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The map label for this gene is clpB [H]

Identifier: 74316833

GI number: 74316833

Start: 855994

End: 858573

Strand: Direct

Name: clpB [H]

Synonym: Tbd_0815

Alternate gene names: 74316833

Gene position: 855994-858573 (Clockwise)

Preceding gene: 74316829

Following gene: 74316834

Centisome position: 29.42

GC content: 66.16

Gene sequence:

>2580_bases
ATGCGTTTTGACAAGCTCACCACCCAGTTCCAGCAGGCGCTTTCCGACGCGCAAAGCCTGGCCGTCGGCGGCGACCACGC
CTACATCGAGCCGCAGCACCTGCTGCTCGCCCTGCTCAACCAGGAGGGCGGCGGCGCGGGGTCGATCCTCGGCCGCGCCG
GCGTCCAGGTCCCGGCGCTCAAGGCCGCGTTGACCCAGGCATTGACGCGCCTGCCCAAGGTGCAGGGGCAGGGCGGCGAG
GTCAGCATCTCGCGCGACCTCAACAACCTGCTCAACCTGACCGACAAGGAGGCGATGAAGCGCAACGACGCCTACATCGC
ATCGGAGCTTTTCCTGCTCGCCGCGCTCGACGACAAAGGCGAGACCGGGCGCCTGCTGAAACAGCACGGCGCGCAGAAGA
CCGCGCTGGAACAGGCCATCCAGGCCGTGCGCGGCGGCGAGAACGTGCAGTCGCAGGAAGCCGAAGGCCAGCGCGAAGCG
CTCGCCAAATACACCCTCGACCTCACGCAGCGCGCGCGTGACGGCAAGCTCGACCCGGTCATCGGGCGCGACGACGAGAT
TCGCCGCTCGATCCAGATCCTGCAGCGCCGCACCAAGAACAACCCCGTGCTGATCGGCGAACCCGGCGTCGGCAAGACCG
CGATCGTCGAAGGTCTCGCGCAGCGCATCGTCAACGACGAGGTGCCGGAAACGCTCAAGGGCAAGAAGGTGCTGGTGCTC
GACCTCGCCGCGCTGCTCGCCGGCGCGAAATACCGCGGCGAATTCGAGGAGCGCCTGAAGGCCGTGCTCAAGGAGCTCGC
GATGGACCCCGGCCGCTACATCGTCTTCCTCGACGAAATCCACACGCTCGTCGGGGCCGGCAAGGCCGAAGGCGCGATCG
ACGCCGGCAACATGCTGAAGCCCGCGCTCGCGCGGGGCGAACTGCACCTGATCGGCGCGACCACGCTCGACGAATACCGC
AAGTACATCGAGAAGGACGCCGCGCTCGAGCGCCGCTTCCAGAAGGTCATGGTCGACGAGCCCAGCGTCGAATCGACGAT
CGCGATCCTGCGCGGCCTGCAGGAGCGCTACGAACTGCACCACGGCGTCGACATCACCGACCCGGCGATCGTCGCCGCGG
CCGAACTCTCGCATCGCTACATCACCGACCGTTTCCTGCCCGACAAGGCGATCGACCTGATCGACGAGGCCGCCGCGCGG
ATCAAGATGGAAATCGACTCCAAGCCCGAGGTCATGGACAAACTCGACCGCCGCATCATCCAGCTCAAGATCGAGCGCGA
GGCGGTCAAGAAGGAGACCGACGAGGCCTCGAAAAAGCGGCTCGGCCTGCTCGAAGACGAGATCGACAAGCTCGGCCGCG
AATACGCCGACCTCGAAGAGGTGTGGAAGTCGGAGAAGGCCCAGGTCCAGGGCTCCGCGCATCTCAAGGAAGAGATCGAC
AAGCTGCGCGGCGAGATGGCCGACCTGCAGCGCCAGGGCAAGCTCGACAAGGTCGCCGAGATCCAGTACGGCAAGCTGCC
GCAGCTCGAGGCGCAGCTCAAGCACGCCGAAGCCAGCGGCGAGAAGCCTGTGTTCAAGCTCCTGCGCACCGAGGTCGGCG
CCGAAGAGATCGCCGAGGTCGTCTCCCGCGCGACCGGCATCCCGGTCTCGAAGATGATGCAGGGCGAGCGCGACAAGCTC
ATGCACATGGAAGACCACCTGCACGGCCGCGTCGTCGGCCAGGACGAAGCCGTGGCCGTCGTCTCCGACGCGATCCGCCG
TTCACGCGCGGGGCTTTCCGACCCCAACCGGCCGCTCGGCTCCTTTCTGTTCCTGGGCCCCACGGGCGTCGGCAAGACCG
AGTTGTGCAAGACGCTCGCCGAATACCTGTTCGATTCCGCCGAGCACATGGTGCGCATCGACATGAGCGAATTCATGGAG
AAGCACTCGGTCGCGCGCCTGATCGGCGCGCCGCCCGGCTACGTCGGCTACGAAGAGGGCGGCTACCTGACCGAAGCCGT
GCGCCGCAAGCCGTACAGCGTGATCCTCATGGACGAAATCGAGAAGGCCCACCCCGACGTCTTCAACGTGCTCCTGCAGG
TGCTCGACGACGGCCGGCTCACCGACGGCCAGGGCCGCACCGTCGACTTCCGCAACACCGTGATCGTCATGACGTCCAAC
CTCGGCAGCCAGATGATCCAGCAGATGTCGGGCGACGACTACCAGGTCGTCAAGCTCGCCGTACTCGGCGAAGTGAAGTC
CTACTTCCGCCCCGAGTTCATCAACCGCATCGATGAGGTCGTCGTCTTCCACGCGCTCTCGGAGTCGAACATCGCGAGCA
TCGCGCGCATCCAGCTCAAGGCGCTCGAAGCGCGCCTCGCCGCGATGGAGATGAAGCTCGACGTCACCGACGCCGCGCTC
GCCGAAATCGCCAAGGCCGGCTTCGACCCCGTCTACGGCGCGCGGCCGCTCAAGCGTGCGATCCAGAGCGAGCTCGAAAA
CGCGCTGGCTAAGGAAATCCTGTCCGGACGCTTCGCGGCCAAGGACACGATCCGGGTCGACGCGGTGGATGGGAAGTTCA
GCTTTGAGAAGGCGGCGTAA

Upstream 100 bases:

>100_bases
TACGGAGCGGCCGGACTTCGTGCCCCGCGCCTTGCCCTGCATCCTGCAGGAAACCGGCCGGGTCACGATTCCAACTTGAA
CCGACTCTTGCGAGGCCACT

Downstream 100 bases:

>100_bases
GCGGCTGGAATTTCGCTAAAGGCGAAGGAAAAAGGGGGGCGGGTTTCGCCCCTTTCTTTAGGCGCTCGAAAAAATCGGTC
AGCCGTGAAGAAGCGAAAGG

Product: ClpB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 859; Mature: 859

Protein sequence:

>859_residues
MRFDKLTTQFQQALSDAQSLAVGGDHAYIEPQHLLLALLNQEGGGAGSILGRAGVQVPALKAALTQALTRLPKVQGQGGE
VSISRDLNNLLNLTDKEAMKRNDAYIASELFLLAALDDKGETGRLLKQHGAQKTALEQAIQAVRGGENVQSQEAEGQREA
LAKYTLDLTQRARDGKLDPVIGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNDEVPETLKGKKVLVL
DLAALLAGAKYRGEFEERLKAVLKELAMDPGRYIVFLDEIHTLVGAGKAEGAIDAGNMLKPALARGELHLIGATTLDEYR
KYIEKDAALERRFQKVMVDEPSVESTIAILRGLQERYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAAR
IKMEIDSKPEVMDKLDRRIIQLKIEREAVKKETDEASKKRLGLLEDEIDKLGREYADLEEVWKSEKAQVQGSAHLKEEID
KLRGEMADLQRQGKLDKVAEIQYGKLPQLEAQLKHAEASGEKPVFKLLRTEVGAEEIAEVVSRATGIPVSKMMQGERDKL
MHMEDHLHGRVVGQDEAVAVVSDAIRRSRAGLSDPNRPLGSFLFLGPTGVGKTELCKTLAEYLFDSAEHMVRIDMSEFME
KHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILMDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIVMTSN
LGSQMIQQMSGDDYQVVKLAVLGEVKSYFRPEFINRIDEVVVFHALSESNIASIARIQLKALEARLAAMEMKLDVTDAAL
AEIAKAGFDPVYGARPLKRAIQSELENALAKEILSGRFAAKDTIRVDAVDGKFSFEKAA

Sequences:

>Translated_859_residues
MRFDKLTTQFQQALSDAQSLAVGGDHAYIEPQHLLLALLNQEGGGAGSILGRAGVQVPALKAALTQALTRLPKVQGQGGE
VSISRDLNNLLNLTDKEAMKRNDAYIASELFLLAALDDKGETGRLLKQHGAQKTALEQAIQAVRGGENVQSQEAEGQREA
LAKYTLDLTQRARDGKLDPVIGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNDEVPETLKGKKVLVL
DLAALLAGAKYRGEFEERLKAVLKELAMDPGRYIVFLDEIHTLVGAGKAEGAIDAGNMLKPALARGELHLIGATTLDEYR
KYIEKDAALERRFQKVMVDEPSVESTIAILRGLQERYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAAR
IKMEIDSKPEVMDKLDRRIIQLKIEREAVKKETDEASKKRLGLLEDEIDKLGREYADLEEVWKSEKAQVQGSAHLKEEID
KLRGEMADLQRQGKLDKVAEIQYGKLPQLEAQLKHAEASGEKPVFKLLRTEVGAEEIAEVVSRATGIPVSKMMQGERDKL
MHMEDHLHGRVVGQDEAVAVVSDAIRRSRAGLSDPNRPLGSFLFLGPTGVGKTELCKTLAEYLFDSAEHMVRIDMSEFME
KHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILMDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIVMTSN
LGSQMIQQMSGDDYQVVKLAVLGEVKSYFRPEFINRIDEVVVFHALSESNIASIARIQLKALEARLAAMEMKLDVTDAAL
AEIAKAGFDPVYGARPLKRAIQSELENALAKEILSGRFAAKDTIRVDAVDGKFSFEKAA
>Mature_859_residues
MRFDKLTTQFQQALSDAQSLAVGGDHAYIEPQHLLLALLNQEGGGAGSILGRAGVQVPALKAALTQALTRLPKVQGQGGE
VSISRDLNNLLNLTDKEAMKRNDAYIASELFLLAALDDKGETGRLLKQHGAQKTALEQAIQAVRGGENVQSQEAEGQREA
LAKYTLDLTQRARDGKLDPVIGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNDEVPETLKGKKVLVL
DLAALLAGAKYRGEFEERLKAVLKELAMDPGRYIVFLDEIHTLVGAGKAEGAIDAGNMLKPALARGELHLIGATTLDEYR
KYIEKDAALERRFQKVMVDEPSVESTIAILRGLQERYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAAR
IKMEIDSKPEVMDKLDRRIIQLKIEREAVKKETDEASKKRLGLLEDEIDKLGREYADLEEVWKSEKAQVQGSAHLKEEID
KLRGEMADLQRQGKLDKVAEIQYGKLPQLEAQLKHAEASGEKPVFKLLRTEVGAEEIAEVVSRATGIPVSKMMQGERDKL
MHMEDHLHGRVVGQDEAVAVVSDAIRRSRAGLSDPNRPLGSFLFLGPTGVGKTELCKTLAEYLFDSAEHMVRIDMSEFME
KHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILMDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIVMTSN
LGSQMIQQMSGDDYQVVKLAVLGEVKSYFRPEFINRIDEVVVFHALSESNIASIARIQLKALEARLAAMEMKLDVTDAAL
AEIAKAGFDPVYGARPLKRAIQSELENALAKEILSGRFAAKDTIRVDAVDGKFSFEKAA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=318, Percent_Identity=36.1635220125786, Blast_Score=206, Evalue=8e-53,
Organism=Escherichia coli, GI1788943, Length=848, Percent_Identity=66.8632075471698, Blast_Score=1148, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=460, Percent_Identity=39.7826086956522, Blast_Score=284, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=704, Percent_Identity=53.125, Blast_Score=750, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=865, Percent_Identity=42.6589595375723, Blast_Score=674, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 94796; Mature: 94796

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDKLTTQFQQALSDAQSLAVGGDHAYIEPQHLLLALLNQEGGGAGSILGRAGVQVPAL
CCCHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHCCCCCCHHHHHHCCCCCHHH
KAALTQALTRLPKVQGQGGEVSISRDLNNLLNLTDKEAMKRNDAYIASELFLLAALDDKG
HHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHCCHHHHHHHHHEEEECCCC
ETGRLLKQHGAQKTALEQAIQAVRGGENVQSQEAEGQREALAKYTLDLTQRARDGKLDPV
CCHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHHHHCCCCCCCC
IGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNDEVPETLKGKKVLVL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHCCCEEHHH
DLAALLAGAKYRGEFEERLKAVLKELAMDPGRYIVFLDEIHTLVGAGKAEGAIDAGNMLK
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHLIGATTLDEYRKYIEKDAALERRFQKVMVDEPSVESTIAILRGLQERYELH
HHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAARIKMEIDSKPEVMDKLDRRII
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHH
QLKIEREAVKKETDEASKKRLGLLEDEIDKLGREYADLEEVWKSEKAQVQGSAHLKEEID
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KLRGEMADLQRQGKLDKVAEIQYGKLPQLEAQLKHAEASGEKPVFKLLRTEVGAEEIAEV
HHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHH
VSRATGIPVSKMMQGERDKLMHMEDHLHGRVVGQDEAVAVVSDAIRRSRAGLSDPNRPLG
HHHHCCCCHHHHHCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
SFLFLGPTGVGKTELCKTLAEYLFDSAEHMVRIDMSEFMEKHSVARLIGAPPGYVGYEEG
CEEEECCCCCCHHHHHHHHHHHHHCCHHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCC
GYLTEAVRRKPYSVILMDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIVMTSN
CHHHHHHHCCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECC
LGSQMIQQMSGDDYQVVKLAVLGEVKSYFRPEFINRIDEVVVFHALSESNIASIARIQLK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALEARLAAMEMKLDVTDAALAEIAKAGFDPVYGARPLKRAIQSELENALAKEILSGRFAA
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
KDTIRVDAVDGKFSFEKAA
CCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MRFDKLTTQFQQALSDAQSLAVGGDHAYIEPQHLLLALLNQEGGGAGSILGRAGVQVPAL
CCCHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHCCCCCCHHHHHHCCCCCHHH
KAALTQALTRLPKVQGQGGEVSISRDLNNLLNLTDKEAMKRNDAYIASELFLLAALDDKG
HHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHCCHHHHHHHHHEEEECCCC
ETGRLLKQHGAQKTALEQAIQAVRGGENVQSQEAEGQREALAKYTLDLTQRARDGKLDPV
CCHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHHHHCCCCCCCC
IGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNDEVPETLKGKKVLVL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHCCCEEHHH
DLAALLAGAKYRGEFEERLKAVLKELAMDPGRYIVFLDEIHTLVGAGKAEGAIDAGNMLK
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHLIGATTLDEYRKYIEKDAALERRFQKVMVDEPSVESTIAILRGLQERYELH
HHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAARIKMEIDSKPEVMDKLDRRII
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHH
QLKIEREAVKKETDEASKKRLGLLEDEIDKLGREYADLEEVWKSEKAQVQGSAHLKEEID
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KLRGEMADLQRQGKLDKVAEIQYGKLPQLEAQLKHAEASGEKPVFKLLRTEVGAEEIAEV
HHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHH
VSRATGIPVSKMMQGERDKLMHMEDHLHGRVVGQDEAVAVVSDAIRRSRAGLSDPNRPLG
HHHHCCCCHHHHHCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
SFLFLGPTGVGKTELCKTLAEYLFDSAEHMVRIDMSEFMEKHSVARLIGAPPGYVGYEEG
CEEEECCCCCCHHHHHHHHHHHHHCCHHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCC
GYLTEAVRRKPYSVILMDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIVMTSN
CHHHHHHHCCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECC
LGSQMIQQMSGDDYQVVKLAVLGEVKSYFRPEFINRIDEVVVFHALSESNIASIARIQLK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALEARLAAMEMKLDVTDAALAEIAKAGFDPVYGARPLKRAIQSELENALAKEILSGRFAA
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
KDTIRVDAVDGKFSFEKAA
CCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]