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The map label for this gene is tpiA

Identifier: 74317158

GI number: 74317158

Start: 1195661

End: 1196407

Strand: Direct

Name: tpiA

Synonym: Tbd_1140

Alternate gene names: 74317158

Gene position: 1195661-1196407 (Clockwise)

Preceding gene: 74317157

Following gene: 74317159

Centisome position: 41.09

GC content: 66.27

Gene sequence:

>747_bases
ATGCGCAAGAAGCTCGTAGCCGGTAACTGGAAAATGCACGGCAGCCTCGCCGAGAACGCGGCGCTGCTCGCCGCGCTCAA
ACCCGCTCTGCAGGGGATCGAGGCTGCGGTCTGCGTGCCGTTTCCCTATCTGGCACAGGTGCAGACCGCGCTGGCAGGCT
CGTCGCTTGCGTGGGGCGCACAAAACGTTTCCGAGCATGGCAAGGGTGCATTTACCGGTGAAGTGTCGGCGTCAATGCTG
CTCGATTTCGGCTGCAAGTACGTCATCGTCGGCCATTCCGAGCGGCGCAGCCTCTATGGCGAGTCGGACGCGCTCGTGGC
CGCGAAATACATGGCTGCGCAGGCCGCCGGGCTCACGCCGATCCTCTGCGTCGGCGAATCGCTCGCCGAGCGTGAGTCGG
GCGTCACCGAGCAGGTCGTCGCGCGCCAGCTCGACGCCGTGGTCGCCGCCGCCGGCATCGCATCGTTGGCCCGGGCGGTC
GTCGCGTACGAGCCCGTCTGGGCGATCGGAACGGGCAAGACCGCGAGCCCCGAACAGGCGCAGGCCGTGCACGCGTTCAT
TCGCGGCAAGCTCGCCGACCTCGATGCTGCGGTCGCAGCCGGCCTGATCATTCAGTATGGGGGTAGCGTAAAGGCCGCAA
ATGCGGCAGAATTGATGGCTCAGCCGGATATCGACGGCGGGCTGATCGGCGGCGCCTCGCTGCTTGCCGATGAGTTCGTG
GCAATCTGTCGGGCGGCTGCCAAATAA

Upstream 100 bases:

>100_bases
GCCCGGCAAGAAGCAGACCGAAGACTACATCACCGGTCGTTTCGGTTGACCGCGTCACGCGTAGCGCTTACCATCGCGCA
CTTTTGTTCGGCAGACATCC

Downstream 100 bases:

>100_bases
GAGTTAGCACATGGAAACGCTGGTCTGGGTCCTTCACGTAATCGTCGCCATCGCCGTCATTGCACTGGTTCTGTTGCAGC
ACGGCAAAGGCGCCGATATG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRKKLVAGNWKMHGSLAENAALLAALKPALQGIEAAVCVPFPYLAQVQTALAGSSLAWGAQNVSEHGKGAFTGEVSASML
LDFGCKYVIVGHSERRSLYGESDALVAAKYMAAQAAGLTPILCVGESLAERESGVTEQVVARQLDAVVAAAGIASLARAV
VAYEPVWAIGTGKTASPEQAQAVHAFIRGKLADLDAAVAAGLIIQYGGSVKAANAAELMAQPDIDGGLIGGASLLADEFV
AICRAAAK

Sequences:

>Translated_248_residues
MRKKLVAGNWKMHGSLAENAALLAALKPALQGIEAAVCVPFPYLAQVQTALAGSSLAWGAQNVSEHGKGAFTGEVSASML
LDFGCKYVIVGHSERRSLYGESDALVAAKYMAAQAAGLTPILCVGESLAERESGVTEQVVARQLDAVVAAAGIASLARAV
VAYEPVWAIGTGKTASPEQAQAVHAFIRGKLADLDAAVAAGLIIQYGGSVKAANAAELMAQPDIDGGLIGGASLLADEFV
AICRAAAK
>Mature_248_residues
MRKKLVAGNWKMHGSLAENAALLAALKPALQGIEAAVCVPFPYLAQVQTALAGSSLAWGAQNVSEHGKGAFTGEVSASML
LDFGCKYVIVGHSERRSLYGESDALVAAKYMAAQAAGLTPILCVGESLAERESGVTEQVVARQLDAVVAAAGIASLARAV
VAYEPVWAIGTGKTASPEQAQAVHAFIRGKLADLDAAVAAGLIIQYGGSVKAANAAELMAQPDIDGGLIGGASLLADEFV
AICRAAAK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=249, Percent_Identity=48.1927710843374, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI4507645, Length=249, Percent_Identity=48.1927710843374, Blast_Score=195, Evalue=3e-50,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=54.2168674698795, Blast_Score=266, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=50.2024291497976, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6320255, Length=247, Percent_Identity=42.914979757085, Blast_Score=178, Evalue=6e-46,
Organism=Drosophila melanogaster, GI28572008, Length=250, Percent_Identity=53.6, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI28572006, Length=250, Percent_Identity=53.6, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI28572004, Length=250, Percent_Identity=53.6, Blast_Score=221, Evalue=5e-58,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_THIDA (Q3SJQ7)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314898.1
- HSSP:   P36204
- ProteinModelPortal:   Q3SJQ7
- SMR:   Q3SJQ7
- STRING:   Q3SJQ7
- GeneID:   3673211
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1140
- NMPDR:   fig|292415.3.peg.1139
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q3SJQ7
- ProtClustDB:   PRK00042
- BioCyc:   TDEN292415:TBD_1140-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 25274; Mature: 25274

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 92-92 ACT_SITE 164-164 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKLVAGNWKMHGSLAENAALLAALKPALQGIEAAVCVPFPYLAQVQTALAGSSLAWGA
CCCCEECCCCEECCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCH
QNVSEHGKGAFTGEVSASMLLDFGCKYVIVGHSERRSLYGESDALVAAKYMAAQAAGLTP
HHHHHCCCCCEECHHHHHHHHHCCCCEEEEECCHHHHHCCCCCHHHHHHHHHHHHCCCCH
ILCVGESLAERESGVTEQVVARQLDAVVAAAGIASLARAVVAYEPVWAIGTGKTASPEQA
HHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHH
QAVHAFIRGKLADLDAAVAAGLIIQYGGSVKAANAAELMAQPDIDGGLIGGASLLADEFV
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHH
AICRAAAK
HHHHHHCC
>Mature Secondary Structure
MRKKLVAGNWKMHGSLAENAALLAALKPALQGIEAAVCVPFPYLAQVQTALAGSSLAWGA
CCCCEECCCCEECCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCH
QNVSEHGKGAFTGEVSASMLLDFGCKYVIVGHSERRSLYGESDALVAAKYMAAQAAGLTP
HHHHHCCCCCEECHHHHHHHHHCCCCEEEEECCHHHHHCCCCCHHHHHHHHHHHHCCCCH
ILCVGESLAERESGVTEQVVARQLDAVVAAAGIASLARAVVAYEPVWAIGTGKTASPEQA
HHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHH
QAVHAFIRGKLADLDAAVAAGLIIQYGGSVKAANAAELMAQPDIDGGLIGGASLLADEFV
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHH
AICRAAAK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA