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The map label for this gene is rlmB [H]

Identifier: 74316627

GI number: 74316627

Start: 638542

End: 639306

Strand: Direct

Name: rlmB [H]

Synonym: Tbd_0609

Alternate gene names: 74316627

Gene position: 638542-639306 (Clockwise)

Preceding gene: 74316626

Following gene: 74316628

Centisome position: 21.94

GC content: 69.28

Gene sequence:

>765_bases
ATGAGCGAGAAGCCTGCCGAAAGACTCATCTACGGTTTCCATGCCGTGCTCGCCCGGCTCCGGCACGATCCGGCGGGCGT
GCTCGAAATCTACCTCGATCTCACGCGGCGTGACGCCCGCGCCCGCGATCTCGTGCATCAGGCGCGCGACAGCGGAGTCA
AGCTCGCCCAGGTCGAGGCCGACCGTCTCGGGCGCCTGGTCGGCAAGGCCGCCAAGCACCAGGGCGTCGTCGCCCGCGTC
AAGCCGGTCGCGCTGACGCACTCGCTCGACGAAGTGCTCGAAAGCATCGAAGGCCCGCCGCTTCTCGTCATCCTCGACGG
CGTCACGGATCCGCACAACCTCGGCGCGATCCTGCGCTCGGCCGACGCCTTCGGCGCGCATGCCGTCATCGCGCCGAAGG
ACAACGCGGTCGGCATCAACGCCACGGTCGAGAAGGTCGCCTCGGGCGCGGCGGACACCGTCCCCTACCTCATGGTGACC
AACCTCGCGCGCACGATCGAGGAGTTGAAGGAACACGACATCTGGGTCATCGCGGCCGACATGGACGGCGAGCAGCCGCT
GTCCGGCATCGACGTCAGGAGCGGCGTCGCATGGGTGCTCGGCGCCGAGGGCGCGGGCATCCGCCGCCTGGTCAAGCAGC
GTTGCGATCAGGTCGCGCGCATCCCCATCGGCGGCACGGTCGAGAGCCTCAACGTGTCGGTCTCGGCGGCGCTCTGCCTG
TACGAGACCGCCCGGCAACGAGGACACGCGTCGACGCGGCAGTAG

Upstream 100 bases:

>100_bases
ACTTCAGCCTCGTCGAGACGATCGAGACTGCGACCCAGCCCGAGGAGAGCGTCGCGCCGAAGAAGAAACGCAGCAAGACA
CGCGGCGGCGGCAAGAAGAA

Downstream 100 bases:

>100_bases
ACGGCGGCAGCTGCGCCGTCGCGCGGGGGCAGTCCTCCGATTCCGACTCCAAAAACGGACTATGTTGAAAGCTTGTCCGG
CTCGTCAAACGAAGTCCGGG

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSEKPAERLIYGFHAVLARLRHDPAGVLEIYLDLTRRDARARDLVHQARDSGVKLAQVEADRLGRLVGKAAKHQGVVARV
KPVALTHSLDEVLESIEGPPLLVILDGVTDPHNLGAILRSADAFGAHAVIAPKDNAVGINATVEKVASGAADTVPYLMVT
NLARTIEELKEHDIWVIAADMDGEQPLSGIDVRSGVAWVLGAEGAGIRRLVKQRCDQVARIPIGGTVESLNVSVSAALCL
YETARQRGHASTRQ

Sequences:

>Translated_254_residues
MSEKPAERLIYGFHAVLARLRHDPAGVLEIYLDLTRRDARARDLVHQARDSGVKLAQVEADRLGRLVGKAAKHQGVVARV
KPVALTHSLDEVLESIEGPPLLVILDGVTDPHNLGAILRSADAFGAHAVIAPKDNAVGINATVEKVASGAADTVPYLMVT
NLARTIEELKEHDIWVIAADMDGEQPLSGIDVRSGVAWVLGAEGAGIRRLVKQRCDQVARIPIGGTVESLNVSVSAALCL
YETARQRGHASTRQ
>Mature_253_residues
SEKPAERLIYGFHAVLARLRHDPAGVLEIYLDLTRRDARARDLVHQARDSGVKLAQVEADRLGRLVGKAAKHQGVVARVK
PVALTHSLDEVLESIEGPPLLVILDGVTDPHNLGAILRSADAFGAHAVIAPKDNAVGINATVEKVASGAADTVPYLMVTN
LARTIEELKEHDIWVIAADMDGEQPLSGIDVRSGVAWVLGAEGAGIRRLVKQRCDQVARIPIGGTVESLNVSVSAALCLY
ETARQRGHASTRQ

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=196, Percent_Identity=31.1224489795918, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=45.2282157676349, Blast_Score=203, Evalue=9e-54,
Organism=Escherichia coli, GI1790083, Length=156, Percent_Identity=34.6153846153846, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17555908, Length=255, Percent_Identity=27.0588235294118, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=31.5151515151515, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27223; Mature: 27091

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKPAERLIYGFHAVLARLRHDPAGVLEIYLDLTRRDARARDLVHQARDSGVKLAQVEA
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHH
DRLGRLVGKAAKHQGVVARVKPVALTHSLDEVLESIEGPPLLVILDGVTDPHNLGAILRS
HHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
ADAFGAHAVIAPKDNAVGINATVEKVASGAADTVPYLMVTNLARTIEELKEHDIWVIAAD
CCCCCCEEEEECCCCCEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
MDGEQPLSGIDVRSGVAWVLGAEGAGIRRLVKQRCDQVARIPIGGTVESLNVSVSAALCL
CCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHEEHHHHHHHH
YETARQRGHASTRQ
HHHHHHHCCCCCCC
>Mature Secondary Structure 
SEKPAERLIYGFHAVLARLRHDPAGVLEIYLDLTRRDARARDLVHQARDSGVKLAQVEA
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHH
DRLGRLVGKAAKHQGVVARVKPVALTHSLDEVLESIEGPPLLVILDGVTDPHNLGAILRS
HHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
ADAFGAHAVIAPKDNAVGINATVEKVASGAADTVPYLMVTNLARTIEELKEHDIWVIAAD
CCCCCCEEEEECCCCCEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
MDGEQPLSGIDVRSGVAWVLGAEGAGIRRLVKQRCDQVARIPIGGTVESLNVSVSAALCL
CCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHEEHHHHHHHH
YETARQRGHASTRQ
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]