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The map label for this gene is rlmN

Identifier: 74316609

GI number: 74316609

Start: 619691

End: 620809

Strand: Direct

Name: rlmN

Synonym: Tbd_0591

Alternate gene names: 74316609

Gene position: 619691-620809 (Clockwise)

Preceding gene: 74316608

Following gene: 74316610

Centisome position: 21.3

GC content: 66.31

Gene sequence:

>1119_bases
ATGCCGCAGAATCTGCTCGATTTCGACCTCGCAGGACTGACCGCCTGGTTCGGCGAACGCGGCGAGAAGCCGTTTCGCGC
CCGCCAGGTGTTTCACTGGATCCACCAGGCGGGGGTGACCGATTTCGCGCAGATGACCGATATCGCCAAGAGTCTGCGCG
AGAAGTTGCAGAACGAGGCCGTCGTGCAGGCGCCGGCGATCAACTTCGCCCACCTGTCGGCCGACGGCACGCGCAAGTGG
CTGTTCGACGTCGGCGTCGGTAATGGCATTGAGACGGTATTCATTCCCGAGGACGATCGCGGAACGCTGTGCGTGTCGTC
GCAGGTCGGGTGCGCGCTCGAATGCACCTTCTGCTCGACCGGTCGGCAAGGCTTCAACCGCAACCTGACGGTCGCCGAGA
TCGTCGGTCAACTCTGGGTCGCGCAGCACAGCCTGAAGCGCGAGCCTAATCGCACCGCGTCCGACCACGGCGCAGGCGAG
ATCGCCGAACGTCCGGTCACCAACGTGGTGATGATGGGCATGGGCGAGCCGCTCGCGAACTTCGAGAACGTCGTGACCGC
ACTCGGCGTCATGCTCGACGACCATGCCTACGGGCTGTCGCGGCGGCGCGTGACCGTGTCGACCTCGGGCCTCGTTCCGG
CGATGGACCGGCTGGCCGAGCGCTGCCCGGTGGCACTCGCGGTGAGCCTGCACGCACCGAACGACGCGCTGCGCGACCAG
ATCGTGCCGATCAACCGGAAATACCCGCTGGCGGAATTGATGGCGGCCTGCCGGCGCTACCTCGTGCATGCGCCGCGCGA
CTTCATCACCTTCGAGTACGTGATGCTGGCCGGCGTGAACGACCAGCCCGAGCATGCGCGCCAGTTGATCGCGCTCACGC
GCGACGTGCCGTGCAAGTTCAATCTCATCCCGTTCAATCCGTTCCCGGATTCGGGCTACGAAAAACCGCGCGCGGAAGCG
ATGCGCGTGTTCCGCGAGATCCTGCAGGACGCCGGCTACGTCGTGACGACCCGCAAGACCCGGGGCGACGACATCGACGC
GGCCTGCGGGCAACTCGCCGGACGCGTGGCGGACAGGAGCGGTCGTGTGATGAAGCGGGTGCACAGGGAGGCGGCGTGA

Upstream 100 bases:

>100_bases
CGCCAACGCCGTTCACGGTTCCGACGCGCCCGAAACGGCGGCAGTCGAGATTGCCTACTTCTTCCCGGCGAGCGAAGTCT
ACGCCGGACGCTGAGCGCGC

Downstream 100 bases:

>100_bases
CGTGGTGAAACCGGAGCTGAGAAAGTGGATCGTCGTCACGGCAGTCGCGCTCGCCGGCTGCGCGGGCCAGCCCGGTGGCG
GCGGCATCGGCGCGCCCGAT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MPQNLLDFDLAGLTAWFGERGEKPFRARQVFHWIHQAGVTDFAQMTDIAKSLREKLQNEAVVQAPAINFAHLSADGTRKW
LFDVGVGNGIETVFIPEDDRGTLCVSSQVGCALECTFCSTGRQGFNRNLTVAEIVGQLWVAQHSLKREPNRTASDHGAGE
IAERPVTNVVMMGMGEPLANFENVVTALGVMLDDHAYGLSRRRVTVSTSGLVPAMDRLAERCPVALAVSLHAPNDALRDQ
IVPINRKYPLAELMAACRRYLVHAPRDFITFEYVMLAGVNDQPEHARQLIALTRDVPCKFNLIPFNPFPDSGYEKPRAEA
MRVFREILQDAGYVVTTRKTRGDDIDAACGQLAGRVADRSGRVMKRVHREAA

Sequences:

>Translated_372_residues
MPQNLLDFDLAGLTAWFGERGEKPFRARQVFHWIHQAGVTDFAQMTDIAKSLREKLQNEAVVQAPAINFAHLSADGTRKW
LFDVGVGNGIETVFIPEDDRGTLCVSSQVGCALECTFCSTGRQGFNRNLTVAEIVGQLWVAQHSLKREPNRTASDHGAGE
IAERPVTNVVMMGMGEPLANFENVVTALGVMLDDHAYGLSRRRVTVSTSGLVPAMDRLAERCPVALAVSLHAPNDALRDQ
IVPINRKYPLAELMAACRRYLVHAPRDFITFEYVMLAGVNDQPEHARQLIALTRDVPCKFNLIPFNPFPDSGYEKPRAEA
MRVFREILQDAGYVVTTRKTRGDDIDAACGQLAGRVADRSGRVMKRVHREAA
>Mature_371_residues
PQNLLDFDLAGLTAWFGERGEKPFRARQVFHWIHQAGVTDFAQMTDIAKSLREKLQNEAVVQAPAINFAHLSADGTRKWL
FDVGVGNGIETVFIPEDDRGTLCVSSQVGCALECTFCSTGRQGFNRNLTVAEIVGQLWVAQHSLKREPNRTASDHGAGEI
AERPVTNVVMMGMGEPLANFENVVTALGVMLDDHAYGLSRRRVTVSTSGLVPAMDRLAERCPVALAVSLHAPNDALRDQI
VPINRKYPLAELMAACRRYLVHAPRDFITFEYVMLAGVNDQPEHARQLIALTRDVPCKFNLIPFNPFPDSGYEKPRAEAM
RVFREILQDAGYVVTTRKTRGDDIDAACGQLAGRVADRSGRVMKRVHREAA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=372, Percent_Identity=54.3010752688172, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_THIDA (Q3SL73)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314349.1
- STRING:   Q3SL73
- GeneID:   3673701
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0591
- NMPDR:   fig|292415.3.peg.393
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q3SL73
- BioCyc:   TDEN292415:TBD_0591-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41199; Mature: 41067

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQNLLDFDLAGLTAWFGERGEKPFRARQVFHWIHQAGVTDFAQMTDIAKSLREKLQNEA
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
VVQAPAINFAHLSADGTRKWLFDVGVGNGIETVFIPEDDRGTLCVSSQVGCALECTFCST
EEECCCCCEEEECCCCCEEEEEEECCCCCCEEEEECCCCCCCEEEECCCCCEEEEEEECC
GRQGFNRNLTVAEIVGQLWVAQHSLKREPNRTASDHGAGEIAERPVTNVVMMGMGEPLAN
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHEEEECCCCCHHH
FENVVTALGVMLDDHAYGLSRRRVTVSTSGLVPAMDRLAERCPVALAVSLHAPNDALRDQ
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEEEEECCCHHHHCC
IVPINRKYPLAELMAACRRYLVHAPRDFITFEYVMLAGVNDQPEHARQLIALTRDVPCKF
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHEEEHEEECCCCCHHHHHHHHHHHCCCCCEE
NLIPFNPFPDSGYEKPRAEAMRVFREILQDAGYVVTTRKTRGDDIDAACGQLAGRVADRS
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
GRVMKRVHREAA
CHHHHHHHHHCC
>Mature Secondary Structure 
PQNLLDFDLAGLTAWFGERGEKPFRARQVFHWIHQAGVTDFAQMTDIAKSLREKLQNEA
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
VVQAPAINFAHLSADGTRKWLFDVGVGNGIETVFIPEDDRGTLCVSSQVGCALECTFCST
EEECCCCCEEEECCCCCEEEEEEECCCCCCEEEEECCCCCCCEEEECCCCCEEEEEEECC
GRQGFNRNLTVAEIVGQLWVAQHSLKREPNRTASDHGAGEIAERPVTNVVMMGMGEPLAN
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHEEEECCCCCHHH
FENVVTALGVMLDDHAYGLSRRRVTVSTSGLVPAMDRLAERCPVALAVSLHAPNDALRDQ
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEEEEECCCHHHHCC
IVPINRKYPLAELMAACRRYLVHAPRDFITFEYVMLAGVNDQPEHARQLIALTRDVPCKF
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHEEEHEEECCCCCHHHHHHHHHHHCCCCCEE
NLIPFNPFPDSGYEKPRAEAMRVFREILQDAGYVVTTRKTRGDDIDAACGQLAGRVADRS
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
GRVMKRVHREAA
CHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA