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The map label for this gene is rluE [H]

Identifier: 74316871

GI number: 74316871

Start: 902371

End: 902913

Strand: Reverse

Name: rluE [H]

Synonym: Tbd_0853

Alternate gene names: 74316871

Gene position: 902913-902371 (Counterclockwise)

Preceding gene: 74316873

Following gene: 74316870

Centisome position: 31.03

GC content: 66.85

Gene sequence:

>543_bases
GTGGCGCGATTGATTTTGTTCAACAAACCCTATGGCGTGCTGAGCCAGTTCACGCCCGAGGGGCGCTGGCAGGGTTTGCG
CGATCATCTCGCGCTGCCGGGCGTCTACGCGGCAGGACGTCTGGACGCCGACAGCGAAGGCCTGCTGATTTTAACCGATG
ACGGCCCGACCCAAGCCCGCATCGCCGACCCCCGGCACAAGTTGCCGAAGACCTACTGGGCGCAGGTGGAAGGCGCGCCG
GCCGACGCCGACCTCGAGCCGTTGCGCCGCGGTGTCGACCTCGGCGACTTCGTCACCCGTCCGGCAGGGGCGCGCATCAT
CGCCGAACCGCCCAGCCTCTGGCCGCGTAACCCGCCGATCCGCTTTCGCGCGAACATTCCGACGGCCTGGATCGAACTTG
TGATCGCCGAGGGCAAGAACCGCCAGGTGCGGCGCATGACGGCGAAGGTCGGCTTTCCGACGCTGCGCCTGATCCGCGCG
GCGGTCGGCGAATGGAATCTGAACGGGCTACAGCCCGGCCAATGGAAGGAACTGCATGTCTGA

Upstream 100 bases:

>100_bases
TGCTGATAAGTCATGGCGAGTAAATGCTGGGTGAATTAGAGGCGGGGCGGCGCGTGGCTAACGTTAAAATGGCGTCTTTT
CCGACGTTCGTTTTTCCAGC

Downstream 100 bases:

>100_bases
ACCGCTTCGATGGCTGCCCCACGTCGTCGTCGCCGCGATCGTCGAGCGCGACGGCCGCTACCTCTTCGTCGAGGAACACA
CGGCCGAGGGCCTGCGGCTC

Product: ribosomal large subunit pseudouridine synthase E

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MARLILFNKPYGVLSQFTPEGRWQGLRDHLALPGVYAAGRLDADSEGLLILTDDGPTQARIADPRHKLPKTYWAQVEGAP
ADADLEPLRRGVDLGDFVTRPAGARIIAEPPSLWPRNPPIRFRANIPTAWIELVIAEGKNRQVRRMTAKVGFPTLRLIRA
AVGEWNLNGLQPGQWKELHV

Sequences:

>Translated_180_residues
MARLILFNKPYGVLSQFTPEGRWQGLRDHLALPGVYAAGRLDADSEGLLILTDDGPTQARIADPRHKLPKTYWAQVEGAP
ADADLEPLRRGVDLGDFVTRPAGARIIAEPPSLWPRNPPIRFRANIPTAWIELVIAEGKNRQVRRMTAKVGFPTLRLIRA
AVGEWNLNGLQPGQWKELHV
>Mature_179_residues
ARLILFNKPYGVLSQFTPEGRWQGLRDHLALPGVYAAGRLDADSEGLLILTDDGPTQARIADPRHKLPKTYWAQVEGAPA
DADLEPLRRGVDLGDFVTRPAGARIIAEPPSLWPRNPPIRFRANIPTAWIELVIAEGKNRQVRRMTAKVGFPTLRLIRAA
VGEWNLNGLQPGQWKELHV

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=57.0621468926554, Blast_Score=206, Evalue=8e-55,
Organism=Escherichia coli, GI1788510, Length=181, Percent_Identity=34.2541436464088, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1787524, Length=185, Percent_Identity=33.5135135135135, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 20064; Mature: 19933

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLILFNKPYGVLSQFTPEGRWQGLRDHLALPGVYAAGRLDADSEGLLILTDDGPTQAR
CCEEEEEECCCHHHHHCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCEEEEECCCCCCEE
IADPRHKLPKTYWAQVEGAPADADLEPLRRGVDLGDFVTRPAGARIIAEPPSLWPRNPPI
ECCCHHHCCCCEEEEECCCCCCCCHHHHHCCCCCHHHHCCCCCCEEEECCCCCCCCCCCE
RFRANIPTAWIELVIAEGKNRQVRRMTAKVGFPTLRLIRAAVGEWNLNGLQPGQWKELHV
EEEECCCHHHEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEECCC
>Mature Secondary Structure 
ARLILFNKPYGVLSQFTPEGRWQGLRDHLALPGVYAAGRLDADSEGLLILTDDGPTQAR
CEEEEEECCCHHHHHCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCEEEEECCCCCCEE
IADPRHKLPKTYWAQVEGAPADADLEPLRRGVDLGDFVTRPAGARIIAEPPSLWPRNPPI
ECCCHHHCCCCEEEEECCCCCCCCHHHHHCCCCCHHHHCCCCCCEEEECCCCCCCCCCCE
RFRANIPTAWIELVIAEGKNRQVRRMTAKVGFPTLRLIRAAVGEWNLNGLQPGQWKELHV
EEEECCCHHHEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]