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The map label for this gene is pheS

Identifier: 74317029

GI number: 74317029

Start: 1079147

End: 1080181

Strand: Direct

Name: pheS

Synonym: Tbd_1011

Alternate gene names: 74317029

Gene position: 1079147-1080181 (Clockwise)

Preceding gene: 74317028

Following gene: 74317030

Centisome position: 37.09

GC content: 66.18

Gene sequence:

>1035_bases
ATGCGCAACCCCAACGAAATCGTTGCCGAAGCCCTAGCCGCCATCCATGGCTGCCCTGACCTGCCGAGCCTGGAACAGAT
CAAGGCCGGCTACCTCGGCAAGAGCGGCCAGCTCACCGAACTGCTGAAGAGCCTCGGCGCGATGCCGGCGGACGCGCGCA
AGACCGCGGGTGCGCGCATCAACGAAGCCAAGCAGGCCGTCGAGGCGGCGCTCAAGATACGGCGCGAGGCCTTGCAGCAG
GCCGAACTCGACCGCCAGCTCGCGGCCGAGACGCTCGACGTGACCCTGCCCGGCCGCGGACTCGGCACGGCTGGCGTGCA
TCCGGTGTCGCGCACGCTCGCGCGCATCGAGGCGCTGTTCCATTCGATCGGCTTCGACGTGGCGACGGGGCCGGAAATCG
AGACCGATTTCTACAACTTCACCGCGCTCAACATCCCCGAGGATCATCCGGCGCGGGCCATGCACGACACCTTTTATCTG
CGCCAGAACAATGCGGGCGGCGGTGAACTGCTGCGCACGCACACGTCGCCGGTGCAGATCCGGCACATGCAGACGCACGA
GCCGCCGCTGCGCATCATCGCGCCGGGCCGCGTCTACCGCTGCGATTCCGACGTCACCCACACGCCGATGTTTCACCAGA
TCGAGGGCCTGTGGGTCGACGAGTCGGTGTCGTTCGCCGACCTGAAGGGCGTGCTCGCCGACTTCATGCGCAACTTCTTC
GAACGCGACGACCTTGCCGTGCGCTTTCGCCCCTCGTTCTTCCCGTTCACCGAGCCCTCGGCGGAAATGGACATCGGCTG
CGTGATGTGCGGCGGCTCGGGCTGCCGCGTGTGCTCGCACACCGGCTGGCTCGAGGTGCTCGGCTGCGGCATGGTCCACC
CCAACGTCTTCAGGCACGTCGGCGTCGACGCCGAGCGTTTCATCGGCTTCGCCTTCGGCCTCGGCGTCGAGCGCCTCGCG
ATGCTGCGCTACGGCGTCGACGACCTGCGGCTGTTTTTCGAAAATGATCTGCGTTTTCTCAAGCAATTCAATTAG

Upstream 100 bases:

>100_bases
CGATCAGGTCAAGGCCAAGCTGGCGGCGTAAGCCGTCAACGTGTCACAAGAAAAGGCCGCAAGGCCTTTTTTTGTTTGTG
AGTGATTCGAGATTTCCGCC

Downstream 100 bases:

>100_bases
TCGGGCGCGGATCGCTTCGTCGGAGAGTTGTCCTTGCACGGCATGGGCCGGAGCGGTTTCCTGAAGAATTTCAACTAAAT
ACGGCGCACTTATCATGCAA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRNPNEIVAEALAAIHGCPDLPSLEQIKAGYLGKSGQLTELLKSLGAMPADARKTAGARINEAKQAVEAALKIRREALQQ
AELDRQLAAETLDVTLPGRGLGTAGVHPVSRTLARIEALFHSIGFDVATGPEIETDFYNFTALNIPEDHPARAMHDTFYL
RQNNAGGGELLRTHTSPVQIRHMQTHEPPLRIIAPGRVYRCDSDVTHTPMFHQIEGLWVDESVSFADLKGVLADFMRNFF
ERDDLAVRFRPSFFPFTEPSAEMDIGCVMCGGSGCRVCSHTGWLEVLGCGMVHPNVFRHVGVDAERFIGFAFGLGVERLA
MLRYGVDDLRLFFENDLRFLKQFN

Sequences:

>Translated_344_residues
MRNPNEIVAEALAAIHGCPDLPSLEQIKAGYLGKSGQLTELLKSLGAMPADARKTAGARINEAKQAVEAALKIRREALQQ
AELDRQLAAETLDVTLPGRGLGTAGVHPVSRTLARIEALFHSIGFDVATGPEIETDFYNFTALNIPEDHPARAMHDTFYL
RQNNAGGGELLRTHTSPVQIRHMQTHEPPLRIIAPGRVYRCDSDVTHTPMFHQIEGLWVDESVSFADLKGVLADFMRNFF
ERDDLAVRFRPSFFPFTEPSAEMDIGCVMCGGSGCRVCSHTGWLEVLGCGMVHPNVFRHVGVDAERFIGFAFGLGVERLA
MLRYGVDDLRLFFENDLRFLKQFN
>Mature_344_residues
MRNPNEIVAEALAAIHGCPDLPSLEQIKAGYLGKSGQLTELLKSLGAMPADARKTAGARINEAKQAVEAALKIRREALQQ
AELDRQLAAETLDVTLPGRGLGTAGVHPVSRTLARIEALFHSIGFDVATGPEIETDFYNFTALNIPEDHPARAMHDTFYL
RQNNAGGGELLRTHTSPVQIRHMQTHEPPLRIIAPGRVYRCDSDVTHTPMFHQIEGLWVDESVSFADLKGVLADFMRNFF
ERDDLAVRFRPSFFPFTEPSAEMDIGCVMCGGSGCRVCSHTGWLEVLGCGMVHPNVFRHVGVDAERFIGFAFGLGVERLA
MLRYGVDDLRLFFENDLRFLKQFN

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=295, Percent_Identity=33.2203389830508, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4758340, Length=266, Percent_Identity=30.4511278195489, Blast_Score=115, Evalue=8e-26,
Organism=Escherichia coli, GI1788007, Length=339, Percent_Identity=59.2920353982301, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32563657, Length=268, Percent_Identity=28.7313432835821, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=268, Percent_Identity=28.7313432835821, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=254, Percent_Identity=34.251968503937, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=275, Percent_Identity=29.0909090909091, Blast_Score=122, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=101, Percent_Identity=36.6336633663366, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI18858079, Length=279, Percent_Identity=27.5985663082437, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17137424, Length=255, Percent_Identity=30.5882352941176, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_THIDA (Q3SK30)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314769.1
- ProteinModelPortal:   Q3SK30
- SMR:   Q3SK30
- STRING:   Q3SK30
- GeneID:   3671235
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1011
- NMPDR:   fig|292415.3.peg.741
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q3SK30
- ProtClustDB:   PRK00488
- BioCyc:   TDEN292415:TBD_1011-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38135; Mature: 38135

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNPNEIVAEALAAIHGCPDLPSLEQIKAGYLGKSGQLTELLKSLGAMPADARKTAGARI
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHCCCHH
NEAKQAVEAALKIRREALQQAELDRQLAAETLDVTLPGRGLGTAGVHPVSRTLARIEALF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHH
HSIGFDVATGPEIETDFYNFTALNIPEDHPARAMHDTFYLRQNNAGGGELLRTHTSPVQI
HHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHEEEEEECCCCCCCEEEECCCCEEE
RHMQTHEPPLRIIAPGRVYRCDSDVTHTPMFHQIEGLWVDESVSFADLKGVLADFMRNFF
EEECCCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEECCCCCHHHHHHHHHHHHHHHH
ERDDLAVRFRPSFFPFTEPSAEMDIGCVMCGGSGCRVCSHTGWLEVLGCGMVHPNVFRHV
CCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCHHHHHC
GVDAERFIGFAFGLGVERLAMLRYGVDDLRLFFENDLRFLKQFN
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRNPNEIVAEALAAIHGCPDLPSLEQIKAGYLGKSGQLTELLKSLGAMPADARKTAGARI
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHCCCHH
NEAKQAVEAALKIRREALQQAELDRQLAAETLDVTLPGRGLGTAGVHPVSRTLARIEALF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHH
HSIGFDVATGPEIETDFYNFTALNIPEDHPARAMHDTFYLRQNNAGGGELLRTHTSPVQI
HHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHEEEEEECCCCCCCEEEECCCCEEE
RHMQTHEPPLRIIAPGRVYRCDSDVTHTPMFHQIEGLWVDESVSFADLKGVLADFMRNFF
EEECCCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEECCCCCHHHHHHHHHHHHHHHH
ERDDLAVRFRPSFFPFTEPSAEMDIGCVMCGGSGCRVCSHTGWLEVLGCGMVHPNVFRHV
CCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCHHHHHC
GVDAERFIGFAFGLGVERLAMLRYGVDDLRLFFENDLRFLKQFN
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA