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The map label for this gene is carB [C]

Identifier: 74316739

GI number: 74316739

Start: 762443

End: 763651

Strand: Reverse

Name: carB [C]

Synonym: Tbd_0721

Alternate gene names: 74316739

Gene position: 763651-762443 (Counterclockwise)

Preceding gene: 74316740

Following gene: 74316738

Centisome position: 26.24

GC content: 68.9

Gene sequence:

>1209_bases
ATGCGAGAGCTCATCCTGCTGGCCCACGTGCCGACCGATTCGGTCAACGACGGCTTCATTCCGGCAGCGCAGCGGCGCGG
CCTGTCGGTCGTGCTGCTGACCGACCACGCCGAGGCGCACCGGCAGCATTTCAGCCGGGCCGGCCTTGCCGCCTATCCCC
ACGAAATCGTCGCCTGCGACGTGTTCAATCCGCTCGCGGTCGTCGACGCGATCACCTGCCGGGCCGAGGCGCCCACAGCG
GTGTTTTCCAACAGCGACCACCTGCAGACGAGCGCGGCGATCGCCGCCGAATACTTCGACCTGCCGCGCAAGGACTGGCA
CGTCACCTATCGCGCGAAGAACAAGGCGGAAATGCGCGCCCGGCTCAAGGCGCGCGGCCTCGACACGCTCTGGCATGCCG
TGGTCTGCGACGCGGCAGGGCTCGCGCGCGTCGTCGACGGCGCGCCTTTACCCTGCGTCGTCAAGCCGCGCGAAGGCGTG
GCCAGCCAGCAGGTCAGTCTCGCCCATGACCGCGCCGAACTCGCCGCGCAGTGCGCCGCCGCCTGGTCCAGCCATCCGGG
CCAGCCGCTGCTGCTCGAGGAATACGTCGCGGGTCCGCTCTACACGCTGGAAACCCTCGGCGACGACAAGCGGCTCTGCG
TGCTCGGCGGCTTCCAGGTCAGGCTGTCGCCGCCGCCGCATTTCGTCGAGCTCGAGGCGCGCTGGGGAACGGGTCTGACC
GCCGAGCAGGAGGCGCAGGTCATCGACCTCGTAAGGCATTTCGGCGTCGGCTTCGGCGCCTGCCACACCGAGTTCGTGAT
GACCGACGCCGGCCCGAAGCTGATCGAGATCAACTACCGCAGCATCGGCGACTACCGCGAGTTCCTGCTGCAGGACGCGC
TCGGCCTGCCCTTGTTCGAGATCATTCTCGATCTCTACGCCGGTCAGCCGCTGCCCGACATCGACGTTGGGCCGGGCGCG
GCGCTGATCCGCTATTTCACCGCGCAGTCGGCCGGGCGCTTGACCGGCGCGCCGGATGCCTTCGACCGGCGCTCGGAGGA
CGTGCAGATCACGTTCAAGCCATTGCGCGCGATCGGCGAGACCGTCAATCTCACCCACTCCAACAAGGACTACCTCGGCG
TGCTGCGCGGCACGGGCGCCGACGTCCAGCGGCTGGGGCTCGAGATGGACCGGCTCAGCAGCGGCCTCGCGTGGACGATC
GCGGCATGA

Upstream 100 bases:

>100_bases
ACTATTACTTCCGCGGCGTCGACTTCAGCCAGGGCCGCATGCCGGCGCCGGGCCGCGCCGCCTTGCTCACGCTACAGATG
GACATGTGAGGAGGGCGACC

Downstream 100 bases:

>100_bases
ACGCGCGCGCCCCGAAGACCGCCCTCGCCGCCGCCCCGTCACGCAACGACGACGCGTCGCCGGAGGCCTACATCTGCCGG
CGCGTCGTCGACGCCCTGCT

Product: hypothetical protein

Products: ADP; Carbamoyl phosphate; L-Glutamate; H+; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MRELILLAHVPTDSVNDGFIPAAQRRGLSVVLLTDHAEAHRQHFSRAGLAAYPHEIVACDVFNPLAVVDAITCRAEAPTA
VFSNSDHLQTSAAIAAEYFDLPRKDWHVTYRAKNKAEMRARLKARGLDTLWHAVVCDAAGLARVVDGAPLPCVVKPREGV
ASQQVSLAHDRAELAAQCAAAWSSHPGQPLLLEEYVAGPLYTLETLGDDKRLCVLGGFQVRLSPPPHFVELEARWGTGLT
AEQEAQVIDLVRHFGVGFGACHTEFVMTDAGPKLIEINYRSIGDYREFLLQDALGLPLFEIILDLYAGQPLPDIDVGPGA
ALIRYFTAQSAGRLTGAPDAFDRRSEDVQITFKPLRAIGETVNLTHSNKDYLGVLRGTGADVQRLGLEMDRLSSGLAWTI
AA

Sequences:

>Translated_402_residues
MRELILLAHVPTDSVNDGFIPAAQRRGLSVVLLTDHAEAHRQHFSRAGLAAYPHEIVACDVFNPLAVVDAITCRAEAPTA
VFSNSDHLQTSAAIAAEYFDLPRKDWHVTYRAKNKAEMRARLKARGLDTLWHAVVCDAAGLARVVDGAPLPCVVKPREGV
ASQQVSLAHDRAELAAQCAAAWSSHPGQPLLLEEYVAGPLYTLETLGDDKRLCVLGGFQVRLSPPPHFVELEARWGTGLT
AEQEAQVIDLVRHFGVGFGACHTEFVMTDAGPKLIEINYRSIGDYREFLLQDALGLPLFEIILDLYAGQPLPDIDVGPGA
ALIRYFTAQSAGRLTGAPDAFDRRSEDVQITFKPLRAIGETVNLTHSNKDYLGVLRGTGADVQRLGLEMDRLSSGLAWTI
AA
>Mature_402_residues
MRELILLAHVPTDSVNDGFIPAAQRRGLSVVLLTDHAEAHRQHFSRAGLAAYPHEIVACDVFNPLAVVDAITCRAEAPTA
VFSNSDHLQTSAAIAAEYFDLPRKDWHVTYRAKNKAEMRARLKARGLDTLWHAVVCDAAGLARVVDGAPLPCVVKPREGV
ASQQVSLAHDRAELAAQCAAAWSSHPGQPLLLEEYVAGPLYTLETLGDDKRLCVLGGFQVRLSPPPHFVELEARWGTGLT
AEQEAQVIDLVRHFGVGFGACHTEFVMTDAGPKLIEINYRSIGDYREFLLQDALGLPLFEIILDLYAGQPLPDIDVGPGA
ALIRYFTAQSAGRLTGAPDAFDRRSEDVQITFKPLRAIGETVNLTHSNKDYLGVLRGTGADVQRLGLEMDRLSSGLAWTI
AA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005479 [H]

Pfam domain/function: PF02786 CPSase_L_D2 [H]

EC number: 6.3.5.5 [C]

Molecular weight: Translated: 43712; Mature: 43712

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50975 ATP_GRASP ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRELILLAHVPTDSVNDGFIPAAQRRGLSVVLLTDHAEAHRQHFSRAGLAAYPHEIVACD
CCCEEEEEECCCCCCCCCCCCHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCCCEEEEC
VFNPLAVVDAITCRAEAPTAVFSNSDHLQTSAAIAAEYFDLPRKDWHVTYRAKNKAEMRA
CCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHH
RLKARGLDTLWHAVVCDAAGLARVVDGAPLPCVVKPREGVASQQVSLAHDRAELAAQCAA
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
AWSSHPGQPLLLEEYVAGPLYTLETLGDDKRLCVLGGFQVRLSPPPHFVELEARWGTGLT
HHCCCCCCCEEHHHHHCCCEEEEECCCCCCEEEEEECEEEEECCCCCEEEEEECCCCCCC
AEQEAQVIDLVRHFGVGFGACHTEFVMTDAGPKLIEINYRSIGDYREFLLQDALGLPLFE
CHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCHHH
IILDLYAGQPLPDIDVGPGAALIRYFTAQSAGRLTGAPDAFDRRSEDVQITFKPLRAIGE
HHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEEEHHHHHCCC
TVNLTHSNKDYLGVLRGTGADVQRLGLEMDRLSSGLAWTIAA
EEEEEECCCCEEEEEECCCCCHHHHCCCHHHHCCCCEEEEEC
>Mature Secondary Structure
MRELILLAHVPTDSVNDGFIPAAQRRGLSVVLLTDHAEAHRQHFSRAGLAAYPHEIVACD
CCCEEEEEECCCCCCCCCCCCHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCCCEEEEC
VFNPLAVVDAITCRAEAPTAVFSNSDHLQTSAAIAAEYFDLPRKDWHVTYRAKNKAEMRA
CCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHH
RLKARGLDTLWHAVVCDAAGLARVVDGAPLPCVVKPREGVASQQVSLAHDRAELAAQCAA
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
AWSSHPGQPLLLEEYVAGPLYTLETLGDDKRLCVLGGFQVRLSPPPHFVELEARWGTGLT
HHCCCCCCCEEHHHHHCCCEEEEECCCCCCEEEEEECEEEEECCCCCEEEEEECCCCCCC
AEQEAQVIDLVRHFGVGFGACHTEFVMTDAGPKLIEINYRSIGDYREFLLQDALGLPLFE
CHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCHHH
IILDLYAGQPLPDIDVGPGAALIRYFTAQSAGRLTGAPDAFDRRSEDVQITFKPLRAIGE
HHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEEEHHHHHCCC
TVNLTHSNKDYLGVLRGTGADVQRLGLEMDRLSSGLAWTIAA
EEEEEECCCCEEEEEECCCCCHHHHCCCHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; GDP; GMP; GTP; IDP; IMP; ITP; N-Acetylglutamate; NEM; Orn; Thiol; XMP [C]

Metal ions: K+; Mg2+; Mn2+; NH4+ [C]

Kcat value (1/min): 120000 [C]

Specific activity: NA

Km value (mM): 93 {NH4+}} 0.38 {L-Gln}} [C]

Substrates: ATP; L-Glutamine; H2O; Bicarbonate [C]

Specific reaction: (2) ATP + L-Glutamine + H2O + Bicarbonate --> (2) ADP + Carbamoyl phosphate + L-Glutamate + (2) H+ + Phosphate [C]

General reaction: Amide group transfer; Amination; Phosphorylation [C]

Inhibitor: 6-Diazo-5-oxon/leucine; Alkyl hydrazines; Azaserine; Guanidine; H2O2; NH2OH; L-2-Amino-4-oxo-5-chloropentanoate; L-Cyanate; NEM; P1, P5-Di (adenosine 5')-pentaphosphate; UDP; UMP phosphate; Urea; UTP [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]