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The map label for this gene is yghX [H]

Identifier: 74316498

GI number: 74316498

Start: 502315

End: 503097

Strand: Direct

Name: yghX [H]

Synonym: Tbd_0480

Alternate gene names: 74316498

Gene position: 502315-503097 (Clockwise)

Preceding gene: 74316497

Following gene: 74316499

Centisome position: 17.26

GC content: 64.11

Gene sequence:

>783_bases
ATGAAAACTGCCCTTGCGTTGTCGCTGTTGATGTTCACGTCTTCCGCCCTCGCTGCCGTGATCGGCAAGGACATCAGCTA
CAAGGCCGGCGATACCGTGATGAAGGGATTTCTCGCCTATGACGACGCAGTCGCGGGCAAGCGTGCGGGCGTGCTCGTGG
TGCCGGAATGGTGGGGCGCGAACGACTACCCGAGAAAGCGTGCGCGCATGCTCGCCGAGGCGGGCTACGTCGCACTCGTC
GTCGACATGTACGGCGAGGGCCAGGTCACCGACAATCCCAAGGATGCGGGCGCGCTCGCGGGTAACGTGAACAAGAATCC
GCAGCTGGCCTACGCGCGCGTCGACGCCGCGCGCGAATTTCTCGACCGGCAGCCGCACGTCAAGAAAGGTGAAACCGCTG
CCCTCGGCTACTGCTTCGGCGGCGGCGTCGTGCTCAACGTCGTGCGCGCGGGCATGCCGCTCAAGTCGGCCGTGAGCTAC
CACGGCGTGCTCGCGACCGACATCGCGGTGAAGCCGGGTGACATCGAGGCGAAGCTGCGTGTCTTCCATGGCGAAGCCGA
TCCGATCGTTCCGCCCGAGCAGGTCGAGGCGTTCAAGACCGAGATGGACAACGCCAAGGCCGACTACATGTTCGTCTCCT
ATCCCGGCGTCAAGCACACCTTCACCAACCGCGAGGCCGACAGCTATGCCGCCCAGTTCGGCCTGCCGCTCAAGTACGAC
AACGCGGCGGACAAGGACTCGTGGATACGCACGCTCGAATTCCTCAAGGTGACCCTGCACTGA

Upstream 100 bases:

>100_bases
CAAAAGACGCGCCCCTGCCGCGGTTGTTATCCCGCGGTGCGGCGCGGTATGCTTTTCGTTCACTCAATCTGGCCGCCTGC
GGTCACCCCAGGAGATGCCC

Downstream 100 bases:

>100_bases
TGGCCGACGCCTGCGACCACGACGGCTGCCACACGCCGACGCGCGAAGGCGGCGTCGGCATCCCGCAGACCGGGGCTTTC
GACCCGGTCGCGTTCGAGCG

Product: dienelactone hydrolase family protein

Products: oxohex-2-enedioate [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKTALALSLLMFTSSALAAVIGKDISYKAGDTVMKGFLAYDDAVAGKRAGVLVVPEWWGANDYPRKRARMLAEAGYVALV
VDMYGEGQVTDNPKDAGALAGNVNKNPQLAYARVDAAREFLDRQPHVKKGETAALGYCFGGGVVLNVVRAGMPLKSAVSY
HGVLATDIAVKPGDIEAKLRVFHGEADPIVPPEQVEAFKTEMDNAKADYMFVSYPGVKHTFTNREADSYAAQFGLPLKYD
NAADKDSWIRTLEFLKVTLH

Sequences:

>Translated_260_residues
MKTALALSLLMFTSSALAAVIGKDISYKAGDTVMKGFLAYDDAVAGKRAGVLVVPEWWGANDYPRKRARMLAEAGYVALV
VDMYGEGQVTDNPKDAGALAGNVNKNPQLAYARVDAAREFLDRQPHVKKGETAALGYCFGGGVVLNVVRAGMPLKSAVSY
HGVLATDIAVKPGDIEAKLRVFHGEADPIVPPEQVEAFKTEMDNAKADYMFVSYPGVKHTFTNREADSYAAQFGLPLKYD
NAADKDSWIRTLEFLKVTLH
>Mature_260_residues
MKTALALSLLMFTSSALAAVIGKDISYKAGDTVMKGFLAYDDAVAGKRAGVLVVPEWWGANDYPRKRARMLAEAGYVALV
VDMYGEGQVTDNPKDAGALAGNVNKNPQLAYARVDAAREFLDRQPHVKKGETAALGYCFGGGVVLNVVRAGMPLKSAVSY
HGVLATDIAVKPGDIEAKLRVFHGEADPIVPPEQVEAFKTEMDNAKADYMFVSYPGVKHTFTNREADSYAAQFGLPLKYD
NAADKDSWIRTLEFLKVTLH

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994982, Length=219, Percent_Identity=28.7671232876712, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532655, Length=218, Percent_Identity=36.697247706422, Blast_Score=120, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI193204305, Length=213, Percent_Identity=35.6807511737089, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17536419, Length=245, Percent_Identity=31.4285714285714, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17532653, Length=216, Percent_Identity=35.6481481481481, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17534829, Length=147, Percent_Identity=35.3741496598639, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536415, Length=207, Percent_Identity=27.0531400966184, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 28165; Mature: 28165

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALALSLLMFTSSALAAVIGKDISYKAGDTVMKGFLAYDDAVAGKRAGVLVVPEWWGA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
NDYPRKRARMLAEAGYVALVVDMYGEGQVTDNPKDAGALAGNVNKNPQLAYARVDAAREF
CCCHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCEECCCCCCCCEEHHHHHHHHHH
LDRQPHVKKGETAALGYCFGGGVVLNVVRAGMPLKSAVSYHGVLATDIAVKPGDIEAKLR
HCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCHHHHHHHCCEEEEEEEECCCCCCEEEE
VFHGEADPIVPPEQVEAFKTEMDNAKADYMFVSYPGVKHTFTNREADSYAAQFGLPLKYD
EEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEECCCCCCHHHHHHHCCCEEEC
NAADKDSWIRTLEFLKVTLH
CCCCHHHHHHHHHHHHEECC
>Mature Secondary Structure
MKTALALSLLMFTSSALAAVIGKDISYKAGDTVMKGFLAYDDAVAGKRAGVLVVPEWWGA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
NDYPRKRARMLAEAGYVALVVDMYGEGQVTDNPKDAGALAGNVNKNPQLAYARVDAAREF
CCCHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCEECCCCCCCCEEHHHHHHHHHH
LDRQPHVKKGETAALGYCFGGGVVLNVVRAGMPLKSAVSYHGVLATDIAVKPGDIEAKLR
HCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCHHHHHHHCCEEEEEEEECCCCCCEEEE
VFHGEADPIVPPEQVEAFKTEMDNAKADYMFVSYPGVKHTFTNREADSYAAQFGLPLKYD
EEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEECCCCCCHHHHHHHCCCEEEC
NAADKDSWIRTLEFLKVTLH
CCCCHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]