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The map label for this gene is pleD [H]

Identifier: 74317057

GI number: 74317057

Start: 1102188

End: 1103369

Strand: Reverse

Name: pleD [H]

Synonym: Tbd_1039

Alternate gene names: 74317057

Gene position: 1103369-1102188 (Counterclockwise)

Preceding gene: 74317058

Following gene: 74317052

Centisome position: 37.92

GC content: 65.06

Gene sequence:

>1182_bases
ATGGCCGCGCGCGCGATCCGCTGGGACGAAGCGCTGCCGGAGGCCGCCGGCGTCACGCCGCTCCTGCTTCTCGACATGTC
GGGCTCGCCACGACCCGAGTGGACGGCGCGCCTTCAGAAGCTGCGCCAGCGCTTTCCGACCGGCCAGCTCATTTGCCTCG
AAGTCGGCTCCGACTTCGACCAGTTGCAGCAGGCCCTGCGCGGCGGCTGCGACAGTTGCCTGCTCGAGGGGACGCCTTCG
CACGCCATCGTCGAGCATATCCTCGAACTGAACGAGCGGCAGGAGCAGGAAACCTTCCGCGTGCTGATCGTGGAGGACTC
GAAGACCGCGAGCCACATGATCCAGCGCACGCTGGCCGAGAGCCAGATCGCGTCGGAGATCGTCAACGACCCGCGCCAGA
CCTTGAACGCTCTGAAGCAGTTCAACCCCGACCTGATCCTGATGGACATGTACATGCCGGATTGCACCGGCGTCGAGGTC
GCGCGCATCATCCGTCAGCACGACGAGTTTCTCGGCATGCCCATCGTCTACCTCTCGGGCGAGACGAACGTCGCGCTGCA
GGTCGACGCGATGCGCCTCGGCGGCGATCACTTCCTCACCAAGCCGTTCAACCCGGTTTTCCTCAACACGATAGTCAAGA
GCAAGATCGAGCGCTATCGCGCCTTGCGCCGTTCGATGTACCACGACAGCCTGACCGGCCTGCTCAACCACTCAAGCGGG
AAGAACACGCTCGACATGGTGTTGTCGGGAATCGCGCATGAGGAAGGCACGCTGTCGGTGGTCATGATCGACATCGACCA
CTTCAAGCAGATCAACGACAGCTACGGCCATCCGACCGGCGATCAGGTGATCCGCAGCCTGTCGTGGCTGCTCAAGCAGC
GACTGCGCAAGCACGACATCCTCTGCCGCTACGGCGGCGAGGAGTTCCTCGTCGGGCTGCCGCACACCGACGCGGGCCAG
GCGCTCGCGATCCTCGAGCGCGTGCGCCAGGATTTCGCGCAGATTCGCCACCCCTATCGGGACACCCATTTCAGCGCCAG
CGTCAGCGCCGGCGTCGCGAGTTATCCGCACCAGCAGAGCGGCGACGCGCTGATCAAGGCCGCCGACGAAGCGCTCTACC
AGGCCAAGCACCGCGGCCGCAATCGCGTCCACATCGGCATGCCTGCACAGGCCCTGGCCTAG

Upstream 100 bases:

>100_bases
CGACCCGCAGTAGGATGCCGATCGCGTCGACCAGACCTGGCTGATCACGGCCGACGCTTCGGCGTGGTCGGCGCTGCAGG
CGCAGCTCGGGTATTTCGGC

Downstream 100 bases:

>100_bases
CCGAGAATCAGCTTCAAGCCGATCAGTATCGTCACGGTTTCGAACGCGCGCTTGATCCACAAATTGCCCTTGCGGATCGC
CAGATGGCTGCCGAGATAAC

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MAARAIRWDEALPEAAGVTPLLLLDMSGSPRPEWTARLQKLRQRFPTGQLICLEVGSDFDQLQQALRGGCDSCLLEGTPS
HAIVEHILELNERQEQETFRVLIVEDSKTASHMIQRTLAESQIASEIVNDPRQTLNALKQFNPDLILMDMYMPDCTGVEV
ARIIRQHDEFLGMPIVYLSGETNVALQVDAMRLGGDHFLTKPFNPVFLNTIVKSKIERYRALRRSMYHDSLTGLLNHSSG
KNTLDMVLSGIAHEEGTLSVVMIDIDHFKQINDSYGHPTGDQVIRSLSWLLKQRLRKHDILCRYGGEEFLVGLPHTDAGQ
ALAILERVRQDFAQIRHPYRDTHFSASVSAGVASYPHQQSGDALIKAADEALYQAKHRGRNRVHIGMPAQALA

Sequences:

>Translated_393_residues
MAARAIRWDEALPEAAGVTPLLLLDMSGSPRPEWTARLQKLRQRFPTGQLICLEVGSDFDQLQQALRGGCDSCLLEGTPS
HAIVEHILELNERQEQETFRVLIVEDSKTASHMIQRTLAESQIASEIVNDPRQTLNALKQFNPDLILMDMYMPDCTGVEV
ARIIRQHDEFLGMPIVYLSGETNVALQVDAMRLGGDHFLTKPFNPVFLNTIVKSKIERYRALRRSMYHDSLTGLLNHSSG
KNTLDMVLSGIAHEEGTLSVVMIDIDHFKQINDSYGHPTGDQVIRSLSWLLKQRLRKHDILCRYGGEEFLVGLPHTDAGQ
ALAILERVRQDFAQIRHPYRDTHFSASVSAGVASYPHQQSGDALIKAADEALYQAKHRGRNRVHIGMPAQALA
>Mature_392_residues
AARAIRWDEALPEAAGVTPLLLLDMSGSPRPEWTARLQKLRQRFPTGQLICLEVGSDFDQLQQALRGGCDSCLLEGTPSH
AIVEHILELNERQEQETFRVLIVEDSKTASHMIQRTLAESQIASEIVNDPRQTLNALKQFNPDLILMDMYMPDCTGVEVA
RIIRQHDEFLGMPIVYLSGETNVALQVDAMRLGGDHFLTKPFNPVFLNTIVKSKIERYRALRRSMYHDSLTGLLNHSSGK
NTLDMVLSGIAHEEGTLSVVMIDIDHFKQINDSYGHPTGDQVIRSLSWLLKQRLRKHDILCRYGGEEFLVGLPHTDAGQA
LAILERVRQDFAQIRHPYRDTHFSASVSAGVASYPHQQSGDALIKAADEALYQAKHRGRNRVHIGMPAQALA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=38.2716049382716, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=39.0532544378698, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=31.3609467455621, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=33.9181286549708, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=34.6590909090909, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=33.1428571428571, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=35.1190476190476, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI87081974, Length=234, Percent_Identity=28.6324786324786, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787802, Length=130, Percent_Identity=33.8461538461538, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081977, Length=183, Percent_Identity=30.0546448087432, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=32.0987654320988, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787056, Length=160, Percent_Identity=30, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=129, Percent_Identity=26.3565891472868, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788394, Length=176, Percent_Identity=25.5681818181818, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788956, Length=161, Percent_Identity=29.1925465838509, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44057; Mature: 43926

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARAIRWDEALPEAAGVTPLLLLDMSGSPRPEWTARLQKLRQRFPTGQLICLEVGSDFD
CCCCCCCHHHHCCHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHH
QLQQALRGGCDSCLLEGTPSHAIVEHILELNERQEQETFRVLIVEDSKTASHMIQRTLAE
HHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHH
SQIASEIVNDPRQTLNALKQFNPDLILMDMYMPDCTGVEVARIIRQHDEFLGMPIVYLSG
HHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECC
ETNVALQVDAMRLGGDHFLTKPFNPVFLNTIVKSKIERYRALRRSMYHDSLTGLLNHSSG
CCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KNTLDMVLSGIAHEEGTLSVVMIDIDHFKQINDSYGHPTGDQVIRSLSWLLKQRLRKHDI
CHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCE
LCRYGGEEFLVGLPHTDAGQALAILERVRQDFAQIRHPYRDTHFSASVSAGVASYPHQQS
EEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHCCCCCCC
GDALIKAADEALYQAKHRGRNRVHIGMPAQALA
CCHHHHHHHHHHHHHHHCCCCEEEECCCHHHCC
>Mature Secondary Structure 
AARAIRWDEALPEAAGVTPLLLLDMSGSPRPEWTARLQKLRQRFPTGQLICLEVGSDFD
CCCCCCHHHHCCHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHH
QLQQALRGGCDSCLLEGTPSHAIVEHILELNERQEQETFRVLIVEDSKTASHMIQRTLAE
HHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHH
SQIASEIVNDPRQTLNALKQFNPDLILMDMYMPDCTGVEVARIIRQHDEFLGMPIVYLSG
HHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECC
ETNVALQVDAMRLGGDHFLTKPFNPVFLNTIVKSKIERYRALRRSMYHDSLTGLLNHSSG
CCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KNTLDMVLSGIAHEEGTLSVVMIDIDHFKQINDSYGHPTGDQVIRSLSWLLKQRLRKHDI
CHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCE
LCRYGGEEFLVGLPHTDAGQALAILERVRQDFAQIRHPYRDTHFSASVSAGVASYPHQQS
EEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHCCCCCCC
GDALIKAADEALYQAKHRGRNRVHIGMPAQALA
CCHHHHHHHHHHHHHHHCCCCEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA