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The map label for this gene is narY [H]

Identifier: 74317422

GI number: 74317422

Start: 1488549

End: 1490117

Strand: Direct

Name: narY [H]

Synonym: Tbd_1404

Alternate gene names: 74317422

Gene position: 1488549-1490117 (Clockwise)

Preceding gene: 74317421

Following gene: 74317423

Centisome position: 51.16

GC content: 62.14

Gene sequence:

>1569_bases
ATGAAAGTCAGAGCGCAATTCGCCTTCGTCTTCAACCTGGACAAGTGCATCGGATGTCACACCTGCTCGGTGACCTGCAA
GAACGTCTGGACCAACCGCAAGGGCGTCGAGTACGCCTGGTTCAACAACGTCGAGTCCAAGCCCGGCGTCGGCTACCCCA
AAAGCTGGGAAGACCAGAGCAAGTGGAAAGGCGGCTGGGAGCTCAGGAACGGCAAGTTGGAGTTGAAATCCGGCAACCGC
CTATCCAAGCTCGTGAACATCTTCGCCAACCCGGACATGCCGGAGATCGAGGACTACTACGAACCCTTCACTTACAACTA
CGCGCATTTGCAGAACGCGCCGCTGTCGGAGGCCGCTCCGACCGCGCGCCCGATGTCGCAGATCGACGGCAGCCGGATGG
AGAAAATCCAGTGGGGCCCGAACTGGGAAGACGACCTCGCCGGCGAGTTCGCCAAGCGCAGCAAGGACAAGAACCTCGAC
AACATCCAGAAGGAGATGTACGCCCAGTTCGAGAACACCTTCCACATGTATCTGCCGCGCATCTGCAATCACTGCCTGAA
TCCGGCCTGCGTGGCGGCGTGTCCGTCGGGTTCGATCTACAAGCGCGAAGAGGACGGCATCGTGCTCGTCGACCAGGACA
AATGCCGCGGCTGGCGCATGTGCATTTCCTCCTGCCCGTACAAGAAGGTGTTCTACAACTGGGAATCCGGCAAGGCCGAG
AAATGCATCGGCTGCTATCCGCGCGTCGAGTCCGGGATGCCGACCGTATGCTCGGAATCCTGCGTCGGGCGCATCCGCTA
CAACGGCATCATGCTGTATGACGCCGACAAGATCGAAGCGCTCGCCAGCACCGAGAACGAGCAGGACCTGTACGAGGCGC
AGTGCAACATCTTCCTCGACCCGCACGATCCTGAAGTCATCGCCGCCGCGCGCGCCGAGGGCATCAACGAGGACTGGATC
GTCGCGGCACAGAAGTCGCCGATCTACAAGATGGCGATCGACTGGAAGATCGCCTTCCCGATGCACCCGGAGTTCCGCAC
GCTGCCGATGGTCTGGTACGTGCCGCCGCTCTCACCCGTACAGTCGCAAATCGACCAGGGCAATCTGCCGGTCGGCCCCG
ACGGCGCGATTCCGCTGGCCGGCACGATGCGCACGCCGATCCAGTACCTCGCCAACCTGCTGACCGCCGGCAAGACCGCA
CCGATCGAAAGCGCGCTCAACCGCCTGATCGCGATGCGCAGCTACCAACGCTCGGTGCACGTCGAGGGCGTGGCCGATAC
CCGCGCCCTCGACAAGGCCGGCATCACCGAGGACCAGGCCAAGGAGATGTATCGCTACCTCGCGATAGCCAACTACGAGG
ACCGTTTCGTGATCCCGACCGGGCACGAGGAAGTGCGCCTCGAAGACTTCCACGGCTTCCAGGGACAGAACGGCTTCACC
TTCGGCAACGACTCATCGGCCGGCATTTCGGTCAACTCGCTGTTCCCCGAGCGGCGCAAGGAGACCGTGGAGCCGAGGGA
AGAGGCGCCCTCGCGCCGCGTCATTCCCATCGTTCGCAGCGACTACTAA

Upstream 100 bases:

>100_bases
ATCGCGGACAAGGACGTGGACTGGCTCGAACGTCCGCTCACGCCCGAGCGTGAAGCGCAACGCAATCCCAACGGCATCGG
CACCAAGTAAGGAGAACCCC

Downstream 100 bases:

>100_bases
GGAGCGCACCATGACGGACGCCCCCATGACGCTCTTCAAGCTGCTCTCCGCGCTGCTCGAGTATCCGGACCAGGCCTTGA
TGCAGGCCATTCCCGAACTC

Product: respiratory nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MKVRAQFAFVFNLDKCIGCHTCSVTCKNVWTNRKGVEYAWFNNVESKPGVGYPKSWEDQSKWKGGWELRNGKLELKSGNR
LSKLVNIFANPDMPEIEDYYEPFTYNYAHLQNAPLSEAAPTARPMSQIDGSRMEKIQWGPNWEDDLAGEFAKRSKDKNLD
NIQKEMYAQFENTFHMYLPRICNHCLNPACVAACPSGSIYKREEDGIVLVDQDKCRGWRMCISSCPYKKVFYNWESGKAE
KCIGCYPRVESGMPTVCSESCVGRIRYNGIMLYDADKIEALASTENEQDLYEAQCNIFLDPHDPEVIAAARAEGINEDWI
VAAQKSPIYKMAIDWKIAFPMHPEFRTLPMVWYVPPLSPVQSQIDQGNLPVGPDGAIPLAGTMRTPIQYLANLLTAGKTA
PIESALNRLIAMRSYQRSVHVEGVADTRALDKAGITEDQAKEMYRYLAIANYEDRFVIPTGHEEVRLEDFHGFQGQNGFT
FGNDSSAGISVNSLFPERRKETVEPREEAPSRRVIPIVRSDY

Sequences:

>Translated_522_residues
MKVRAQFAFVFNLDKCIGCHTCSVTCKNVWTNRKGVEYAWFNNVESKPGVGYPKSWEDQSKWKGGWELRNGKLELKSGNR
LSKLVNIFANPDMPEIEDYYEPFTYNYAHLQNAPLSEAAPTARPMSQIDGSRMEKIQWGPNWEDDLAGEFAKRSKDKNLD
NIQKEMYAQFENTFHMYLPRICNHCLNPACVAACPSGSIYKREEDGIVLVDQDKCRGWRMCISSCPYKKVFYNWESGKAE
KCIGCYPRVESGMPTVCSESCVGRIRYNGIMLYDADKIEALASTENEQDLYEAQCNIFLDPHDPEVIAAARAEGINEDWI
VAAQKSPIYKMAIDWKIAFPMHPEFRTLPMVWYVPPLSPVQSQIDQGNLPVGPDGAIPLAGTMRTPIQYLANLLTAGKTA
PIESALNRLIAMRSYQRSVHVEGVADTRALDKAGITEDQAKEMYRYLAIANYEDRFVIPTGHEEVRLEDFHGFQGQNGFT
FGNDSSAGISVNSLFPERRKETVEPREEAPSRRVIPIVRSDY
>Mature_522_residues
MKVRAQFAFVFNLDKCIGCHTCSVTCKNVWTNRKGVEYAWFNNVESKPGVGYPKSWEDQSKWKGGWELRNGKLELKSGNR
LSKLVNIFANPDMPEIEDYYEPFTYNYAHLQNAPLSEAAPTARPMSQIDGSRMEKIQWGPNWEDDLAGEFAKRSKDKNLD
NIQKEMYAQFENTFHMYLPRICNHCLNPACVAACPSGSIYKREEDGIVLVDQDKCRGWRMCISSCPYKKVFYNWESGKAE
KCIGCYPRVESGMPTVCSESCVGRIRYNGIMLYDADKIEALASTENEQDLYEAQCNIFLDPHDPEVIAAARAEGINEDWI
VAAQKSPIYKMAIDWKIAFPMHPEFRTLPMVWYVPPLSPVQSQIDQGNLPVGPDGAIPLAGTMRTPIQYLANLLTAGKTA
PIESALNRLIAMRSYQRSVHVEGVADTRALDKAGITEDQAKEMYRYLAIANYEDRFVIPTGHEEVRLEDFHGFQGQNGFT
FGNDSSAGISVNSLFPERRKETVEPREEAPSRRVIPIVRSDY

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=508, Percent_Identity=63.5826771653543, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=64.0316205533597, Blast_Score=694, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=91, Percent_Identity=51.6483516483517, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787122, Length=91, Percent_Identity=50.5494505494505, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI2367345, Length=104, Percent_Identity=31.7307692307692, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=37.3737373737374, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI226510944, Length=92, Percent_Identity=34.7826086956522, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 59196; Mature: 59196

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRAQFAFVFNLDKCIGCHTCSVTCKNVWTNRKGVEYAWFNNVESKPGVGYPKSWEDQS
CCEEEEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHH
KWKGGWELRNGKLELKSGNRLSKLVNIFANPDMPEIEDYYEPFTYNYAHLQNAPLSEAAP
HCCCCEEEECCEEEECCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHCCCCCCCCCC
TARPMSQIDGSRMEKIQWGPNWEDDLAGEFAKRSKDKNLDNIQKEMYAQFENTFHMYLPR
CCCCHHHCCCCHHHEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ICNHCLNPACVAACPSGSIYKREEDGIVLVDQDKCRGWRMCISSCPYKKVFYNWESGKAE
HHHHHCCCCEEEECCCCCCEEECCCCEEEEECCCCCHHHHHHHCCCCCCEEEECCCCCCC
KCIGCYPRVESGMPTVCSESCVGRIRYNGIMLYDADKIEALASTENEQDLYEAQCNIFLD
CCEECCCCCCCCCCCHHHHHHHCEEEECCEEEEECHHHHHHHCCCCHHHHHHCCCEEEEC
PHDPEVIAAARAEGINEDWIVAAQKSPIYKMAIDWKIAFPMHPEFRTLPMVWYVPPLSPV
CCCCCEEEHHHHCCCCCCEEEEECCCCEEEEEEEEEEECCCCCCCCCCCEEEEECCCCHH
QSQIDQGNLPVGPDGAIPLAGTMRTPIQYLANLLTAGKTAPIESALNRLIAMRSYQRSVH
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEE
VEGVADTRALDKAGITEDQAKEMYRYLAIANYEDRFVIPTGHEEVRLEDFHGFQGQNGFT
ECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCCCC
FGNDSSAGISVNSLFPERRKETVEPREEAPSRRVIPIVRSDY
CCCCCCCCEEHHHCCCHHHHHCCCHHHHCCCCCEEEEECCCC
>Mature Secondary Structure
MKVRAQFAFVFNLDKCIGCHTCSVTCKNVWTNRKGVEYAWFNNVESKPGVGYPKSWEDQS
CCEEEEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHH
KWKGGWELRNGKLELKSGNRLSKLVNIFANPDMPEIEDYYEPFTYNYAHLQNAPLSEAAP
HCCCCEEEECCEEEECCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHCCCCCCCCCC
TARPMSQIDGSRMEKIQWGPNWEDDLAGEFAKRSKDKNLDNIQKEMYAQFENTFHMYLPR
CCCCHHHCCCCHHHEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ICNHCLNPACVAACPSGSIYKREEDGIVLVDQDKCRGWRMCISSCPYKKVFYNWESGKAE
HHHHHCCCCEEEECCCCCCEEECCCCEEEEECCCCCHHHHHHHCCCCCCEEEECCCCCCC
KCIGCYPRVESGMPTVCSESCVGRIRYNGIMLYDADKIEALASTENEQDLYEAQCNIFLD
CCEECCCCCCCCCCCHHHHHHHCEEEECCEEEEECHHHHHHHCCCCHHHHHHCCCEEEEC
PHDPEVIAAARAEGINEDWIVAAQKSPIYKMAIDWKIAFPMHPEFRTLPMVWYVPPLSPV
CCCCCEEEHHHHCCCCCCEEEEECCCCEEEEEEEEEEECCCCCCCCCCCEEEEECCCCHH
QSQIDQGNLPVGPDGAIPLAGTMRTPIQYLANLLTAGKTAPIESALNRLIAMRSYQRSVH
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEE
VEGVADTRALDKAGITEDQAKEMYRYLAIANYEDRFVIPTGHEEVRLEDFHGFQGQNGFT
ECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCCCC
FGNDSSAGISVNSLFPERRKETVEPREEAPSRRVIPIVRSDY
CCCCCCCCEEHHHCCCHHHHHCCCHHHHCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]