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The map label for this gene is radA [H]

Identifier: 74316983

GI number: 74316983

Start: 1027097

End: 1028449

Strand: Direct

Name: radA [H]

Synonym: Tbd_0965

Alternate gene names: 74316983

Gene position: 1027097-1028449 (Clockwise)

Preceding gene: 74316982

Following gene: 74316984

Centisome position: 35.3

GC content: 68.74

Gene sequence:

>1353_bases
ATGGCGAAGGCCAAAACGGTTTACACCTGCACCGAGTGCGGCGGCAGCTCGCCCAAATGGCAGGGCCAGTGCCCGGCGTG
CAACGCCTGGAACACGCTGGTCGAGACGATCGCCGAGACGGCAAAGCCCCGCTTCGCCGCGCTCGCGGCGACGAGTCAGG
TACAGCTGCTGCACGAGGTCGAGGCCTCGGAGGAAAGCCGCATCGCGACAGGGATCGGCGAACTCGACCGCGTCCTGGGC
GGCGGCCTCGTCCCGGGCGGCGTCACGCTGATCGGCGGCGACCCGGGCATCGGAAAATCGACGCTGCTGCTGCAGGCGCT
CGCCGGGCTGGCGGAGCGCATGGGGACGCTCTACGTCAGCGGCGAGGAATCGGCACGTCAGGTCGCCTTGCGCGCCCGGC
GTCTTGCCCTGCCCGAAACGCGGCTGCCGGTTTTGCCCGAGATTCGGCTCGAGGCGATCCTCGCGCAGCTCGCGGCGACC
TCGCCCGAGGTCGCCGTCGTCGACTCGATCCAGACCGTTTATTCGGAGGCCCTGACCTCGGCGCCGGGATCGGTCGCGCA
GGTGCGCGAGTGCGCGGCGCAGCTCACCCGCCACGCCAAGCAGAGCGGCACCGCGGTCATCCTGGTCGGGCACGTCACCA
AGGAAGGCGCGATCGCCGGGCCGCGCGTGCTCGAGCACATTGTCGACACCGTGTTGTACTTCGAGGGGGACACGGGCTCG
AGCTACCGCCTCGTGCGCGCGTTCAAGAACCGCTTCGGGGCGGTCAACGAGATCGGCGTGTTCGCGATGACCGAAAAGGG
CCTGAAGGGCGTTTCGAACCCCTCGGCAATCTTTCTCTCGCGTCACGGCGAGCCCGTGCCGGGTTCGTGCGTGCTCGTGA
CGCAGGAAGGAACGCGCCCGCTCCTCGTCGAGATCCAGGCGCTCGTCGACACGAGTCACGCGCCCAATCCACGCCGTCTG
AGTGTCGGCCTCGAGCAGAACCGTCTCGCGATGCTACTCGCCGTCTTGCATCGCCACGCCGGCATCGCCTGTTTCGATCA
GGACGTCTTCGTCAACGCGGTCGGCGGGGTGAAGATCAGCGAGCCGGCGGCCGACCTCGCCGTCCTGGCCGCGATCGTGT
CGTCGCTTAGGAGCCAGCCGCTGCCCGACAAGCTCGTGATGTTCGGCGAAGTGGGGCTCGCCGGCGAGATCCGTCCGGTG
CAGCGCGGACAGGAGCGCCTGAAGGAAGCGGCCAAGCTCGGCTTCACCCAGGCCATCGTGCCGGCCGCGAACCAGCCCAA
GCAGAAAATCGCCGGCATGACGGTGCACGCGGTGCAGCGTCTCGACGACGCGATCAGCCTGTTCCGCGGCTAG

Upstream 100 bases:

>100_bases
AACTGTCGAAGGAAGTCGAGAACGCGACCATCGTCTCGATCGTCTGCGACCGCGGCGACCGCTATCTGTCGACCGGCGTG
TTCCCCGCGTAAGCGGCGTC

Downstream 100 bases:

>100_bases
CGGTCATGCCTGCGCGGAGCGTTTCGGCCCGCAGCCGCTGGCTATACTTCGAAGGCGAATCAAACGCGTACGGAGGCTGT
CATGAAGAGATGCGGAAAGG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAKAKTVYTCTECGGSSPKWQGQCPACNAWNTLVETIAETAKPRFAALAATSQVQLLHEVEASEESRIATGIGELDRVLG
GGLVPGGVTLIGGDPGIGKSTLLLQALAGLAERMGTLYVSGEESARQVALRARRLALPETRLPVLPEIRLEAILAQLAAT
SPEVAVVDSIQTVYSEALTSAPGSVAQVRECAAQLTRHAKQSGTAVILVGHVTKEGAIAGPRVLEHIVDTVLYFEGDTGS
SYRLVRAFKNRFGAVNEIGVFAMTEKGLKGVSNPSAIFLSRHGEPVPGSCVLVTQEGTRPLLVEIQALVDTSHAPNPRRL
SVGLEQNRLAMLLAVLHRHAGIACFDQDVFVNAVGGVKISEPAADLAVLAAIVSSLRSQPLPDKLVMFGEVGLAGEIRPV
QRGQERLKEAAKLGFTQAIVPAANQPKQKIAGMTVHAVQRLDDAISLFRG

Sequences:

>Translated_450_residues
MAKAKTVYTCTECGGSSPKWQGQCPACNAWNTLVETIAETAKPRFAALAATSQVQLLHEVEASEESRIATGIGELDRVLG
GGLVPGGVTLIGGDPGIGKSTLLLQALAGLAERMGTLYVSGEESARQVALRARRLALPETRLPVLPEIRLEAILAQLAAT
SPEVAVVDSIQTVYSEALTSAPGSVAQVRECAAQLTRHAKQSGTAVILVGHVTKEGAIAGPRVLEHIVDTVLYFEGDTGS
SYRLVRAFKNRFGAVNEIGVFAMTEKGLKGVSNPSAIFLSRHGEPVPGSCVLVTQEGTRPLLVEIQALVDTSHAPNPRRL
SVGLEQNRLAMLLAVLHRHAGIACFDQDVFVNAVGGVKISEPAADLAVLAAIVSSLRSQPLPDKLVMFGEVGLAGEIRPV
QRGQERLKEAAKLGFTQAIVPAANQPKQKIAGMTVHAVQRLDDAISLFRG
>Mature_449_residues
AKAKTVYTCTECGGSSPKWQGQCPACNAWNTLVETIAETAKPRFAALAATSQVQLLHEVEASEESRIATGIGELDRVLGG
GLVPGGVTLIGGDPGIGKSTLLLQALAGLAERMGTLYVSGEESARQVALRARRLALPETRLPVLPEIRLEAILAQLAATS
PEVAVVDSIQTVYSEALTSAPGSVAQVRECAAQLTRHAKQSGTAVILVGHVTKEGAIAGPRVLEHIVDTVLYFEGDTGSS
YRLVRAFKNRFGAVNEIGVFAMTEKGLKGVSNPSAIFLSRHGEPVPGSCVLVTQEGTRPLLVEIQALVDTSHAPNPRRLS
VGLEQNRLAMLLAVLHRHAGIACFDQDVFVNAVGGVKISEPAADLAVLAAIVSSLRSQPLPDKLVMFGEVGLAGEIRPVQ
RGQERLKEAAKLGFTQAIVPAANQPKQKIAGMTVHAVQRLDDAISLFRG

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=458, Percent_Identity=58.9519650655022, Blast_Score=531, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 47450; Mature: 47319

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKTVYTCTECGGSSPKWQGQCPACNAWNTLVETIAETAKPRFAALAATSQVQLLHEV
CCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EASEESRIATGIGELDRVLGGGLVPGGVTLIGGDPGIGKSTLLLQALAGLAERMGTLYVS
HCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCEEEEC
GEESARQVALRARRLALPETRLPVLPEIRLEAILAQLAATSPEVAVVDSIQTVYSEALTS
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
APGSVAQVRECAAQLTRHAKQSGTAVILVGHVTKEGAIAGPRVLEHIVDTVLYFEGDTGS
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHEEEECCCCC
SYRLVRAFKNRFGAVNEIGVFAMTEKGLKGVSNPSAIFLSRHGEPVPGSCVLVTQEGTRP
CHHHHHHHHHHCCCHHHCCEEEEEHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCC
LLVEIQALVDTSHAPNPRRLSVGLEQNRLAMLLAVLHRHAGIACFDQDVFVNAVGGVKIS
HHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEECCCCEEEEECCEEEC
EPAADLAVLAAIVSSLRSQPLPDKLVMFGEVGLAGEIRPVQRGQERLKEAAKLGFTQAIV
CCHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHCC
PAANQPKQKIAGMTVHAVQRLDDAISLFRG
CCCCCCHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAKTVYTCTECGGSSPKWQGQCPACNAWNTLVETIAETAKPRFAALAATSQVQLLHEV
CCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EASEESRIATGIGELDRVLGGGLVPGGVTLIGGDPGIGKSTLLLQALAGLAERMGTLYVS
HCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCEEEEC
GEESARQVALRARRLALPETRLPVLPEIRLEAILAQLAATSPEVAVVDSIQTVYSEALTS
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
APGSVAQVRECAAQLTRHAKQSGTAVILVGHVTKEGAIAGPRVLEHIVDTVLYFEGDTGS
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHEEEECCCCC
SYRLVRAFKNRFGAVNEIGVFAMTEKGLKGVSNPSAIFLSRHGEPVPGSCVLVTQEGTRP
CHHHHHHHHHHCCCHHHCCEEEEEHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCC
LLVEIQALVDTSHAPNPRRLSVGLEQNRLAMLLAVLHRHAGIACFDQDVFVNAVGGVKIS
HHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEECCCCEEEEECCEEEC
EPAADLAVLAAIVSSLRSQPLPDKLVMFGEVGLAGEIRPVQRGQERLKEAAKLGFTQAIV
CCHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHCC
PAANQPKQKIAGMTVHAVQRLDDAISLFRG
CCCCCCHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]