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The map label for this gene is ligA [H]

Identifier: 74316830

GI number: 74316830

Start: 853494

End: 854414

Strand: Reverse

Name: ligA [H]

Synonym: Tbd_0812

Alternate gene names: 74316830

Gene position: 854414-853494 (Counterclockwise)

Preceding gene: 74316831

Following gene: 74316827

Centisome position: 29.36

GC content: 70.03

Gene sequence:

>921_bases
ATGGCTCGACGACTGAGCCGGCCCGCTACCGTTCGGCCAGAAACCGTTCGACCTGCGCGCGTCGAAGGCCTGAAGCGCGT
AGCCGCCGCGCTGGCACTCGCCTCGGCGCTGTGCTGGCCGCACGCGGCCCGCACCGAGGCGCCGCCCGCGGCACCCGCGA
TGCTGCTCGCGAAGGTCTACGCCGCCGACGTCGACGTCACGCAGTATTGGGTCAGCGAGAAATTCGACGGCGTGCGCGCG
CAGTGGGACGGCCACGTCCTGCGCTTTCGCGGCGGCGGCCTCGCTCCGGCGCCGGCATGGTTCACGGCGGATTTTCCGCG
CGTGCCGCTCGATGGCGAGCTCTGGATCGCCCGCGATGCGTTCGACGCGTTGTCCGGAACGGTGCGCAAAGCCCCGCCGG
TCGACGCCGAATGGCGACGCGTGCGTTACCTGGTATTCGAACTGCCGGGCGCGCCGGGTGACTTCAGCGCGCGCGTCGGG
CAGATGCGTGAGGTCATCGAACAGGCGGCTGTCCCCTGGCTCGAGGCCGTCGCGCAGACGCGCGTCGCGAGCCGCGAAGA
CTTGATGCAAAGGCTAGACGCCGAGGTGCGCGCTGGCGGCGAAGGCCTGATGCTGCACCGCGCCGATGCGCCCTATCTCA
CCGGGCGCAGCGATGCGCTGCTGAAGCTCAAGCCCTGGCTCGACGCCGAGGCCATCGTCGTCGGCTACGTGCCGGGCAAG
GGAAAGTACGCAGGCATGACCGGCGCGCTGCGGATGGAAACGCCGGACGGCAAGCGCTTTCGCCTCGGCAGCGGACTCAG
TGACGCGCTGCGCCGCCAGCCGCCGCCCGTCGGCACGCGTATCACCTATCGCTATACGGCGCTGACGAAGAACGGCATGC
CGCGGTTTCCGCGTTACCTGCGGGTGCGCGAGGATTTTTAG

Upstream 100 bases:

>100_bases
TGCTGTCGGGCAACGCGCCGGCACGCGCGCTCTACGAAAAGCTCGGGTTCGACGACTACCGGCTCGACCCGGCGCTGGGC
AAGGCGCGGTTCATGCAGAA

Downstream 100 bases:

>100_bases
ACAGGCGTCGAGGCGAGGGGCCCCGCCACGCAGCGAATGGAATCCCTCAGACGCCTCGCATGTCGCTGAAGCTGGGCGCG
ACGCGCAGGCGGCGCCGCGC

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MARRLSRPATVRPETVRPARVEGLKRVAAALALASALCWPHAARTEAPPAAPAMLLAKVYAADVDVTQYWVSEKFDGVRA
QWDGHVLRFRGGGLAPAPAWFTADFPRVPLDGELWIARDAFDALSGTVRKAPPVDAEWRRVRYLVFELPGAPGDFSARVG
QMREVIEQAAVPWLEAVAQTRVASREDLMQRLDAEVRAGGEGLMLHRADAPYLTGRSDALLKLKPWLDAEAIVVGYVPGK
GKYAGMTGALRMETPDGKRFRLGSGLSDALRRQPPPVGTRITYRYTALTKNGMPRFPRYLRVREDF

Sequences:

>Translated_306_residues
MARRLSRPATVRPETVRPARVEGLKRVAAALALASALCWPHAARTEAPPAAPAMLLAKVYAADVDVTQYWVSEKFDGVRA
QWDGHVLRFRGGGLAPAPAWFTADFPRVPLDGELWIARDAFDALSGTVRKAPPVDAEWRRVRYLVFELPGAPGDFSARVG
QMREVIEQAAVPWLEAVAQTRVASREDLMQRLDAEVRAGGEGLMLHRADAPYLTGRSDALLKLKPWLDAEAIVVGYVPGK
GKYAGMTGALRMETPDGKRFRLGSGLSDALRRQPPPVGTRITYRYTALTKNGMPRFPRYLRVREDF
>Mature_305_residues
ARRLSRPATVRPETVRPARVEGLKRVAAALALASALCWPHAARTEAPPAAPAMLLAKVYAADVDVTQYWVSEKFDGVRAQ
WDGHVLRFRGGGLAPAPAWFTADFPRVPLDGELWIARDAFDALSGTVRKAPPVDAEWRRVRYLVFELPGAPGDFSARVGQ
MREVIEQAAVPWLEAVAQTRVASREDLMQRLDAEVRAGGEGLMLHRADAPYLTGRSDALLKLKPWLDAEAIVVGYVPGKG
KYAGMTGALRMETPDGKRFRLGSGLSDALRRQPPPVGTRITYRYTALTKNGMPRFPRYLRVREDF

Specific function: Catalyzes efficient strand joining on a single nicked DNA [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 33813; Mature: 33682

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRLSRPATVRPETVRPARVEGLKRVAAALALASALCWPHAARTEAPPAAPAMLLAKVY
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
AADVDVTQYWVSEKFDGVRAQWDGHVLRFRGGGLAPAPAWFTADFPRVPLDGELWIARDA
HCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCEEECCCCCCCCCCCEEEEHHH
FDALSGTVRKAPPVDAEWRRVRYLVFELPGAPGDFSARVGQMREVIEQAAVPWLEAVAQT
HHHHCCCCCCCCCCCHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
RVASREDLMQRLDAEVRAGGEGLMLHRADAPYLTGRSDALLKLKPWLDAEAIVVGYVPGK
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCCEEEECCCCCCCEEEEEEECCC
GKYAGMTGALRMETPDGKRFRLGSGLSDALRRQPPPVGTRITYRYTALTKNGMPRFPRYL
CCCCCCCCEEEEECCCCCEEEECCCHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHH
RVREDF
EEECCC
>Mature Secondary Structure 
ARRLSRPATVRPETVRPARVEGLKRVAAALALASALCWPHAARTEAPPAAPAMLLAKVY
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
AADVDVTQYWVSEKFDGVRAQWDGHVLRFRGGGLAPAPAWFTADFPRVPLDGELWIARDA
HCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCEEECCCCCCCCCCCEEEEHHH
FDALSGTVRKAPPVDAEWRRVRYLVFELPGAPGDFSARVGQMREVIEQAAVPWLEAVAQT
HHHHCCCCCCCCCCCHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
RVASREDLMQRLDAEVRAGGEGLMLHRADAPYLTGRSDALLKLKPWLDAEAIVVGYVPGK
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCCEEEECCCCCCCEEEEEEECCC
GKYAGMTGALRMETPDGKRFRLGSGLSDALRRQPPPVGTRITYRYTALTKNGMPRFPRYL
CCCCCCCCEEEEECCCCCEEEECCCHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHH
RVREDF
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550458; 7542800; 9060431 [H]