Click here to switch to the map view.

The map label for this gene is tsf

Identifier: 74316804

GI number: 74316804

Start: 829439

End: 830311

Strand: Direct

Name: tsf

Synonym: Tbd_0786

Alternate gene names: 74316804

Gene position: 829439-830311 (Clockwise)

Preceding gene: 74316803

Following gene: 74316805

Centisome position: 28.5

GC content: 63.69

Gene sequence:

>873_bases
ATGGCTGAAATCACTGCAAGCATGGTCAAGGATCTGCGGGAAAAAACCGACGCGCCGATGATGGACTGCAAGAAGGCGCT
GACCGAGGCCGGCGGCGACATGCAGAAGGCCGAGGAAATCCTGCGCGTGCGTTTCGGCAACAAGGCCGCCAAGAGCGCCG
GCCGCGTCGCCGCCGAAGGCGTGGTGACGATCGCGATCAGCCCCGACGGCAAATCCGCCGCGATGGTCGAAGTCAACTGC
GAGACCGACTTCGTGGCGAAGAACGACGACTTTCTTGCGCTGTCGGCCGCGCTGGCCGAGATGGTGCTGACCCAGAAGCC
GGCCGACGTCGCCGCCCTGTCGGCCCTGCCGTACAAGAATTCGACGGTCGAGGGGTTCCGCACCGAACTCGTCGGCAAGA
TCGGTGAAAACATGTCGGTGCGCCGCTTCGTGCGTCTCGACGGCCAGGGCAAATTCGCCAGCTACATCCACGGCGGCAAG
ATCGGCGTGCTGGTCGACGTGACCGGCGACGAGGCGATCGCGAAGGACATCGCGATGCATATCGCCGCGTCCAAGCCGAA
ATCGCTCGACGCCTCGGGCGTGCCGCAGGACCTGCTCGATACCGAGCGGCGCGTCGCGATCGAGAAGGCCAAGGAAGCCG
GCAAGCCGGAAGCGATGCTGGAAAAGATCGCTGAAGGCACGGTGCAGAAGTTCCTCAAGGAAGTCACGCTGCTCTCGCAG
CCCTTCGTCAAGGACGACAAGCAGACCGTCGAGCAGGTGCTGAAGTCGAAGAACTCGCAGTGCCACGGGTTCACGATGTA
CGTCGTCGGCGAGGGCATCGAGAAGAAGGTCTCCGACTTTGCTGCCGAGGTTGCCGCGGCCGCCAAGGTCTGA

Upstream 100 bases:

>100_bases
ACACGCCGGCCCCGGCCGGCGCGAGGTGTTTGAGGAGGGGCGTCGGCCCCTCTTTCTGTATTGGCTCAATTAATCGAGAA
ATCTGGGATAGGAGTTCGAA

Downstream 100 bases:

>100_bases
CGCAGTGGCGCCCGTTCGCCGCATCCTGCTGAAGCTCTCGGGCGAGGCCCTGATGGGACCGGACAGCTTCGGCTACCACG
CCGGGACGATGGCCGGTTTC

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MAEITASMVKDLREKTDAPMMDCKKALTEAGGDMQKAEEILRVRFGNKAAKSAGRVAAEGVVTIAISPDGKSAAMVEVNC
ETDFVAKNDDFLALSAALAEMVLTQKPADVAALSALPYKNSTVEGFRTELVGKIGENMSVRRFVRLDGQGKFASYIHGGK
IGVLVDVTGDEAIAKDIAMHIAASKPKSLDASGVPQDLLDTERRVAIEKAKEAGKPEAMLEKIAEGTVQKFLKEVTLLSQ
PFVKDDKQTVEQVLKSKNSQCHGFTMYVVGEGIEKKVSDFAAEVAAAAKV

Sequences:

>Translated_290_residues
MAEITASMVKDLREKTDAPMMDCKKALTEAGGDMQKAEEILRVRFGNKAAKSAGRVAAEGVVTIAISPDGKSAAMVEVNC
ETDFVAKNDDFLALSAALAEMVLTQKPADVAALSALPYKNSTVEGFRTELVGKIGENMSVRRFVRLDGQGKFASYIHGGK
IGVLVDVTGDEAIAKDIAMHIAASKPKSLDASGVPQDLLDTERRVAIEKAKEAGKPEAMLEKIAEGTVQKFLKEVTLLSQ
PFVKDDKQTVEQVLKSKNSQCHGFTMYVVGEGIEKKVSDFAAEVAAAAKV
>Mature_289_residues
AEITASMVKDLREKTDAPMMDCKKALTEAGGDMQKAEEILRVRFGNKAAKSAGRVAAEGVVTIAISPDGKSAAMVEVNCE
TDFVAKNDDFLALSAALAEMVLTQKPADVAALSALPYKNSTVEGFRTELVGKIGENMSVRRFVRLDGQGKFASYIHGGKI
GVLVDVTGDEAIAKDIAMHIAASKPKSLDASGVPQDLLDTERRVAIEKAKEAGKPEAMLEKIAEGTVQKFLKEVTLLSQP
FVKDDKQTVEQVLKSKNSQCHGFTMYVVGEGIEKKVSDFAAEVAAAAKV

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=224, Percent_Identity=31.25, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI291084500, Length=245, Percent_Identity=29.3877551020408, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI291084498, Length=108, Percent_Identity=39.8148148148148, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI291084502, Length=121, Percent_Identity=37.1900826446281, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786366, Length=290, Percent_Identity=48.2758620689655, Blast_Score=238, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17561440, Length=294, Percent_Identity=28.9115646258503, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19921466, Length=287, Percent_Identity=28.2229965156794, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_THIDA (Q3SKN8)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314544.1
- ProteinModelPortal:   Q3SKN8
- SMR:   Q3SKN8
- STRING:   Q3SKN8
- GeneID:   3673483
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0786
- NMPDR:   fig|292415.3.peg.50
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- PhylomeDB:   Q3SKN8
- ProtClustDB:   PRK09377
- BioCyc:   TDEN292415:TBD_0786-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 30981; Mature: 30850

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEITASMVKDLREKTDAPMMDCKKALTEAGGDMQKAEEILRVRFGNKAAKSAGRVAAEG
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCEEECC
VVTIAISPDGKSAAMVEVNCETDFVAKNDDFLALSAALAEMVLTQKPADVAALSALPYKN
EEEEEECCCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
STVEGFRTELVGKIGENMSVRRFVRLDGQGKFASYIHGGKIGVLVDVTGDEAIAKDIAMH
CCHHHHHHHHHHHHCCCCCEEHEEEECCCCCCHHHCCCCCEEEEEEECCCHHHHHHHHHH
IAASKPKSLDASGVPQDLLDTERRVAIEKAKEAGKPEAMLEKIAEGTVQKFLKEVTLLSQ
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PFVKDDKQTVEQVLKSKNSQCHGFTMYVVGEGIEKKVSDFAAEVAAAAKV
CCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEITASMVKDLREKTDAPMMDCKKALTEAGGDMQKAEEILRVRFGNKAAKSAGRVAAEG
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCEEECC
VVTIAISPDGKSAAMVEVNCETDFVAKNDDFLALSAALAEMVLTQKPADVAALSALPYKN
EEEEEECCCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
STVEGFRTELVGKIGENMSVRRFVRLDGQGKFASYIHGGKIGVLVDVTGDEAIAKDIAMH
CCHHHHHHHHHHHHCCCCCEEHEEEECCCCCCHHHCCCCCEEEEEEECCCHHHHHHHHHH
IAASKPKSLDASGVPQDLLDTERRVAIEKAKEAGKPEAMLEKIAEGTVQKFLKEVTLLSQ
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PFVKDDKQTVEQVLKSKNSQCHGFTMYVVGEGIEKKVSDFAAEVAAAAKV
CCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA