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The map label for this gene is cho [H]

Identifier: 74316845

GI number: 74316845

Start: 869458

End: 870870

Strand: Reverse

Name: cho [H]

Synonym: Tbd_0827

Alternate gene names: 74316845

Gene position: 870870-869458 (Counterclockwise)

Preceding gene: 74316846

Following gene: 74316844

Centisome position: 29.93

GC content: 68.29

Gene sequence:

>1413_bases
TTGCACACCACCGTGATTCCCCAGCGCCTCGCCTTCGTCGACCTCGAAACCACCGGCGCCAACGCCGCGCGTGACCGCGT
GACCGAAATCGGCGTCGTCGTCGTCGACGGCGACCGGGTCGAGACCTGGAGCACGCTCGTCAACCCCGAGCGCGCGATTC
CGCAATTCATCCAGCAGCTCACCGGCATCAGCAACGAGATGGTCGCCGACGCGCCGACCTTCGCACGCGTCGCCGACGAA
CTCGCCGAGCGGCTCGACGGGCGCCTCTTCATCGCGCATAACGCGCGCTTCGACTACGGCTTTCTCAAGCACGAATACCA
GCGCCTCGGCCGCCGCTTCCGCGCCGACGTGCTGTGCACGGTGCGCCTGTCGCGCCGGCTCTTTCCCGAACACTACCGCC
ACAACCTCGACAGCCTGATCGCGCGCCACGGCCTGGCAACCGAGGGCGACCGCCACCGTGCGCTCGCCGACGCCGACCTG
ATCTGGCAGTTTTGGCGCACGCTGCAGCGCGAACACGAGGCGCAGGCGCTCGCCGAGGCCGTCGCGCACCAACTCCTGCG
CCCCGCGCTGCCGCCGCACCTCGATCCCGCCCTGCTCGAAGACCTGCCCGAGTCGCCGGGGGTGTATCTCTTCTATGGCG
AAAACGACGTGCTGCTCTACGTCGGCAAGAGCGTCAATCTGCGCCAGCGCGTGTTCTCGCACTTCGCGGCCGACACCCGC
GAATACAAGGCGATGCAATTGACCCAGCAGGTGCGCCGCGTCGAGTGGCGCGAAACGGTCGGCGAACTCGGCGCGCTCTT
GCTCGAATCGCGGCTCATCAAGGAAGGCGCGCCCGCACACAACCGGCGGCTGCGCCGCGCGTCCGAACTCTGCGCCTGGC
GGCTCGACGAAGTCGCGCCCGGCGACTTCCGCCCGGTGCTCGTGACCGCCGACGACCCGGACTTCGGCCGCGACGGCGAC
CTCTTCGGCCCGTTCTCGAGCCGCCGCCAGGCGACGCTCGCCTTGCGCAAGATCGCCGACGCGCATCAACTCTGCCCGGT
CGTCCTCGGGCTCGAGAAACCCGCGCGCCCCGACAGCCCCTGCTTCGCCTTTCAGGTACAAAAATGCCGCGGCGCCTGCG
CGGGCAAGGAGACGGCCGGCCTTCACAGCGCCCGGCTGATGGCCGCGCTCGCGAAAACGAAAATGAAAAGCTGGGCCTAC
CCCGGCGCGATCGGACTCGTCGAGCGCGACGACTTTCTCGACGTCGAGGAAGTCCACGTCGTGCACGCCTGGCGCTATCT
GGGCACCGCGAAGAGCGAAGCCGAGATCGGCGAACTGCTCGAACACGCGGCACGCGCGCCCTTCGATCTCGACACCTACA
AGCTGCTCAAGACGCAACTCGCCAAGTCGAAGTTCACGATCCGCGCCCTGTAG

Upstream 100 bases:

>100_bases
CCCGCTGCTCGGCCGCCTTGTGACGCATTCGCGCGACTTTCACTGACGCTTCATGCACGCGGAACTCGCGACGCGCTTCG
CCCTCTGACACGCCATTCCT

Downstream 100 bases:

>100_bases
TCCGCGTCCGCTTCTGATGGTCGGGGCGGAACCCCAAGTCCCCACGATCGTCTATCAATAAAGCTCGAAGAAAAAGCCGG
ACACGGGACCGCTGTCCGCA

Product: DNA polymerase III subunit epsilon

Products: diphosphate; DNAn+1

Alternate protein names: Endonuclease cho; UvrC homolog protein [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MHTTVIPQRLAFVDLETTGANAARDRVTEIGVVVVDGDRVETWSTLVNPERAIPQFIQQLTGISNEMVADAPTFARVADE
LAERLDGRLFIAHNARFDYGFLKHEYQRLGRRFRADVLCTVRLSRRLFPEHYRHNLDSLIARHGLATEGDRHRALADADL
IWQFWRTLQREHEAQALAEAVAHQLLRPALPPHLDPALLEDLPESPGVYLFYGENDVLLYVGKSVNLRQRVFSHFAADTR
EYKAMQLTQQVRRVEWRETVGELGALLLESRLIKEGAPAHNRRLRRASELCAWRLDEVAPGDFRPVLVTADDPDFGRDGD
LFGPFSSRRQATLALRKIADAHQLCPVVLGLEKPARPDSPCFAFQVQKCRGACAGKETAGLHSARLMAALAKTKMKSWAY
PGAIGLVERDDFLDVEEVHVVHAWRYLGTAKSEAEIGELLEHAARAPFDLDTYKLLKTQLAKSKFTIRAL

Sequences:

>Translated_470_residues
MHTTVIPQRLAFVDLETTGANAARDRVTEIGVVVVDGDRVETWSTLVNPERAIPQFIQQLTGISNEMVADAPTFARVADE
LAERLDGRLFIAHNARFDYGFLKHEYQRLGRRFRADVLCTVRLSRRLFPEHYRHNLDSLIARHGLATEGDRHRALADADL
IWQFWRTLQREHEAQALAEAVAHQLLRPALPPHLDPALLEDLPESPGVYLFYGENDVLLYVGKSVNLRQRVFSHFAADTR
EYKAMQLTQQVRRVEWRETVGELGALLLESRLIKEGAPAHNRRLRRASELCAWRLDEVAPGDFRPVLVTADDPDFGRDGD
LFGPFSSRRQATLALRKIADAHQLCPVVLGLEKPARPDSPCFAFQVQKCRGACAGKETAGLHSARLMAALAKTKMKSWAY
PGAIGLVERDDFLDVEEVHVVHAWRYLGTAKSEAEIGELLEHAARAPFDLDTYKLLKTQLAKSKFTIRAL
>Mature_470_residues
MHTTVIPQRLAFVDLETTGANAARDRVTEIGVVVVDGDRVETWSTLVNPERAIPQFIQQLTGISNEMVADAPTFARVADE
LAERLDGRLFIAHNARFDYGFLKHEYQRLGRRFRADVLCTVRLSRRLFPEHYRHNLDSLIARHGLATEGDRHRALADADL
IWQFWRTLQREHEAQALAEAVAHQLLRPALPPHLDPALLEDLPESPGVYLFYGENDVLLYVGKSVNLRQRVFSHFAADTR
EYKAMQLTQQVRRVEWRETVGELGALLLESRLIKEGAPAHNRRLRRASELCAWRLDEVAPGDFRPVLVTADDPDFGRDGD
LFGPFSSRRQATLALRKIADAHQLCPVVLGLEKPARPDSPCFAFQVQKCRGACAGKETAGLHSARLMAALAKTKMKSWAY
PGAIGLVERDDFLDVEEVHVVHAWRYLGTAKSEAEIGELLEHAARAPFDLDTYKLLKTQLAKSKFTIRAL

Specific function: Incises the DNA at the 3' side of a lesion during nucleotide excision repair. Incises the DNA farther away from the lesion than uvrC. Not able to incise the 5' site of a lesion. When a lesion remains because uvrC is not able to induce the 3' incision, cho

COG id: COG0322

COG function: function code L; Nuclease subunit of the excinuclease complex

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrC C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788037, Length=281, Percent_Identity=41.9928825622776, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1786409, Length=189, Percent_Identity=30.1587301587302, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081999, Length=180, Percent_Identity=29.4444444444444, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000305 [H]

Pfam domain/function: PF01541 GIY-YIG [H]

EC number: 2.7.7.7

Molecular weight: Translated: 53027; Mature: 53027

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS50164 UVRC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTTVIPQRLAFVDLETTGANAARDRVTEIGVVVVDGDRVETWSTLVNPERAIPQFIQQL
CCCCCCCCHHEEEEEECCCCCHHHHHHHHEEEEEECCCCCHHHHHHCCHHHHHHHHHHHH
TGISNEMVADAPTFARVADELAERLDGRLFIAHNARFDYGFLKHEYQRLGRRFRADVLCT
HCCCCHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
VRLSRRLFPEHYRHNLDSLIARHGLATEGDRHRALADADLIWQFWRTLQREHEAQALAEA
HHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAHQLLRPALPPHLDPALLEDLPESPGVYLFYGENDVLLYVGKSVNLRQRVFSHFAADTR
HHHHHHHCCCCCCCCHHHHHHCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHH
EYKAMQLTQQVRRVEWRETVGELGALLLESRLIKEGAPAHNRRLRRASELCAWRLDEVAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
GDFRPVLVTADDPDFGRDGDLFGPFSSRRQATLALRKIADAHQLCPVVLGLEKPARPDSP
CCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
CFAFQVQKCRGACAGKETAGLHSARLMAALAKTKMKSWAYPGAIGLVERDDFLDVEEVHV
HHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHH
VHAWRYLGTAKSEAEIGELLEHAARAPFDLDTYKLLKTQLAKSKFTIRAL
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEC
>Mature Secondary Structure
MHTTVIPQRLAFVDLETTGANAARDRVTEIGVVVVDGDRVETWSTLVNPERAIPQFIQQL
CCCCCCCCHHEEEEEECCCCCHHHHHHHHEEEEEECCCCCHHHHHHCCHHHHHHHHHHHH
TGISNEMVADAPTFARVADELAERLDGRLFIAHNARFDYGFLKHEYQRLGRRFRADVLCT
HCCCCHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
VRLSRRLFPEHYRHNLDSLIARHGLATEGDRHRALADADLIWQFWRTLQREHEAQALAEA
HHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAHQLLRPALPPHLDPALLEDLPESPGVYLFYGENDVLLYVGKSVNLRQRVFSHFAADTR
HHHHHHHCCCCCCCCHHHHHHCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHH
EYKAMQLTQQVRRVEWRETVGELGALLLESRLIKEGAPAHNRRLRRASELCAWRLDEVAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
GDFRPVLVTADDPDFGRDGDLFGPFSSRRQATLALRKIADAHQLCPVVLGLEKPARPDSP
CCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
CFAFQVQKCRGACAGKETAGLHSARLMAALAKTKMKSWAYPGAIGLVERDDFLDVEEVHV
HHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHH
VHAWRYLGTAKSEAEIGELLEHAARAPFDLDTYKLLKTQLAKSKFTIRAL
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]