375838926174, Blast_Score=112, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17552458, Length=172, Percent_Identity=34.8837209302326, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324806, Length=165, Percent_Identity=32.1212121212121, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653432, Length=181, Percent_Identity=37.5690607734807, Blast_Score=103, Evalue=7e-23,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_THIDA (Q3SIN5)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315295.1
- ProteinModelPortal:   Q3SIN5
- SMR:   Q3SIN5
- STRING:   Q3SIN5
- GeneID:   3672017
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1537
- NMPDR:   fig|292415.3.peg.1537
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   HEAIAYV
- PhylomeDB:   Q3SIN5
- BioCyc:   TDEN292415:TBD_1537-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 19147; Mature: 19147

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDEFKTDTPRTEAGSEKETMPSMEELLKAAELAAAEHHDAWLRAKAETENMRRRAAEDV
CCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKARKFAVESFAGELLAVKDSLEAALAAESPSVDNLKDGVTLTLKQLSAVFGKFNLHDIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCCC
PLGEKFDPHLHQAIQVVESEQPANTVVTVLQKGYRLHDRTLRPALVMVAKGKD
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCCEEEEECCCC
>Mature Secondary Structure
MQDEFKTDTPRTEAGSEKETMPSMEELLKAAELAAAEHHDAWLRAKAETENMRRRAAEDV
CCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKARKFAVESFAGELLAVKDSLEAALAAESPSVDNLKDGVTLTLKQLSAVFGKFNLHDIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCCC
PLGEKFDPHLHQAIQVVESEQPANTVVTVLQKGYRLHDRTLRPALVMVAKGKD
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA