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The map label for this gene is merA [H]

Identifier: 74317359

GI number: 74317359

Start: 1422432

End: 1424057

Strand: Direct

Name: merA [H]

Synonym: Tbd_1341

Alternate gene names: 74317359

Gene position: 1422432-1424057 (Clockwise)

Preceding gene: 74317358

Following gene: 74317381

Centisome position: 48.88

GC content: 64.76

Gene sequence:

>1626_bases
ATGAGCGAACAGATTCTCCGCGTGACGGGCATGACCTGCGACCACTGTGCAGCGTCCGTGAAACAGGCACTGCTGAGGGT
GGCCGGTGTGACCCGTGCGGATGTGTCCTGTCCCGATGCCATGGCCCGCGTGGAGGTGGGTGTGGACACCCCCGTCGCCG
CACTGCTCAACGCCGTGCGTGCCAGGGGCTACGGCGCGGAGGTGGGTGGAGACGCCACGTCCAAGGCCTCCACCAAGCTC
AACGTCACCATTATCGGCGCCGGGTCGGCGGGCTTTGCCGCCGCCATCCGCGCGGCTGAAGAAGGCGCCGAAGTGACGGT
GATCGAAGCCTCCACCATCGGCGGCACCTGCGTAAACGTCGGCTGCGTGCCTTCGAAGATTCTGATCCGTGCCGCCCATG
TGGCGCACCTGCAAGCCCGACATCCTTTTCCCGGGCTGGTCAGGCATGCGCCGTTGCTGGATCGCACGGCGTTGGTCGCG
CAGCAGCAGGCGCGGGTGGAAGAACTGCGCCATGCGAAGTACGAACGCATTCTGGAAACCAACCCCGGCATCAAGCTGTT
GCGCGGCTTTGCGCGTTTCGAGGATGCCAAGACCCTCGTCGTCCGGCAACCGGATGATACGGAAAAGCGCCTCACGCCCG
ATCGAATCCTGATCGCCCCCGGCCGCTCGCCGCGGATTCCGGACGTGCCCGGCCTTGCCGGCACGCCGTTCTGGACTTCG
TCCGAGGCATTGATCGCCACGGAGACGCCGGCGCACCTGATCGTACACGGCGGTTCGGTGGTCGCGCTGGAACTCGCGCA
GGCTTTCCTGCGTCTGGGCAGCCGCGTTACGCTCGTTGCGCGCGGCAAGCTGTTGTCCAGGGAAGATCCGGCGATTGGCG
AAGGGCTCAATGCCGTGCTGGAGGAAGAGGGCATGCGGGTTCTCACGGATACCGACGTGAAAGCCGTACGCTTCGACGGT
ACGACATTTGAACTTGAAACAAGCGCGGGAACTCTCAGTGGCGACCGGCTGCTCGTTGCCACCGGCCGTAAGCCCAATAC
GGCGGGGCTCGCCCTCGACCGCACCGGCGTCACTACGGATACCAGCGGCGCCATCGTCGTGGACGATCATCTCCGCACTT
CGGTGCCGCACATCTATGCGGCCGGCGACTGCACCAATGCGCCGCAGTTCGTGTACGTGGCGGCGGCGGCTGGCACCCGC
GCGGCAATCAATATGACCGGCGGCGATGCGGCGCTGGATCTCTCCGTGGTGCCTGCAGTGGTGTTCACCGATCCGCAGGT
AGCCACGGTTGGCCTCACCGAAGCGCAGGCAAACAAGCTTGGCATGGAAACCGACAGCCGCACGCTCACACTCGACAACG
TGCCGCGCGCGCTGGCCAACTTCGACACGCGCGGTTTCATCAAGCTGGTGTCGGAGAAAAAAACCGGACGCCTGCTGGGC
GCGCAGGTGCTGGCAGGAGAAGGCGGCGAGATCATCCAGACCGCCGCGCTGGCTATCCAAAACCGCATGACGGTGGCCGA
TCTGGCGGGACAATTATTCCCCTACATCACCATGGGGGAAGGGTTGAAGCTTTGCGCCCAGACCTTTACCACGGACGTCA
AGCAGCTGTCCTGTTGTGCGGGATAA

Upstream 100 bases:

>100_bases
TCGACGATGCCAGCACCAGCATCAAAGCGCTGGAGGATGCCACCTTCGAGGCGGGCTATGAATCCACGGTCAAAGCCCCC
GCCAAAGGAGGGCAGGCCAA

Downstream 100 bases:

>100_bases
ATCACTCTGGCGCTTACGGTGCGAGCGGGTGTAGTCCTGGATTTGCGACCGGACTTCTTCGATCGTCTGAATCCGGCCGA
ATTGCAGCGATCTCTTCACT

Product: putative mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSEQILRVTGMTCDHCAASVKQALLRVAGVTRADVSCPDAMARVEVGVDTPVAALLNAVRARGYGAEVGGDATSKASTKL
NVTIIGAGSAGFAAAIRAAEEGAEVTVIEASTIGGTCVNVGCVPSKILIRAAHVAHLQARHPFPGLVRHAPLLDRTALVA
QQQARVEELRHAKYERILETNPGIKLLRGFARFEDAKTLVVRQPDDTEKRLTPDRILIAPGRSPRIPDVPGLAGTPFWTS
SEALIATETPAHLIVHGGSVVALELAQAFLRLGSRVTLVARGKLLSREDPAIGEGLNAVLEEEGMRVLTDTDVKAVRFDG
TTFELETSAGTLSGDRLLVATGRKPNTAGLALDRTGVTTDTSGAIVVDDHLRTSVPHIYAAGDCTNAPQFVYVAAAAGTR
AAINMTGGDAALDLSVVPAVVFTDPQVATVGLTEAQANKLGMETDSRTLTLDNVPRALANFDTRGFIKLVSEKKTGRLLG
AQVLAGEGGEIIQTAALAIQNRMTVADLAGQLFPYITMGEGLKLCAQTFTTDVKQLSCCAG

Sequences:

>Translated_541_residues
MSEQILRVTGMTCDHCAASVKQALLRVAGVTRADVSCPDAMARVEVGVDTPVAALLNAVRARGYGAEVGGDATSKASTKL
NVTIIGAGSAGFAAAIRAAEEGAEVTVIEASTIGGTCVNVGCVPSKILIRAAHVAHLQARHPFPGLVRHAPLLDRTALVA
QQQARVEELRHAKYERILETNPGIKLLRGFARFEDAKTLVVRQPDDTEKRLTPDRILIAPGRSPRIPDVPGLAGTPFWTS
SEALIATETPAHLIVHGGSVVALELAQAFLRLGSRVTLVARGKLLSREDPAIGEGLNAVLEEEGMRVLTDTDVKAVRFDG
TTFELETSAGTLSGDRLLVATGRKPNTAGLALDRTGVTTDTSGAIVVDDHLRTSVPHIYAAGDCTNAPQFVYVAAAAGTR
AAINMTGGDAALDLSVVPAVVFTDPQVATVGLTEAQANKLGMETDSRTLTLDNVPRALANFDTRGFIKLVSEKKTGRLLG
AQVLAGEGGEIIQTAALAIQNRMTVADLAGQLFPYITMGEGLKLCAQTFTTDVKQLSCCAG
>Mature_540_residues
SEQILRVTGMTCDHCAASVKQALLRVAGVTRADVSCPDAMARVEVGVDTPVAALLNAVRARGYGAEVGGDATSKASTKLN
VTIIGAGSAGFAAAIRAAEEGAEVTVIEASTIGGTCVNVGCVPSKILIRAAHVAHLQARHPFPGLVRHAPLLDRTALVAQ
QQARVEELRHAKYERILETNPGIKLLRGFARFEDAKTLVVRQPDDTEKRLTPDRILIAPGRSPRIPDVPGLAGTPFWTSS
EALIATETPAHLIVHGGSVVALELAQAFLRLGSRVTLVARGKLLSREDPAIGEGLNAVLEEEGMRVLTDTDVKAVRFDGT
TFELETSAGTLSGDRLLVATGRKPNTAGLALDRTGVTTDTSGAIVVDDHLRTSVPHIYAAGDCTNAPQFVYVAAAAGTRA
AINMTGGDAALDLSVVPAVVFTDPQVATVGLTEAQANKLGMETDSRTLTLDNVPRALANFDTRGFIKLVSEKKTGRLLGA
QVLAGEGGEIIQTAALAIQNRMTVADLAGQLFPYITMGEGLKLCAQTFTTDVKQLSCCAG

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=465, Percent_Identity=29.6774193548387, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=32.8976034858388, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI22035672, Length=432, Percent_Identity=30.5555555555556, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI33519430, Length=467, Percent_Identity=26.9807280513919, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519428, Length=467, Percent_Identity=26.9807280513919, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519426, Length=467, Percent_Identity=26.9807280513919, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI148277065, Length=467, Percent_Identity=26.9807280513919, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI148277071, Length=466, Percent_Identity=27.0386266094421, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI291045266, Length=431, Percent_Identity=25.0580046403712, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI291045268, Length=320, Percent_Identity=26.875, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786307, Length=476, Percent_Identity=31.0924369747899, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=30.5309734513274, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1789915, Length=349, Percent_Identity=37.8223495702006, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=27.8969957081545, Blast_Score=165, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=30.4721030042918, Blast_Score=206, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI71983419, Length=349, Percent_Identity=32.0916905444126, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=349, Percent_Identity=32.0916905444126, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17557007, Length=460, Percent_Identity=27.8260869565217, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=432, Percent_Identity=23.1481481481481, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321091, Length=469, Percent_Identity=29.4243070362473, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=28.2327586206897, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=26.0042283298097, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21358499, Length=484, Percent_Identity=30.3719008264463, Blast_Score=205, Evalue=7e-53,
Organism=Drosophila melanogaster, GI17737741, Length=502, Percent_Identity=28.6852589641434, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24640551, Length=513, Percent_Identity=27.4853801169591, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24640553, Length=508, Percent_Identity=27.1653543307087, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640549, Length=501, Percent_Identity=27.3453093812375, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 56657; Mature: 56525

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQILRVTGMTCDHCAASVKQALLRVAGVTRADVSCPDAMARVEVGVDTPVAALLNAVR
CCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHHHHHH
ARGYGAEVGGDATSKASTKLNVTIIGAGSAGFAAAIRAAEEGAEVTVIEASTIGGTCVNV
HCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCEEEEE
GCVPSKILIRAAHVAHLQARHPFPGLVRHAPLLDRTALVAQQQARVEELRHAKYERILET
CCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPGIKLLRGFARFEDAKTLVVRQPDDTEKRLTPDRILIAPGRSPRIPDVPGLAGTPFWTS
CCCHHHHHHHHHHCCCCEEEEECCCCCHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
SEALIATETPAHLIVHGGSVVALELAQAFLRLGSRVTLVARGKLLSREDPAIGEGLNAVL
CCEEEEECCCCEEEEECCCEEHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHH
EEEGMRVLTDTDVKAVRFDGTTFELETSAGTLSGDRLLVATGRKPNTAGLALDRTGVTTD
HHCCCEEEECCCCEEEEECCEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCC
TSGAIVVDDHLRTSVPHIYAAGDCTNAPQFVYVAAAAGTRAAINMTGGDAALDLSVVPAV
CCCEEEECCCHHCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEEEEEEE
VFTDPQVATVGLTEAQANKLGMETDSRTLTLDNVPRALANFDTRGFIKLVSEKKTGRLLG
EECCCCEEEEECCHHHHHHCCCCCCCCEEEECCHHHHHHCCCCCCCCEEECCCCCCCEEE
AQVLAGEGGEIIQTAALAIQNRMTVADLAGQLFPYITMGEGLKLCAQTFTTDVKQLSCCA
EEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCC
G
C
>Mature Secondary Structure 
SEQILRVTGMTCDHCAASVKQALLRVAGVTRADVSCPDAMARVEVGVDTPVAALLNAVR
CCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHHHHHH
ARGYGAEVGGDATSKASTKLNVTIIGAGSAGFAAAIRAAEEGAEVTVIEASTIGGTCVNV
HCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCEEEEE
GCVPSKILIRAAHVAHLQARHPFPGLVRHAPLLDRTALVAQQQARVEELRHAKYERILET
CCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPGIKLLRGFARFEDAKTLVVRQPDDTEKRLTPDRILIAPGRSPRIPDVPGLAGTPFWTS
CCCHHHHHHHHHHCCCCEEEEECCCCCHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
SEALIATETPAHLIVHGGSVVALELAQAFLRLGSRVTLVARGKLLSREDPAIGEGLNAVL
CCEEEEECCCCEEEEECCCEEHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHH
EEEGMRVLTDTDVKAVRFDGTTFELETSAGTLSGDRLLVATGRKPNTAGLALDRTGVTTD
HHCCCEEEECCCCEEEEECCEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCC
TSGAIVVDDHLRTSVPHIYAAGDCTNAPQFVYVAAAAGTRAAINMTGGDAALDLSVVPAV
CCCEEEECCCHHCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEEEEEEE
VFTDPQVATVGLTEAQANKLGMETDSRTLTLDNVPRALANFDTRGFIKLVSEKKTGRLLG
EECCCCEEEEECCHHHHHHCCCCCCCCEEEECCHHHHHHCCCCCCCCEEECCCCCCCEEE
AQVLAGEGGEIIQTAALAIQNRMTVADLAGQLFPYITMGEGLKLCAQTFTTDVKQLSCCA
EEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9167257 [H]