BacMap

Definition	Thiobacillus denitrificans ATCC 25259 chromosome, complete genome.
Accession	NC_007404
Length	2,909,809

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The map label for this gene is cbpA [H]

Identifier: 74317449

GI number: 74317449

Start: 1519338

End: 1520294

Strand: Reverse

Name: cbpA [H]

Synonym: Tbd_1431

Alternate gene names: 74317449

Gene position: 1520294-1519338 (Counterclockwise)

Preceding gene: 74317463

Following gene: 74317448

Centisome position: 52.25

GC content: 68.03

Gene sequence:

>957_bases
ATGGAATTCAAGGACTACTACAAGATCTTGGGCGTGCCGCGCGACGCCACGGCGGACGACATCAAGAAGGCCTTCCGCAA
GCTCGCGCGCAAGTATCACCCCGACATCTCGAAGGAAGCCGGCGCCGAAACGCGGATGCAGGAGATCAACGAAGCGAACG
CGGTGCTGTCCGACCCCGAGAAGCGCGCCGCCTACGACCAGCTTGGCAAGGGCTACCAGCCCGGACAGGAATTCCGCCCG
CCGCCCGATTGGGACGCGGGCTTCGAATTCTCGGGCCGCGGTTTCGGCGGCCAGGAGACCGCAGATTTTTCCGATTTTTT
CGCCGAACTGTTCGGCGGGCTCGGAGGCGGGCGCGGCAGTCCTTTTCACACCCGCGGCGGACGCGCGGGCTACCAGGCGC
GCGGCGAGGATCATCACGCCAAGGTCGTGCTCGATCTCGCCGACGCGTTCACCGGGGCGACGCGGCAGGTTTCGTTGCGC
GCGCCCGAGGTCGACCCGCAGGGGCACGTCGTGCTCAAGACCCGCACGCTCAACGTCAAGATCCCGAAGGGCGTGCGCGA
AGGTCAGGTCATCCGCCTTGCGGGGCAAGGAGCGCCAGGAGCCGGCGGGGCGGCGGCCGGCGATCTATTGCTCGAGATCC
ACTTCAAACAGGATTCGCGATTGCGCGCGGTCGGGCGGGATCTCTACCTGACGCTGCCGGTCGCGCCGTGGGAGGCGGCG
CTCGGCGCGGTCGTCGCGGTCGAGCTCCCGGGCGGGGCGGTCAAGGTCAGGATTCCCGAAGGCGCCCAGAGCGGCAAGCA
GCTGCGGGTGCGCGGCAAAGGCATACCGGGCGAGCCGCCCGGCGACCTGCTGCTCGACCTTCGCGTCGTACTGCCGCCCG
CCGACACGCCGCAGGCCAAGGCATTTTATGAATCCATGGCGCGCGAGTTTGCCTTCGATCCGCGCGGGAGGAACTGA

Upstream 100 bases:

>100_bases
GAGGCGATCGTTCATACCTGGGTCGGCAGCAGCGCACCGCCCTGCGAACGCCCTCGTACCCGCACCCGTACCCGTACCCG
TACCTGGACAAGGACTGCTC

Downstream 100 bases:

>100_bases
TCGTGAGCGAGAACGAAGTCTTCGTTGGCGTCCTGATGGACGATTGCTGGCTGACCGTGGAACAGGTCGCCGCGGCCTGT
GTGGTTGAGCCCGCGTGGCT

Product: heat shock protein DnaJ family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MEFKDYYKILGVPRDATADDIKKAFRKLARKYHPDISKEAGAETRMQEINEANAVLSDPEKRAAYDQLGKGYQPGQEFRP
PPDWDAGFEFSGRGFGGQETADFSDFFAELFGGLGGGRGSPFHTRGGRAGYQARGEDHHAKVVLDLADAFTGATRQVSLR
APEVDPQGHVVLKTRTLNVKIPKGVREGQVIRLAGQGAPGAGGAAAGDLLLEIHFKQDSRLRAVGRDLYLTLPVAPWEAA
LGAVVAVELPGGAVKVRIPEGAQSGKQLRVRGKGIPGEPPGDLLLDLRVVLPPADTPQAKAFYESMAREFAFDPRGRN

Sequences:

>Translated_318_residues
MEFKDYYKILGVPRDATADDIKKAFRKLARKYHPDISKEAGAETRMQEINEANAVLSDPEKRAAYDQLGKGYQPGQEFRP
PPDWDAGFEFSGRGFGGQETADFSDFFAELFGGLGGGRGSPFHTRGGRAGYQARGEDHHAKVVLDLADAFTGATRQVSLR
APEVDPQGHVVLKTRTLNVKIPKGVREGQVIRLAGQGAPGAGGAAAGDLLLEIHFKQDSRLRAVGRDLYLTLPVAPWEAA
LGAVVAVELPGGAVKVRIPEGAQSGKQLRVRGKGIPGEPPGDLLLDLRVVLPPADTPQAKAFYESMAREFAFDPRGRN
>Mature_318_residues
MEFKDYYKILGVPRDATADDIKKAFRKLARKYHPDISKEAGAETRMQEINEANAVLSDPEKRAAYDQLGKGYQPGQEFRP
PPDWDAGFEFSGRGFGGQETADFSDFFAELFGGLGGGRGSPFHTRGGRAGYQARGEDHHAKVVLDLADAFTGATRQVSLR
APEVDPQGHVVLKTRTLNVKIPKGVREGQVIRLAGQGAPGAGGAAAGDLLLEIHFKQDSRLRAVGRDLYLTLPVAPWEAA
LGAVVAVELPGGAVKVRIPEGAQSGKQLRVRGKGIPGEPPGDLLLDLRVVLPPADTPQAKAFYESMAREFAFDPRGRN

Specific function: DNA-binding protein that preferentially recognizes a curved DNA sequence. It is probably a functional analog of DnaJ; displays overlapping activities with DnaJ, but functions under different conditions, probably acting as a molecular chaperone in an adapt

COG id: COG2214

COG function: function code O; DnaJ-class molecular chaperone

Gene ontology:

Cell location: Cytoplasm, nucleoid [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain [H]

Homologues:

Organism=Homo sapiens, GI201862518, Length=339, Percent_Identity=29.2035398230088, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI56549115, Length=339, Percent_Identity=29.2035398230088, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI6631085, Length=332, Percent_Identity=28.9156626506024, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI201862587, Length=339, Percent_Identity=29.2035398230088, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI5453690, Length=325, Percent_Identity=28.6153846153846, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI39204547, Length=316, Percent_Identity=29.1139240506329, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI205360838, Length=348, Percent_Identity=24.7126436781609, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI205360840, Length=348, Percent_Identity=24.7126436781609, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI7706495, Length=324, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI72534740, Length=137, Percent_Identity=40.1459854014599, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI194306642, Length=115, Percent_Identity=43.4782608695652, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI194306640, Length=115, Percent_Identity=43.4782608695652, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4504511, Length=323, Percent_Identity=25.3869969040248, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI9558755, Length=104, Percent_Identity=44.2307692307692, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI56687498, Length=65, Percent_Identity=52.3076923076923, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI194328760, Length=155, Percent_Identity=33.5483870967742, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI194328758, Length=155, Percent_Identity=33.5483870967742, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI47777312, Length=116, Percent_Identity=40.5172413793103, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI22749377, Length=66, Percent_Identity=51.5151515151515, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI88501736, Length=67, Percent_Identity=50.7462686567164, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI27151736, Length=67, Percent_Identity=50.7462686567164, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI4885495, Length=111, Percent_Identity=44.1441441441441, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI17388799, Length=111, Percent_Identity=44.1441441441441, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI45504382, Length=64, Percent_Identity=50, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI5031741, Length=64, Percent_Identity=51.5625, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI23503241, Length=115, Percent_Identity=37.3913043478261, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787235, Length=319, Percent_Identity=40.4388714733542, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1786197, Length=372, Percent_Identity=33.0645161290323, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17534355, Length=341, Percent_Identity=28.4457478005865, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI212645553, Length=332, Percent_Identity=26.5060240963855, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17553096, Length=63, Percent_Identity=55.5555555555556, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17553098, Length=63, Percent_Identity=55.5555555555556, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17568643, Length=66, Percent_Identity=54.5454545454545, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115532476, Length=66, Percent_Identity=48.4848484848485, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323870, Length=69, Percent_Identity=53.6231884057971, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324265, Length=65, Percent_Identity=52.3076923076923, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323119, Length=68, Percent_Identity=51.4705882352941, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323813, Length=110, Percent_Identity=41.8181818181818, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322388, Length=99, Percent_Identity=42.4242424242424, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI85726398, Length=351, Percent_Identity=29.6296296296296, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24658562, Length=327, Percent_Identity=30.2752293577982, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24658555, Length=327, Percent_Identity=30.2752293577982, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19920464, Length=315, Percent_Identity=27.3015873015873, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24646562, Length=277, Percent_Identity=28.8808664259928, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24646560, Length=277, Percent_Identity=28.8808664259928, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24646558, Length=277, Percent_Identity=28.8808664259928, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24646556, Length=277, Percent_Identity=28.8808664259928, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21357547, Length=277, Percent_Identity=28.8808664259928, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI18858081, Length=313, Percent_Identity=27.1565495207668, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24668492, Length=67, Percent_Identity=49.2537313432836, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45549272, Length=68, Percent_Identity=52.9411764705882, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24584630, Length=109, Percent_Identity=39.4495412844037, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45552813, Length=68, Percent_Identity=52.9411764705882, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45552811, Length=68, Percent_Identity=52.9411764705882, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17648033, Length=109, Percent_Identity=39.4495412844037, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17737735, Length=64, Percent_Identity=51.5625, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574723, Length=64, Percent_Identity=51.5625, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24668523, Length=64, Percent_Identity=51.5625, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24654066, Length=68, Percent_Identity=52.9411764705882, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24654070, Length=68, Percent_Identity=52.9411764705882, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24654068, Length=68, Percent_Identity=52.9411764705882, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24654072, Length=68, Percent_Identity=52.9411764705882, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20129487, Length=157, Percent_Identity=35.6687898089172, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24584014, Length=157, Percent_Identity=35.6687898089172, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21357687, Length=64, Percent_Identity=46.875, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161077138, Length=64, Percent_Identity=53.125, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002939
- InterPro:   IPR001623
- InterPro:   IPR018253
- InterPro:   IPR008971
- InterPro:   IPR015609
- InterPro:   IPR003095 [H]

Pfam domain/function: PF00226 DnaJ; PF01556 DnaJ_C [H]

EC number: NA

Molecular weight: Translated: 34295; Mature: 34295

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50076 DNAJ_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKDYYKILGVPRDATADDIKKAFRKLARKYHPDISKEAGAETRMQEINEANAVLSDPE
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHCCCH
KRAAYDQLGKGYQPGQEFRPPPDWDAGFEFSGRGFGGQETADFSDFFAELFGGLGGGRGS
HHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
PFHTRGGRAGYQARGEDHHAKVVLDLADAFTGATRQVSLRAPEVDPQGHVVLKTRTLNVK
CCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEEEE
IPKGVREGQVIRLAGQGAPGAGGAAAGDLLLEIHFKQDSRLRAVGRDLYLTLPVAPWEAA
CCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEECCCCHHHHCCCEEEEEECCCCHHHH
LGAVVAVELPGGAVKVRIPEGAQSGKQLRVRGKGIPGEPPGDLLLDLRVVLPPADTPQAK
CCEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCHHHH
AFYESMAREFAFDPRGRN
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEFKDYYKILGVPRDATADDIKKAFRKLARKYHPDISKEAGAETRMQEINEANAVLSDPE
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHCCCH
KRAAYDQLGKGYQPGQEFRPPPDWDAGFEFSGRGFGGQETADFSDFFAELFGGLGGGRGS
HHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
PFHTRGGRAGYQARGEDHHAKVVLDLADAFTGATRQVSLRAPEVDPQGHVVLKTRTLNVK
CCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEEEE
IPKGVREGQVIRLAGQGAPGAGGAAAGDLLLEIHFKQDSRLRAVGRDLYLTLPVAPWEAA
CCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEECCCCHHHHCCCEEEEEECCCCHHHH
LGAVVAVELPGGAVKVRIPEGAQSGKQLRVRGKGIPGEPPGDLLLDLRVVLPPADTPQAK
CCEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCHHHH
AFYESMAREFAFDPRGRN
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA