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The map label for this gene is folE [H]

Identifier: 74316499

GI number: 74316499

Start: 503097

End: 503744

Strand: Direct

Name: folE [H]

Synonym: Tbd_0481

Alternate gene names: 74316499

Gene position: 503097-503744 (Clockwise)

Preceding gene: 74316498

Following gene: 74316500

Centisome position: 17.29

GC content: 70.99

Gene sequence:

>648_bases
ATGGCCGACGCCTGCGACCACGACGGCTGCCACACGCCGACGCGCGAAGGCGGCGTCGGCATCCCGCAGACCGGGGCTTT
CGACCCGGTCGCGTTCGAGCGCGCGGTCAACGACATGCTCGCGGCGTGCGGCGTCGCCGCGGACTCGGTGCATACCGGGC
GCACCGCGCAGCGGGTGCGCGAACTGTGGCAGAAGCGCCTGCTTGGCGGTTATGCCGTGACGCCCGCCGACGCACTCGGG
GAAGGGTTTGCCGACGACCGCGACGACATGGTCGTGGTGCGCGGCATCGCGGTTCACGGGGTATGTCCGCACCACCTCGT
CCCGTTTCGCGGCGTCGCGCATGTCGCCTACGTCCCGGGCGGCCGCTTGCACGGCTTCGGGCGAATCGCGCGCATGGTCG
ACGCGATCGGCCACCGCTTCACCTACCAGGAGTGGATGACGCGCGACATCGCCGAAGCGCTGACGACGCACGGGCAGGCG
CGCGGGGCGGCATGCATCATCGAAGCCGAGCAACTGTGCCTGCTGCTCGGCGAGGACCGGCGCGGCGACGAGCGCGTCGT
CACCCAGGCATTCACCGGCTGCTTCCGCGACAGCGACCAGTTGCGCAACGAATTGCTGCGCGCGACGAGCCAGCGCGAAG
TGCGCTGA

Upstream 100 bases:

>100_bases
GCTATGCCGCCCAGTTCGGCCTGCCGCTCAAGTACGACAACGCGGCGGACAAGGACTCGTGGATACGCACGCTCGAATTC
CTCAAGGTGACCCTGCACTG

Downstream 100 bases:

>100_bases
CAGCCGGCGACGCCGAACCGACCGCACGCCAGCGGTTTGCTTGACACATACCAACCGGTATGTATGATCAAGTCATGCAT
GCCTCCGCCAAGACGCCCGA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MADACDHDGCHTPTREGGVGIPQTGAFDPVAFERAVNDMLAACGVAADSVHTGRTAQRVRELWQKRLLGGYAVTPADALG
EGFADDRDDMVVVRGIAVHGVCPHHLVPFRGVAHVAYVPGGRLHGFGRIARMVDAIGHRFTYQEWMTRDIAEALTTHGQA
RGAACIIEAEQLCLLLGEDRRGDERVVTQAFTGCFRDSDQLRNELLRATSQREVR

Sequences:

>Translated_215_residues
MADACDHDGCHTPTREGGVGIPQTGAFDPVAFERAVNDMLAACGVAADSVHTGRTAQRVRELWQKRLLGGYAVTPADALG
EGFADDRDDMVVVRGIAVHGVCPHHLVPFRGVAHVAYVPGGRLHGFGRIARMVDAIGHRFTYQEWMTRDIAEALTTHGQA
RGAACIIEAEQLCLLLGEDRRGDERVVTQAFTGCFRDSDQLRNELLRATSQREVR
>Mature_214_residues
ADACDHDGCHTPTREGGVGIPQTGAFDPVAFERAVNDMLAACGVAADSVHTGRTAQRVRELWQKRLLGGYAVTPADALGE
GFADDRDDMVVVRGIAVHGVCPHHLVPFRGVAHVAYVPGGRLHGFGRIARMVDAIGHRFTYQEWMTRDIAEALTTHGQAR
GAACIIEAEQLCLLLGEDRRGDERVVTQAFTGCFRDSDQLRNELLRATSQREVR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=138, Percent_Identity=34.0579710144928, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI4503949, Length=138, Percent_Identity=34.0579710144928, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI66932970, Length=103, Percent_Identity=36.8932038834951, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI66932972, Length=101, Percent_Identity=37.6237623762376, Blast_Score=81, Evalue=8e-16,
Organism=Escherichia coli, GI1788476, Length=171, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560486, Length=144, Percent_Identity=34.0277777777778, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321706, Length=132, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24656782, Length=144, Percent_Identity=34.0277777777778, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24656791, Length=144, Percent_Identity=34.0277777777778, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24656796, Length=144, Percent_Identity=34.0277777777778, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 23431; Mature: 23300

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADACDHDGCHTPTREGGVGIPQTGAFDPVAFERAVNDMLAACGVAADSVHTGRTAQRVR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ELWQKRLLGGYAVTPADALGEGFADDRDDMVVVRGIAVHGVCPHHLVPFRGVAHVAYVPG
HHHHHHHHCCCCCCCHHHHCCCCCCCCCCEEEEEEEHHCCCCCCCCCCCCCCEEEEECCC
GRLHGFGRIARMVDAIGHRFTYQEWMTRDIAEALTTHGQARGAACIIEAEQLCLLLGEDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCC
RGDERVVTQAFTGCFRDSDQLRNELLRATSQREVR
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADACDHDGCHTPTREGGVGIPQTGAFDPVAFERAVNDMLAACGVAADSVHTGRTAQRVR
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ELWQKRLLGGYAVTPADALGEGFADDRDDMVVVRGIAVHGVCPHHLVPFRGVAHVAYVPG
HHHHHHHHCCCCCCCHHHHCCCCCCCCCCEEEEEEEHHCCCCCCCCCCCCCCEEEEECCC
GRLHGFGRIARMVDAIGHRFTYQEWMTRDIAEALTTHGQARGAACIIEAEQLCLLLGEDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCC
RGDERVVTQAFTGCFRDSDQLRNELLRATSQREVR
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]