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The map label for this gene is cspA [H]

Identifier: 74316873

GI number: 74316873

Start: 904373

End: 904579

Strand: Reverse

Name: cspA [H]

Synonym: Tbd_0855

Alternate gene names: 74316873

Gene position: 904579-904373 (Counterclockwise)

Preceding gene: 74316877

Following gene: 74316871

Centisome position: 31.09

GC content: 55.56

Gene sequence:

>207_bases
ATGGCAACAGGTACAGTCAAATGGTTCAGCGACGCCAAAGGCTTTGGTTTCATCACGCCCGATGACGGCGGCGAGGATGT
CTTCGCGCATTTCTCGGCGATCCAGACCAACGGCTTCAAGACCTTGAAGGAAGGCCAGAAAGTCAGCTTCGAAGTCAGCC
AAGGCCCCAAAGGCAAGCAGGCTTCCAACATCCAGCCGCAAGGTTGA

Upstream 100 bases:

>100_bases
CAGACCGACGGCTAAAAAAATCAGGCCGCCTTGTCAATCCGCCAATGCATTCTGCTACCGCGGTTTTGTTTTAGTGTTAT
GCCCAGAGGAGGGAGTTTGA

Downstream 100 bases:

>100_bases
GTTGAGCAGGCAACAACAAAAACCCCGCCTCGGCGGGGTTTTCTTTTGATACGGCCCCGCCACAGCGGAGTTTTCTTTTG
CTACCGCCCCCGCCTCGACG

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 68; Mature: 67

Protein sequence:

>68_residues
MATGTVKWFSDAKGFGFITPDDGGEDVFAHFSAIQTNGFKTLKEGQKVSFEVSQGPKGKQASNIQPQG

Sequences:

>Translated_68_residues
MATGTVKWFSDAKGFGFITPDDGGEDVFAHFSAIQTNGFKTLKEGQKVSFEVSQGPKGKQASNIQPQG
>Mature_67_residues
ATGTVKWFSDAKGFGFITPDDGGEDVFAHFSAIQTNGFKTLKEGQKVSFEVSQGPKGKQASNIQPQG

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=66, Percent_Identity=65.1515151515152, Blast_Score=96, Evalue=4e-22,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=68.8524590163934, Blast_Score=96, Evalue=6e-22,
Organism=Escherichia coli, GI1789979, Length=62, Percent_Identity=66.1290322580645, Blast_Score=88, Evalue=9e-20,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=62.9032258064516, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1788126, Length=61, Percent_Identity=67.2131147540984, Blast_Score=87, Evalue=3e-19,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=62.9032258064516, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=59.6774193548387, Blast_Score=82, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24658520, Length=61, Percent_Identity=47.5409836065574, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7252; Mature: 7121

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGTVKWFSDAKGFGFITPDDGGEDVFAHFSAIQTNGFKTLKEGQKVSFEVSQGPKGKQ
CCCCCEEECCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCC
ASNIQPQG
CCCCCCCC
>Mature Secondary Structure 
ATGTVKWFSDAKGFGFITPDDGGEDVFAHFSAIQTNGFKTLKEGQKVSFEVSQGPKGKQ
CCCCEEECCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCC
ASNIQPQG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA