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The map label for this gene is prfB [H]

Identifier: 74316939

GI number: 74316939

Start: 974461

End: 975510

Strand: Reverse

Name: prfB [H]

Synonym: Tbd_0921

Alternate gene names: 74316939

Gene position: 975510-974461 (Counterclockwise)

Preceding gene: 74316940

Following gene: 74316937

Centisome position: 33.52

GC content: 64.95

Gene sequence:

>1050_bases
GTGCAACCCAGCTCAGGGGGTTTCTTTGACTACCCTGTCAAGAAAGACCGCCTAGAAGAAGTCGTCCGCCTGTCCGAAGC
GCCCGATTTCTGGAACGACGCCGCGCGCGCCCAGGAACTCGGGCGCGAACGCAAGTCGCTCGAGGACGTCGTCCTCGTGC
TCGACCGCGTGACCTCGGGCCTCAAGGACGCCGGTGAACTGTTCGAGATGGCGCGCGAGGAGAACGACGACGACACGCTC
GCGGCGGTGCAGGCCGACATCGACGGCCTCGAGAAGGACGTCGCGACGCTCGAGTTCCGCCGCATGTTCAGCAACCCGGC
GGACCCGTCCAACTGCTTCCTCGACATCCAGGCCGGCGCGGGCGGCACCGAGGCCTGCGACTGGGCGTCGATGCTGTTGC
GGCAATATCTCAAATATGCCGAGCGCAAGGGCTTCAAGGCCGAGCTGCTCGAGGAATCCGAAGGCGACGTCGCCGGCATC
AAGTCGGCGTCGATCAAGATCGAGGGCGACTACGCCTACGGCTACCTGCGCACCGAGACCGGCGTGCACCGCCTCGTGCG
CAAGTCGCCGTTCGACTCCTCGGGCGGGCGTCACACCTCGTTCGCGAGCGTGTTCGTCTATCCCGAGGTCGACGACTCGA
TCGAAGTCGAGATCAATCCGGCCGACCTCAGGGTCGACACCTACCGCGCGTCGGGCGCCGGCGGCCAGCACATCAACAAG
ACCGACTCGGCCGTGCGCATCACGCACATTCCGTCGGGCATCGTCGTCCAGTGCCAGAACGACCGCTCGCAGCACAAGAA
CCGCGCCGAGGCGATGTCGATGCTGAAGTCGAAGCTCTACGAAGCCGAGCTGCGCAAGCGCCAGGCCGAACAGGACAAGC
TCGAAGCCGGCAAGGCCGACGTCGGCTGGGGCCACCAGATCCGCTCCTACGTGCTCGACAATTCGCGGATCAAGGACCTG
CGCACCAACGTCGAAGTCTCCGCGACGCAGAAAGTGCTCGACGGCGACCTCGACGTGTTCATCGAAGCAAGCCTCAAACA
AGGCGTCTAG

Upstream 100 bases:

>100_bases
GTGGGCTATAATGCGCGGTTTTTCCGCCTAGTTACGCGCACAATCATGGAAGCCGAAAGCCTCAATCAGATCGAAGCCAA
ACTCGCCGACTTGAGCGAGC

Downstream 100 bases:

>100_bases
GCGATCGCCCTACCCGCAGCCCGGCCTCAAAGCCGGGCCGCGGCCTGAATCAGGTCGACGAGAAACACCAGCAGGCCGAG
CGTGATGAAGGCATAGAGCA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MQPSSGGFFDYPVKKDRLEEVVRLSEAPDFWNDAARAQELGRERKSLEDVVLVLDRVTSGLKDAGELFEMAREENDDDTL
AAVQADIDGLEKDVATLEFRRMFSNPADPSNCFLDIQAGAGGTEACDWASMLLRQYLKYAERKGFKAELLEESEGDVAGI
KSASIKIEGDYAYGYLRTETGVHRLVRKSPFDSSGGRHTSFASVFVYPEVDDSIEVEINPADLRVDTYRASGAGGQHINK
TDSAVRITHIPSGIVVQCQNDRSQHKNRAEAMSMLKSKLYEAELRKRQAEQDKLEAGKADVGWGHQIRSYVLDNSRIKDL
RTNVEVSATQKVLDGDLDVFIEASLKQGV

Sequences:

>Translated_349_residues
MQPSSGGFFDYPVKKDRLEEVVRLSEAPDFWNDAARAQELGRERKSLEDVVLVLDRVTSGLKDAGELFEMAREENDDDTL
AAVQADIDGLEKDVATLEFRRMFSNPADPSNCFLDIQAGAGGTEACDWASMLLRQYLKYAERKGFKAELLEESEGDVAGI
KSASIKIEGDYAYGYLRTETGVHRLVRKSPFDSSGGRHTSFASVFVYPEVDDSIEVEINPADLRVDTYRASGAGGQHINK
TDSAVRITHIPSGIVVQCQNDRSQHKNRAEAMSMLKSKLYEAELRKRQAEQDKLEAGKADVGWGHQIRSYVLDNSRIKDL
RTNVEVSATQKVLDGDLDVFIEASLKQGV
>Mature_349_residues
MQPSSGGFFDYPVKKDRLEEVVRLSEAPDFWNDAARAQELGRERKSLEDVVLVLDRVTSGLKDAGELFEMAREENDDDTL
AAVQADIDGLEKDVATLEFRRMFSNPADPSNCFLDIQAGAGGTEACDWASMLLRQYLKYAERKGFKAELLEESEGDVAGI
KSASIKIEGDYAYGYLRTETGVHRLVRKSPFDSSGGRHTSFASVFVYPEVDDSIEVEINPADLRVDTYRASGAGGQHINK
TDSAVRITHIPSGIVVQCQNDRSQHKNRAEAMSMLKSKLYEAELRKRQAEQDKLEAGKADVGWGHQIRSYVLDNSRIKDL
RTNVEVSATQKVLDGDLDVFIEASLKQGV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=316, Percent_Identity=36.0759493670886, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI34577120, Length=250, Percent_Identity=38, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI166795305, Length=215, Percent_Identity=35.8139534883721, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=66.1807580174927, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1787462, Length=340, Percent_Identity=38.8235294117647, Blast_Score=216, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=261, Percent_Identity=31.4176245210728, Blast_Score=117, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6321295, Length=242, Percent_Identity=38.8429752066116, Blast_Score=152, Evalue=6e-38,
Organism=Drosophila melanogaster, GI19921226, Length=277, Percent_Identity=38.6281588447653, Blast_Score=164, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 38792; Mature: 38792

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPSSGGFFDYPVKKDRLEEVVRLSEAPDFWNDAARAQELGRERKSLEDVVLVLDRVTSG
CCCCCCCEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKDAGELFEMAREENDDDTLAAVQADIDGLEKDVATLEFRRMFSNPADPSNCFLDIQAGA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
GGTEACDWASMLLRQYLKYAERKGFKAELLEESEGDVAGIKSASIKIEGDYAYGYLRTET
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEEEEEEECCEEEEEEECHH
GVHRLVRKSPFDSSGGRHTSFASVFVYPEVDDSIEVEINPADLRVDTYRASGAGGQHINK
HHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCCCCC
TDSAVRITHIPSGIVVQCQNDRSQHKNRAEAMSMLKSKLYEAELRKRQAEQDKLEAGKAD
CCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VGWGHQIRSYVLDNSRIKDLRTNVEVSATQKVLDGDLDVFIEASLKQGV
CCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCEEEEEEHHHCCC
>Mature Secondary Structure
MQPSSGGFFDYPVKKDRLEEVVRLSEAPDFWNDAARAQELGRERKSLEDVVLVLDRVTSG
CCCCCCCEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKDAGELFEMAREENDDDTLAAVQADIDGLEKDVATLEFRRMFSNPADPSNCFLDIQAGA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
GGTEACDWASMLLRQYLKYAERKGFKAELLEESEGDVAGIKSASIKIEGDYAYGYLRTET
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEEEEEEECCEEEEEEECHH
GVHRLVRKSPFDSSGGRHTSFASVFVYPEVDDSIEVEINPADLRVDTYRASGAGGQHINK
HHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCCCCC
TDSAVRITHIPSGIVVQCQNDRSQHKNRAEAMSMLKSKLYEAELRKRQAEQDKLEAGKAD
CCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VGWGHQIRSYVLDNSRIKDLRTNVEVSATQKVLDGDLDVFIEASLKQGV
CCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCEEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA