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The map label for this gene is metG

Identifier: 74316878

GI number: 74316878

Start: 910688

End: 912760

Strand: Reverse

Name: metG

Synonym: Tbd_0860

Alternate gene names: 74316878

Gene position: 912760-910688 (Counterclockwise)

Preceding gene: 74316879

Following gene: 74316877

Centisome position: 31.37

GC content: 63.87

Gene sequence:

>2073_bases
ATGTCCCGTCAAATCCTCGTCACCTCTGCGCTGCCCTACGCCAACGGCAGCATTCATCTGGGTCATCTGGTCGAGTACAT
CCAGACCGACATTTGGGTGCGTTTCCAGAAAATGCGGGGCACCAACTGCCGCTACATGTGCGCCGACGACACGCACGGCA
CCGCGATCATGCTGCGCGCCGAGAAGGAAGCCATCACACCCGAGGCGCTGATCCAGCGCGTGTGGGCCGAACACACGCGC
GATTTCGCGGGCTTCCACATCGGCTTCGATCACTATTACTCGACGCACTCGGACGAGAACCGCGAACACGCATCGAAGAT
CTACCTGGCCTTGAAGGACGCCGGGCTGATCGAGGTGAAGACGATCGCGCAGCTGTACGACCCGGTGAAGAACCTCTTCC
TGCCCGACCGCTTCATCAAGGGCGAATGCCCGAAGTGCGGCGCGGCGGACCAGTACGGCGACAACTGCGAGGTCTGCGGC
GCGACCTACAGCCCGACCGAGCTCAAGAACCCCGTCTCGGCGGTGTCGGGCGCGACGCCGGTGCACAAGGATTCCGAGCA
CTACTTCTTCAAGCTCGGCGACTGCGAGGCCTTTCTGCGCGAGTGGACCACCTCAGGGACGCTGCAGGCCGAGGCCGCTA
ACAAGATGCAGGAGTGGTTCGCGGCGGGACTCAACAACTGGGACATCAGCCGCGACGCGCCCTACTTCGGCTTCGAGATC
CCCGACGCGCCGGGCAAGTATTTCTACGTCTGGCTCGACGCGCCGGTCGGCTACATGGCGAGCTTCGCCAGATATGCGAA
GGACCACGGCCTCGACTTCGACGCCTGGTGGGGCAAGGATTCGAAGACCGAACTCGTGCACTTCATCGGCAAAGACATCC
TGTATTTCCACGCCTTGTTCTGGCCGGCGATGCTCAAGTTCTCCGGCCACCGCCTGCCGACCGCCGTCTACGCGCACGGC
TTCCTGACCGTCAACGGCCAGAAGATGTCGAAGTCGCGCGGCACCTTCATCACCGCGTCGAGCTATCTCGACCAAGGCCT
CAACCCCGAGTGGCTGCGCTACTACTACGCTGCGAAGCTCAACGGCACGATGGAGGACATCGATCTCAACCTCGACGACT
TCGTCGCGCGCGTGAACTCGGACCTCGTCGGCAAGTTCATCAATATCGCGAGCCGCACGGCCGGCTTCATCCACAAGAAG
TTCGACGGCAAGCTCGCCGACGGCGTCGGCAACATTGAACTAATCGGCGAATTCCAGGCCGCGGCCGACACGATCGCCGC
GCACTACGAGGCGCGCGACTACGCCAAGGCGCTCAGGGAGATCATGGCGCTCGCCGACCGCGCGAACCAGTATGTTGCCG
ACGAAAAGCCGTGGGAACTGGCCAAGGACGACGCAGCGGTCTACCGCCTGCACGAGGTCTGCACGGTCGCGCTCAACCTG
TTCCGCCTGCTCACGCTTTACCTCAAGCCCGTGTTGCCCAAGCTCGCCGGCGAAGTCGAGCGCTTCCTCGACATCCCCGC
CCTCACGTGGAAAGACGCGCGAAGTCTTTTCATCCGCCAGGCCATCAACGCATACAGCCATCTCATGACCCGCATCGATC
CCAAATCCATCGAAGCCATGGTAGACGCCAACAAGCAGAACCTCGAACCCACTCCCGCCCCGGCCCCCGCCCGCCACGCC
GAGGCGCAGGCGCATGCCGCGCAGGCCGTCGAAGCACCGAAGACCGAGACGATCTCGATCGACGACTTCGCGAAGGTCGA
CCTGCGCATCGCGCGCATCGCCAACGCGGAGCACGTCGAAGGCGCCGACAAGCTGCTGCGCTTGACGCTCGACGTCGGTG
AACTCGGCCCCCGCCAGGTATTCGCCGGCATCAAGTCGGCCTACGCGCCCGAAGCGCTGGTCGGCCGGCTGACCGTCATG
GTCGCCAACCTCGCGCCGCGCAAGATGAAGTTCGGCATGAGCGAGGGCATGGTGCTCGCCGCCTCCAACCCCGACGGGCA
TAAAGACGACGTGCCCGGCCTCTTCATCCTCTCGCCCGACAGCGGCGCCGAGCCGGGCATGAAGGTCAAGTAA

Upstream 100 bases:

>100_bases
TCCTCAAGCAGCCGGGCTCGGGTTTTTAGGCGAAGCTCATCCAGCGCCGGCGGCCGCTCGCGCTAAAATTGCGGCTTCCT
GAGCGACCCGTCCGCTGTTC

Downstream 100 bases:

>100_bases
GCCGGTGAAGACGCTGCACCTGCAGATCGAGGGCCGCGTCCAGGGGGTATGGTTCCGCGAATCGATGCGGCGCGAGGCCG
AGCGCCTCGGCGTCGACGGC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MSRQILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMRGTNCRYMCADDTHGTAIMLRAEKEAITPEALIQRVWAEHTR
DFAGFHIGFDHYYSTHSDENREHASKIYLALKDAGLIEVKTIAQLYDPVKNLFLPDRFIKGECPKCGAADQYGDNCEVCG
ATYSPTELKNPVSAVSGATPVHKDSEHYFFKLGDCEAFLREWTTSGTLQAEAANKMQEWFAAGLNNWDISRDAPYFGFEI
PDAPGKYFYVWLDAPVGYMASFARYAKDHGLDFDAWWGKDSKTELVHFIGKDILYFHALFWPAMLKFSGHRLPTAVYAHG
FLTVNGQKMSKSRGTFITASSYLDQGLNPEWLRYYYAAKLNGTMEDIDLNLDDFVARVNSDLVGKFINIASRTAGFIHKK
FDGKLADGVGNIELIGEFQAAADTIAAHYEARDYAKALREIMALADRANQYVADEKPWELAKDDAAVYRLHEVCTVALNL
FRLLTLYLKPVLPKLAGEVERFLDIPALTWKDARSLFIRQAINAYSHLMTRIDPKSIEAMVDANKQNLEPTPAPAPARHA
EAQAHAAQAVEAPKTETISIDDFAKVDLRIARIANAEHVEGADKLLRLTLDVGELGPRQVFAGIKSAYAPEALVGRLTVM
VANLAPRKMKFGMSEGMVLAASNPDGHKDDVPGLFILSPDSGAEPGMKVK

Sequences:

>Translated_690_residues
MSRQILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMRGTNCRYMCADDTHGTAIMLRAEKEAITPEALIQRVWAEHTR
DFAGFHIGFDHYYSTHSDENREHASKIYLALKDAGLIEVKTIAQLYDPVKNLFLPDRFIKGECPKCGAADQYGDNCEVCG
ATYSPTELKNPVSAVSGATPVHKDSEHYFFKLGDCEAFLREWTTSGTLQAEAANKMQEWFAAGLNNWDISRDAPYFGFEI
PDAPGKYFYVWLDAPVGYMASFARYAKDHGLDFDAWWGKDSKTELVHFIGKDILYFHALFWPAMLKFSGHRLPTAVYAHG
FLTVNGQKMSKSRGTFITASSYLDQGLNPEWLRYYYAAKLNGTMEDIDLNLDDFVARVNSDLVGKFINIASRTAGFIHKK
FDGKLADGVGNIELIGEFQAAADTIAAHYEARDYAKALREIMALADRANQYVADEKPWELAKDDAAVYRLHEVCTVALNL
FRLLTLYLKPVLPKLAGEVERFLDIPALTWKDARSLFIRQAINAYSHLMTRIDPKSIEAMVDANKQNLEPTPAPAPARHA
EAQAHAAQAVEAPKTETISIDDFAKVDLRIARIANAEHVEGADKLLRLTLDVGELGPRQVFAGIKSAYAPEALVGRLTVM
VANLAPRKMKFGMSEGMVLAASNPDGHKDDVPGLFILSPDSGAEPGMKVK
>Mature_689_residues
SRQILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMRGTNCRYMCADDTHGTAIMLRAEKEAITPEALIQRVWAEHTRD
FAGFHIGFDHYYSTHSDENREHASKIYLALKDAGLIEVKTIAQLYDPVKNLFLPDRFIKGECPKCGAADQYGDNCEVCGA
TYSPTELKNPVSAVSGATPVHKDSEHYFFKLGDCEAFLREWTTSGTLQAEAANKMQEWFAAGLNNWDISRDAPYFGFEIP
DAPGKYFYVWLDAPVGYMASFARYAKDHGLDFDAWWGKDSKTELVHFIGKDILYFHALFWPAMLKFSGHRLPTAVYAHGF
LTVNGQKMSKSRGTFITASSYLDQGLNPEWLRYYYAAKLNGTMEDIDLNLDDFVARVNSDLVGKFINIASRTAGFIHKKF
DGKLADGVGNIELIGEFQAAADTIAAHYEARDYAKALREIMALADRANQYVADEKPWELAKDDAAVYRLHEVCTVALNLF
RLLTLYLKPVLPKLAGEVERFLDIPALTWKDARSLFIRQAINAYSHLMTRIDPKSIEAMVDANKQNLEPTPAPAPARHAE
AQAHAAQAVEAPKTETISIDDFAKVDLRIARIANAEHVEGADKLLRLTLDVGELGPRQVFAGIKSAYAPEALVGRLTVMV
ANLAPRKMKFGMSEGMVLAASNPDGHKDDVPGLFILSPDSGAEPGMKVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=647, Percent_Identity=27.3570324574961, Blast_Score=232, Evalue=7e-61,
Organism=Homo sapiens, GI24308436, Length=530, Percent_Identity=26.0377358490566, Blast_Score=145, Evalue=2e-34,
Organism=Homo sapiens, GI215490009, Length=108, Percent_Identity=34.2592592592593, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI45006986, Length=108, Percent_Identity=34.2592592592593, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI215490011, Length=108, Percent_Identity=34.2592592592593, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788432, Length=693, Percent_Identity=56.998556998557, Blast_Score=813, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=558, Percent_Identity=28.673835125448, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI212645096, Length=531, Percent_Identity=23.5404896421846, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71992781, Length=426, Percent_Identity=23.7089201877934, Blast_Score=109, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=566, Percent_Identity=31.8021201413428, Blast_Score=276, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6321610, Length=552, Percent_Identity=23.5507246376812, Blast_Score=113, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24655311, Length=514, Percent_Identity=30.1556420233463, Blast_Score=239, Evalue=5e-63,
Organism=Drosophila melanogaster, GI21358483, Length=525, Percent_Identity=25.1428571428571, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24651924, Length=105, Percent_Identity=36.1904761904762, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_THIDA (Q3SKG8)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314618.1
- ProteinModelPortal:   Q3SKG8
- STRING:   Q3SKG8
- GeneID:   3671624
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0860
- NMPDR:   fig|292415.3.peg.288
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   CEAVPES
- PhylomeDB:   Q3SKG8
- ProtClustDB:   PRK00133
- BioCyc:   TDEN292415:TBD_0860-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 76866; Mature: 76735

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 331-331

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMRGTNCRYMCADDTHGTAIMLRA
CCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHEEHHHCCCCCEEEECCCCCCCEEEEEC
EKEAITPEALIQRVWAEHTRDFAGFHIGFDHYYSTHSDENREHASKIYLALKDAGLIEVK
CCCCCCHHHHHHHHHHHHHHCCCCEEECHHHHCCCCCCCCHHHHHEEEEEEECCCCCHHH
TIAQLYDPVKNLFLPDRFIKGECPKCGAADQYGDNCEVCGATYSPTELKNPVSAVSGATP
HHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCCC
VHKDSEHYFFKLGDCEAFLREWTTSGTLQAEAANKMQEWFAAGLNNWDISRDAPYFGFEI
CCCCCCCEEEEECCHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEC
PDAPGKYFYVWLDAPVGYMASFARYAKDHGLDFDAWWGKDSKTELVHFIGKDILYFHALF
CCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
WPAMLKFSGHRLPTAVYAHGFLTVNGQKMSKSRGTFITASSYLDQGLNPEWLRYYYAAKL
HHHHHHHCCCCCCCEEEEEEEEEECCCHHCCCCCCEEEEHHHHHCCCCHHHHHHHHHHCC
NGTMEDIDLNLDDFVARVNSDLVGKFINIASRTAGFIHKKFDGKLADGVGNIELIGEFQA
CCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEECHHH
AADTIAAHYEARDYAKALREIMALADRANQYVADEKPWELAKDDAAVYRLHEVCTVALNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHH
FRLLTLYLKPVLPKLAGEVERFLDIPALTWKDARSLFIRQAINAYSHLMTRIDPKSIEAM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VDANKQNLEPTPAPAPARHAEAQAHAAQAVEAPKTETISIDDFAKVDLRIARIANAEHVE
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHEEEEHHCCCHHHC
GADKLLRLTLDVGELGPRQVFAGIKSAYAPEALVGRLTVMVANLAPRKMKFGMSEGMVLA
CHHHHEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEE
ASNPDGHKDDVPGLFILSPDSGAEPGMKVK
ECCCCCCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SRQILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMRGTNCRYMCADDTHGTAIMLRA
CCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHEEHHHCCCCCEEEECCCCCCCEEEEEC
EKEAITPEALIQRVWAEHTRDFAGFHIGFDHYYSTHSDENREHASKIYLALKDAGLIEVK
CCCCCCHHHHHHHHHHHHHHCCCCEEECHHHHCCCCCCCCHHHHHEEEEEEECCCCCHHH
TIAQLYDPVKNLFLPDRFIKGECPKCGAADQYGDNCEVCGATYSPTELKNPVSAVSGATP
HHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCCC
VHKDSEHYFFKLGDCEAFLREWTTSGTLQAEAANKMQEWFAAGLNNWDISRDAPYFGFEI
CCCCCCCEEEEECCHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEC
PDAPGKYFYVWLDAPVGYMASFARYAKDHGLDFDAWWGKDSKTELVHFIGKDILYFHALF
CCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
WPAMLKFSGHRLPTAVYAHGFLTVNGQKMSKSRGTFITASSYLDQGLNPEWLRYYYAAKL
HHHHHHHCCCCCCCEEEEEEEEEECCCHHCCCCCCEEEEHHHHHCCCCHHHHHHHHHHCC
NGTMEDIDLNLDDFVARVNSDLVGKFINIASRTAGFIHKKFDGKLADGVGNIELIGEFQA
CCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEECHHH
AADTIAAHYEARDYAKALREIMALADRANQYVADEKPWELAKDDAAVYRLHEVCTVALNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHH
FRLLTLYLKPVLPKLAGEVERFLDIPALTWKDARSLFIRQAINAYSHLMTRIDPKSIEAM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VDANKQNLEPTPAPAPARHAEAQAHAAQAVEAPKTETISIDDFAKVDLRIARIANAEHVE
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHEEEEHHCCCHHHC
GADKLLRLTLDVGELGPRQVFAGIKSAYAPEALVGRLTVMVANLAPRKMKFGMSEGMVLA
CHHHHEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEE
ASNPDGHKDDVPGLFILSPDSGAEPGMKVK
ECCCCCCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA