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The map label for this gene is miaA

Identifier: 74317530

GI number: 74317530

Start: 1600548

End: 1601489

Strand: Reverse

Name: miaA

Synonym: Tbd_1512

Alternate gene names: 74317530

Gene position: 1601489-1600548 (Counterclockwise)

Preceding gene: 74317533

Following gene: 74317528

Centisome position: 55.04

GC content: 69.85

Gene sequence:

>942_bases
ATGTCCACACTCGGCCTTCCTCCTGCCGTTTTCCTCATGGGACCGACCGCTTCCGGCAAGACGGCGCTGGCGGTCAGTCT
CCGTTCACGGTTTCCGTTCGAAATCATCAGCGTCGATTCGGCCCTGGTCTACCGGGGCATGGACGTCGGCACGGCGAAGC
CCGACGCGGCGACGCTCGCGCGCGCACCGCATCATCTGCTCGATATCCGTGAGCCGAACGAAACGTATTCGGCGGCGGCG
TTCTGCGACGACGCGCGCGCGCGGATGGCGGCGATCACCGCGCGGGGCCGGGTGCCGCTGCTCGTCGGCGGCACGATGCT
GTACTTCCGTGCCCTGCTGCAGGGACTCGACGACCTGCCGCGCGCCGATGCCGCGCTCCGCAAGCGGCTCGAGCAGGATG
CCGCCGAACGCGGCTGGCCGGCGCTCCACGCCGAACTCGCCGCGCTCGACCCGGCGACCGCGGCGCGCCTTGCCCCCAAC
GATTCCCAGCGCATCGGGCGGGCGCTCGAGATCGTCGAGCTGACCGGACGACCGATGTCGGCGCAGCTCGCCCAAACCCC
CCGCGCATTGCCCTACGACGTGCTGCAACTGGCCCTGGTCCCGTCCGACCGCGCCGCGCTGCACCGGCGCATTGGCGAGC
GCTTCGACGCCATGCTCGAGGCCGGCCTTGTCGACGAAGTGGAAATGCTTCGCCGCAATCACGTGCTCGACTCGACCATG
CCCGCGATGCGTGCCGTGGGCTATCGCCAAGCGTGGGCGTATCTCGACGGCGACATTGATCTGGAGGCCTTGCGCGAACA
AGGTGTCGCCGCGACCCGCCAACTCGCCAAGCGCCAATTGACCTGGCTGCGTTCATGGCCGGGGGCGGTGACGCTCGACT
GCCTGGCGGACGACCTCGAGTCCCGGGCCGCAGCTCTCGTCGCTGCCCACCTCGGCGCCTGA

Upstream 100 bases:

>100_bases
GTGCTCTTGAGACGGGGCGGCGCTTTCCTTCCAACTCTAGACCGTAATTCGCTCCCGAAAGACCTGCGCGCGGCGCGGAC
GGCTGCGCGATAATCCCGGG

Downstream 100 bases:

>100_bases
GTCGCCTATTCGGGCTGCGCCGACTCGACCCGCGCGTGCGCCGCGCCGCGATGGAAACGGATCTCGAGCCCGTCACCGGC
GCGCAGCGTCGCGGCGTCGT

Product: tRNA isopentenyltransferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSTLGLPPAVFLMGPTASGKTALAVSLRSRFPFEIISVDSALVYRGMDVGTAKPDAATLARAPHHLLDIREPNETYSAAA
FCDDARARMAAITARGRVPLLVGGTMLYFRALLQGLDDLPRADAALRKRLEQDAAERGWPALHAELAALDPATAARLAPN
DSQRIGRALEIVELTGRPMSAQLAQTPRALPYDVLQLALVPSDRAALHRRIGERFDAMLEAGLVDEVEMLRRNHVLDSTM
PAMRAVGYRQAWAYLDGDIDLEALREQGVAATRQLAKRQLTWLRSWPGAVTLDCLADDLESRAAALVAAHLGA

Sequences:

>Translated_313_residues
MSTLGLPPAVFLMGPTASGKTALAVSLRSRFPFEIISVDSALVYRGMDVGTAKPDAATLARAPHHLLDIREPNETYSAAA
FCDDARARMAAITARGRVPLLVGGTMLYFRALLQGLDDLPRADAALRKRLEQDAAERGWPALHAELAALDPATAARLAPN
DSQRIGRALEIVELTGRPMSAQLAQTPRALPYDVLQLALVPSDRAALHRRIGERFDAMLEAGLVDEVEMLRRNHVLDSTM
PAMRAVGYRQAWAYLDGDIDLEALREQGVAATRQLAKRQLTWLRSWPGAVTLDCLADDLESRAAALVAAHLGA
>Mature_312_residues
STLGLPPAVFLMGPTASGKTALAVSLRSRFPFEIISVDSALVYRGMDVGTAKPDAATLARAPHHLLDIREPNETYSAAAF
CDDARARMAAITARGRVPLLVGGTMLYFRALLQGLDDLPRADAALRKRLEQDAAERGWPALHAELAALDPATAARLAPND
SQRIGRALEIVELTGRPMSAQLAQTPRALPYDVLQLALVPSDRAALHRRIGERFDAMLEAGLVDEVEMLRRNHVLDSTMP
AMRAVGYRQAWAYLDGDIDLEALREQGVAATRQLAKRQLTWLRSWPGAVTLDCLADDLESRAAALVAAHLGA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=320, Percent_Identity=29.375, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1790613, Length=294, Percent_Identity=55.7823129251701, Blast_Score=331, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI25144712, Length=273, Percent_Identity=26.007326007326, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324848, Length=290, Percent_Identity=29.6551724137931, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24649830, Length=331, Percent_Identity=29.0030211480363, Blast_Score=125, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_THIDA (Q3SIR0)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315270.1
- ProteinModelPortal:   Q3SIR0
- SMR:   Q3SIR0
- STRING:   Q3SIR0
- GeneID:   3672925
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1512
- NMPDR:   fig|292415.3.peg.1512
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   DAISHER
- PhylomeDB:   Q3SIR0
- BioCyc:   TDEN292415:TBD_1512-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33899; Mature: 33768

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLGLPPAVFLMGPTASGKTALAVSLRSRFPFEIISVDSALVYRGMDVGTAKPDAATLA
CCCCCCCCEEEEECCCCCCCEEEEEEHHCCCCEEEEECCHHHHHCCCCCCCCCCCHHHHH
RAPHHLLDIREPNETYSAAAFCDDARARMAAITARGRVPLLVGGTMLYFRALLQGLDDLP
HCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCC
RADAALRKRLEQDAAERGWPALHAELAALDPATAARLAPNDSQRIGRALEIVELTGRPMS
CHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCH
AQLAQTPRALPYDVLQLALVPSDRAALHRRIGERFDAMLEAGLVDEVEMLRRNHVLDSTM
HHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
PAMRAVGYRQAWAYLDGDIDLEALREQGVAATRQLAKRQLTWLRSWPGAVTLDCLADDLE
HHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
SRAAALVAAHLGA
HHHHHHHHHHHCC
>Mature Secondary Structure 
STLGLPPAVFLMGPTASGKTALAVSLRSRFPFEIISVDSALVYRGMDVGTAKPDAATLA
CCCCCCCEEEEECCCCCCCEEEEEEHHCCCCEEEEECCHHHHHCCCCCCCCCCCHHHHH
RAPHHLLDIREPNETYSAAAFCDDARARMAAITARGRVPLLVGGTMLYFRALLQGLDDLP
HCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCC
RADAALRKRLEQDAAERGWPALHAELAALDPATAARLAPNDSQRIGRALEIVELTGRPMS
CHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCH
AQLAQTPRALPYDVLQLALVPSDRAALHRRIGERFDAMLEAGLVDEVEMLRRNHVLDSTM
HHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
PAMRAVGYRQAWAYLDGDIDLEALREQGVAATRQLAKRQLTWLRSWPGAVTLDCLADDLE
HHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
SRAAALVAAHLGA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA