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The map label for this gene is cspA [H]

Identifier: 74316697

GI number: 74316697

Start: 720925

End: 721131

Strand: Direct

Name: cspA [H]

Synonym: Tbd_0679

Alternate gene names: 74316697

Gene position: 720925-721131 (Clockwise)

Preceding gene: 74316696

Following gene: 74316698

Centisome position: 24.78

GC content: 54.59

Gene sequence:

>207_bases
ATGGAAACGGGTACTGTCAAGTGGTTCAACGATGCAAAAGGTTTTGGTTTCATCACGCCCGACAACGGCGGTGAAGACCT
CTTTGCGCATTTCTCCGCAATCAACGCCAACGGCTTCAAGTCCCTGCAGGAAAACCAGCGCGTCAGCTTCGAAGTGACGA
CTGGCCCCAAGGGCAAGCAGGCGTCGAACATCCAGGTCGTGTCGTAA

Upstream 100 bases:

>100_bases
GGATTCAAGCTTTAAATTGCGGTACCTCTGGTAAGGCTTTTTCCTTGAAATTCGATTGATAGGGCATGTCGCCCAATTTT
TTAGATAGATAGGAATGAAG

Downstream 100 bases:

>100_bases
TCCTTCCGACCTGATGTGAAAAACCCCGGTTCGCCGGGGTTTTTTTATGGGTATTCCCGAAATTTACGGCCGTCGTGCGG
TGTCCTACGCCGCGGTGTGC

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 68; Mature: 68

Protein sequence:

>68_residues
METGTVKWFNDAKGFGFITPDNGGEDLFAHFSAINANGFKSLQENQRVSFEVTTGPKGKQASNIQVVS

Sequences:

>Translated_68_residues
METGTVKWFNDAKGFGFITPDNGGEDLFAHFSAINANGFKSLQENQRVSFEVTTGPKGKQASNIQVVS
>Mature_68_residues
METGTVKWFNDAKGFGFITPDNGGEDLFAHFSAINANGFKSLQENQRVSFEVTTGPKGKQASNIQVVS

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=60.9375, Blast_Score=89, Evalue=4e-20,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=63.9344262295082, Blast_Score=89, Evalue=5e-20,
Organism=Escherichia coli, GI1789979, Length=62, Percent_Identity=61.2903225806452, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=56.4516129032258, Blast_Score=82, Evalue=9e-18,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=57.8125, Blast_Score=82, Evalue=9e-18,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=59.6774193548387, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI1787839, Length=64, Percent_Identity=50, Blast_Score=74, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71983225, Length=56, Percent_Identity=50, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71983217, Length=56, Percent_Identity=50, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24658520, Length=61, Percent_Identity=45.9016393442623, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7367; Mature: 7367

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METGTVKWFNDAKGFGFITPDNGGEDLFAHFSAINANGFKSLQENQRVSFEVTTGPKGKQ
CCCCCEEEECCCCCCEEECCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCC
ASNIQVVS
CCCEEEEC
>Mature Secondary Structure
METGTVKWFNDAKGFGFITPDNGGEDLFAHFSAINANGFKSLQENQRVSFEVTTGPKGKQ
CCCCCEEEECCCCCCEEECCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCC
ASNIQVVS
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]