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The map label for this gene is hupB [H]

Identifier: 74317393

GI number: 74317393

Start: 1454962

End: 1456752

Strand: Reverse

Name: hupB [H]

Synonym: Tbd_1375

Alternate gene names: 74317393

Gene position: 1456752-1454962 (Counterclockwise)

Preceding gene: 74317394

Following gene: 74317392

Centisome position: 50.06

GC content: 65.77

Gene sequence:

>1791_bases
ATGGCAACAACACAGCGCGTCGTCGTCGATCCCATCACGCGCATCGAGGGCCATCTGCGCATCGAGGCCGAGACCGACGT
CGACGGCAGGATCCTCAAGGCGTACTCGGCCGGAACCATGGTGCGCGGGATCGAGATCATCCTGCGGGGCCGCGATCCGC
GCGACGCGTGGGCCTTCGCGCAGCGCATCTGCGGGGTCTGCACGCTCGTGCACGGGGTCGCCTCGGTGCGCTCGGTCGAG
GATGCGCTGCACCGCGCGATCCCCGGCTATGCGCTGCCGCCGAACGCGGAGCTGATCCGCAACCTGATGATCGCGGCGCA
GCACGTCCACGACCACGTCATGCACTTCTATCACCTGCACGCACTCGACTGGGTCGACGTCGTCTCGGCCTTGAAGGCCG
ATCCGCGCGCGGCCTCGACGCTGGCGCAGTCGATCTCGGACTACTCGCGGTCGTCGCCCGGGTATTTCGCCGACGTGCAG
CGAAAGGTGAAGACCTTCGTCGAACAGGGTCAGCTCGGAATCTTCGCCAACGGCTACTGGGGCCACCCGGCCTACAAGCT
GCCGCCCGAAGCGAACCTGATGGCGCTCGCGCATTATCTCGACGCGCTCGCCTGGCAGCGTGAGGTCGTCAAGCTGCACG
CGATCTTCGGCGGCCGCAATCCGCATCCGAATTTCGTCGTCGGCGGCGTGCCGTCGGCGATCTCGGTCTTTCCCACCGAG
GGCCGCGGCAAGGGGCCGCATTACCGCGGCGGCCAGGACGCGACCGCGGTCAACATGGTCGGCCTGCAGACGGTGCAGAC
CGTCATTCGCCAGATGCGCGAGTTCGTCGACCAGGTCTATCTGCCGGACACCCTTGCGATCGCCCGCTACTACAAGGACT
GGGCGGGGCGCGGCGAGGGCCTGGGCAATTTCCTGTCCTTCGGCGACCTGCCGTCCAAGGGATTCTGGGATACGGATTCC
TATTTCATTCCGCGCGGCGTCATTCTCGATCGTGACCTGACGCGGGTCCACCCGATCGACCTCGACGACGACAAGGAAAT
CCAGGAATTCGTCAGTCACTCCTGGTATCGCTACGAAGGCGGCGACGCCCAGGGCCTGCACCCGTTCAAGGGCGAAACCG
CGCTGAACTACACCGGACCCAAGCCGCCCTATGAGCAGCTCGACGTCGCGCAGAAATACTCGTGGATGAAGTCGCCGCGC
TGGAAGGGCAAATCGATGGAAGTCGGCCCGCTCGCACGCGTGCTCGTGATGGTCGCCAAGGGCGACAAACAGGCGAAGGA
ACTCGTCGACCGCGCGCTGAAGACGCTCGATCTGCCGGTCACGGCGCTCTATTCGACGCTGGGGCGGACCGCGGCACGCA
CGCTCGAGTCGAAAATACTCGCCGACGCGATGCAGGGCTGGTTCGATCAGCTCATGGCGAACATCAAGGCCGGCGACCTG
CGCACCTTCAACGAAAAATACTGGGATCCCTCGACCTGGCCAGCGCACATGCAAGGCGTCGGCCTCATGGAGGCGCCACG
CGGCGGCTTGGCCCACTGGATCGTCGTCGACGACGGCAGGATCAGCAACTACCAGGCGGTCGTCCCGAGCACCTGGAATG
CGGGCCCGCGCGACGCGGCCGGGCAGCCGGGCGCCTACGAGGCGGCCCTGCAGCACAACCACACGATGCTCGACCCGAAG
CAGCCGATCGAGATCCTGCGCACGATCCATTCCTTCGACCCCTGCATCGCCTGCGCCGTCCACGTGGTCGATCCGGACGG
TGAGGAACTCGTGCAGGTGAAGGTCAAATAA

Upstream 100 bases:

>100_bases
TGCCGCATCACGATGGAAGAGGCAGTCGACCATTACGGCATGAATACCAGGCTGCTCGGCTTGACCGAGCTCATGGCGGA
GCATCTGGAGGACTGAGTTC

Downstream 100 bases:

>100_bases
CCGAATCCCCTGGAGCCACCCATGTACCGAAATACGATCAGCCGCTGTATCGCGCCCGTCCTCGCGCTGCTCTCCGGTAG
CGCCGCCGCACACAGCCTCG

Product: Ni-Fe hydrogenase large chain

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MATTQRVVVDPITRIEGHLRIEAETDVDGRILKAYSAGTMVRGIEIILRGRDPRDAWAFAQRICGVCTLVHGVASVRSVE
DALHRAIPGYALPPNAELIRNLMIAAQHVHDHVMHFYHLHALDWVDVVSALKADPRAASTLAQSISDYSRSSPGYFADVQ
RKVKTFVEQGQLGIFANGYWGHPAYKLPPEANLMALAHYLDALAWQREVVKLHAIFGGRNPHPNFVVGGVPSAISVFPTE
GRGKGPHYRGGQDATAVNMVGLQTVQTVIRQMREFVDQVYLPDTLAIARYYKDWAGRGEGLGNFLSFGDLPSKGFWDTDS
YFIPRGVILDRDLTRVHPIDLDDDKEIQEFVSHSWYRYEGGDAQGLHPFKGETALNYTGPKPPYEQLDVAQKYSWMKSPR
WKGKSMEVGPLARVLVMVAKGDKQAKELVDRALKTLDLPVTALYSTLGRTAARTLESKILADAMQGWFDQLMANIKAGDL
RTFNEKYWDPSTWPAHMQGVGLMEAPRGGLAHWIVVDDGRISNYQAVVPSTWNAGPRDAAGQPGAYEAALQHNHTMLDPK
QPIEILRTIHSFDPCIACAVHVVDPDGEELVQVKVK

Sequences:

>Translated_596_residues
MATTQRVVVDPITRIEGHLRIEAETDVDGRILKAYSAGTMVRGIEIILRGRDPRDAWAFAQRICGVCTLVHGVASVRSVE
DALHRAIPGYALPPNAELIRNLMIAAQHVHDHVMHFYHLHALDWVDVVSALKADPRAASTLAQSISDYSRSSPGYFADVQ
RKVKTFVEQGQLGIFANGYWGHPAYKLPPEANLMALAHYLDALAWQREVVKLHAIFGGRNPHPNFVVGGVPSAISVFPTE
GRGKGPHYRGGQDATAVNMVGLQTVQTVIRQMREFVDQVYLPDTLAIARYYKDWAGRGEGLGNFLSFGDLPSKGFWDTDS
YFIPRGVILDRDLTRVHPIDLDDDKEIQEFVSHSWYRYEGGDAQGLHPFKGETALNYTGPKPPYEQLDVAQKYSWMKSPR
WKGKSMEVGPLARVLVMVAKGDKQAKELVDRALKTLDLPVTALYSTLGRTAARTLESKILADAMQGWFDQLMANIKAGDL
RTFNEKYWDPSTWPAHMQGVGLMEAPRGGLAHWIVVDDGRISNYQAVVPSTWNAGPRDAAGQPGAYEAALQHNHTMLDPK
QPIEILRTIHSFDPCIACAVHVVDPDGEELVQVKVK
>Mature_595_residues
ATTQRVVVDPITRIEGHLRIEAETDVDGRILKAYSAGTMVRGIEIILRGRDPRDAWAFAQRICGVCTLVHGVASVRSVED
ALHRAIPGYALPPNAELIRNLMIAAQHVHDHVMHFYHLHALDWVDVVSALKADPRAASTLAQSISDYSRSSPGYFADVQR
KVKTFVEQGQLGIFANGYWGHPAYKLPPEANLMALAHYLDALAWQREVVKLHAIFGGRNPHPNFVVGGVPSAISVFPTEG
RGKGPHYRGGQDATAVNMVGLQTVQTVIRQMREFVDQVYLPDTLAIARYYKDWAGRGEGLGNFLSFGDLPSKGFWDTDSY
FIPRGVILDRDLTRVHPIDLDDDKEIQEFVSHSWYRYEGGDAQGLHPFKGETALNYTGPKPPYEQLDVAQKYSWMKSPRW
KGKSMEVGPLARVLVMVAKGDKQAKELVDRALKTLDLPVTALYSTLGRTAARTLESKILADAMQGWFDQLMANIKAGDLR
TFNEKYWDPSTWPAHMQGVGLMEAPRGGLAHWIVVDDGRISNYQAVVPSTWNAGPRDAAGQPGAYEAALQHNHTMLDPKQ
PIEILRTIHSFDPCIACAVHVVDPDGEELVQVKVK

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=600, Percent_Identity=52, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=603, Percent_Identity=46.1028192371476, Blast_Score=511, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 66191; Mature: 66060

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTQRVVVDPITRIEGHLRIEAETDVDGRILKAYSAGTMVRGIEIILRGRDPRDAWAFA
CCCCCCHHHCHHHHCCCEEEEEECCCCCCCEEEHHCCCCHHEEEEEEEECCCCHHHHHHH
QRICGVCTLVHGVASVRSVEDALHRAIPGYALPPNAELIRNLMIAAQHVHDHVMHFYHLH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALDWVDVVSALKADPRAASTLAQSISDYSRSSPGYFADVQRKVKTFVEQGQLGIFANGYW
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
GHPAYKLPPEANLMALAHYLDALAWQREVVKLHAIFGGRNPHPNFVVGGVPSAISVFPTE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCC
GRGKGPHYRGGQDATAVNMVGLQTVQTVIRQMREFVDQVYLPDTLAIARYYKDWAGRGEG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
LGNFLSFGDLPSKGFWDTDSYFIPRGVILDRDLTRVHPIDLDDDKEIQEFVSHSWYRYEG
CHHHHHCCCCCCCCCCCCCCEECCCCEEECCCCCEECCCCCCCCHHHHHHHHCCEEEECC
GDAQGLHPFKGETALNYTGPKPPYEQLDVAQKYSWMKSPRWKGKSMEVGPLARVLVMVAK
CCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
GDKQAKELVDRALKTLDLPVTALYSTLGRTAARTLESKILADAMQGWFDQLMANIKAGDL
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RTFNEKYWDPSTWPAHMQGVGLMEAPRGGLAHWIVVDDGRISNYQAVVPSTWNAGPRDAA
CHHHHHCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCC
GQPGAYEAALQHNHTMLDPKQPIEILRTIHSFDPCIACAVHVVDPDGEELVQVKVK
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCEEEEEEC
>Mature Secondary Structure 
ATTQRVVVDPITRIEGHLRIEAETDVDGRILKAYSAGTMVRGIEIILRGRDPRDAWAFA
CCCCCHHHCHHHHCCCEEEEEECCCCCCCEEEHHCCCCHHEEEEEEEECCCCHHHHHHH
QRICGVCTLVHGVASVRSVEDALHRAIPGYALPPNAELIRNLMIAAQHVHDHVMHFYHLH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALDWVDVVSALKADPRAASTLAQSISDYSRSSPGYFADVQRKVKTFVEQGQLGIFANGYW
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
GHPAYKLPPEANLMALAHYLDALAWQREVVKLHAIFGGRNPHPNFVVGGVPSAISVFPTE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCC
GRGKGPHYRGGQDATAVNMVGLQTVQTVIRQMREFVDQVYLPDTLAIARYYKDWAGRGEG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
LGNFLSFGDLPSKGFWDTDSYFIPRGVILDRDLTRVHPIDLDDDKEIQEFVSHSWYRYEG
CHHHHHCCCCCCCCCCCCCCEECCCCEEECCCCCEECCCCCCCCHHHHHHHHCCEEEECC
GDAQGLHPFKGETALNYTGPKPPYEQLDVAQKYSWMKSPRWKGKSMEVGPLARVLVMVAK
CCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
GDKQAKELVDRALKTLDLPVTALYSTLGRTAARTLESKILADAMQGWFDQLMANIKAGDL
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RTFNEKYWDPSTWPAHMQGVGLMEAPRGGLAHWIVVDDGRISNYQAVVPSTWNAGPRDAA
CHHHHHCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCC
GQPGAYEAALQHNHTMLDPKQPIEILRTIHSFDPCIACAVHVVDPDGEELVQVKVK
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275; 8163174 [H]