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The map label for this gene is pulD [H]

Identifier: 74316782

GI number: 74316782

Start: 807379

End: 809043

Strand: Direct

Name: pulD [H]

Synonym: Tbd_0764

Alternate gene names: 74316782

Gene position: 807379-809043 (Clockwise)

Preceding gene: 74316781

Following gene: 74316783

Centisome position: 27.75

GC content: 67.15

Gene sequence:

>1665_bases
ATGATGAAGACCCTATTGCTCGCTGCGCCGCTCGCGCTGGCCGGCTGCGCCGCGACGCTCACCGGGCAGGATACCCGGCA
GGCGATCCGCGACGACGTCGCGCAGGCCGCGCAGGCGAAGCCCGTTGCACCACCGGCCCTGAGCGACGATGAGGCCCGCG
CTGCGCTGATGCCGCCCATCAAGCTCGAGCGTCCGAAAGTTGCTGCGCCAGCCGAACCCCGCTTCGATCTCGCGGTCAGC
GGCGCGCCGGCGCAGGAGGTGTTCCTGGCGATCGTCTCCGGCACGCCCTACAGCATGCTCGTGCACCCGGAAGTCGCGGG
CTCGGTTTCGGCCAACCTCAAGAACGTGACCGTGCCCGAAGCGCTGTCTGCGATCCGCGAACTTTACGGCTACGAGTACA
CGATCGAGGGCCGCCGCGTCATGGTGCACCCGCTGACCCCGCAGACGCGGATCTTCAAGGTCAACTATCTGGCCGGAACC
CGCGCCGGTGCGTCGGACACGCGCGTGATCTCGGGCTCGGTCAGCGCCGCCTCGGGCAACGGCAACGGCGCGTCCGCAGG
CGGCAGCGGCAGCAGCAACCAGCAGTCGCTCGAAACGAGCAAGATCAGTACCGCGGTCAATTCGGATTTCTGGGGCGAAC
TGAAGCTCGCGCTCGACGCCATCGTCGGCGAATCGGGCAAGGTCATCCTGAGTCCGCAGTCCGGTGTCGTCGTCGTGAAG
ACGCCGCCGCGGACGATGCGCGAAGTCGAGCGTTTCCTCAAGGCGACCGCGCTCGCGGTCGAGCGCCAGGTCATGCTCGA
AGCAAAAATCATCGAGGTGCGGCTCAGCGACGGCTTCCAGACCGGAATCAACTGGGCGGCGCTGCACGAGGGGCGGCACA
AGTACTCGTCCGGCGCCGATGCGGGCAACTTCAATCTGGTGACGGGCGCGGCTTCGGGCTCGCTCGGCAGCGTCCTCGGC
AGCGGTTTGCCGGCCGCATCGGGAACCTCGGGCGGCATCTTCGGACTGGCGTTCCAGACCAAGAGCTTCGCCGCGCTGAT
CAATTTCCTCGAAACCCAGGGCGCGGTCCATGTCCTGTCGAGCCCGCGGATCGCCGCGCTGAACAACCAGAAGGCGGTGC
TCAAGGTCGGTACCGACGACTTCTTCGTGACCAACGTCTCCACGACGAGCAACACCGGCGGCGCGGGCACGACGACGACG
CCGAGCGTGACGCTGCAGCCCTTCTTCTCGGGGATCGCGCTCGACGTCACGCCGCAGATCGACGAGCGCGGGCGAATCAC
GCTGCATGTCCGTCCGTCGGTCAGCGTGGTGACCGAGAAAATCAAGAACATCGACGCCGGCGCCGCGGGCAGCCTCACGC
TGCCGCTCGCGTCGAGCGCGGTCTCGGAAACCGACAGCGTGGTGCAGGTCGAAGACGGCCGCATCGTCGCGATCGGCGGC
ATGATGAAACAGTCGTTCGATGACAGCGCCAACCGTGTTCCCGCGATCGGACGCGTGCCGCTGCTCGGTTACTTCTTCGG
CAACACCGCGCGCAGCGCGAGCAAGAGCGAGCTCGTGATCCTGATCAAGCCCACCGTCATCGACAGCCAGGAAGACTGGC
AGGCCGACGCGGAAGCGACGCGGGCGCGTCTCGAGAAGCTCGAGCGGCAAGCCGCGAATCCGTGA

Upstream 100 bases:

>100_bases
AGGTCGTGCTGCGCCGCGGCGGTGAAACCACGGTGCTCAGACTCTACCCGCAGGTCGAAAAACGGCCGCGCGGGAAATAA
GAAAACAGGAGACATTGCGG

Downstream 100 bases:

>100_bases
TCGCGTCGTCCGCGCGTCCTGACAACGCGTCGATCGTCGATGCGGGCAGGCGGGCGGTCGGCGCGGGCAGCGGGCTGTCC
TATCTTGCGCATTTCGGGTT

Product: general secretion pathway protein D

Products: NA

Alternate protein names: Pullulanase secretion envelope pulD [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MMKTLLLAAPLALAGCAATLTGQDTRQAIRDDVAQAAQAKPVAPPALSDDEARAALMPPIKLERPKVAAPAEPRFDLAVS
GAPAQEVFLAIVSGTPYSMLVHPEVAGSVSANLKNVTVPEALSAIRELYGYEYTIEGRRVMVHPLTPQTRIFKVNYLAGT
RAGASDTRVISGSVSAASGNGNGASAGGSGSSNQQSLETSKISTAVNSDFWGELKLALDAIVGESGKVILSPQSGVVVVK
TPPRTMREVERFLKATALAVERQVMLEAKIIEVRLSDGFQTGINWAALHEGRHKYSSGADAGNFNLVTGAASGSLGSVLG
SGLPAASGTSGGIFGLAFQTKSFAALINFLETQGAVHVLSSPRIAALNNQKAVLKVGTDDFFVTNVSTTSNTGGAGTTTT
PSVTLQPFFSGIALDVTPQIDERGRITLHVRPSVSVVTEKIKNIDAGAAGSLTLPLASSAVSETDSVVQVEDGRIVAIGG
MMKQSFDDSANRVPAIGRVPLLGYFFGNTARSASKSELVILIKPTVIDSQEDWQADAEATRARLEKLERQAANP

Sequences:

>Translated_554_residues
MMKTLLLAAPLALAGCAATLTGQDTRQAIRDDVAQAAQAKPVAPPALSDDEARAALMPPIKLERPKVAAPAEPRFDLAVS
GAPAQEVFLAIVSGTPYSMLVHPEVAGSVSANLKNVTVPEALSAIRELYGYEYTIEGRRVMVHPLTPQTRIFKVNYLAGT
RAGASDTRVISGSVSAASGNGNGASAGGSGSSNQQSLETSKISTAVNSDFWGELKLALDAIVGESGKVILSPQSGVVVVK
TPPRTMREVERFLKATALAVERQVMLEAKIIEVRLSDGFQTGINWAALHEGRHKYSSGADAGNFNLVTGAASGSLGSVLG
SGLPAASGTSGGIFGLAFQTKSFAALINFLETQGAVHVLSSPRIAALNNQKAVLKVGTDDFFVTNVSTTSNTGGAGTTTT
PSVTLQPFFSGIALDVTPQIDERGRITLHVRPSVSVVTEKIKNIDAGAAGSLTLPLASSAVSETDSVVQVEDGRIVAIGG
MMKQSFDDSANRVPAIGRVPLLGYFFGNTARSASKSELVILIKPTVIDSQEDWQADAEATRARLEKLERQAANP
>Mature_554_residues
MMKTLLLAAPLALAGCAATLTGQDTRQAIRDDVAQAAQAKPVAPPALSDDEARAALMPPIKLERPKVAAPAEPRFDLAVS
GAPAQEVFLAIVSGTPYSMLVHPEVAGSVSANLKNVTVPEALSAIRELYGYEYTIEGRRVMVHPLTPQTRIFKVNYLAGT
RAGASDTRVISGSVSAASGNGNGASAGGSGSSNQQSLETSKISTAVNSDFWGELKLALDAIVGESGKVILSPQSGVVVVK
TPPRTMREVERFLKATALAVERQVMLEAKIIEVRLSDGFQTGINWAALHEGRHKYSSGADAGNFNLVTGAASGSLGSVLG
SGLPAASGTSGGIFGLAFQTKSFAALINFLETQGAVHVLSSPRIAALNNQKAVLKVGTDDFFVTNVSTTSNTGGAGTTTT
PSVTLQPFFSGIALDVTPQIDERGRITLHVRPSVSVVTEKIKNIDAGAAGSLTLPLASSAVSETDSVVQVEDGRIVAIGG
MMKQSFDDSANRVPAIGRVPLLGYFFGNTARSASKSELVILIKPTVIDSQEDWQADAEATRARLEKLERQAANP

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=467, Percent_Identity=22.4839400428266, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI87082242, Length=295, Percent_Identity=25.7627118644068, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 57689; Mature: 57689

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKTLLLAAPLALAGCAATLTGQDTRQAIRDDVAQAAQAKPVAPPALSDDEARAALMPPI
CCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCC
KLERPKVAAPAEPRFDLAVSGAPAQEVFLAIVSGTPYSMLVHPEVAGSVSANLKNVTVPE
CCCCCCCCCCCCCCEEEEECCCCHHHEEEEEECCCCEEEEECCCCCCCCCCCCCCCCHHH
ALSAIRELYGYEYTIEGRRVMVHPLTPQTRIFKVNYLAGTRAGASDTRVISGSVSAASGN
HHHHHHHHHCCEEEECCCEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEECCCEECCCC
GNGASAGGSGSSNQQSLETSKISTAVNSDFWGELKLALDAIVGESGKVILSPQSGVVVVK
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEEE
TPPRTMREVERFLKATALAVERQVMLEAKIIEVRLSDGFQTGINWAALHEGRHKYSSGAD
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCEEEECCCCHHCCCCCC
AGNFNLVTGAASGSLGSVLGSGLPAASGTSGGIFGLAFQTKSFAALINFLETQGAVHVLS
CCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEC
SPRIAALNNQKAVLKVGTDDFFVTNVSTTSNTGGAGTTTTPSVTLQPFFSGIALDVTPQI
CCCEEEECCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCC
DERGRITLHVRPSVSVVTEKIKNIDAGAAGSLTLPLASSAVSETDSVVQVEDGRIVAIGG
CCCCEEEEEECCCHHHHHHHHHCCCCCCCCCEEECCCHHHHCCCCCEEEECCCEEEEECC
MMKQSFDDSANRVPAIGRVPLLGYFFGNTARSASKSELVILIKPTVIDSQEDWQADAEAT
HHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECEEECCCHHCCHHHHHH
RARLEKLERQAANP
HHHHHHHHHHHCCC
>Mature Secondary Structure
MMKTLLLAAPLALAGCAATLTGQDTRQAIRDDVAQAAQAKPVAPPALSDDEARAALMPPI
CCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCC
KLERPKVAAPAEPRFDLAVSGAPAQEVFLAIVSGTPYSMLVHPEVAGSVSANLKNVTVPE
CCCCCCCCCCCCCCEEEEECCCCHHHEEEEEECCCCEEEEECCCCCCCCCCCCCCCCHHH
ALSAIRELYGYEYTIEGRRVMVHPLTPQTRIFKVNYLAGTRAGASDTRVISGSVSAASGN
HHHHHHHHHCCEEEECCCEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEECCCEECCCC
GNGASAGGSGSSNQQSLETSKISTAVNSDFWGELKLALDAIVGESGKVILSPQSGVVVVK
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEEE
TPPRTMREVERFLKATALAVERQVMLEAKIIEVRLSDGFQTGINWAALHEGRHKYSSGAD
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCEEEECCCCHHCCCCCC
AGNFNLVTGAASGSLGSVLGSGLPAASGTSGGIFGLAFQTKSFAALINFLETQGAVHVLS
CCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEC
SPRIAALNNQKAVLKVGTDDFFVTNVSTTSNTGGAGTTTTPSVTLQPFFSGIALDVTPQI
CCCEEEECCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCC
DERGRITLHVRPSVSVVTEKIKNIDAGAAGSLTLPLASSAVSETDSVVQVEDGRIVAIGG
CCCCEEEEEECCCHHHHHHHHHCCCCCCCCCEEECCCHHHHCCCCCEEEECCCEEEEECC
MMKQSFDDSANRVPAIGRVPLLGYFFGNTARSASKSELVILIKPTVIDSQEDWQADAEAT
HHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECEEECCCHHCCHHHHHH
RARLEKLERQAANP
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2677007 [H]