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The map label for this gene is oprM [H]

Identifier: 74317434

GI number: 74317434

Start: 1503810

End: 1505219

Strand: Reverse

Name: oprM [H]

Synonym: Tbd_1416

Alternate gene names: 74317434

Gene position: 1505219-1503810 (Counterclockwise)

Preceding gene: 74317435

Following gene: 74317433

Centisome position: 51.73

GC content: 71.13

Gene sequence:

>1410_bases
ATGTCCCGCCTTCCCAGCCTGAGCGTGACGCTCGCCGCAGCGTTCTTGAGCGCGTGCAGCATGATGCCGCGCTACGAGCG
GCCCGCCGCGCCCGTCCCGGACGCCTTCCCCGGTTCTGCGCTGGCGAGCCCGGCGACCGCCGACGCACCGCCTGCGGACG
CGATCGCTTGGCGCGACTACTTCGCCGACCCGCACCTGCGCGAGGTGATCGCACTCGCGCTCGCCAACAACCGCGACCTG
CGCGTCGCGGCGCTCAACATCGAGCGCGCCCGTGCGCAGTACCGGATCCAGCGCGCCGATCTGTTTCCCGCAGTCAGTGC
CGACGGCAGCGAAGTCGCGCAACGCTTGCCGCGCACGCTCAGCACGACCGGCGACGCCACGGTGGTGCGCCAGTACAGCG
CGACGATTGGTTTTTCGGCTTACGAGCTCGATTTCTTCGGGCGCATCCGCAGCCTCAACGCCGAGGCGCTCGAACGCTAC
CTCGGCACCGAGGAAGCGCGCCGCAGCGCCCAGATCAGCCTCGTCGCCGAAGTCGCCAACACCTGGCTGTTGCTCGGTTT
CGACCGCGAACGCCTCGCGCTCGCGCGCAACACCTACGAAACCCGGCAGAAGTCGCACGATCTCACGCGCCGCAGCTTCG
AGGCCGGCGCGGTCTCGGCGCTCGACCTCAACCAGTCGCAGACGCTGCTGCAGACGGCGCGTGCGGACGTGGCGCAGTTC
AGCGCCTTCGTCGCGCAGGACGCGAACCTGCTCGCGCTGCTCGTCGGCGCGCCGGTTCCCGACACGCTGCAACCGGCGCG
GCTGCCGGACGGTGTTAGCGCGGTCGCCGAGCTGCCGGCCGGCGTGCCGTCGGAGGTGCTGGCCCGCCGGCCGGACATCC
TGCAGGCCGAACGTGCGCTGCGGGCGGCGAACGCGAGCATCGGCGCCGCGCGGGCGGCGTTCTTCCCGAGCATCAGTCTG
ACCGCGGAGGCCGGCACGGCAAGCAGCACGCTCGACGGCCTGTTCGAAAGCGGGTCGGGCACCTGGCGCTTCATCCCGCA
GATCCGCATCCCGATTTTCGAGGCCGGGCGCCTGCGCGCGAGTCTCGACGTCGCCGAGGTCCAGCGCGACATCAACGTCG
CGCAGTATGAACGCGCGATTCAGGCCGCCTTTCGCGAAGTCGCCGACGCGCTCGCCGAGCGCGCGACGCTCAGCGAGCGT
CTCGACGCGCGGCAAAAGCAGGTCGAGGCGACGCGCGCGGGCTTCGGCCTGTCCGAGGCGCGCTACAAGAGCGGCGTCGA
CAGCTACCTCGGTCTGCTCGACGCGCAGCGCAGCCTCTATGCGGCCGAGCAGGCGCTGATCGCCGAGCGGCTCGTCGACG
CGACCAATCGCGTGACGCTCTATCGCACGCTCGGCGGAGGCTGGCGCTGA

Upstream 100 bases:

>100_bases
TCTTCATCCCGCTGTTCTACGTGGTTATCAAGCGGGTATTCAAGGACAAGCCGCGGCCAGGCAGCGTCGCCGCCGCCCCC
GTGCCGCAGGAGCTCAAGTG

Downstream 100 bases:

>100_bases
CAGTGCGCGGTCGTGAAGGGCAGCCGCGCAGACGCTATACAATTTCGAGGCTTACTTTCGAACCATCGGGCAGCTCCTTG
AATAAGCAGCCATTTCGCAC

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSRLPSLSVTLAAAFLSACSMMPRYERPAAPVPDAFPGSALASPATADAPPADAIAWRDYFADPHLREVIALALANNRDL
RVAALNIERARAQYRIQRADLFPAVSADGSEVAQRLPRTLSTTGDATVVRQYSATIGFSAYELDFFGRIRSLNAEALERY
LGTEEARRSAQISLVAEVANTWLLLGFDRERLALARNTYETRQKSHDLTRRSFEAGAVSALDLNQSQTLLQTARADVAQF
SAFVAQDANLLALLVGAPVPDTLQPARLPDGVSAVAELPAGVPSEVLARRPDILQAERALRAANASIGAARAAFFPSISL
TAEAGTASSTLDGLFESGSGTWRFIPQIRIPIFEAGRLRASLDVAEVQRDINVAQYERAIQAAFREVADALAERATLSER
LDARQKQVEATRAGFGLSEARYKSGVDSYLGLLDAQRSLYAAEQALIAERLVDATNRVTLYRTLGGGWR

Sequences:

>Translated_469_residues
MSRLPSLSVTLAAAFLSACSMMPRYERPAAPVPDAFPGSALASPATADAPPADAIAWRDYFADPHLREVIALALANNRDL
RVAALNIERARAQYRIQRADLFPAVSADGSEVAQRLPRTLSTTGDATVVRQYSATIGFSAYELDFFGRIRSLNAEALERY
LGTEEARRSAQISLVAEVANTWLLLGFDRERLALARNTYETRQKSHDLTRRSFEAGAVSALDLNQSQTLLQTARADVAQF
SAFVAQDANLLALLVGAPVPDTLQPARLPDGVSAVAELPAGVPSEVLARRPDILQAERALRAANASIGAARAAFFPSISL
TAEAGTASSTLDGLFESGSGTWRFIPQIRIPIFEAGRLRASLDVAEVQRDINVAQYERAIQAAFREVADALAERATLSER
LDARQKQVEATRAGFGLSEARYKSGVDSYLGLLDAQRSLYAAEQALIAERLVDATNRVTLYRTLGGGWR
>Mature_468_residues
SRLPSLSVTLAAAFLSACSMMPRYERPAAPVPDAFPGSALASPATADAPPADAIAWRDYFADPHLREVIALALANNRDLR
VAALNIERARAQYRIQRADLFPAVSADGSEVAQRLPRTLSTTGDATVVRQYSATIGFSAYELDFFGRIRSLNAEALERYL
GTEEARRSAQISLVAEVANTWLLLGFDRERLALARNTYETRQKSHDLTRRSFEAGAVSALDLNQSQTLLQTARADVAQFS
AFVAQDANLLALLVGAPVPDTLQPARLPDGVSAVAELPAGVPSEVLARRPDILQAERALRAANASIGAARAAFFPSISLT
AEAGTASSTLDGLFESGSGTWRFIPQIRIPIFEAGRLRASLDVAEVQRDINVAQYERAIQAAFREVADALAERATLSERL
DARQKQVEATRAGFGLSEARYKSGVDSYLGLLDAQRSLYAAEQALIAERLVDATNRVTLYRTLGGGWR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=470, Percent_Identity=40, Blast_Score=334, Evalue=8e-93,
Organism=Escherichia coli, GI2367348, Length=493, Percent_Identity=26.369168356998, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50721; Mature: 50589

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLPSLSVTLAAAFLSACSMMPRYERPAAPVPDAFPGSALASPATADAPPADAIAWRDY
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
FADPHLREVIALALANNRDLRVAALNIERARAQYRIQRADLFPAVSADGSEVAQRLPRTL
CCCHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHH
STTGDATVVRQYSATIGFSAYELDFFGRIRSLNAEALERYLGTEEARRSAQISLVAEVAN
CCCCCHHHHHHHHHHHCCCEEEHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHC
TWLLLGFDRERLALARNTYETRQKSHDLTRRSFEAGAVSALDLNQSQTLLQTARADVAQF
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
SAFVAQDANLLALLVGAPVPDTLQPARLPDGVSAVAELPAGVPSEVLARRPDILQAERAL
HHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHH
RAANASIGAARAAFFPSISLTAEAGTASSTLDGLFESGSGTWRFIPQIRIPIFEAGRLRA
HHHCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCEEECCEEECCEECCCCHHH
SLDVAEVQRDINVAQYERAIQAAFREVADALAERATLSERLDARQKQVEATRAGFGLSEA
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RYKSGVDSYLGLLDAQRSLYAAEQALIAERLVDATNRVTLYRTLGGGWR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure 
SRLPSLSVTLAAAFLSACSMMPRYERPAAPVPDAFPGSALASPATADAPPADAIAWRDY
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
FADPHLREVIALALANNRDLRVAALNIERARAQYRIQRADLFPAVSADGSEVAQRLPRTL
CCCHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHH
STTGDATVVRQYSATIGFSAYELDFFGRIRSLNAEALERYLGTEEARRSAQISLVAEVAN
CCCCCHHHHHHHHHHHCCCEEEHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHC
TWLLLGFDRERLALARNTYETRQKSHDLTRRSFEAGAVSALDLNQSQTLLQTARADVAQF
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
SAFVAQDANLLALLVGAPVPDTLQPARLPDGVSAVAELPAGVPSEVLARRPDILQAERAL
HHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHH
RAANASIGAARAAFFPSISLTAEAGTASSTLDGLFESGSGTWRFIPQIRIPIFEAGRLRA
HHHCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCEEECCEEECCEECCCCHHH
SLDVAEVQRDINVAQYERAIQAAFREVADALAERATLSERLDARQKQVEATRAGFGLSEA
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RYKSGVDSYLGLLDAQRSLYAAEQALIAERLVDATNRVTLYRTLGGGWR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]