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The map label for this gene is dapE

Identifier: 74317289

GI number: 74317289

Start: 1336981

End: 1338159

Strand: Reverse

Name: dapE

Synonym: Tbd_1271

Alternate gene names: 74317289

Gene position: 1338159-1336981 (Counterclockwise)

Preceding gene: 74317290

Following gene: 74317288

Centisome position: 45.99

GC content: 65.9

Gene sequence:

>1179_bases
ATGGCCAATGAAACGCTCGAGGATGCCGTCCTTGCCAGTGAAACCTTGGCGCTCGCGGTCGAACTGCTCAAGCGCCGCTC
GGTCACGCCCGACGACGCCGGCTGCCACGACCTGATCGCGGCGCGGCTGCAAGCGCTTGGCTTCCACATCGAGCGCCATC
GCCACAACGACGTCGACAATCTGTGGGCGCGTCGCGGCACGGCCTCCCCGGTCGTGTGCTTCGCCGGCCATACCGACGTC
GTCCCGCCCGGGCCGCTCGAGCAGTGGCTCTCCGATCCTTTCGAGCCGACGCTGCGCGACGGCAAGCTCTACGCCCGCGG
CGCCGCCGACATGAAAACCTCGGATGCGGCCTTCGTCACTGCGACCGAGCGCTTCCTCGCCCGCCGTCCCGACCATCCGG
GCTCGATCGCTTTCCTGCTGACTTCCGACGAGGAAGGTCCGGCGACCGACGGCACGGTGCGCGTCGTCGAAGCGCTCAAG
GCACGCGGCGAACTGCTCGACTATTGCATCGTCGGCGAGCCGACGAGCGCCGCCGAATTCGGCGACACGATCAAGAACGG
ACGGCGCGGCTCGCTCTCGGGCACCCTGCGCGTGAAGGGTGTACAGGGGCACATCGCCTACCCCCACCTCGCGAAAAACC
CGATCCATCTCGCGGCGCCGGCGATCGCCGAGCTCGCCGAGACGATGTGGGACGAAGGCAATAGCTACTTCCCGCCGACG
ACCTGGCAGATTTCCAACATCCATGCCGGCACCGGCGTCACCAACGTGATTCCGGGCATGGTCGAGATCCAGTTCAATTT
CCGCTTTTCCACGGCCAGCACGGCCGAGGGCCTGATGGACGCCGTCAACGAAATCCTCGACAGCCACGGCCTCGACTACG
AAATCGACTGGAACCTCTCCGGCAAGCCCTTTCTCACGCCGCGCGGCGCCTTGTGCGACAGGCTCTCCGAAGCCGTCCAC
GAGGTCACCGGGCTCACGCCCGAGCTTTCGACCACGGGCGGCACGTCGGACGGCCGCTTCATCGCCGACATCTGTCGCGA
AGTCGTCGAGTTCGGCCCGCTCAACATGAGCATCCACAAGCTGAACGAGCACGTCGCACTCGAAAACGTCGAGCAGCTCG
CAGCGGTTTACGAGAAGGCGCTGGAAAAGCTGCTGGGCGAGAGCGATGAACGCGTTTGA

Upstream 100 bases:

>100_bases
TCCGAGCGTGATCAAGCGGCCCGTGCTGGAACGCGACGGGCGCTACCATCTCGGCTTCAGCGAAGACTATTACCGCAATC
TTTTCGGAGCATGACCCGGC

Downstream 100 bases:

>100_bases
CGACACCGAATCGCTGATCACGGTCCGCGACTGGCTGCGCTTCGCCGTATCGCGCTTCAACGAGGCCAGGCTCTTCTTCG
GCCATGGCTCGGACAATGCC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MANETLEDAVLASETLALAVELLKRRSVTPDDAGCHDLIAARLQALGFHIERHRHNDVDNLWARRGTASPVVCFAGHTDV
VPPGPLEQWLSDPFEPTLRDGKLYARGAADMKTSDAAFVTATERFLARRPDHPGSIAFLLTSDEEGPATDGTVRVVEALK
ARGELLDYCIVGEPTSAAEFGDTIKNGRRGSLSGTLRVKGVQGHIAYPHLAKNPIHLAAPAIAELAETMWDEGNSYFPPT
TWQISNIHAGTGVTNVIPGMVEIQFNFRFSTASTAEGLMDAVNEILDSHGLDYEIDWNLSGKPFLTPRGALCDRLSEAVH
EVTGLTPELSTTGGTSDGRFIADICREVVEFGPLNMSIHKLNEHVALENVEQLAAVYEKALEKLLGESDERV

Sequences:

>Translated_392_residues
MANETLEDAVLASETLALAVELLKRRSVTPDDAGCHDLIAARLQALGFHIERHRHNDVDNLWARRGTASPVVCFAGHTDV
VPPGPLEQWLSDPFEPTLRDGKLYARGAADMKTSDAAFVTATERFLARRPDHPGSIAFLLTSDEEGPATDGTVRVVEALK
ARGELLDYCIVGEPTSAAEFGDTIKNGRRGSLSGTLRVKGVQGHIAYPHLAKNPIHLAAPAIAELAETMWDEGNSYFPPT
TWQISNIHAGTGVTNVIPGMVEIQFNFRFSTASTAEGLMDAVNEILDSHGLDYEIDWNLSGKPFLTPRGALCDRLSEAVH
EVTGLTPELSTTGGTSDGRFIADICREVVEFGPLNMSIHKLNEHVALENVEQLAAVYEKALEKLLGESDERV
>Mature_391_residues
ANETLEDAVLASETLALAVELLKRRSVTPDDAGCHDLIAARLQALGFHIERHRHNDVDNLWARRGTASPVVCFAGHTDVV
PPGPLEQWLSDPFEPTLRDGKLYARGAADMKTSDAAFVTATERFLARRPDHPGSIAFLLTSDEEGPATDGTVRVVEALKA
RGELLDYCIVGEPTSAAEFGDTIKNGRRGSLSGTLRVKGVQGHIAYPHLAKNPIHLAAPAIAELAETMWDEGNSYFPPTT
WQISNIHAGTGVTNVIPGMVEIQFNFRFSTASTAEGLMDAVNEILDSHGLDYEIDWNLSGKPFLTPRGALCDRLSEAVHE
VTGLTPELSTTGGTSDGRFIADICREVVEFGPLNMSIHKLNEHVALENVEQLAAVYEKALEKLLGESDERV

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Homo sapiens, GI21071039, Length=132, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=54.6666666666667, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1790395, Length=258, Percent_Identity=29.0697674418605, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17551016, Length=275, Percent_Identity=27.6363636363636, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_THIDA (Q3SJE0)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315029.1
- ProteinModelPortal:   Q3SJE0
- SMR:   Q3SJE0
- STRING:   Q3SJE0
- GeneID:   3673361
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1271
- NMPDR:   fig|292415.3.peg.1291
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- PhylomeDB:   Q3SJE0
- ProtClustDB:   PRK13009
- BioCyc:   TDEN292415:TBD_1271-MONOMER
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 42539; Mature: 42408

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 79-79 ACT_SITE 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANETLEDAVLASETLALAVELLKRRSVTPDDAGCHDLIAARLQALGFHIERHRHNDVDN
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
LWARRGTASPVVCFAGHTDVVPPGPLEQWLSDPFEPTLRDGKLYARGAADMKTSDAAFVT
HHHHCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEH
ATERFLARRPDHPGSIAFLLTSDEEGPATDGTVRVVEALKARGELLDYCIVGEPTSAAEF
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHH
GDTIKNGRRGSLSGTLRVKGVQGHIAYPHLAKNPIHLAAPAIAELAETMWDEGNSYFPPT
HHHHHCCCCCCCCCEEEEEEECCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
TWQISNIHAGTGVTNVIPGMVEIQFNFRFSTASTAEGLMDAVNEILDSHGLDYEIDWNLS
CEEEECEECCCCHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
GKPFLTPRGALCDRLSEAVHEVTGLTPELSTTGGTSDGRFIADICREVVEFGPLNMSIHK
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHH
LNEHVALENVEQLAAVYEKALEKLLGESDERV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ANETLEDAVLASETLALAVELLKRRSVTPDDAGCHDLIAARLQALGFHIERHRHNDVDN
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
LWARRGTASPVVCFAGHTDVVPPGPLEQWLSDPFEPTLRDGKLYARGAADMKTSDAAFVT
HHHHCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEH
ATERFLARRPDHPGSIAFLLTSDEEGPATDGTVRVVEALKARGELLDYCIVGEPTSAAEF
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHH
GDTIKNGRRGSLSGTLRVKGVQGHIAYPHLAKNPIHLAAPAIAELAETMWDEGNSYFPPT
HHHHHCCCCCCCCCEEEEEEECCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
TWQISNIHAGTGVTNVIPGMVEIQFNFRFSTASTAEGLMDAVNEILDSHGLDYEIDWNLS
CEEEECEECCCCHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
GKPFLTPRGALCDRLSEAVHEVTGLTPELSTTGGTSDGRFIADICREVVEFGPLNMSIHK
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHH
LNEHVALENVEQLAAVYEKALEKLLGESDERV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA