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The map label for this gene is eno

Identifier: 74316639

GI number: 74316639

Start: 653172

End: 654455

Strand: Direct

Name: eno

Synonym: Tbd_0621

Alternate gene names: 74316639

Gene position: 653172-654455 (Clockwise)

Preceding gene: 74316638

Following gene: 74316640

Centisome position: 22.45

GC content: 63.79

Gene sequence:

>1284_bases
TTGAGCGCCATTATCGATGTCATCGCCCGGGAAATTCTCGACTCCCGCGGCAACCCCACTGTTGAAGCCGACGTGCTGCT
CGAGTCCGGCGTGATCGGGCGCGCCGCCGTGCCCTCGGGCGCGTCGACCGGCTCGCGCGAGGCGATCGAACTGCGCGACG
GCGACAAGTCGCGCTACCTCGGCAAGGGCGTGTTGAAGGCGGTCGAGCACGTCAACACCGAAATCTGCGAGGCGATCATC
GGCCTTGATGTCGAAGACCAGGCCTTCATCGACCAGACCATGATCGAACTCGACGGCACCGAGAACAAATCGCGTCTCGG
CGCCAACGCGCTGCTCGCGGTATCGATGGCGTGCGCGCGCGCCGCCGCCGAGCAGGCGGGCCTGCCGCTGTACCGCTATC
TGGGCGGCGCCGCACCGATGCAACTCCCGGTGCCGATGATGAACATCATCAACGGCGGCGCCCACGCCAACAACAACATC
GACATGCAGGAATTCATGGTGATTCCGGTCGGCGCGCCGAGCTTCCGCGAGGCCTTGCGCTACGGTGCCGAAGTCTTCCA
CGCGCTGAAGAAGCTCCTGGACGACGCGGGTATGACGACGACGGTCGGCGATGAGGGCGGGTTCGCTCCCAACCTCGAGT
CGCACGAGGCCGCACTAAAGCTGATCGTGCAGGCCATCGAGAAAGCCGGGCTGCAGCCCGGCATCGACGTCGCGATCGGC
GTCGACTGTGCGAGCTCGGAGTTCTACAAGGACGGCCTCTATCACATCGATTCCGAAAACCTCAAGCTCACGTCGGCCGC
GTTCACCGACTATCTCGCCGCCTGGTGCGACAAGTACCCGATCATCAGCATCGAGGACGGCATGGCCGAGGGCGACTGGG
ACGGCTGGAAGATCCTGACCGACCGGCTCGGCCGACGCGTGCAGTTGGTCGGCGACGATCTTTTCGTCACCAACGCGAAA
ATTCTCAAGGAAGGCATCGAAAAGGACATCGCCAACTCGATCCTGATCAAGGTCAACCAGATCGGCACCTTGTCCGAGAC
TTTCCAGGCGATCGAGATGGCCAAGCAGGCGGGCTACACCTCGGTGATCTCCCATCGTTCGGGCGAGACCGAGGACACGA
CGATCGCCGATCTCGCCGTCGCGACCAATGCGCGCCAGATCAAGACCGGCTCGCTGTCGCGCTCCGACCGCATGGCCAAA
TACAACCAGCTGCTGCGCATCGAAGAAGAACTCGGCGACACGGCAAGCTACCCCGGCCGCGATGCTTTCCGCCAGCGCGG
CTAA

Upstream 100 bases:

>100_bases
TTTATTGAACACAAGCTGATGAAGGCTTGAGCGCCGCGGCAGGCACGGGCTCGCGGCCCGTGCTGTTGCAGCGCGGACTG
ATAAAGACTGAGGAAAAAGA

Downstream 100 bases:

>100_bases
CCGGATTGCACGCATTCGAAGACGCAGCCTCACCTGGGGCCTGGCGGGCGCTGTCGTGTTGCTGCAATACCCGCTGTGGC
TCGGCGAGGGCGGCTGGTTG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSAIIDVIAREILDSRGNPTVEADVLLESGVIGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHVNTEICEAII
GLDVEDQAFIDQTMIELDGTENKSRLGANALLAVSMACARAAAEQAGLPLYRYLGGAAPMQLPVPMMNIINGGAHANNNI
DMQEFMVIPVGAPSFREALRYGAEVFHALKKLLDDAGMTTTVGDEGGFAPNLESHEAALKLIVQAIEKAGLQPGIDVAIG
VDCASSEFYKDGLYHIDSENLKLTSAAFTDYLAAWCDKYPIISIEDGMAEGDWDGWKILTDRLGRRVQLVGDDLFVTNAK
ILKEGIEKDIANSILIKVNQIGTLSETFQAIEMAKQAGYTSVISHRSGETEDTTIADLAVATNARQIKTGSLSRSDRMAK
YNQLLRIEEELGDTASYPGRDAFRQRG

Sequences:

>Translated_427_residues
MSAIIDVIAREILDSRGNPTVEADVLLESGVIGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHVNTEICEAII
GLDVEDQAFIDQTMIELDGTENKSRLGANALLAVSMACARAAAEQAGLPLYRYLGGAAPMQLPVPMMNIINGGAHANNNI
DMQEFMVIPVGAPSFREALRYGAEVFHALKKLLDDAGMTTTVGDEGGFAPNLESHEAALKLIVQAIEKAGLQPGIDVAIG
VDCASSEFYKDGLYHIDSENLKLTSAAFTDYLAAWCDKYPIISIEDGMAEGDWDGWKILTDRLGRRVQLVGDDLFVTNAK
ILKEGIEKDIANSILIKVNQIGTLSETFQAIEMAKQAGYTSVISHRSGETEDTTIADLAVATNARQIKTGSLSRSDRMAK
YNQLLRIEEELGDTASYPGRDAFRQRG
>Mature_426_residues
SAIIDVIAREILDSRGNPTVEADVLLESGVIGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHVNTEICEAIIG
LDVEDQAFIDQTMIELDGTENKSRLGANALLAVSMACARAAAEQAGLPLYRYLGGAAPMQLPVPMMNIINGGAHANNNID
MQEFMVIPVGAPSFREALRYGAEVFHALKKLLDDAGMTTTVGDEGGFAPNLESHEAALKLIVQAIEKAGLQPGIDVAIGV
DCASSEFYKDGLYHIDSENLKLTSAAFTDYLAAWCDKYPIISIEDGMAEGDWDGWKILTDRLGRRVQLVGDDLFVTNAKI
LKEGIEKDIANSILIKVNQIGTLSETFQAIEMAKQAGYTSVISHRSGETEDTTIADLAVATNARQIKTGSLSRSDRMAKY
NQLLRIEEELGDTASYPGRDAFRQRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=435, Percent_Identity=53.3333333333333, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=435, Percent_Identity=50.5747126436782, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897477, Length=438, Percent_Identity=51.5981735159817, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=438, Percent_Identity=51.5981735159817, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI301897479, Length=436, Percent_Identity=46.1009174311927, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=25.8928571428571, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=25.8928571428571, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=25.8928571428571, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI310129182, Length=207, Percent_Identity=26.5700483091787, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310110045, Length=207, Percent_Identity=26.5700483091787, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310120572, Length=207, Percent_Identity=26.5700483091787, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789141, Length=429, Percent_Identity=63.8694638694639, Blast_Score=541, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71995829, Length=435, Percent_Identity=51.9540229885057, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17536383, Length=435, Percent_Identity=51.9540229885057, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32563855, Length=195, Percent_Identity=46.6666666666667, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=51.1520737327189, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=50.3464203233256, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=50.3464203233256, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=50.3464203233256, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=50.6912442396313, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=51.3888888888889, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=51.3888888888889, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=51.3888888888889, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=51.3888888888889, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=51.3888888888889, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=51.3888888888889, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_THIDA (Q3SL43)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314379.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q3SL43
- SMR:   Q3SL43
- STRING:   Q3SL43
- GeneID:   3673732
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0621
- NMPDR:   fig|292415.3.peg.508
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q3SL43
- ProtClustDB:   PRK00077
- BioCyc:   TDEN292415:TBD_0621-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45984; Mature: 45852

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIIDVIAREILDSRGNPTVEADVLLESGVIGRAAVPSGASTGSREAIELRDGDKSRYL
CHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEHVNTEICEAIIGLDVEDQAFIDQTMIELDGTENKSRLGANALLAVSMACAR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCCCCHHHHCHHHHHHHHHHHHH
AAAEQAGLPLYRYLGGAAPMQLPVPMMNIINGGAHANNNIDMQEFMVIPVGAPSFREALR
HHHHHCCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
YGAEVFHALKKLLDDAGMTTTVGDEGGFAPNLESHEAALKLIVQAIEKAGLQPGIDVAIG
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
VDCASSEFYKDGLYHIDSENLKLTSAAFTDYLAAWCDKYPIISIEDGMAEGDWDGWKILT
CCCCCCHHHHCCCEEECCCCEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHH
DRLGRRVQLVGDDLFVTNAKILKEGIEKDIANSILIKVNQIGTLSETFQAIEMAKQAGYT
HHHCCEEEEECCCEEEHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCHH
SVISHRSGETEDTTIADLAVATNARQIKTGSLSRSDRMAKYNQLLRIEEELGDTASYPGR
HHHHCCCCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
DAFRQRG
HHHHCCC
>Mature Secondary Structure 
SAIIDVIAREILDSRGNPTVEADVLLESGVIGRAAVPSGASTGSREAIELRDGDKSRYL
HHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEHVNTEICEAIIGLDVEDQAFIDQTMIELDGTENKSRLGANALLAVSMACAR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCCCCHHHHCHHHHHHHHHHHHH
AAAEQAGLPLYRYLGGAAPMQLPVPMMNIINGGAHANNNIDMQEFMVIPVGAPSFREALR
HHHHHCCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
YGAEVFHALKKLLDDAGMTTTVGDEGGFAPNLESHEAALKLIVQAIEKAGLQPGIDVAIG
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
VDCASSEFYKDGLYHIDSENLKLTSAAFTDYLAAWCDKYPIISIEDGMAEGDWDGWKILT
CCCCCCHHHHCCCEEECCCCEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHH
DRLGRRVQLVGDDLFVTNAKILKEGIEKDIANSILIKVNQIGTLSETFQAIEMAKQAGYT
HHHCCEEEEECCCEEEHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCHH
SVISHRSGETEDTTIADLAVATNARQIKTGSLSRSDRMAKYNQLLRIEEELGDTASYPGR
HHHHCCCCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
DAFRQRG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA